Restriction map for
pFN21AB8765
(Circular) (Six-base) MAPSORT of: pFN21AB8765.seq Check: 6814 from: 1 to: 5374
pFN21AB8765 5374 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2377 2377
Size: 5374
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3184 278
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2187 2717 1937
Size: 250 530 4594
Fragments arranged by size:
4594 530 250
Acc65I G'GTAC_C
Cuts at: 2695 2695
Size: 5374
AclI AA'CG_TT
Cuts at: 1563 3501 3874 1563
Size: 1938 373 3063
Fragments arranged by size:
3063 1938 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3437 4821 5089 1632
Size: 1805 1384 268 1917
Fragments arranged by size:
1917 1805 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5177
AflIII A'CryG_T
Cuts at: 1203 4273 5250 1203
Size: 3070 977 1327
Fragments arranged by size:
3070 1327 977
AgeI A'CCGG_T
Cuts at: 4205 4205
Size: 5374
AhdI GACnn_n'nnGTC
Cuts at: 2058 4103 2058
Size: 2045 3329
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5374
AlwNI CAG_nnn'CTG
Cuts at: 2299 2641 3117 4689 2299
Size: 342 476 1572 2984
Fragments arranged by size:
2984 1572 476 342
ApaI G_GGCC'C
Cuts at: 2332 2332
Size: 5374
ApaLI G'TGCA_C
Cuts at: 3431 4587 3431
Size: 1156 4218
ApoI r'AATT_y
Cuts at: 1493 2099 2683 2909 3061 5170 1493
Size: 606 584 226 152 2109 1697
Fragments arranged by size:
2109 1697 606 584 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3928 160
Size: 872 2896 1606
Fragments arranged by size:
2896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 2699 1870
Size: 73 125 631 4545
Fragments arranged by size:
4545 631 125 73
BamHI G'GATC_C
Cuts at: 2704 3071 2704
Size: 367 5007
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2304 2695 4150 618
Size: 325 217 627 517 391 1455 1842
Fragments arranged by size:
1842 1455 627 517 391 325 217
BanII G_rGCy'C
Cuts at: 1391 2332 2421 2693 1391
Size: 941 89 272 4072
Fragments arranged by size:
4072 941 272 89
BbeI G_GCGC'C
Cuts at: 2308 2308
Size: 5374
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4403
BbvCI CC'TCA_GC
Cuts at: 2300 2300
Size: 5374
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3565 3599 1903
Size: 34 1628 34 3678
Fragments arranged by size:
3678 1628 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3266 4476 3266
Size: 1210 4164
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5374
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2406 3985 136
Size: 107 122 71 631 605 734 1579 1525
Fragments arranged by size:
1579 1525 734 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5369 5369
Size: 5374
BlpI GC'TnA_GC
Cuts at: 2791 2791
Size: 5374
Bme1580I G_kGCm'C
Cuts at: 1790 2332 3435 4591 1790
Size: 542 1103 1156 2573
Fragments arranged by size:
2573 1156 1103 542
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5374
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2100 3105 4063
Size: 416 53 83 399 24 683 1005 958
Cuts at: 4063 442
Size: 1753
Fragments arranged by size:
1753 1005 958 683 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2136 2187 4034 1239
Size: 897 51 1847 2579
Fragments arranged by size:
2579 1847 897 51
Bpu10I CC'TnA_GC
Cuts at: 2300 2339 2300
Size: 39 5335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2387 3499 4364 4662 2387
Size: 1112 865 298 3099
Fragments arranged by size:
3099 1112 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4037 882
Size: 617 2538 2219
Fragments arranged by size:
2538 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3165 493
Size: 711 1961 2702
Fragments arranged by size:
2702 1961 711
BsaBI GATnn'nnATC
Cuts at: 5368 5368
Size: 5374
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2305 2444 3181 3563
Size: 53 83 186 936 772 139 737 382
Cuts at: 3563 4198 275
Size: 635 1451
Fragments arranged by size:
1451 936 772 737 635 382 186 139
83 53
BsaWI w'CCGG_w
Cuts at: 3807 4205 4479 4626 5037 3807
Size: 398 274 147 411 4144
Fragments arranged by size:
4144 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5149
Fragments arranged by size:
5149 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5374
BseYI C'CCAG_C
Cuts at: 2144 2237 2438 2494 2518 2562 4577 5073 2144
Size: 93 201 56 24 44 2015 496 2445
Fragments arranged by size:
2445 2015 496 201 93 56 44 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2116 2374 1163
Size: 573 380 258 4163
Fragments arranged by size:
4163 573 380 258
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2824 3585 4613 1540
Size: 275 186 823 761 1028 2301
Fragments arranged by size:
2301 1028 823 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3435 3520 4591 3435
Size: 85 1071 4218
Fragments arranged by size:
4218 1071 85
BsmI GAATG_Cn'
Cuts at: 2885 2978 2885
Size: 93 5281
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2332 2421 2693 3435 3520 4591 1391
Size: 399 542 89 272 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 542 399 272 89 85
BspEI T'CCGG_A
Cuts at: 5037 5037
Size: 5374
BspHI T'CATG_A
Cuts at: 3263 4993 3263
Size: 1730 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2377 2729 844
Size: 1533 352 3489
Fragments arranged by size:
3489 1533 352
BsrBI CCG'CTC
Cuts at: 1394 1823 3261 1394
Size: 429 1438 3507
Fragments arranged by size:
3507 1438 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3869 4043 65
Size: 1183 2621 174 1396
Fragments arranged by size:
2621 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4018 4205 1700
Size: 110 2208 187 2869
Fragments arranged by size:
2869 2208 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5374
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5374
BssSI C'ACGA_G
Cuts at: 3434 4446 3434
Size: 1012 4362
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5374
BstBI TT'CG_AA
Cuts at: 4178 4178
Size: 5374
BstEII G'GTnAC_C
Cuts at: 1184 2032 1184
Size: 848 4526
BstXI CCAn_nnnn'nTGG
Cuts at: 2152 2152
Size: 5374
BstYI r'GATC_y
Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369
Size: 732 367 385 17 1452 86 12 346
Cuts at: 5369 1972
Size: 1977
Fragments arranged by size:
1977 1452 732 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2433 2433
Size: 5374
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4822
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4635
Fragments arranged by size:
4635 683 56
BtsI GCAGTG_nn'
Cuts at: 2890 3684 3704 2890
Size: 794 20 4560
Fragments arranged by size:
4560 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5339
DraI TTT'AAA
Cuts at: 2678 2678
Size: 5374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4381 809
Size: 507 3065 1802
Fragments arranged by size:
3065 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2821 3710 5339
Size: 54 1425 50 275 18 991 889 1629
Cuts at: 5339 8
Size: 43
Fragments arranged by size:
1629 1425 991 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2821 1537
Size: 275 1009 4090
Fragments arranged by size:
4090 1009 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2508 3304 1353
Size: 1155 796 3423
Fragments arranged by size:
3423 1155 796
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3961 4335 4481 387
Size: 1064 138 2372 374 146 1280
Fragments arranged by size:
2372 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2187 3437 4034 4821 5089 1239
Size: 393 504 51 1250 597 787 268 1524
Fragments arranged by size:
1524 1250 787 597 504 393 268 51
EcoO109I rG'GnC_Cy
Cuts at: 2065 2328 2329 2638 2065
Size: 263 1 309 4801
Fragments arranged by size:
4801 309 263 1
EcoRI G'AATT_C
Cuts at: 2099 2683 3061 2099
Size: 584 378 4412
Fragments arranged by size:
4412 584 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2195 2195
Size: 5374
FspI TGC'GCA
Cuts at: 3880 3880
Size: 5374
HaeII r_GCGC'y
Cuts at: 1678 2308 4521 1678
Size: 630 2213 2531
Fragments arranged by size:
2531 2213 630
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2044 2076 2255 2287 2434
Size: 32 135 32 472 32 179 32 147
Cuts at: 2434 2466 4015 4047 4089 4121 1373
Size: 32 1549 32 42 32 2626
Fragments arranged by size:
2626 1549 472 179 147 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2188 2718 2964 669
Size: 988 281 250 530 246 3079
Fragments arranged by size:
3079 988 530 281 250 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5374
HpaI GTT'AAC
Cuts at: 2964 2964
Size: 5374
KasI G'GCGC_C
Cuts at: 2304 2304
Size: 5374
KpnI G_GTAC'C
Cuts at: 2699 2699
Size: 5374
MfeI C'AATT_G
Cuts at: 2973 2973
Size: 5374
MluI A'CGCG_T
Cuts at: 5250 5250
Size: 5374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2603 4488 4672 1305
Size: 1298 1885 184 2007
Fragments arranged by size:
2007 1885 1298 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3552
Fragments arranged by size:
3552 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2350 3332 3691 3850 518
Size: 648 1184 982 359 159 2042
Fragments arranged by size:
2042 1184 982 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2494 2790 3467 4615 4860 1606
Size: 888 296 677 1148 245 2120
Fragments arranged by size:
2120 1148 888 677 296 245
NarI GG'CG_CC
Cuts at: 2305 2305
Size: 5374
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4822
NdeI CA'TA_TG
Cuts at: 387 2379 387
Size: 1992 3382
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5374
NotI GC'GGCC_GC
Cuts at: 2821 2821
Size: 5374
NspI r_CATG'y
Cuts at: 1132 1229 2245 2732 4277 1132
Size: 97 1016 487 1545 2229
Fragments arranged by size:
2229 1545 1016 487 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5374
PciI A'CATG_T
Cuts at: 4273 4273
Size: 5374
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5374
PmeI GTTT'AAAC
Cuts at: 2678 2678
Size: 5374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 4956 4988 3443
Size: 32 1481 32 3829
Fragments arranged by size:
3829 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5374
PsiI TTA'TAA
Cuts at: 2944 2944
Size: 5374
PspOMI G'GGCC_C
Cuts at: 2328 2328
Size: 5374
PstI C_TGCA'G
Cuts at: 830 2295 2726 830
Size: 1465 431 3478
Fragments arranged by size:
3478 1465 431
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5374
PvuII CAG'CTG
Cuts at: 2494 2494
Size: 5374
SalI G'TCGA_C
Cuts at: 1936 2186 2716 1936
Size: 250 530 4594
Fragments arranged by size:
4594 530 250
SapI GCTCTTCn'nnn_
Cuts at: 2508 2508
Size: 5374
SbfI CC_TGCA'GG
Cuts at: 2295 2726 2295
Size: 431 4943
ScaI AGT'ACT
Cuts at: 3622 5334 3622
Size: 1712 3662
SfcI C'TryA_G
Cuts at: 826 1045 2291 2722 3857 4538 4729 826
Size: 219 1246 431 1135 681 191 1471
Fragments arranged by size:
1471 1246 1135 681 431 219 191
SfoI GGC'GCC
Cuts at: 2306 2306
Size: 5374
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5374
SmaI CCC'GGG
Cuts at: 1872 2701 1872
Size: 829 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2402 3478 4379 4641 820
Size: 197 926 459 1076 901 262 1553
Fragments arranged by size:
1553 1076 926 901 459 262 197
SnaBI TAC'GTA
Cuts at: 493 3165 493
Size: 2672 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5374
SphI G_CATG'C
Cuts at: 1132 2732 1132
Size: 1600 3774
SspI AAT'ATT
Cuts at: 5 52 3298 5
Size: 47 3246 2081
Fragments arranged by size:
3246 2081 47
StuI AGG'CCT
Cuts at: 1517 2609 1517
Size: 1092 4282
StyI C'CwwG_G
Cuts at: 513 1065 2460 2813 513
Size: 552 1395 353 3074
Fragments arranged by size:
3074 1395 552 353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3401 3418 3571 3756 4201 325
Size: 1255 1821 17 153 185 445 1498
Fragments arranged by size:
1821 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2223 3620 5332
Size: 274 80 33 51 1443 246 1397 1712
Cuts at: 5332 96
Size: 138
Fragments arranged by size:
1712 1443 1397 274 246 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2125 2157 5334 5366 2125
Size: 32 3177 32 2133
Fragments arranged by size:
3177 2133 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2038 1316
Size: 722 4652
XbaI T'CTAG_A
Cuts at: 2710 2710
Size: 5374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2111 2111
Size: 5374
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5374
XmaI C'CCGG_G
Cuts at: 1870 2699 1870
Size: 829 4545
XmnI GAAnn'nnTTC
Cuts at: 2630 3503 2630
Size: 873 4501
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3182 276
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SrfI SwaI