Restriction map for
pFN21AB8763
(Circular) (Six-base) MAPSORT of: pFN21AB8763.seq Check: 5094 from: 1 to: 5338
pFN21AB8763 5338 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3148 278
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2681 1937
Size: 744 4594
Acc65I G'GTAC_C
Cuts at: 2659 2659
Size: 5338
AclI AA'CG_TT
Cuts at: 1563 3465 3838 1563
Size: 1902 373 3063
Fragments arranged by size:
3063 1902 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3401 4785 5053 1632
Size: 1769 1384 268 1917
Fragments arranged by size:
1917 1769 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5141
AflIII A'CryG_T
Cuts at: 1203 4237 5214 1203
Size: 3034 977 1327
Fragments arranged by size:
3034 1327 977
AgeI A'CCGG_T
Cuts at: 4169 4169
Size: 5338
AhdI GACnn_n'nnGTC
Cuts at: 4067 4067
Size: 5338
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2402 1184
Size: 1218 4120
AlwNI CAG_nnn'CTG
Cuts at: 2533 3081 4653 2533
Size: 548 1572 3218
Fragments arranged by size:
3218 1572 548
ApaI G_GGCC'C
Cuts at: 2253 2497 2253
Size: 244 5094
ApaLI G'TGCA_C
Cuts at: 2261 2387 3395 4551 2261
Size: 126 1008 1156 3048
Fragments arranged by size:
3048 1156 1008 126
ApoI r'AATT_y
Cuts at: 1493 2647 2873 3025 5134 1493
Size: 1154 226 152 2109 1697
Fragments arranged by size:
2109 1697 1154 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3892 160
Size: 872 2860 1606
Fragments arranged by size:
2860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
AvaI C'yCGr_G
Cuts at: 1870 1943 2365 2663 1870
Size: 73 422 298 4545
Fragments arranged by size:
4545 422 298 73
BamHI G'GATC_C
Cuts at: 2668 3035 2668
Size: 367 4971
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2300 2473 2659 4114 618
Size: 325 217 627 513 173 186 1455 1842
Fragments arranged by size:
1842 1455 627 513 325 217 186 173
BanII G_rGCy'C
Cuts at: 1391 2061 2253 2295 2497 2621 2657 1391
Size: 670 192 42 202 124 36 4072
Fragments arranged by size:
4072 670 202 192 124 42 36
BbeI G_GCGC'C
Cuts at: 2477 2477
Size: 5338
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3529 3563 1903
Size: 34 1592 34 3678
Fragments arranged by size:
3678 1592 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3230 4440 3230
Size: 1210 4128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2065 3949 136
Size: 107 122 71 631 605 393 1884 1525
Fragments arranged by size:
1884 1525 631 605 393 122 107 71
BglII A'GATC_T
Cuts at: 5333 5333
Size: 5338
BlpI GC'TnA_GC
Cuts at: 2755 2755
Size: 5338
Bme1580I G_kGCm'C
Cuts at: 1790 2132 2253 2265 2303 2391 2497 3399 4555
Size: 342 121 12 38 88 106 902 1156
Cuts at: 4555 1790
Size: 2573
Fragments arranged by size:
2573 1156 902 342 121 106 88 38
12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2254 3069 4027
Size: 416 53 83 399 24 837 815 958
Cuts at: 4027 442
Size: 1753
Fragments arranged by size:
1753 958 837 815 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2477 3998 1239
Size: 1238 1521 2579
Fragments arranged by size:
2579 1521 1238
Bpu10I CC'TnA_GC
Cuts at: 2255 2348 2615 2255
Size: 93 267 4978
Fragments arranged by size:
4978 267 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3463 4328 4626 3463
Size: 865 298 4175
Fragments arranged by size:
4175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4001 882
Size: 617 2502 2219
Fragments arranged by size:
2502 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2467 3129 493
Size: 711 1263 662 2702
Fragments arranged by size:
2702 1263 711 662
BsaBI GATnn'nnATC
Cuts at: 5332 5332
Size: 5338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2229 2474 3145 3527
Size: 53 83 186 936 696 245 671 382
Cuts at: 3527 4162 275
Size: 635 1451
Fragments arranged by size:
1451 936 696 671 635 382 245 186
83 53
BsaWI w'CCGG_w
Cuts at: 3771 4169 4443 4590 5001 3771
Size: 398 274 147 411 4108
Fragments arranged by size:
4108 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2603 2633 1178
Size: 30 165 30 1200 30 3883
Fragments arranged by size:
3883 1200 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2074 2083 2086 1186
Size: 888 9 3 4438
Fragments arranged by size:
4438 888 9 3
BseYI C'CCAG_C
Cuts at: 2488 4541 5037 2488
Size: 2053 496 2789
Fragments arranged by size:
2789 2053 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4765
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2126 2404 2522 2788 3549 4577
Size: 275 186 125 278 118 266 761 1028
Cuts at: 4577 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 278 275 266 186 125
118
BsiHKAI G_wGCw'C
Cuts at: 2265 2391 2463 2621 3399 3484 4555 2265
Size: 126 72 158 778 85 1071 3048
Fragments arranged by size:
3048 1071 778 158 126 85 72
BsmI GAATG_Cn'
Cuts at: 2849 2942 2849
Size: 93 5245
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2132 2253 2265 2295 2303 2391
Size: 399 271 71 121 12 30 8 88
Cuts at: 2391 2463 2497 2621 2657 3399 3484 4555 1391
Size: 72 34 124 36 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 271 124 121 88
85 72 71 36 34 30 12 8
BspEI T'CCGG_A
Cuts at: 5001 5001
Size: 5338
BspHI T'CATG_A
Cuts at: 3227 4957 3227
Size: 1730 3608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2693 844
Size: 1849 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2278 3225 1394
Size: 429 455 947 3507
Fragments arranged by size:
3507 947 455 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3833 4007 65
Size: 1183 2585 174 1396
Fragments arranged by size:
2585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2196 2399 3982 4169 1700
Size: 110 386 203 1583 187 2869
Fragments arranged by size:
2869 1583 386 203 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5338
BssHII G'CGCG_C
Cuts at: 1925 2114 2209 2217 2284 1925
Size: 189 95 8 67 4979
Fragments arranged by size:
4979 189 95 67 8
BssSI C'ACGA_G
Cuts at: 2273 2374 3398 4410 2273
Size: 101 1024 1012 3201
Fragments arranged by size:
3201 1024 1012 101
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5338
BstBI TT'CG_AA
Cuts at: 4142 4142
Size: 5338
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5338
BstYI r'GATC_y
Cuts at: 1972 2668 3035 3420 3437 4889 4975 4987 5333
Size: 696 367 385 17 1452 86 12 346
Cuts at: 5333 1972
Size: 1977
Fragments arranged by size:
1977 1452 696 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2103 2120 2133 2295 2559 513
Size: 552 939 99 17 13 162 264 3292
Fragments arranged by size:
3292 939 552 264 162 99 17 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4599
Fragments arranged by size:
4599 683 56
BtsI GCAGTG_nn'
Cuts at: 2854 3648 3668 2854
Size: 794 20 4524
Fragments arranged by size:
4524 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5303
DraI TTT'AAA
Cuts at: 2642 2642
Size: 5338
DraIII CAC_nnn'GTG
Cuts at: 2467 2467
Size: 5338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2039 4345 809
Size: 507 723 2306 1802
Fragments arranged by size:
2306 1802 723 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2069 2123 2401
Size: 54 1425 50 275 18 239 54 278
Cuts at: 2401 2519 2553 2785 3674 5303 8
Size: 118 34 232 889 1629 43
Fragments arranged by size:
1629 1425 889 278 275 239 232 118
54 54 50 43 34 18
EagI C'GGCC_G
Cuts at: 1537 1812 2123 2401 2519 2785 1537
Size: 275 311 278 118 266 4090
Fragments arranged by size:
4090 311 278 275 266 118
EarI CTCTTCn'nnn_
Cuts at: 1353 2092 2450 3268 1353
Size: 739 358 818 3423
Fragments arranged by size:
3423 818 739 358
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3925 4299 4445 387
Size: 1064 138 2336 374 146 1280
Fragments arranged by size:
2336 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2619 2619
Size: 5338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2477 3401 3998 4785 5053 1239
Size: 393 845 924 597 787 268 1524
Fragments arranged by size:
1524 924 845 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2249 2250 2369 2493 2494 2249
Size: 1 119 124 1 5093
Fragments arranged by size:
5093 124 119 1 1
EcoRI G'AATT_C
Cuts at: 2647 3025 2647
Size: 378 4960
FseI GG_CCGG'CC
Cuts at: 2403 2403
Size: 5338
FspI TGC'GCA
Cuts at: 3844 3844
Size: 5338
HaeII r_GCGC'y
Cuts at: 1678 2477 2512 4485 1678
Size: 799 35 1973 2531
Fragments arranged by size:
2531 1973 799 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3979 4011 4053 4085 1373
Size: 32 135 32 2407 32 42 32 2626
Fragments arranged by size:
2626 2407 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2682 2928 669
Size: 988 281 744 246 3079
Fragments arranged by size:
3079 988 744 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5338
HpaI GTT'AAC
Cuts at: 2928 2928
Size: 5338
KasI G'GCGC_C
Cuts at: 2473 2473
Size: 5338
KpnI G_GTAC'C
Cuts at: 2663 2663
Size: 5338
MfeI C'AATT_G
Cuts at: 2937 2937
Size: 5338
MluI A'CGCG_T
Cuts at: 5214 5214
Size: 5338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4452 4636 1305
Size: 3147 184 2007
Fragments arranged by size:
3147 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2555 10
Size: 54 1425 343 723 2793
Fragments arranged by size:
2793 1425 723 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 2415 3296 3655 3814 518
Size: 648 1218 31 881 359 159 2042
Fragments arranged by size:
2042 1218 881 648 359 159 31
MspA1I CmG'CkG
Cuts at: 1606 2105 2122 2135 2329 2500 2524 2754 3431
Size: 499 17 13 194 171 24 230 677
Cuts at: 3431 4579 4824 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 499 245 230 194 171
24 17 13
NaeI GCC'GGC
Cuts at: 2401 2401
Size: 5338
NarI GG'CG_CC
Cuts at: 2474 2474
Size: 5338
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3847
Fragments arranged by size:
3847 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5338
NgoMIV G'CCGG_C
Cuts at: 2399 2399
Size: 5338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5338
NotI GC'GGCC_GC
Cuts at: 2519 2785 2519
Size: 266 5072
NspI r_CATG'y
Cuts at: 1132 1229 2488 2696 4241 1132
Size: 97 1259 208 1545 2229
Fragments arranged by size:
2229 1545 1259 208 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5338
PciI A'CATG_T
Cuts at: 4237 4237
Size: 5338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5338
PfoI T'CCnGG_A
Cuts at: 2223 2223
Size: 5338
PmeI GTTT'AAAC
Cuts at: 2642 2642
Size: 5338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 4920 4952 3407
Size: 32 1481 32 3793
Fragments arranged by size:
3793 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2369 2369
Size: 5338
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5338
PsiI TTA'TAA
Cuts at: 2908 2908
Size: 5338
PspOMI G'GGCC_C
Cuts at: 2249 2493 2249
Size: 244 5094
PstI C_TGCA'G
Cuts at: 830 2529 2690 830
Size: 1699 161 3478
Fragments arranged by size:
3478 1699 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5338
SacI G_AGCT'C
Cuts at: 2621 2621
Size: 5338
SacII CC_GC'GG
Cuts at: 2106 2123 2136 2106
Size: 17 13 5308
Fragments arranged by size:
5308 17 13
SalI G'TCGA_C
Cuts at: 1936 2680 1936
Size: 744 4594
SanDI GG'GwC_CC
Cuts at: 2369 2369
Size: 5338
SbfI CC_TGCA'GG
Cuts at: 2690 2690
Size: 5338
ScaI AGT'ACT
Cuts at: 3586 5298 3586
Size: 1712 3626
SexAI A'CCwGG_T
Cuts at: 2019 2019
Size: 5338
SfcI C'TryA_G
Cuts at: 826 1045 2525 2686 3821 4502 4693 826
Size: 219 1480 161 1135 681 191 1471
Fragments arranged by size:
1480 1471 1135 681 219 191 161
SfoI GGC'GCC
Cuts at: 2475 2475
Size: 5338
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5338
SmaI CCC'GGG
Cuts at: 1872 2665 1872
Size: 793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3442 4343 4605 820
Size: 197 926 1499 901 262 1553
Fragments arranged by size:
1553 1499 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3129 493
Size: 2636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5338
SphI G_CATG'C
Cuts at: 1132 2488 2696 1132
Size: 1356 208 3774
Fragments arranged by size:
3774 1356 208
SspI AAT'ATT
Cuts at: 5 52 3262 5
Size: 47 3210 2081
Fragments arranged by size:
3210 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5338
StyI C'CwwG_G
Cuts at: 513 1065 2004 2777 513
Size: 552 939 773 3074
Fragments arranged by size:
3074 939 773 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3365 3382 3535 3720 4165 325
Size: 1255 1785 17 153 185 445 1498
Fragments arranged by size:
1785 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3584 5296 96
Size: 274 80 33 51 1443 1607 1712 138
Fragments arranged by size:
1712 1607 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5298 5330 5298
Size: 32 5306
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5338
XbaI T'CTAG_A
Cuts at: 2674 2674
Size: 5338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2221 2221
Size: 5338
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5338
XmaI C'CCGG_G
Cuts at: 1870 2663 1870
Size: 793 4545
XmnI GAAnn'nnTTC
Cuts at: 3467 3467
Size: 5338
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3146 276
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV
FalI FspAI NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SapI SfiI SrfI SwaI