Restriction map for
pFN21AB8770
(Circular) (Six-base) MAPSORT of: pFN21AB8770.seq Check: 2847 from: 1 to: 5407
pFN21AB8770 5407 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3217 278
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2750 1937
Size: 813 4594
Acc65I G'GTAC_C
Cuts at: 2728 2728
Size: 5407
AclI AA'CG_TT
Cuts at: 1563 3534 3907 1563
Size: 1971 373 3063
Fragments arranged by size:
3063 1971 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3470 4854 5122 1632
Size: 1838 1384 268 1917
Fragments arranged by size:
1917 1838 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2447 820
Size: 197 1430 3780
Fragments arranged by size:
3780 1430 197
AflIII A'CryG_T
Cuts at: 1203 2697 4306 5283 1203
Size: 1494 1609 977 1327
Fragments arranged by size:
1609 1494 1327 977
AgeI A'CCGG_T
Cuts at: 4238 4238
Size: 5407
AhdI GACnn_n'nnGTC
Cuts at: 4136 4136
Size: 5407
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5407
AlwNI CAG_nnn'CTG
Cuts at: 3150 4722 3150
Size: 1572 3835
ApaLI G'TGCA_C
Cuts at: 2257 3464 4620 2257
Size: 1207 1156 3044
Fragments arranged by size:
3044 1207 1156
ApoI r'AATT_y
Cuts at: 1493 2276 2716 2942 3094 5203 1493
Size: 783 440 226 152 2109 1697
Fragments arranged by size:
2109 1697 783 440 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3961 160
Size: 872 2929 1606
Fragments arranged by size:
2929 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
AvaI C'yCGr_G
Cuts at: 1870 1943 2165 2588 2732 1870
Size: 73 222 423 144 4545
Fragments arranged by size:
4545 423 222 144 73
BamHI G'GATC_C
Cuts at: 2737 3104 2737
Size: 367 5040
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2728 4183 618
Size: 325 217 627 941 1455 1842
Fragments arranged by size:
1842 1455 941 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2663 2726 1391
Size: 1272 63 4072
Fragments arranged by size:
4072 1272 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2633 928
Size: 971 734 3702
Fragments arranged by size:
3702 971 734
BbvCI CC'TCA_GC
Cuts at: 2681 2681
Size: 5407
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3598 3632 1903
Size: 34 1661 34 3678
Fragments arranged by size:
3678 1661 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3299 4509 3299
Size: 1210 4197
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5407
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4018 136
Size: 107 122 71 631 605 2346 1525
Fragments arranged by size:
2346 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2010 5402 2010
Size: 3392 2015
BlpI GC'TnA_GC
Cuts at: 2824 2824
Size: 5407
Bme1580I G_kGCm'C
Cuts at: 1790 2261 3468 4624 1790
Size: 471 1207 1156 2573
Fragments arranged by size:
2573 1207 1156 471
BmgBI CAC'GTC
Cuts at: 1318 2700 1318
Size: 1382 4025
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3138 4096 442
Size: 416 53 83 399 24 1721 958 1753
Fragments arranged by size:
1753 1721 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5407
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 5407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2657 4067 1239
Size: 1418 1410 2579
Fragments arranged by size:
2579 1418 1410
Bpu10I CC'TnA_GC
Cuts at: 2052 2681 2052
Size: 629 4778
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2231 2306 3532 4397 4695 2231
Size: 75 1226 865 298 2943
Fragments arranged by size:
2943 1226 865 298 75
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2116 2531 4070 882
Size: 617 617 415 1539 2219
Fragments arranged by size:
2219 1539 617 617 415
BsaAI yAC'GTr
Cuts at: 493 1204 3198 493
Size: 711 1994 2702
Fragments arranged by size:
2702 1994 711
BsaBI GATnn'nnATC
Cuts at: 5401 5401
Size: 5407
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3214 3596 4231 275
Size: 53 83 186 936 1681 382 635 1451
Fragments arranged by size:
1681 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3840 4238 4512 4659 5070 3840
Size: 398 274 147 411 4177
Fragments arranged by size:
4177 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2569 2599 1178
Size: 30 165 30 1166 30 3986
Fragments arranged by size:
3986 1166 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2246 1186
Size: 1060 4347
BseYI C'CCAG_C
Cuts at: 4610 5106 4610
Size: 496 4911
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2440 2653 2711 1163
Size: 573 704 213 58 3859
Fragments arranged by size:
3859 704 573 213 58
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2857 3618 4646 1540
Size: 275 186 856 761 1028 2301
Fragments arranged by size:
2301 1028 856 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2061 2261 2523 2663 3468 3553 4624 2061
Size: 200 262 140 805 85 1071 2844
Fragments arranged by size:
2844 1071 805 262 200 140 85
BsmI GAATG_Cn'
Cuts at: 2918 3011 2918
Size: 93 5314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2261 2523 2663 2726 3468 3553
Size: 399 271 200 262 140 63 742 85
Cuts at: 3553 4624 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 271 262 200 140
85 63
BspEI T'CCGG_A
Cuts at: 5070 5070
Size: 5407
BspHI T'CATG_A
Cuts at: 3296 5026 3296
Size: 1730 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2762 844
Size: 1918 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3294 1394
Size: 429 1471 3507
Fragments arranged by size:
3507 1471 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3902 4076 65
Size: 1183 2654 174 1396
Fragments arranged by size:
2654 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2294 4051 4238 1700
Size: 110 484 1757 187 2869
Fragments arranged by size:
2869 1757 484 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5407
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5407
BssSI C'ACGA_G
Cuts at: 3467 4479 3467
Size: 1012 4395
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5407
BstBI TT'CG_AA
Cuts at: 4211 4211
Size: 5407
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5407
BstYI r'GATC_y
Cuts at: 1972 2010 2737 3104 3489 3506 4958 5044 5056
Size: 38 727 367 385 17 1452 86 12
Cuts at: 5056 5402 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 727 385 367 346 86 38
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2571 513
Size: 552 1506 3349
Fragments arranged by size:
3349 1506 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4668
Fragments arranged by size:
4668 683 56
BtsI GCAGTG_nn'
Cuts at: 2565 2923 3717 3737 2565
Size: 358 794 20 4235
Fragments arranged by size:
4235 794 358 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2452 2487 529
Size: 35 1888 35 3449
Fragments arranged by size:
3449 1888 35 35
DraI TTT'AAA
Cuts at: 2711 2711
Size: 5407
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4414 809
Size: 507 3098 1802
Fragments arranged by size:
3098 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2574 2854 3743
Size: 54 1425 50 275 18 744 280 889
Cuts at: 3743 5372 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 744 280 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2854 1537
Size: 275 1042 4090
Fragments arranged by size:
4090 1042 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3337 1353
Size: 1984 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3994 4368 4514 387
Size: 1064 138 2405 374 146 1280
Fragments arranged by size:
2405 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2661 2661
Size: 5407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2657 3470 4067 4854 5122 1239
Size: 393 1025 813 597 787 268 1524
Fragments arranged by size:
1524 1025 813 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2066 2066
Size: 5407
EcoO109I rG'GnC_Cy
Cuts at: 2602 2602
Size: 5407
EcoRI G'AATT_C
Cuts at: 2716 3094 2716
Size: 378 5029
FspI TGC'GCA
Cuts at: 3913 3913
Size: 5407
HaeII r_GCGC'y
Cuts at: 1678 4554 1678
Size: 2876 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373
Size: 32 135 32 2476 32 42 32 2626
Fragments arranged by size:
2626 2476 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2751 2997 669
Size: 988 281 813 246 3079
Fragments arranged by size:
3079 988 813 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5407
HpaI GTT'AAC
Cuts at: 2997 2997
Size: 5407
KpnI G_GTAC'C
Cuts at: 2732 2732
Size: 5407
MfeI C'AATT_G
Cuts at: 2264 3006 2264
Size: 742 4665
MluI A'CGCG_T
Cuts at: 5283 5283
Size: 5407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2327 4521 4705 1305
Size: 1022 2194 184 2007
Fragments arranged by size:
2194 2007 1022 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2576 10
Size: 54 1425 343 744 2841
Fragments arranged by size:
2841 1425 744 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3365 3724 3883 518
Size: 648 2199 359 159 2042
Fragments arranged by size:
2199 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2402 2823 3500 4648 4893 1606
Size: 796 421 677 1148 245 2120
Fragments arranged by size:
2120 1148 796 677 421 245
NaeI GCC'GGC
Cuts at: 2296 2296
Size: 5407
NcoI C'CATG_G
Cuts at: 513 1065 2571 513
Size: 552 1506 3349
Fragments arranged by size:
3349 1506 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5407
NgoMIV G'CCGG_C
Cuts at: 2294 2294
Size: 5407
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5407
NotI GC'GGCC_GC
Cuts at: 2854 2854
Size: 5407
NspI r_CATG'y
Cuts at: 1132 1229 2765 4310 1132
Size: 97 1536 1545 2229
Fragments arranged by size:
2229 1545 1536 97
PasI CC'CwG_GG
Cuts at: 1348 2514 1348
Size: 1166 4241
PciI A'CATG_T
Cuts at: 4306 4306
Size: 5407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2572 1108
Size: 1464 3943
PmeI GTTT'AAAC
Cuts at: 2711 2711
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 4989 5021 3476
Size: 32 1481 32 3862
Fragments arranged by size:
3862 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2602 2602
Size: 5407
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5407
PsiI TTA'TAA
Cuts at: 2977 2977
Size: 5407
PstI C_TGCA'G
Cuts at: 830 2210 2759 830
Size: 1380 549 3478
Fragments arranged by size:
3478 1380 549
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5407
PvuII CAG'CTG
Cuts at: 2402 2402
Size: 5407
SacI G_AGCT'C
Cuts at: 2663 2663
Size: 5407
SalI G'TCGA_C
Cuts at: 1936 2749 1936
Size: 813 4594
SbfI CC_TGCA'GG
Cuts at: 2759 2759
Size: 5407
ScaI AGT'ACT
Cuts at: 3655 5367 3655
Size: 1712 3695
SfcI C'TryA_G
Cuts at: 826 1045 2014 2206 2383 2755 3890 4571 4762
Size: 219 969 192 177 372 1135 681 191
Cuts at: 4762 826
Size: 1471
Fragments arranged by size:
1471 1135 969 681 372 219 192 191
177
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5407
SmaI CCC'GGG
Cuts at: 1872 2167 2734 1872
Size: 295 567 4545
Fragments arranged by size:
4545 567 295
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2246 2321 2447 3511 4412 4674
Size: 197 926 303 75 126 1064 901 262
Cuts at: 4674 820
Size: 1553
Fragments arranged by size:
1553 1064 926 901 303 262 197 126
75
SnaBI TAC'GTA
Cuts at: 493 3198 493
Size: 2705 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5407
SphI G_CATG'C
Cuts at: 1132 2765 1132
Size: 1633 3774
SspI AAT'ATT
Cuts at: 5 52 3331 5
Size: 47 3279 2081
Fragments arranged by size:
3279 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5407
StyI C'CwwG_G
Cuts at: 513 1065 2571 2846 513
Size: 552 1506 275 3074
Fragments arranged by size:
3074 1506 552 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2156 3434 3451 3604 3789 4234 325
Size: 1255 576 1278 17 153 185 445 1498
Fragments arranged by size:
1498 1278 1255 576 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3653 5365 96
Size: 274 80 33 51 1443 1676 1712 138
Fragments arranged by size:
1712 1676 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2569 2601 5367 5399 2569
Size: 32 2766 32 2577
Fragments arranged by size:
2766 2577 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5407
XbaI T'CTAG_A
Cuts at: 2743 2743
Size: 5407
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5407
XmaI C'CCGG_G
Cuts at: 1870 2165 2732 1870
Size: 295 567 4545
Fragments arranged by size:
4545 567 295
XmnI GAAnn'nnTTC
Cuts at: 3536 3536
Size: 5407
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3215 276
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI