Restriction map for
pFN21AB8767
(Circular) (Six-base) MAPSORT of: pFN21AB8767.seq Check: 7954 from: 1 to: 5380
pFN21AB8767 5380 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3190 278
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2723 1937
Size: 786 4594
Acc65I G'GTAC_C
Cuts at: 2701 2701
Size: 5380
AclI AA'CG_TT
Cuts at: 1563 3507 3880 1563
Size: 1944 373 3063
Fragments arranged by size:
3063 1944 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2062 2555 3443 4827 5095 1632
Size: 430 493 888 1384 268 1917
Fragments arranged by size:
1917 1384 888 493 430 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5183
AflIII A'CryG_T
Cuts at: 1203 4279 5256 1203
Size: 3076 977 1327
Fragments arranged by size:
3076 1327 977
AgeI A'CCGG_T
Cuts at: 4211 4211
Size: 5380
AhdI GACnn_n'nnGTC
Cuts at: 4109 4109
Size: 5380
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2475 1184
Size: 1291 4089
AlwNI CAG_nnn'CTG
Cuts at: 3123 4695 3123
Size: 1572 3808
ApaI G_GGCC'C
Cuts at: 2643 2643
Size: 5380
ApaLI G'TGCA_C
Cuts at: 2375 3437 4593 2375
Size: 1062 1156 3162
Fragments arranged by size:
3162 1156 1062
ApoI r'AATT_y
Cuts at: 1493 2689 2915 3067 5176 1493
Size: 1196 226 152 2109 1697
Fragments arranged by size:
2109 1697 1196 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3934 160
Size: 872 2902 1606
Fragments arranged by size:
2902 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
AvaI C'yCGr_G
Cuts at: 1870 1943 2447 2705 1870
Size: 73 504 258 4545
Fragments arranged by size:
4545 504 258 73
BamHI G'GATC_C
Cuts at: 2710 3077 2710
Size: 367 5013
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2201 2443 2701 4156 618
Size: 325 217 627 414 242 258 1455 1842
Fragments arranged by size:
1842 1455 627 414 325 258 242 217
BanII G_rGCy'C
Cuts at: 1391 2196 2404 2643 2699 1391
Size: 805 208 239 56 4072
Fragments arranged by size:
4072 805 239 208 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2594 928
Size: 971 695 3714
Fragments arranged by size:
3714 971 695
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2161 2195 2564 2598 3571 3605 1903
Size: 34 224 34 369 34 973 34 3678
Fragments arranged by size:
3678 973 369 224 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3272 4482 3272
Size: 1210 4170
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5380
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3991 136
Size: 107 122 71 631 605 2319 1525
Fragments arranged by size:
2319 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5375 5375
Size: 5380
BlpI GC'TnA_GC
Cuts at: 2249 2797 2249
Size: 548 4832
Bme1580I G_kGCm'C
Cuts at: 1790 2349 2379 2448 2643 3441 4597 1790
Size: 559 30 69 195 798 1156 2573
Fragments arranged by size:
2573 1156 798 559 195 69 30
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5380
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2257 3111 4069
Size: 416 53 83 399 24 840 854 958
Cuts at: 4069 442
Size: 1753
Fragments arranged by size:
1753 958 854 840 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2167 2418 4040 1239
Size: 928 251 1622 2579
Fragments arranged by size:
2579 1622 928 251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 2351 3505 4370 4668 2054
Size: 297 1154 865 298 2766
Fragments arranged by size:
2766 1154 865 298 297
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4043 882
Size: 617 2544 2219
Fragments arranged by size:
2544 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3171 493
Size: 711 1967 2702
Fragments arranged by size:
2702 1967 711
BsaBI GATnn'nnATC
Cuts at: 5374 5374
Size: 5380
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3187 3569 4204 275
Size: 53 83 186 936 1654 382 635 1451
Fragments arranged by size:
1654 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3813 4211 4485 4632 5043 3813
Size: 398 274 147 411 4150
Fragments arranged by size:
4150 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2492 2522 2594 2624 1178
Size: 30 165 30 1089 30 72 30 3934
Fragments arranged by size:
3934 1089 165 72 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2425 2542 1186
Size: 1239 117 4024
Fragments arranged by size:
4024 1239 117
BseYI C'CCAG_C
Cuts at: 2463 2628 4583 5079 2463
Size: 165 1955 496 2764
Fragments arranged by size:
2764 1955 496 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4807
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2830 3591 4619 1540
Size: 275 186 829 761 1028 2301
Fragments arranged by size:
2301 1028 829 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2336 2367 2379 3441 3526 4597 2336
Size: 31 12 1062 85 1071 3119
Fragments arranged by size:
3119 1071 1062 85 31 12
BsmI GAATG_Cn'
Cuts at: 2891 2984 2891
Size: 93 5287
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2336 2349 2367 2379 2404 2448
Size: 399 406 140 13 18 12 25 44
Cuts at: 2448 2643 2699 3441 3526 4597 1391
Size: 195 56 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 406 399 195 140 85
56 44 25 18 13 12
BspEI T'CCGG_A
Cuts at: 5043 5043
Size: 5380
BspHI T'CATG_A
Cuts at: 3269 4999 3269
Size: 1730 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2184 2289 2735 844
Size: 1340 105 446 3489
Fragments arranged by size:
3489 1340 446 105
BsrBI CCG'CTC
Cuts at: 1394 1823 2136 3267 1394
Size: 429 313 1131 3507
Fragments arranged by size:
3507 1131 429 313
BsrDI GCAATG_nn'
Cuts at: 65 1248 3875 4049 65
Size: 1183 2627 174 1396
Fragments arranged by size:
2627 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2159 2198 2541 4024 4211 1700
Size: 110 349 39 343 1483 187 2869
Fragments arranged by size:
2869 1483 349 343 187 110 39
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5380
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5380
BssSI C'ACGA_G
Cuts at: 3440 4452 3440
Size: 1012 4368
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2344 794
Size: 1550 3830
BstBI TT'CG_AA
Cuts at: 4184 4184
Size: 5380
BstEII G'GTnAC_C
Cuts at: 1184 2008 1184
Size: 824 4556
BstYI r'GATC_y
Cuts at: 1972 2523 2710 3077 3462 3479 4931 5017 5029
Size: 551 187 367 385 17 1452 86 12
Cuts at: 5029 5375 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 551 385 367 346 187 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3889
Fragments arranged by size:
3889 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4641
Fragments arranged by size:
4641 683 56
BtsI GCAGTG_nn'
Cuts at: 2121 2160 2896 3690 3710 2121
Size: 39 736 794 20 3791
Fragments arranged by size:
3791 794 736 39 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5345
DraI TTT'AAA
Cuts at: 2684 2684
Size: 5380
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4387 809
Size: 507 3071 1802
Fragments arranged by size:
3071 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2562 2599 2827
Size: 54 1425 50 275 18 732 37 228
Cuts at: 2827 3716 5345 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 732 275 228 54 50
43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 2827 1537
Size: 275 1015 4090
Fragments arranged by size:
4090 1015 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2485 2574 3310 1353
Size: 1132 89 736 3423
Fragments arranged by size:
3423 1132 736 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3967 4341 4487 387
Size: 1064 138 2378 374 146 1280
Fragments arranged by size:
2378 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2167 2418 2555 3443 4040 4827
Size: 393 430 105 251 137 888 597 787
Cuts at: 4827 5095 1239
Size: 268 1524
Fragments arranged by size:
1524 888 787 597 430 393 268 251
137 105
EcoO109I rG'GnC_Cy
Cuts at: 2639 2639
Size: 5380
EcoRI G'AATT_C
Cuts at: 2689 3067 2689
Size: 378 5002
FspI TGC'GCA
Cuts at: 2120 2285 3886 2120
Size: 165 1601 3614
Fragments arranged by size:
3614 1601 165
HaeII r_GCGC'y
Cuts at: 1678 2236 4527 1678
Size: 558 2291 2531
Fragments arranged by size:
2531 2291 558
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2441 2473 4021 4053 4095
Size: 32 135 32 869 32 1548 32 42
Cuts at: 4095 4127 1373
Size: 32 2626
Fragments arranged by size:
2626 1548 869 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2596 2724 2970 669
Size: 988 281 658 128 246 3079
Fragments arranged by size:
3079 988 658 281 246 128
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5380
HpaI GTT'AAC
Cuts at: 2970 2970
Size: 5380
KpnI G_GTAC'C
Cuts at: 2705 2705
Size: 5380
MfeI C'AATT_G
Cuts at: 2979 2979
Size: 5380
MluI A'CGCG_T
Cuts at: 5256 5256
Size: 5380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2564 2580 4494 4678 1305
Size: 1259 16 1914 184 2007
Fragments arranged by size:
2007 1914 1259 184 16
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2564 10
Size: 54 1425 343 732 2826
Fragments arranged by size:
2826 1425 732 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3338 3697 3856 518
Size: 648 2172 359 159 2042
Fragments arranged by size:
2172 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2101 2632 2796 3473 4621 4866 1606
Size: 495 531 164 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 531 495 245 164
NaeI GCC'GGC
Cuts at: 2200 2543 2200
Size: 343 5037
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3889
Fragments arranged by size:
3889 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5380
NgoMIV G'CCGG_C
Cuts at: 2198 2541 2198
Size: 343 5037
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5380
NotI GC'GGCC_GC
Cuts at: 2827 2827
Size: 5380
NspI r_CATG'y
Cuts at: 1132 1229 2389 2738 4283 1132
Size: 97 1160 349 1545 2229
Fragments arranged by size:
2229 1545 1160 349 97
PasI CC'CwG_GG
Cuts at: 1348 2474 1348
Size: 1126 4254
PciI A'CATG_T
Cuts at: 4279 4279
Size: 5380
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2104 2474 1108
Size: 996 370 4014
Fragments arranged by size:
4014 996 370
PfoI T'CCnGG_A
Cuts at: 2655 2655
Size: 5380
PmeI GTTT'AAAC
Cuts at: 2684 2684
Size: 5380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 4962 4994 3449
Size: 32 1481 32 3835
Fragments arranged by size:
3835 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5380
PsiI TTA'TAA
Cuts at: 2950 2950
Size: 5380
PspOMI G'GGCC_C
Cuts at: 2639 2639
Size: 5380
PstI C_TGCA'G
Cuts at: 830 2130 2319 2732 830
Size: 1300 189 413 3478
Fragments arranged by size:
3478 1300 413 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5380
PvuII CAG'CTG
Cuts at: 2101 2632 2101
Size: 531 4849
SalI G'TCGA_C
Cuts at: 1936 2722 1936
Size: 786 4594
SapI GCTCTTCn'nnn_
Cuts at: 2485 2485
Size: 5380
SbfI CC_TGCA'GG
Cuts at: 2732 2732
Size: 5380
ScaI AGT'ACT
Cuts at: 3628 5340 3628
Size: 1712 3668
SfcI C'TryA_G
Cuts at: 826 1045 2126 2315 2728 3863 4544 4735 826
Size: 219 1081 189 413 1135 681 191 1471
Fragments arranged by size:
1471 1135 1081 681 413 219 191 189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5380
SmaI CCC'GGG
Cuts at: 1872 2707 1872
Size: 835 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2069 2366 3484 4385 4647 820
Size: 197 926 126 297 1118 901 262 1553
Fragments arranged by size:
1553 1118 926 901 297 262 197 126
SnaBI TAC'GTA
Cuts at: 493 3171 493
Size: 2678 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5380
SphI G_CATG'C
Cuts at: 1132 2738 1132
Size: 1606 3774
SspI AAT'ATT
Cuts at: 5 52 3304 5
Size: 47 3252 2081
Fragments arranged by size:
3252 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5380
StyI C'CwwG_G
Cuts at: 513 1065 2004 2819 513
Size: 552 939 815 3074
Fragments arranged by size:
3074 939 815 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2027 2642 3407 3424 3577 3762 4207
Size: 1255 447 615 765 17 153 185 445
Cuts at: 4207 325
Size: 1498
Fragments arranged by size:
1498 1255 765 615 447 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3626 5338 96
Size: 274 80 33 51 1443 1649 1712 138
Fragments arranged by size:
1712 1649 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2594 2626 5340 5372 2594
Size: 32 2714 32 2602
Fragments arranged by size:
2714 2602 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2263 1316
Size: 947 4433
XbaI T'CTAG_A
Cuts at: 2716 2716
Size: 5380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2471 2471
Size: 5380
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5380
XmaI C'CCGG_G
Cuts at: 1870 2705 1870
Size: 835 4545
XmnI GAAnn'nnTTC
Cuts at: 3509 3509
Size: 5380
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3188 276
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI