Restriction map for
pFN21AB8777
(Circular) (Six-base) MAPSORT of: pFN21AB8777.seq Check: 4199 from: 1 to: 5476
pFN21AB8777 5476 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2271 2271
Size: 5476
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3286 278
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2819 1937
Size: 882 4594
Acc65I G'GTAC_C
Cuts at: 2797 2797
Size: 5476
AclI AA'CG_TT
Cuts at: 1563 3603 3976 1563
Size: 2040 373 3063
Fragments arranged by size:
3063 2040 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2153 2231 2504 2744 3539 4923 5191 1632
Size: 521 78 273 240 795 1384 268 1917
Fragments arranged by size:
1917 1384 795 521 273 268 240 78
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5279
AflIII A'CryG_T
Cuts at: 1203 4375 5352 1203
Size: 3172 977 1327
Fragments arranged by size:
3172 1327 977
AgeI A'CCGG_T
Cuts at: 4307 4307
Size: 5476
AhdI GACnn_n'nnGTC
Cuts at: 4205 4205
Size: 5476
AleI CACnn'nnGTG
Cuts at: 1166 2528 2615 1166
Size: 1362 87 4027
Fragments arranged by size:
4027 1362 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5476
AlwNI CAG_nnn'CTG
Cuts at: 2138 2263 2569 2589 3219 4791 2138
Size: 125 306 20 630 1572 2823
Fragments arranged by size:
2823 1572 630 306 125 20
ApaI G_GGCC'C
Cuts at: 2775 2775
Size: 5476
ApaLI G'TGCA_C
Cuts at: 3533 4689 3533
Size: 1156 4320
ApoI r'AATT_y
Cuts at: 1493 2785 3011 3163 5272 1493
Size: 1292 226 152 2109 1697
Fragments arranged by size:
2109 1697 1292 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4030 160
Size: 872 2998 1606
Fragments arranged by size:
2998 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
AvaI C'yCGr_G
Cuts at: 1870 1943 2052 2180 2767 2801 1870
Size: 73 109 128 587 34 4545
Fragments arranged by size:
4545 587 128 109 73 34
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2414 2447 2414
Size: 33 5443
BamHI G'GATC_C
Cuts at: 2385 2806 3173 2385
Size: 421 367 4688
Fragments arranged by size:
4688 421 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 2093 2118 2579 2709
Size: 325 217 627 222 84 25 461 130
Cuts at: 2709 2797 4252 618
Size: 88 1455 1842
Fragments arranged by size:
1842 1455 627 461 325 222 217 130
88 84 25
BanII G_rGCy'C
Cuts at: 1391 2775 2795 1391
Size: 1384 20 4072
Fragments arranged by size:
4072 1384 20
BbeI G_GCGC'C
Cuts at: 2097 2097
Size: 5476
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2140 928
Size: 971 241 4264
Fragments arranged by size:
4264 971 241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3667 3701 1903
Size: 34 1730 34 3678
Fragments arranged by size:
3678 1730 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3368 4578 3368
Size: 1210 4266
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5476
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2306 4087 136
Size: 107 122 71 631 605 634 1781 1525
Fragments arranged by size:
1781 1525 634 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2229 5471 2229
Size: 3242 2234
BlpI GC'TnA_GC
Cuts at: 2893 2893
Size: 5476
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2123 2775 3537 4693 1790
Size: 222 111 652 762 1156 2573
Fragments arranged by size:
2573 1156 762 652 222 111
BmgBI CAC'GTC
Cuts at: 1318 2617 1318
Size: 1299 4177
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3207 4165 442
Size: 416 53 83 399 24 1790 958 1753
Fragments arranged by size:
1790 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2735 4136 1239
Size: 1496 1401 2579
Fragments arranged by size:
2579 1496 1401
Bpu10I CC'TnA_GC
Cuts at: 2273 2273
Size: 5476
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3601 4466 4764 3601
Size: 865 298 4313
Fragments arranged by size:
4313 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2050 4139 882
Size: 617 551 2089 2219
Fragments arranged by size:
2219 2089 617 551
BsaAI yAC'GTr
Cuts at: 493 1204 3267 493
Size: 711 2063 2702
Fragments arranged by size:
2702 2063 711
BsaBI GATnn'nnATC
Cuts at: 5470 5470
Size: 5476
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 2397 3283 3665
Size: 53 83 186 936 561 303 886 382
Cuts at: 3665 4300 275
Size: 635 1451
Fragments arranged by size:
1451 936 886 635 561 382 303 186
83 53
BsaWI w'CCGG_w
Cuts at: 3909 4307 4581 4728 5139 3909
Size: 398 274 147 411 4246
Fragments arranged by size:
4246 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2216 2246 2459 2489 1178
Size: 30 165 30 813 30 213 30 4165
Fragments arranged by size:
4165 813 213 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2552 1186
Size: 877 489 4110
Fragments arranged by size:
4110 877 489
BseYI C'CCAG_C
Cuts at: 2735 4679 5175 2735
Size: 1944 496 3036
Fragments arranged by size:
3036 1944 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 2249 2429 2552 1163
Size: 573 333 180 180 123 4087
Fragments arranged by size:
4087 573 333 180 180 123
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2926 3687 4715 1540
Size: 275 186 925 761 1028 2301
Fragments arranged by size:
2301 1028 925 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2208 3537 3622 4693 2208
Size: 1329 85 1071 2991
Fragments arranged by size:
2991 1329 1071 85
BsmI GAATG_Cn'
Cuts at: 2987 3080 2987
Size: 93 5383
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2123 2208 2775 2795 3537 3622
Size: 399 222 111 85 567 20 742 85
Cuts at: 3622 4693 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 567 399 222 111 85
85 20
BspEI T'CCGG_A
Cuts at: 5139 5139
Size: 5476
BspHI T'CATG_A
Cuts at: 3365 5095 3365
Size: 1730 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2271 2831 844
Size: 1427 560 3489
Fragments arranged by size:
3489 1427 560
BsrBI CCG'CTC
Cuts at: 1394 1823 3363 1394
Size: 429 1540 3507
Fragments arranged by size:
3507 1540 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3971 4145 65
Size: 1183 2723 174 1396
Fragments arranged by size:
2723 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2115 2268 4120 4307 1700
Size: 110 305 153 1852 187 2869
Fragments arranged by size:
2869 1852 305 187 153 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5476
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5476
BssSI C'ACGA_G
Cuts at: 3536 4548 3536
Size: 1012 4464
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5476
BstBI TT'CG_AA
Cuts at: 4280 4280
Size: 5476
BstEII G'GTnAC_C
Cuts at: 1184 2629 1184
Size: 1445 4031
BstXI CCAn_nnnn'nTGG
Cuts at: 2617 2617
Size: 5476
BstYI r'GATC_y
Cuts at: 1972 2229 2284 2385 2806 3173 3558 3575 5027
Size: 257 55 101 421 367 385 17 1452
Cuts at: 5027 5113 5125 5471 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 421 385 367 346 257 101
86 55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2652 513
Size: 552 939 648 3337
Fragments arranged by size:
3337 939 648 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4737
Fragments arranged by size:
4737 683 56
BtsI GCAGTG_nn'
Cuts at: 2446 2992 3786 3806 2446
Size: 546 794 20 4116
Fragments arranged by size:
4116 794 546 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5441
DraI TTT'AAA
Cuts at: 2780 2780
Size: 5476
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4483 809
Size: 507 3167 1802
Fragments arranged by size:
3167 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2337 2619
Size: 54 1425 50 275 18 363 144 282
Cuts at: 2619 2923 3812 5441 8
Size: 304 889 1629 43
Fragments arranged by size:
1629 1425 889 363 304 282 275 144
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2923 1537
Size: 275 1111 4090
Fragments arranged by size:
4090 1111 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 2081 3406 1353
Size: 719 9 1325 3423
Fragments arranged by size:
3423 1325 719 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2308 4063 4437 4583 387
Size: 1064 138 719 1755 374 146 1280
Fragments arranged by size:
1755 1280 1064 719 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2231 2504 2735 2744 3539 4136
Size: 393 521 78 273 231 9 795 597
Cuts at: 4136 4923 5191 1239
Size: 787 268 1524
Fragments arranged by size:
1524 795 787 597 521 393 273 268
231 78 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2171 2465 2171
Size: 294 5182
EcoO109I rG'GnC_Cy
Cuts at: 2164 2176 2250 2344 2470 2755 2771 2164
Size: 12 74 94 126 285 16 4869
Fragments arranged by size:
4869 285 126 94 74 16 12
EcoRI G'AATT_C
Cuts at: 2785 3163 2785
Size: 378 5098
FspI TGC'GCA
Cuts at: 3982 3982
Size: 5476
HaeII r_GCGC'y
Cuts at: 1678 2097 2418 2742 4623 1678
Size: 419 321 324 1881 2531
Fragments arranged by size:
2531 1881 419 324 321
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2483 2515 4117 4149 4191
Size: 32 135 32 911 32 1602 32 42
Cuts at: 4191 4223 1373
Size: 32 2626
Fragments arranged by size:
2626 1602 911 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2820 3066 669
Size: 988 281 882 246 3079
Fragments arranged by size:
3079 988 882 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5476
HpaI GTT'AAC
Cuts at: 3066 3066
Size: 5476
KasI G'GCGC_C
Cuts at: 2093 2093
Size: 5476
KpnI G_GTAC'C
Cuts at: 2801 2801
Size: 5476
MfeI C'AATT_G
Cuts at: 3075 3075
Size: 5476
MluI A'CGCG_T
Cuts at: 5352 5352
Size: 5476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4590 4774 1305
Size: 3285 184 2007
Fragments arranged by size:
3285 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2621 10
Size: 54 1425 343 789 2865
Fragments arranged by size:
2865 1425 789 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 2615 3434 3793 3952 518
Size: 648 1362 87 819 359 159 2042
Fragments arranged by size:
2042 1362 819 648 359 159 87
MspA1I CmG'CkG
Cuts at: 1606 2892 3569 4717 4962 1606
Size: 1286 677 1148 245 2120
Fragments arranged by size:
2120 1286 1148 677 245
NarI GG'CG_CC
Cuts at: 2094 2094
Size: 5476
NcoI C'CATG_G
Cuts at: 513 1065 2004 2652 513
Size: 552 939 648 3337
Fragments arranged by size:
3337 939 648 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5476
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5476
NotI GC'GGCC_GC
Cuts at: 2923 2923
Size: 5476
NspI r_CATG'y
Cuts at: 1132 1229 2834 4379 1132
Size: 97 1605 1545 2229
Fragments arranged by size:
2229 1605 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2401 1348
Size: 1053 4423
PciI A'CATG_T
Cuts at: 4375 4375
Size: 5476
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5476
PfoI T'CCnGG_A
Cuts at: 2241 2241
Size: 5476
PmeI GTTT'AAAC
Cuts at: 2780 2780
Size: 5476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 5058 5090 3545
Size: 32 1481 32 3931
Fragments arranged by size:
3931 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2176 2755 2176
Size: 579 4897
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5476
PsiI TTA'TAA
Cuts at: 3046 3046
Size: 5476
PspOMI G'GGCC_C
Cuts at: 2771 2771
Size: 5476
PstI C_TGCA'G
Cuts at: 830 2156 2466 2594 2828 830
Size: 1326 310 128 234 3478
Fragments arranged by size:
3478 1326 310 234 128
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5476
SalI G'TCGA_C
Cuts at: 1936 2818 1936
Size: 882 4594
SbfI CC_TGCA'GG
Cuts at: 2466 2828 2466
Size: 362 5114
ScaI AGT'ACT
Cuts at: 3724 5436 3724
Size: 1712 3764
SfcI C'TryA_G
Cuts at: 826 1045 2152 2462 2590 2824 3959 4640 4831
Size: 219 1107 310 128 234 1135 681 191
Cuts at: 4831 826
Size: 1471
Fragments arranged by size:
1471 1135 1107 681 310 234 219 191
128
SfoI GGC'GCC
Cuts at: 2095 2095
Size: 5476
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5476
SmaI CCC'GGG
Cuts at: 1872 2054 2769 2803 1872
Size: 182 715 34 4545
Fragments arranged by size:
4545 715 182 34
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3580 4481 4743 820
Size: 197 926 1637 901 262 1553
Fragments arranged by size:
1637 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3267 493
Size: 2774 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5476
SphI G_CATG'C
Cuts at: 1132 2834 1132
Size: 1702 3774
SspI AAT'ATT
Cuts at: 5 52 3400 5
Size: 47 3348 2081
Fragments arranged by size:
3348 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5476
StyI C'CwwG_G
Cuts at: 513 1065 2004 2391 2652 2751 2915 513
Size: 552 939 387 261 99 164 3074
Fragments arranged by size:
3074 939 552 387 261 164 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3503 3520 3673 3858 4303 325
Size: 1255 1923 17 153 185 445 1498
Fragments arranged by size:
1923 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2673 3722 5434
Size: 274 80 33 51 1443 696 1049 1712
Cuts at: 5434 96
Size: 138
Fragments arranged by size:
1712 1443 1049 696 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2459 2491 5436 5468 2459
Size: 32 2945 32 2467
Fragments arranged by size:
2945 2467 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5476
XbaI T'CTAG_A
Cuts at: 2812 2812
Size: 5476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2398 2398
Size: 5476
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5476
XmaI C'CCGG_G
Cuts at: 1870 2052 2767 2801 1870
Size: 182 715 34 4545
Fragments arranged by size:
4545 715 182 34
XmnI GAAnn'nnTTC
Cuts at: 3605 3605
Size: 5476
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3284 276
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI