Restriction map for
pFN21AB8771
(Circular) (Six-base) MAPSORT of: pFN21AB8771.seq Check: 21 from: 1 to: 5407
pFN21AB8771 5407 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2216 2218 2344 2216
Size: 2 126 5279
Fragments arranged by size:
5279 126 2
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3217 278
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2750 1937
Size: 813 4594
Acc65I G'GTAC_C
Cuts at: 2728 2728
Size: 5407
AclI AA'CG_TT
Cuts at: 1563 3534 3907 1563
Size: 1971 373 3063
Fragments arranged by size:
3063 1971 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3470 4854 5122 1632
Size: 1838 1384 268 1917
Fragments arranged by size:
1917 1838 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5210
AflIII A'CryG_T
Cuts at: 1203 4306 5283 1203
Size: 3103 977 1327
Fragments arranged by size:
3103 1327 977
AgeI A'CCGG_T
Cuts at: 4238 4238
Size: 5407
AhdI GACnn_n'nnGTC
Cuts at: 4136 4136
Size: 5407
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5407
AlwNI CAG_nnn'CTG
Cuts at: 3150 4722 3150
Size: 1572 3835
ApaI G_GGCC'C
Cuts at: 2632 2632
Size: 5407
ApaLI G'TGCA_C
Cuts at: 3464 4620 3464
Size: 1156 4251
ApoI r'AATT_y
Cuts at: 1493 2716 2942 3094 5203 1493
Size: 1223 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 226 152
AscI GG'CGCG_CC
Cuts at: 2393 2546 2393
Size: 153 5254
AseI AT'TA_AT
Cuts at: 160 1032 3961 160
Size: 872 2929 1606
Fragments arranged by size:
2929 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
AvaI C'yCGr_G
Cuts at: 1870 1943 2180 2242 2378 2663 2732 1870
Size: 73 237 62 136 285 69 4545
Fragments arranged by size:
4545 285 237 136 73 69 62
BamHI G'GATC_C
Cuts at: 2737 3104 2737
Size: 367 5040
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2098 2131 2167 2175 2277
Size: 325 217 627 311 33 36 8 102
Cuts at: 2277 2318 2615 2728 4183 618
Size: 41 297 113 1455 1842
Fragments arranged by size:
1842 1455 627 325 311 297 217 113
102 41 36 33 8
BanII G_rGCy'C
Cuts at: 1391 2330 2632 2726 1391
Size: 939 302 94 4072
Fragments arranged by size:
4072 939 302 94
BbeI G_GCGC'C
Cuts at: 2102 2135 2171 2179 2322 2619 2102
Size: 33 36 8 143 297 4890
Fragments arranged by size:
4890 297 143 36 33 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3598 3632 1903
Size: 34 1661 34 3678
Fragments arranged by size:
3678 1661 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3299 4509 3299
Size: 1210 4197
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5407
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2613 4018 136
Size: 107 122 71 631 605 941 1405 1525
Fragments arranged by size:
1525 1405 941 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5402 5402
Size: 5407
BlpI GC'TnA_GC
Cuts at: 2824 2824
Size: 5407
Bme1580I G_kGCm'C
Cuts at: 1790 2632 3468 4624 1790
Size: 842 836 1156 2573
Fragments arranged by size:
2573 1156 842 836
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5407
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2099 2209 2398
Size: 416 53 83 399 24 682 110 189
Cuts at: 2398 3138 4096 442
Size: 740 958 1753
Fragments arranged by size:
1753 958 740 682 416 399 189 110
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2474 2516 4067 1239
Size: 1088 147 42 1551 2579
Fragments arranged by size:
2579 1551 1088 147 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2636 3532 4397 4695 2636
Size: 896 865 298 3348
Fragments arranged by size:
3348 896 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4070 882
Size: 617 2571 2219
Fragments arranged by size:
2571 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3198 493
Size: 711 1994 2702
Fragments arranged by size:
2702 1994 711
BsaBI GATnn'nnATC
Cuts at: 5401 5401
Size: 5407
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2099 2105 2111 2132
Size: 53 83 186 936 566 6 6 21
Cuts at: 2132 2168 2176 2319 2616 3214 3596 4231 275
Size: 36 8 143 297 598 382 635 1451
Fragments arranged by size:
1451 936 635 598 566 382 297 186
143 83 53 36 21 8 6 6
BsaWI w'CCGG_w
Cuts at: 3840 4238 4512 4659 5070 3840
Size: 398 274 147 411 4177
Fragments arranged by size:
4177 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5182
Fragments arranged by size:
5182 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2299 1186
Size: 837 276 4294
Fragments arranged by size:
4294 837 276
BseYI C'CCAG_C
Cuts at: 2625 4610 5106 2625
Size: 1985 496 2926
Fragments arranged by size:
2926 1985 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2011 1163
Size: 573 275 4559
Fragments arranged by size:
4559 573 275
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2122 2857 3618 4646 1540
Size: 275 186 121 735 761 1028 2301
Fragments arranged by size:
2301 1028 761 735 275 186 121
BsiHKAI G_wGCw'C
Cuts at: 2330 3468 3553 4624 2330
Size: 1138 85 1071 3113
Fragments arranged by size:
3113 1138 1071 85
BsmI GAATG_Cn'
Cuts at: 2918 3011 2918
Size: 93 5314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2330 2632 2726 3468 3553 4624 1391
Size: 399 540 302 94 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 540 399 302 94 85
BspEI T'CCGG_A
Cuts at: 5070 5070
Size: 5407
BspHI T'CATG_A
Cuts at: 3296 5026 3296
Size: 1730 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2216 2218 2344 2762 844
Size: 1372 2 126 418 3489
Fragments arranged by size:
3489 1372 418 126 2
BsrBI CCG'CTC
Cuts at: 1394 1823 2446 2458 2500 2569 2650 3294 1394
Size: 429 623 12 42 69 81 644 3507
Fragments arranged by size:
3507 644 623 429 81 69 42 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 3902 4076 65
Size: 1183 2654 174 1396
Fragments arranged by size:
2654 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2020 2095 2295 2436 4051 4238 1700
Size: 110 210 75 200 141 1615 187 2869
Fragments arranged by size:
2869 1615 210 200 187 141 110 75
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5407
BssHII G'CGCG_C
Cuts at: 1925 2289 2393 2415 2488 2530 2546 1925
Size: 364 104 22 73 42 16 4786
Fragments arranged by size:
4786 364 104 73 42 22 16
BssSI C'ACGA_G
Cuts at: 3467 4479 3467
Size: 1012 4395
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5407
BstBI TT'CG_AA
Cuts at: 4211 4211
Size: 5407
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5407
BstYI r'GATC_y
Cuts at: 1972 2737 3104 3489 3506 4958 5044 5056 5402
Size: 765 367 385 17 1452 86 12 346
Cuts at: 5402 1972
Size: 1977
Fragments arranged by size:
1977 1452 765 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2282 2282
Size: 5407
BtgI C'CryG_G
Cuts at: 513 1065 2031 2044 2236 2472 2535 513
Size: 552 966 13 192 236 63 3385
Fragments arranged by size:
3385 966 552 236 192 63 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4668
Fragments arranged by size:
4668 683 56
BtsI GCAGTG_nn'
Cuts at: 2923 3717 3737 2923
Size: 794 20 4593
Fragments arranged by size:
4593 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5372
DraI TTT'AAA
Cuts at: 2711 2711
Size: 5407
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2684 4414 809
Size: 507 1368 1730 1802
Fragments arranged by size:
1802 1730 1368 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2119 2421 2469
Size: 54 1425 50 275 18 289 302 48
Cuts at: 2469 2854 3743 5372 8
Size: 385 889 1629 43
Fragments arranged by size:
1629 1425 889 385 302 289 275 54
50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2119 2854 1537
Size: 275 307 735 4090
Fragments arranged by size:
4090 735 307 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2520 3337 1353
Size: 1167 817 3423
Fragments arranged by size:
3423 1167 817
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2521 3994 4368 4514 387
Size: 1064 138 932 1473 374 146 1280
Fragments arranged by size:
1473 1280 1064 932 374 146 138
EcoICRI GAG'CTC
Cuts at: 2328 2328
Size: 5407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2474 2516 3470 4067 4854 5122
Size: 393 695 147 42 954 597 787 268
Cuts at: 5122 1239
Size: 1524
Fragments arranged by size:
1524 954 787 695 597 393 268 147
42
EcoO109I rG'GnC_Cy
Cuts at: 2259 2629 2698 2259
Size: 370 69 4968
Fragments arranged by size:
4968 370 69
EcoRI G'AATT_C
Cuts at: 2716 3094 2716
Size: 378 5029
FspI TGC'GCA
Cuts at: 3913 3913
Size: 5407
HaeII r_GCGC'y
Cuts at: 1678 2102 2135 2171 2179 2322 2516 2579 2619
Size: 424 33 36 8 143 194 63 40
Cuts at: 2619 4554 1678
Size: 1935 2531
Fragments arranged by size:
2531 1935 424 194 143 63 40 36
33 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373
Size: 32 135 32 2476 32 42 32 2626
Fragments arranged by size:
2626 2476 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2751 2997 669
Size: 988 281 813 246 3079
Fragments arranged by size:
3079 988 813 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5407
HpaI GTT'AAC
Cuts at: 2997 2997
Size: 5407
KasI G'GCGC_C
Cuts at: 2098 2131 2167 2175 2318 2615 2098
Size: 33 36 8 143 297 4890
Fragments arranged by size:
4890 297 143 36 33 8
KpnI G_GTAC'C
Cuts at: 2732 2732
Size: 5407
MfeI C'AATT_G
Cuts at: 3006 3006
Size: 5407
MluI A'CGCG_T
Cuts at: 5283 5283
Size: 5407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4521 4705 1305
Size: 3216 184 2007
Fragments arranged by size:
3216 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3585
Fragments arranged by size:
3585 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3365 3724 3883 518
Size: 648 2199 359 159 2042
Fragments arranged by size:
2199 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2046 2238 2316 2369 2537 2636 2823 3500
Size: 440 192 78 53 168 99 187 677
Cuts at: 3500 4648 4893 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 440 245 192 187 168
99 78 53
NaeI GCC'GGC
Cuts at: 2022 2097 2438 2022
Size: 75 341 4991
Fragments arranged by size:
4991 341 75
NarI GG'CG_CC
Cuts at: 2099 2132 2168 2176 2319 2616 2099
Size: 33 36 8 143 297 4890
Fragments arranged by size:
4890 297 143 36 33 8
NcoI C'CATG_G
Cuts at: 513 1065 2031 513
Size: 552 966 3889
Fragments arranged by size:
3889 966 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5407
NgoMIV G'CCGG_C
Cuts at: 2020 2095 2436 2020
Size: 75 341 4991
Fragments arranged by size:
4991 341 75
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5407
NotI GC'GGCC_GC
Cuts at: 2854 2854
Size: 5407
NspI r_CATG'y
Cuts at: 1132 1229 2765 4310 1132
Size: 97 1536 1545 2229
Fragments arranged by size:
2229 1545 1536 97
PasI CC'CwG_GG
Cuts at: 1348 2620 1348
Size: 1272 4135
PciI A'CATG_T
Cuts at: 4306 4306
Size: 5407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5407
PfoI T'CCnGG_A
Cuts at: 2083 2083
Size: 5407
PmeI GTTT'AAAC
Cuts at: 2711 2711
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 4989 5021 3476
Size: 32 1481 32 3862
Fragments arranged by size:
3862 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2259 2698 2259
Size: 439 4968
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5407
PsiI TTA'TAA
Cuts at: 2977 2977
Size: 5407
PspOMI G'GGCC_C
Cuts at: 2628 2628
Size: 5407
PstI C_TGCA'G
Cuts at: 830 2520 2562 2583 2759 830
Size: 1690 42 21 176 3478
Fragments arranged by size:
3478 1690 176 42 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5407
SacI G_AGCT'C
Cuts at: 2330 2330
Size: 5407
SacII CC_GC'GG
Cuts at: 2047 2239 2538 2047
Size: 192 299 4916
Fragments arranged by size:
4916 299 192
SalI G'TCGA_C
Cuts at: 1936 2749 1936
Size: 813 4594
SapI GCTCTTCn'nnn_
Cuts at: 2520 2520
Size: 5407
SbfI CC_TGCA'GG
Cuts at: 2759 2759
Size: 5407
ScaI AGT'ACT
Cuts at: 3655 5367 3655
Size: 1712 3695
SfcI C'TryA_G
Cuts at: 826 1045 2516 2558 2579 2755 3890 4571 4762
Size: 219 1471 42 21 176 1135 681 191
Cuts at: 4762 826
Size: 1471
Fragments arranged by size:
1471 1471 1135 681 219 191 176 42
21
SfoI GGC'GCC
Cuts at: 2100 2133 2169 2177 2320 2617 2100
Size: 33 36 8 143 297 4890
Fragments arranged by size:
4890 297 143 36 33 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
SgrAI Cr'CCGG_yG
Cuts at: 1700 2295 1700
Size: 595 4812
SmaI CCC'GGG
Cuts at: 1872 2182 2244 2734 1872
Size: 310 62 490 4545
Fragments arranged by size:
4545 490 310 62
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2651 3511 4412 4674 820
Size: 197 926 708 860 901 262 1553
Fragments arranged by size:
1553 926 901 860 708 262 197
SnaBI TAC'GTA
Cuts at: 493 3198 493
Size: 2705 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5407
SphI G_CATG'C
Cuts at: 1132 2765 1132
Size: 1633 3774
SrfI GCCC'GGGC
Cuts at: 2244 2244
Size: 5407
SspI AAT'ATT
Cuts at: 5 52 3331 5
Size: 47 3279 2081
Fragments arranged by size:
3279 2081 47
StuI AGG'CCT
Cuts at: 1517 2147 1517
Size: 630 4777
StyI C'CwwG_G
Cuts at: 513 1065 2031 2846 513
Size: 552 966 815 3074
Fragments arranged by size:
3074 966 815 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3434 3451 3604 3789 4234 325
Size: 1255 1854 17 153 185 445 1498
Fragments arranged by size:
1854 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3653 5365 96
Size: 274 80 33 51 1443 1676 1712 138
Fragments arranged by size:
1712 1676 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5367 5399 5367
Size: 32 5375
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5407
XbaI T'CTAG_A
Cuts at: 2743 2743
Size: 5407
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5407
XmaI C'CCGG_G
Cuts at: 1870 2180 2242 2732 1870
Size: 310 62 490 4545
Fragments arranged by size:
4545 490 310 62
XmnI GAAnn'nnTTC
Cuts at: 3536 3536
Size: 5407
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3215 276
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SanDI SexAI SfiI SwaI XcmI