Restriction map for
pFN21AB8766
(Circular) (Six-base) MAPSORT of: pFN21AB8766.seq Check: 7908 from: 1 to: 5380
pFN21AB8766 5380 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2299 2299
Size: 5380
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3190 278
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2009 2723 1937
Size: 72 714 4594
Fragments arranged by size:
4594 714 72
Acc65I G'GTAC_C
Cuts at: 2060 2701 2060
Size: 641 4739
AclI AA'CG_TT
Cuts at: 1563 3507 3880 1563
Size: 1944 373 3063
Fragments arranged by size:
3063 1944 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2247 3443 4827 5095 1632
Size: 615 1196 1384 268 1917
Fragments arranged by size:
1917 1384 1196 615 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5183
AflIII A'CryG_T
Cuts at: 1203 4279 5256 1203
Size: 3076 977 1327
Fragments arranged by size:
3076 1327 977
AgeI A'CCGG_T
Cuts at: 4211 4211
Size: 5380
AhdI GACnn_n'nnGTC
Cuts at: 4109 4109
Size: 5380
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5380
AlwNI CAG_nnn'CTG
Cuts at: 3123 4695 3123
Size: 1572 3808
ApaI G_GGCC'C
Cuts at: 2523 2523
Size: 5380
ApaLI G'TGCA_C
Cuts at: 3437 4593 3437
Size: 1156 4224
ApoI r'AATT_y
Cuts at: 1493 2639 2689 2915 3067 5176 1493
Size: 1146 50 226 152 2109 1697
Fragments arranged by size:
2109 1697 1146 226 152 50
AseI AT'TA_AT
Cuts at: 160 1032 3934 160
Size: 872 2902 1606
Fragments arranged by size:
2902 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
AvaI C'yCGr_G
Cuts at: 1870 1943 2705 1870
Size: 73 762 4545
Fragments arranged by size:
4545 762 73
BamHI G'GATC_C
Cuts at: 2710 3077 2710
Size: 367 5013
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2701 4156 618
Size: 325 217 627 273 641 1455 1842
Fragments arranged by size:
1842 1455 641 627 325 273 217
BanII G_rGCy'C
Cuts at: 1391 2084 2367 2523 2699 1391
Size: 693 283 156 176 4072
Fragments arranged by size:
4072 693 283 176 156
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2297 2331 3571 3605 1903
Size: 34 360 34 1240 34 3678
Fragments arranged by size:
3678 1240 360 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3272 4482 3272
Size: 1210 4170
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5380
BfrBI ATG'CAT
Cuts at: 2453 2453
Size: 5380
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3991 136
Size: 107 122 71 631 605 2319 1525
Fragments arranged by size:
2319 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2295 5375 2295
Size: 3080 2300
BlpI GC'TnA_GC
Cuts at: 2110 2797 2110
Size: 687 4693
Bme1580I G_kGCm'C
Cuts at: 1790 2211 2523 3441 4597 1790
Size: 421 312 918 1156 2573
Fragments arranged by size:
2573 1156 918 421 312
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5380
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2208 3111 4069
Size: 416 53 83 399 24 791 903 958
Cuts at: 4069 442
Size: 1753
Fragments arranged by size:
1753 958 903 791 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5380
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 5380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2381 2585 4040 1239
Size: 1061 81 204 1455 2579
Fragments arranged by size:
2579 1455 1061 204 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3505 4370 4668 3505
Size: 865 298 4217
Fragments arranged by size:
4217 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4043 882
Size: 617 2544 2219
Fragments arranged by size:
2544 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2384 3171 493
Size: 711 1180 787 2702
Fragments arranged by size:
2702 1180 787 711
BsaBI GATnn'nnATC
Cuts at: 5374 5374
Size: 5380
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3187 3569 4204 275
Size: 53 83 186 936 1654 382 635 1451
Fragments arranged by size:
1654 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3813 4211 4485 4632 5043 3813
Size: 398 274 147 411 4150
Fragments arranged by size:
4150 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5155
Fragments arranged by size:
5155 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2456 1186
Size: 1270 4110
BseYI C'CCAG_C
Cuts at: 2159 4583 5079 2159
Size: 2424 496 2460
Fragments arranged by size:
2460 2424 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2406 1163
Size: 573 670 4137
Fragments arranged by size:
4137 670 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2155 2830 3591 4619 1540
Size: 275 186 154 675 761 1028 2301
Fragments arranged by size:
2301 1028 761 675 275 186 154
BsiHKAI G_wGCw'C
Cuts at: 2367 3441 3526 4597 2367
Size: 1074 85 1071 3150
Fragments arranged by size:
3150 1074 1071 85
BsmI GAATG_Cn'
Cuts at: 2891 2984 2891
Size: 93 5287
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2211 2367 2523 2699 3441 3526
Size: 399 294 127 156 156 176 742 85
Cuts at: 3526 4597 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 294 176 156 156
127 85
BspEI T'CCGG_A
Cuts at: 5043 5043
Size: 5380
BspHI T'CATG_A
Cuts at: 3269 4999 3269
Size: 1730 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2199 2299 2735 844
Size: 1355 100 436 3489
Fragments arranged by size:
3489 1355 436 100
BsrBI CCG'CTC
Cuts at: 1394 1823 3267 1394
Size: 429 1444 3507
Fragments arranged by size:
3507 1444 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3875 4049 65
Size: 1183 2627 174 1396
Fragments arranged by size:
2627 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4024 4211 1700
Size: 110 2214 187 2869
Fragments arranged by size:
2869 2214 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5380
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5380
BssSI C'ACGA_G
Cuts at: 3440 4452 3440
Size: 1012 4368
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2329 794
Size: 1535 3845
BstBI TT'CG_AA
Cuts at: 4184 4184
Size: 5380
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5380
BstXI CCAn_nnnn'nTGG
Cuts at: 2056 2056
Size: 5380
BstYI r'GATC_y
Cuts at: 1972 2295 2710 3077 3462 3479 4931 5017 5029
Size: 323 415 367 385 17 1452 86 12
Cuts at: 5029 5375 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 415 385 367 346 323 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2066 2066
Size: 5380
BtgI C'CryG_G
Cuts at: 513 1065 2124 513
Size: 552 1059 3769
Fragments arranged by size:
3769 1059 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4641
Fragments arranged by size:
4641 683 56
BtsI GCAGTG_nn'
Cuts at: 2557 2896 3690 3710 2557
Size: 339 794 20 4227
Fragments arranged by size:
4227 794 339 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5345
DraI TTT'AAA
Cuts at: 2684 2684
Size: 5380
DraIII CAC_nnn'GTG
Cuts at: 2676 2676
Size: 5380
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4387 809
Size: 507 3071 1802
Fragments arranged by size:
3071 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2827 3716 5345
Size: 54 1425 50 275 18 997 889 1629
Cuts at: 5345 8
Size: 43
Fragments arranged by size:
1629 1425 997 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2827 1537
Size: 275 1015 4090
Fragments arranged by size:
4090 1015 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3310 1353
Size: 1957 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3967 4341 4487 387
Size: 1064 138 2378 374 146 1280
Fragments arranged by size:
2378 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2365 2365
Size: 5380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2247 2300 2381 2585 3443 4040 4827
Size: 393 615 53 81 204 858 597 787
Cuts at: 4827 5095 1239
Size: 268 1524
Fragments arranged by size:
1524 858 787 615 597 393 268 204
81 53
EcoO109I rG'GnC_Cy
Cuts at: 2467 2520 2467
Size: 53 5327
EcoRI G'AATT_C
Cuts at: 2639 2689 3067 2639
Size: 50 378 4952
Fragments arranged by size:
4952 378 50
FspI TGC'GCA
Cuts at: 2195 3886 2195
Size: 1691 3689
HaeII r_GCGC'y
Cuts at: 1678 4527 1678
Size: 2849 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2297 2329 4021 4053 4095
Size: 32 135 32 725 32 1692 32 42
Cuts at: 4095 4127 1373
Size: 32 2626
Fragments arranged by size:
2626 1692 725 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2724 2970 669
Size: 988 281 786 246 3079
Fragments arranged by size:
3079 988 786 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5380
HpaI GTT'AAC
Cuts at: 2970 2970
Size: 5380
KpnI G_GTAC'C
Cuts at: 2064 2705 2064
Size: 641 4739
MfeI C'AATT_G
Cuts at: 2979 2979
Size: 5380
MluI A'CGCG_T
Cuts at: 5256 5256
Size: 5380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4494 4678 1305
Size: 3189 184 2007
Fragments arranged by size:
3189 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3558
Fragments arranged by size:
3558 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3338 3697 3856 518
Size: 648 2172 359 159 2042
Fragments arranged by size:
2172 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2796 3473 4621 4866 1606
Size: 1190 677 1148 245 2120
Fragments arranged by size:
2120 1190 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4828
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5380
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5380
NotI GC'GGCC_GC
Cuts at: 2827 2827
Size: 5380
NsiI A_TGCA'T
Cuts at: 2455 2455
Size: 5380
NspI r_CATG'y
Cuts at: 1132 1229 2290 2738 4283 1132
Size: 97 1061 448 1545 2229
Fragments arranged by size:
2229 1545 1061 448 97
PasI CC'CwG_GG
Cuts at: 1348 2056 2584 1348
Size: 708 528 4144
Fragments arranged by size:
4144 708 528
PciI A'CATG_T
Cuts at: 4279 4279
Size: 5380
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5380
PfoI T'CCnGG_A
Cuts at: 2181 2181
Size: 5380
PmeI GTTT'AAAC
Cuts at: 2684 2684
Size: 5380
PmlI CAC'GTG
Cuts at: 2384 2384
Size: 5380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2231 2263 3449 3481 4962 4994 2231
Size: 32 1186 32 1481 32 2617
Fragments arranged by size:
2617 1481 1186 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2467 2467
Size: 5380
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5380
PsiI TTA'TAA
Cuts at: 2950 2950
Size: 5380
PspOMI G'GGCC_C
Cuts at: 2519 2519
Size: 5380
PstI C_TGCA'G
Cuts at: 830 2484 2732 830
Size: 1654 248 3478
Fragments arranged by size:
3478 1654 248
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5380
SacI G_AGCT'C
Cuts at: 2367 2367
Size: 5380
SalI G'TCGA_C
Cuts at: 1936 2722 1936
Size: 786 4594
SbfI CC_TGCA'GG
Cuts at: 2732 2732
Size: 5380
ScaI AGT'ACT
Cuts at: 3628 5340 3628
Size: 1712 3668
SexAI A'CCwGG_T
Cuts at: 2672 2672
Size: 5380
SfcI C'TryA_G
Cuts at: 826 1045 2347 2480 2728 3863 4544 4735 826
Size: 219 1302 133 248 1135 681 191 1471
Fragments arranged by size:
1471 1302 1135 681 248 219 191 133
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5380
SmaI CCC'GGG
Cuts at: 1872 2707 1872
Size: 835 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3484 4385 4647 820
Size: 197 926 1541 901 262 1553
Fragments arranged by size:
1553 1541 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3171 493
Size: 2678 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5380
SphI G_CATG'C
Cuts at: 1132 2738 1132
Size: 1606 3774
SspI AAT'ATT
Cuts at: 5 52 3304 5
Size: 47 3252 2081
Fragments arranged by size:
3252 2081 47
StuI AGG'CCT
Cuts at: 1517 2120 1517
Size: 603 4777
StyI C'CwwG_G
Cuts at: 513 1065 2115 2819 513
Size: 552 1050 704 3074
Fragments arranged by size:
3074 1050 704 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3407 3424 3577 3762 4207 325
Size: 1255 1827 17 153 185 445 1498
Fragments arranged by size:
1827 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3626 5338 96
Size: 274 80 33 51 1443 1649 1712 138
Fragments arranged by size:
1712 1649 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2205 2237 5340 5372 2205
Size: 32 3103 32 2213
Fragments arranged by size:
3103 2213 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5380
XbaI T'CTAG_A
Cuts at: 2716 2716
Size: 5380
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5380
XmaI C'CCGG_G
Cuts at: 1870 2705 1870
Size: 835 4545
XmnI GAAnn'nnTTC
Cuts at: 2495 3509 2495
Size: 1014 4366
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3188 276
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI
PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI