Restriction map for
pFN21AB8773
(Circular) (Six-base) MAPSORT of: pFN21AB8773.seq Check: 4371 from: 1 to: 5428
pFN21AB8773 5428 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2433 2433
Size: 5428
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3238 278
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2771 1937
Size: 834 4594
Acc65I G'GTAC_C
Cuts at: 2749 2749
Size: 5428
AclI AA'CG_TT
Cuts at: 1563 3555 3928 1563
Size: 1992 373 3063
Fragments arranged by size:
3063 1992 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 3491 4875 5143 1632
Size: 410 1449 1384 268 1917
Fragments arranged by size:
1917 1449 1384 410 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5231
AflIII A'CryG_T
Cuts at: 1203 2121 4327 5304 1203
Size: 918 2206 977 1327
Fragments arranged by size:
2206 1327 977 918
AgeI A'CCGG_T
Cuts at: 4259 4259
Size: 5428
AhdI GACnn_n'nnGTC
Cuts at: 4157 4157
Size: 5428
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5428
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2528 2560 2528
Size: 32 5396
AlwNI CAG_nnn'CTG
Cuts at: 2384 2439 3171 4743 2384
Size: 55 732 1572 3069
Fragments arranged by size:
3069 1572 732 55
ApaI G_GGCC'C
Cuts at: 2621 2621
Size: 5428
ApaLI G'TGCA_C
Cuts at: 3485 4641 3485
Size: 1156 4272
ApoI r'AATT_y
Cuts at: 1493 2737 2963 3115 5224 1493
Size: 1244 226 152 2109 1697
Fragments arranged by size:
2109 1697 1244 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3982 160
Size: 872 2950 1606
Fragments arranged by size:
2950 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
AvaI C'yCGr_G
Cuts at: 1870 1943 2457 2633 2753 1870
Size: 73 514 176 120 4545
Fragments arranged by size:
4545 514 176 120 73
BamHI G'GATC_C
Cuts at: 2758 3125 2758
Size: 367 5061
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2251 2272 2413 2421 2606
Size: 325 217 627 464 21 141 8 185
Cuts at: 2606 2749 4204 618
Size: 143 1455 1842
Fragments arranged by size:
1842 1455 627 464 325 217 185 143
141 21 8
BanII G_rGCy'C
Cuts at: 1391 2065 2166 2621 2634 2747 1391
Size: 674 101 455 13 113 4072
Fragments arranged by size:
4072 674 455 113 101 13
BbeI G_GCGC'C
Cuts at: 2276 2276
Size: 5428
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2055 2084 928
Size: 971 156 29 4272
Fragments arranged by size:
4272 971 156 29
BbvCI CC'TCA_GC
Cuts at: 2404 2404
Size: 5428
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3619 3653 1903
Size: 34 1682 34 3678
Fragments arranged by size:
3678 1682 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3320 4530 3320
Size: 1210 4218
BclI T'GATC_A
Cuts at: 1548 2491 1548
Size: 943 4485
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4039 136
Size: 107 122 71 631 605 2367 1525
Fragments arranged by size:
2367 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5423 5423
Size: 5428
BlpI GC'TnA_GC
Cuts at: 2845 2845
Size: 5428
Bme1580I G_kGCm'C
Cuts at: 1790 2338 2621 3489 4645 1790
Size: 548 283 868 1156 2573
Fragments arranged by size:
2573 1156 868 548 283
BmgBI CAC'GTC
Cuts at: 1318 2053 2238 2554 1318
Size: 735 185 316 4192
Fragments arranged by size:
4192 735 316 185
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2390 3159 4117
Size: 416 53 83 399 24 973 769 958
Cuts at: 4117 442
Size: 1753
Fragments arranged by size:
1753 973 958 769 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5428
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2563 2563
Size: 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2531 2653 4088 1239
Size: 1292 122 1435 2579
Fragments arranged by size:
2579 1435 1292 122
Bpu10I CC'TnA_GC
Cuts at: 2404 2404
Size: 5428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3553 4418 4716 3553
Size: 865 298 4265
Fragments arranged by size:
4265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2296 2717 4091 882
Size: 617 797 421 1374 2219
Fragments arranged by size:
2219 1374 797 617 421
BsaAI yAC'GTr
Cuts at: 493 1204 2704 3219 493
Size: 711 1500 515 2702
Fragments arranged by size:
2702 1500 711 515
BsaBI GATnn'nnATC
Cuts at: 5422 5422
Size: 5428
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2273 3235 3617 4252
Size: 53 83 186 936 740 962 382 635
Cuts at: 4252 275
Size: 1451
Fragments arranged by size:
1451 962 936 740 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3861 4259 4533 4680 5091 3861
Size: 398 274 147 411 4198
Fragments arranged by size:
4198 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2291 2321 1178
Size: 30 165 30 888 30 4285
Fragments arranged by size:
4285 888 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 1186
Size: 1045 4383
BseYI C'CCAG_C
Cuts at: 2525 4631 5127 2525
Size: 2106 496 2826
Fragments arranged by size:
2826 2106 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2334 1163
Size: 573 598 4257
Fragments arranged by size:
4257 598 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2284 2878 3639 4667 1540
Size: 275 186 283 594 761 1028 2301
Fragments arranged by size:
2301 1028 761 594 283 275 186
BsiHKAI G_wGCw'C
Cuts at: 3489 3574 4645 3489
Size: 85 1071 4272
Fragments arranged by size:
4272 1071 85
BsmI GAATG_Cn'
Cuts at: 2939 3032 2939
Size: 93 5335
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2065 2166 2338 2621 2634 2747 3489
Size: 399 275 101 172 283 13 113 742
Cuts at: 3489 3574 4645 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 283 275 172 113
101 85 13
BspEI T'CCGG_A
Cuts at: 5091 5091
Size: 5428
BspHI T'CATG_A
Cuts at: 3317 5047 3317
Size: 1730 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2433 2783 844
Size: 1589 350 3489
Fragments arranged by size:
3489 1589 350
BsrBI CCG'CTC
Cuts at: 1394 1823 2178 2269 2278 3315 1394
Size: 429 355 91 9 1037 3507
Fragments arranged by size:
3507 1037 429 355 91 9
BsrDI GCAATG_nn'
Cuts at: 65 1248 2187 3923 4097 65
Size: 1183 939 1736 174 1396
Fragments arranged by size:
1736 1396 1183 939 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2069 4072 4259 1700
Size: 110 259 2003 187 2869
Fragments arranged by size:
2869 2003 259 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5428
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5428
BssSI C'ACGA_G
Cuts at: 2013 2042 3488 4500 2013
Size: 29 1446 1012 2941
Fragments arranged by size:
2941 1446 1012 29
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2342 794
Size: 1548 3880
BstBI TT'CG_AA
Cuts at: 4232 4232
Size: 5428
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5428
BstXI CCAn_nnnn'nTGG
Cuts at: 2533 2533
Size: 5428
BstYI r'GATC_y
Cuts at: 1972 2208 2301 2712 2758 3125 3510 3527 4979
Size: 236 93 411 46 367 385 17 1452
Cuts at: 4979 5065 5077 5423 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 411 385 367 346 236 93
86 46 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2295 513
Size: 552 1230 3646
Fragments arranged by size:
3646 1230 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4689
Fragments arranged by size:
4689 683 56
BtsI GCAGTG_nn'
Cuts at: 2944 3738 3758 2944
Size: 794 20 4614
Fragments arranged by size:
4614 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5393
DraI TTT'AAA
Cuts at: 2732 2732
Size: 5428
DraIII CAC_nnn'GTG
Cuts at: 2707 2707
Size: 5428
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2288 4435 809
Size: 507 972 2147 1802
Fragments arranged by size:
2147 1802 972 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2038 2067 2362
Size: 54 1425 50 275 18 208 29 295
Cuts at: 2362 2649 2875 3764 5393 8
Size: 287 226 889 1629 43
Fragments arranged by size:
1629 1425 889 295 287 275 226 208
54 50 43 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 2875 1537
Size: 275 1063 4090
Fragments arranged by size:
4090 1063 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2532 3358 1353
Size: 665 514 826 3423
Fragments arranged by size:
3423 826 665 514
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4015 4389 4535 387
Size: 1064 138 2426 374 146 1280
Fragments arranged by size:
2426 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2531 2653 3491 4088 4875 5143
Size: 393 410 489 122 838 597 787 268
Cuts at: 5143 1239
Size: 1524
Fragments arranged by size:
1524 838 787 597 489 410 393 268
122
EcoO109I rG'GnC_Cy
Cuts at: 2618 2660 2618
Size: 42 5386
EcoRI G'AATT_C
Cuts at: 2737 3115 2737
Size: 378 5050
FspI TGC'GCA
Cuts at: 3934 3934
Size: 5428
HaeII r_GCGC'y
Cuts at: 1678 2276 2439 4575 1678
Size: 598 163 2136 2531
Fragments arranged by size:
2531 2136 598 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4069 4101 4143 4175 1373
Size: 32 135 32 2497 32 42 32 2626
Fragments arranged by size:
2626 2497 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2772 3018 669
Size: 988 281 834 246 3079
Fragments arranged by size:
3079 988 834 281 246
HindIII A'AGCT_T
Cuts at: 748 2473 748
Size: 1725 3703
HpaI GTT'AAC
Cuts at: 3018 3018
Size: 5428
KasI G'GCGC_C
Cuts at: 2272 2272
Size: 5428
KpnI G_GTAC'C
Cuts at: 2753 2753
Size: 5428
MfeI C'AATT_G
Cuts at: 3027 3027
Size: 5428
MluI A'CGCG_T
Cuts at: 5304 5304
Size: 5428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2706 4542 4726 1305
Size: 1401 1836 184 2007
Fragments arranged by size:
2007 1836 1401 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2040 10
Size: 54 1425 343 208 3398
Fragments arranged by size:
3398 1425 343 208 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2156 3386 3745 3904 518
Size: 648 990 1230 359 159 2042
Fragments arranged by size:
2042 1230 990 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2104 2529 2673 2844 3521 4669 4914 1606
Size: 498 425 144 171 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 498 425 245 171 144
NaeI GCC'GGC
Cuts at: 2071 2071
Size: 5428
NarI GG'CG_CC
Cuts at: 2273 2273
Size: 5428
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4876
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5428
NgoMIV G'CCGG_C
Cuts at: 2069 2069
Size: 5428
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5428
NotI GC'GGCC_GC
Cuts at: 2875 2875
Size: 5428
NspI r_CATG'y
Cuts at: 1132 1229 2125 2155 2786 4331 1132
Size: 97 896 30 631 1545 2229
Fragments arranged by size:
2229 1545 896 631 97 30
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5428
PciI A'CATG_T
Cuts at: 2121 4327 2121
Size: 2206 3222
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2707 1108
Size: 1599 3829
PfoI T'CCnGG_A
Cuts at: 2115 2115
Size: 5428
PmeI GTTT'AAAC
Cuts at: 2732 2732
Size: 5428
PmlI CAC'GTG
Cuts at: 2704 2704
Size: 5428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 5010 5042 3497
Size: 32 1481 32 3883
Fragments arranged by size:
3883 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5428
PsiI TTA'TAA
Cuts at: 2998 2998
Size: 5428
PspOMI G'GGCC_C
Cuts at: 2617 2617
Size: 5428
PspXI vC'TCGA_Gb
Cuts at: 2633 2633
Size: 5428
PstI C_TGCA'G
Cuts at: 830 2103 2266 2430 2780 830
Size: 1273 163 164 350 3478
Fragments arranged by size:
3478 1273 350 164 163
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5428
PvuII CAG'CTG
Cuts at: 2529 2673 2529
Size: 144 5284
RsrII CG'GwC_CG
Cuts at: 2200 2200
Size: 5428
SalI G'TCGA_C
Cuts at: 1936 2770 1936
Size: 834 4594
SapI GCTCTTCn'nnn_
Cuts at: 2532 2532
Size: 5428
SbfI CC_TGCA'GG
Cuts at: 2780 2780
Size: 5428
ScaI AGT'ACT
Cuts at: 2586 3676 5388 2586
Size: 1090 1712 2626
Fragments arranged by size:
2626 1712 1090
SfcI C'TryA_G
Cuts at: 826 1045 2099 2262 2426 2776 3911 4592 4783
Size: 219 1054 163 164 350 1135 681 191
Cuts at: 4783 826
Size: 1471
Fragments arranged by size:
1471 1135 1054 681 350 219 191 164
163
SfoI GGC'GCC
Cuts at: 2274 2274
Size: 5428
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5428
SmaI CCC'GGG
Cuts at: 1872 2755 1872
Size: 883 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2457 2633 3532 4433 4695 820
Size: 197 926 514 176 899 901 262 1553
Fragments arranged by size:
1553 926 901 899 514 262 197 176
SnaBI TAC'GTA
Cuts at: 493 3219 493
Size: 2726 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5428
SphI G_CATG'C
Cuts at: 1132 2786 1132
Size: 1654 3774
SspI AAT'ATT
Cuts at: 5 52 3352 5
Size: 47 3300 2081
Fragments arranged by size:
3300 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5428
StyI C'CwwG_G
Cuts at: 513 1065 2417 2867 513
Size: 552 1352 450 3074
Fragments arranged by size:
3074 1352 552 450
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3455 3472 3625 3810 4255 325
Size: 1255 1875 17 153 185 445 1498
Fragments arranged by size:
1875 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2584 3674 5386
Size: 274 80 33 51 1443 607 1090 1712
Cuts at: 5386 96
Size: 138
Fragments arranged by size:
1712 1443 1090 607 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2222 2254 2291 2323 2359 2391 5388 5420 2222
Size: 32 37 32 36 32 2997 32 2230
Fragments arranged by size:
2997 2230 37 36 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2083 1316
Size: 767 4661
XbaI T'CTAG_A
Cuts at: 2764 2764
Size: 5428
XhoI C'TCGA_G
Cuts at: 1943 2457 2633 1943
Size: 514 176 4738
Fragments arranged by size:
4738 514 176
XmaI C'CCGG_G
Cuts at: 1870 2753 1870
Size: 883 4545
XmnI GAAnn'nnTTC
Cuts at: 3557 3557
Size: 5428
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3236 276
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PpuMI PsrI SacI SacII SanDI SexAI
SfiI SrfI SwaI XcmI