Restriction map for
pFN21AB8776
(Circular) (Six-base) MAPSORT of: pFN21AB8776.seq Check: 2287 from: 1 to: 6328
pFN21AB8776 6328 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4138 278
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3671 1937
Size: 1734 4594
Acc65I G'GTAC_C
Cuts at: 3649 3649
Size: 6328
AclI AA'CG_TT
Cuts at: 1563 4455 4828 1563
Size: 2892 373 3063
Fragments arranged by size:
3063 2892 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3379 4391 5775 6043 1632
Size: 1747 1012 1384 268 1917
Fragments arranged by size:
1917 1747 1384 1012 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6131
AflIII A'CryG_T
Cuts at: 1203 5227 6204 1203
Size: 4024 977 1327
Fragments arranged by size:
4024 1327 977
AgeI A'CCGG_T
Cuts at: 3017 5159 3017
Size: 2142 4186
AhdI GACnn_n'nnGTC
Cuts at: 5057 5057
Size: 6328
AleI CACnn'nnGTG
Cuts at: 1166 3018 1166
Size: 1852 4476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2241 1184
Size: 1057 5271
AlwNI CAG_nnn'CTG
Cuts at: 3408 4071 5643 3408
Size: 663 1572 4093
Fragments arranged by size:
4093 1572 663
ApaLI G'TGCA_C
Cuts at: 4385 5541 4385
Size: 1156 5172
ApoI r'AATT_y
Cuts at: 1493 2029 2539 2841 2899 3429 3637 3863 4015
Size: 536 510 302 58 530 208 226 152
Cuts at: 4015 6124 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 536 530 510 302 226 208
152 58
AseI AT'TA_AT
Cuts at: 160 1032 4882 160
Size: 872 3850 1606
Fragments arranged by size:
3850 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
AvaI C'yCGr_G
Cuts at: 1870 1943 3653 1870
Size: 73 1710 4545
Fragments arranged by size:
4545 1710 73
BamHI G'GATC_C
Cuts at: 3658 4025 3658
Size: 367 5961
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3336 3649 5104 618
Size: 325 217 627 1549 313 1455 1842
Fragments arranged by size:
1842 1549 1455 627 325 313 217
BanII G_rGCy'C
Cuts at: 1391 2567 3575 3647 1391
Size: 1176 1008 72 4072
Fragments arranged by size:
4072 1176 1008 72
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5357
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4519 4553 1903
Size: 34 2582 34 3678
Fragments arranged by size:
3678 2582 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4220 5430 4220
Size: 1210 5118
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6328
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4939 136
Size: 107 122 71 631 605 3267 1525
Fragments arranged by size:
3267 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6323 6323
Size: 6328
BlpI GC'TnA_GC
Cuts at: 2818 3745 2818
Size: 927 5401
Bme1580I G_kGCm'C
Cuts at: 1790 4389 5545 1790
Size: 2599 1156 2573
Fragments arranged by size:
2599 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6328
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4059 5017 442
Size: 416 53 83 399 24 2642 958 1753
Fragments arranged by size:
2642 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 2619 3123 4988 1239
Size: 947 433 504 1865 2579
Fragments arranged by size:
2579 1865 947 504 433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2164 4453 5318 5616 2164
Size: 2289 865 298 2876
Fragments arranged by size:
2876 2289 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3437 4991 882
Size: 617 1938 1554 2219
Fragments arranged by size:
2219 1938 1554 617
BsaAI yAC'GTr
Cuts at: 493 1204 4119 493
Size: 711 2915 2702
Fragments arranged by size:
2915 2702 711
BsaBI GATnn'nnATC
Cuts at: 6322 6322
Size: 6328
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4135 4517 5152 275
Size: 53 83 186 936 2602 382 635 1451
Fragments arranged by size:
2602 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2681 3017 4761 5159 5433 5580 5991 2681
Size: 336 1744 398 274 147 411 3018
Fragments arranged by size:
3018 1744 411 398 336 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6103
Fragments arranged by size:
6103 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6328
BseYI C'CCAG_C
Cuts at: 2327 3081 3533 5531 6027 2327
Size: 754 452 1998 496 2628
Fragments arranged by size:
2628 1998 754 496 452
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3144 1163
Size: 573 1408 4347
Fragments arranged by size:
4347 1408 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3778 4539 5567 1540
Size: 275 186 1777 761 1028 2301
Fragments arranged by size:
2301 1777 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2567 3575 3601 4389 4474 5545 2567
Size: 1008 26 788 85 1071 3350
Fragments arranged by size:
3350 1071 1008 788 85 26
BsmI GAATG_Cn'
Cuts at: 2789 2873 3839 3932 2789
Size: 84 966 93 5185
Fragments arranged by size:
5185 966 93 84
BsmBI CGTCTCn'nnnn_
Cuts at: 2253 2253
Size: 6328
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2567 3575 3601 3647 4389 4474 5545
Size: 399 777 1008 26 46 742 85 1071
Cuts at: 5545 1391
Size: 2174
Fragments arranged by size:
2174 1071 1008 777 742 399 85 46
26
BspEI T'CCGG_A
Cuts at: 5991 5991
Size: 6328
BspHI T'CATG_A
Cuts at: 4217 5947 4217
Size: 1730 4598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3683 844
Size: 2839 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4215 1394
Size: 429 2392 3507
Fragments arranged by size:
3507 2392 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4823 4997 65
Size: 1183 3575 174 1396
Fragments arranged by size:
3575 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3017 3537 4972 5159 1700
Size: 110 1207 520 1435 187 2869
Fragments arranged by size:
2869 1435 1207 520 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6328
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6328
BssSI C'ACGA_G
Cuts at: 4388 5400 4388
Size: 1012 5316
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6328
BstBI TT'CG_AA
Cuts at: 5132 5132
Size: 6328
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6328
BstYI r'GATC_y
Cuts at: 1972 3177 3658 4025 4410 4427 5879 5965 5977
Size: 1205 481 367 385 17 1452 86 12
Cuts at: 5977 6323 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1205 481 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2400 3618 513
Size: 552 1335 1218 3223
Fragments arranged by size:
3223 1335 1218 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2553 487
Size: 683 56 1327 4262
Fragments arranged by size:
4262 1327 683 56
BtsI GCAGTG_nn'
Cuts at: 3844 4638 4658 3844
Size: 794 20 5514
Fragments arranged by size:
5514 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6328
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6293
DraI TTT'AAA
Cuts at: 3632 3632
Size: 6328
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5335 809
Size: 507 4019 1802
Fragments arranged by size:
4019 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3775 4664 6293
Size: 54 1425 50 275 18 1945 889 1629
Cuts at: 6293 8
Size: 43
Fragments arranged by size:
1945 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3775 1537
Size: 275 1963 4090
Fragments arranged by size:
4090 1963 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2656 4258 1353
Size: 1303 1602 3423
Fragments arranged by size:
3423 1602 1303
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4915 5289 5435 387
Size: 1064 138 3326 374 146 1280
Fragments arranged by size:
3326 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2565 3573 2565
Size: 1008 5320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2619 3123 3379 4391 4988 5775
Size: 393 554 433 504 256 1012 597 787
Cuts at: 5775 6043 1239
Size: 268 1524
Fragments arranged by size:
1524 1012 787 597 554 504 433 393
268 256
EcoO109I rG'GnC_Cy
Cuts at: 3530 3530
Size: 6328
EcoRI G'AATT_C
Cuts at: 2841 3429 3637 4015 2841
Size: 588 208 378 5154
Fragments arranged by size:
5154 588 378 208
FspI TGC'GCA
Cuts at: 4834 4834
Size: 6328
HaeII r_GCGC'y
Cuts at: 1678 5475 1678
Size: 3797 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2734 2766 3436 3468 4969
Size: 32 135 32 1162 32 670 32 1501
Cuts at: 4969 5001 5043 5075 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1501 1162 670 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3672 3918 669
Size: 988 281 1734 246 3079
Fragments arranged by size:
3079 1734 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2802 748
Size: 2054 4274
HpaI GTT'AAC
Cuts at: 3918 3918
Size: 6328
KpnI G_GTAC'C
Cuts at: 3653 3653
Size: 6328
MfeI C'AATT_G
Cuts at: 3927 3927
Size: 6328
MluI A'CGCG_T
Cuts at: 6204 6204
Size: 6328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5442 5626 1305
Size: 4137 184 2007
Fragments arranged by size:
4137 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4506
Fragments arranged by size:
4506 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2950 3018 3354 3454 4286 4645 4804
Size: 648 1784 68 336 100 832 359 159
Cuts at: 4804 518
Size: 2042
Fragments arranged by size:
2042 1784 832 648 359 336 159 100
68
MspA1I CmG'CkG
Cuts at: 1606 3744 4421 5569 5814 1606
Size: 2138 677 1148 245 2120
Fragments arranged by size:
2138 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2400 3618 513
Size: 552 1335 1218 3223
Fragments arranged by size:
3223 1335 1218 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6328
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6328
NotI GC'GGCC_GC
Cuts at: 3775 3775
Size: 6328
NspI r_CATG'y
Cuts at: 1132 1229 3686 5231 1132
Size: 97 2457 1545 2229
Fragments arranged by size:
2457 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6328
PciI A'CATG_T
Cuts at: 5227 5227
Size: 6328
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2401 3467 1108
Size: 1293 1066 3969
Fragments arranged by size:
3969 1293 1066
PfoI T'CCnGG_A
Cuts at: 3060 3060
Size: 6328
PmeI GTTT'AAAC
Cuts at: 3632 3632
Size: 6328
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4397 4429 5910 5942 4397
Size: 32 1481 32 4783
Fragments arranged by size:
4783 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3530 3530
Size: 6328
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6328
PsiI TTA'TAA
Cuts at: 3898 3898
Size: 6328
PstI C_TGCA'G
Cuts at: 830 3680 830
Size: 2850 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6328
SacI G_AGCT'C
Cuts at: 2567 3575 2567
Size: 1008 5320
SalI G'TCGA_C
Cuts at: 1936 3670 1936
Size: 1734 4594
SanDI GG'GwC_CC
Cuts at: 3530 3530
Size: 6328
SbfI CC_TGCA'GG
Cuts at: 3680 3680
Size: 6328
ScaI AGT'ACT
Cuts at: 4576 6288 4576
Size: 1712 4616
SfcI C'TryA_G
Cuts at: 826 1045 3676 4811 5492 5683 826
Size: 219 2631 1135 681 191 1471
Fragments arranged by size:
2631 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
SgrAI Cr'CCGG_yG
Cuts at: 1700 3017 1700
Size: 1317 5011
SmaI CCC'GGG
Cuts at: 1872 3655 1872
Size: 1783 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2143 4432 5333 5595 820
Size: 197 926 200 2289 901 262 1553
Fragments arranged by size:
2289 1553 926 901 262 200 197
SnaBI TAC'GTA
Cuts at: 493 4119 493
Size: 3626 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6328
SphI G_CATG'C
Cuts at: 1132 3686 1132
Size: 2554 3774
SspI AAT'ATT
Cuts at: 5 52 4252 5
Size: 47 4200 2081
Fragments arranged by size:
4200 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6328
StyI C'CwwG_G
Cuts at: 513 1065 2400 3618 3767 513
Size: 552 1335 1218 149 3074
Fragments arranged by size:
3074 1335 1218 552 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3095 4355 4372 4525 4710 5155 325
Size: 1255 1515 1260 17 153 185 445 1498
Fragments arranged by size:
1515 1498 1260 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2618 3502 4574
Size: 274 80 33 51 1443 641 884 1072
Cuts at: 4574 6286 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1072 884 641 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6288 6320 6288
Size: 32 6296
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6328
XbaI T'CTAG_A
Cuts at: 3664 3664
Size: 6328
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6328
XmaI C'CCGG_G
Cuts at: 1870 3653 1870
Size: 1783 4545
XmnI GAAnn'nnTTC
Cuts at: 2409 2804 2972 3140 3308 3476 4457 2409
Size: 395 168 168 168 168 981 4280
Fragments arranged by size:
4280 981 395 168 168 168 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4136 276
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI