Restriction map for
pFN21AB8762
(Circular) (Six-base) MAPSORT of: pFN21AB8762.seq Check: 8326 from: 1 to: 5335
pFN21AB8762 5335 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3145 278
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2678 1937
Size: 741 4594
Acc65I G'GTAC_C
Cuts at: 2656 2656
Size: 5335
AclI AA'CG_TT
Cuts at: 1563 3462 3835 1563
Size: 1899 373 3063
Fragments arranged by size:
3063 1899 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2051 2244 3398 4782 5050 1632
Size: 419 193 1154 1384 268 1917
Fragments arranged by size:
1917 1384 1154 419 268 193
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5138
AflIII A'CryG_T
Cuts at: 1203 2154 4234 5211 1203
Size: 951 2080 977 1327
Fragments arranged by size:
2080 1327 977 951
AgeI A'CCGG_T
Cuts at: 4166 4166
Size: 5335
AhdI GACnn_n'nnGTC
Cuts at: 2077 4064 2077
Size: 1987 3348
AleI CACnn'nnGTG
Cuts at: 1166 2015 1166
Size: 849 4486
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5335
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2312 2344 2312
Size: 32 5303
AlwNI CAG_nnn'CTG
Cuts at: 2471 2611 3078 4650 2471
Size: 140 467 1572 3156
Fragments arranged by size:
3156 1572 467 140
ApaLI G'TGCA_C
Cuts at: 3392 4548 3392
Size: 1156 4179
ApoI r'AATT_y
Cuts at: 1493 2644 2870 3022 5131 1493
Size: 1151 226 152 2109 1697
Fragments arranged by size:
2109 1697 1151 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3889 160
Size: 872 2857 1606
Fragments arranged by size:
2857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
AvaI C'yCGr_G
Cuts at: 1870 1943 2660 1870
Size: 73 717 4545
Fragments arranged by size:
4545 717 73
BamHI G'GATC_C
Cuts at: 2665 3032 2665
Size: 367 4968
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2234 2656 4111 618
Size: 325 217 627 230 217 422 1455 1842
Fragments arranged by size:
1842 1455 627 422 325 230 217 217
BanII G_rGCy'C
Cuts at: 1391 2292 2520 2654 1391
Size: 901 228 134 4072
Fragments arranged by size:
4072 901 228 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4364
BbvCI CC'TCA_GC
Cuts at: 2418 2418
Size: 5335
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3526 3560 1903
Size: 34 1589 34 3678
Fragments arranged by size:
3678 1589 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3227 4437 3227
Size: 1210 4125
BclI T'GATC_A
Cuts at: 1548 2091 1548
Size: 543 4792
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3946 136
Size: 107 122 71 631 605 2274 1525
Fragments arranged by size:
2274 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5330 5330
Size: 5335
BlpI GC'TnA_GC
Cuts at: 2752 2752
Size: 5335
Bme1580I G_kGCm'C
Cuts at: 1790 2022 3396 4552 1790
Size: 232 1374 1156 2573
Fragments arranged by size:
2573 1374 1156 232
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3066 4024 442
Size: 416 53 83 399 24 1649 958 1753
Fragments arranged by size:
1753 1649 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5335
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 5335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2429 2476 3995 1239
Size: 1190 47 1519 2579
Fragments arranged by size:
2579 1519 1190 47
Bpu10I CC'TnA_GC
Cuts at: 2418 2418
Size: 5335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2300 3460 4325 4623 2300
Size: 1160 865 298 3012
Fragments arranged by size:
3012 1160 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2405 3998 882
Size: 617 906 1593 2219
Fragments arranged by size:
2219 1593 906 617
BsaAI yAC'GTr
Cuts at: 493 1204 2557 3126 493
Size: 711 1353 569 2702
Fragments arranged by size:
2702 1353 711 569
BsaBI GATnn'nnATC
Cuts at: 5329 5329
Size: 5335
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3142 3524 4159 275
Size: 53 83 186 936 1609 382 635 1451
Fragments arranged by size:
1609 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3768 4166 4440 4587 4998 3768
Size: 398 274 147 411 4105
Fragments arranged by size:
4105 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2312 2342 2430 2460 1178
Size: 30 165 30 909 30 88 30 4053
Fragments arranged by size:
4053 909 165 88 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2528 1186
Size: 1342 3993
BseYI C'CCAG_C
Cuts at: 2104 4538 5034 2104
Size: 2434 496 2405
Fragments arranged by size:
2434 2405 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2387 2537 1163
Size: 573 651 150 3961
Fragments arranged by size:
3961 651 573 150
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2785 3546 4574 1540
Size: 275 186 784 761 1028 2301
Fragments arranged by size:
2301 1028 784 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2292 3396 3481 4552 2292
Size: 1104 85 1071 3075
Fragments arranged by size:
3075 1104 1071 85
BsmI GAATG_Cn'
Cuts at: 2846 2939 2846
Size: 93 5242
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2292 2520 2654 3396 3481 4552
Size: 399 232 270 228 134 742 85 1071
Cuts at: 4552 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 270 232 228 134
85
BspEI T'CCGG_A
Cuts at: 4998 4998
Size: 5335
BspHI T'CATG_A
Cuts at: 3224 4954 3224
Size: 1730 3605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2082 2690 844
Size: 1238 608 3489
Fragments arranged by size:
3489 1238 608
BsrBI CCG'CTC
Cuts at: 1394 1823 3222 1394
Size: 429 1399 3507
Fragments arranged by size:
3507 1399 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3830 4004 65
Size: 1183 2582 174 1396
Fragments arranged by size:
2582 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3979 4166 1700
Size: 110 2169 187 2869
Fragments arranged by size:
2869 2169 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5335
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5335
BssSI C'ACGA_G
Cuts at: 3395 4407 3395
Size: 1012 4323
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5335
BstBI TT'CG_AA
Cuts at: 4139 4139
Size: 5335
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5335
BstYI r'GATC_y
Cuts at: 1972 2625 2665 3032 3417 3434 4886 4972 4984
Size: 653 40 367 385 17 1452 86 12
Cuts at: 4984 5330 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 653 385 367 346 86 40
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2208 513
Size: 552 1143 3640
Fragments arranged by size:
3640 1143 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4596
Fragments arranged by size:
4596 683 56
BtsI GCAGTG_nn'
Cuts at: 2143 2155 2851 3645 3665 2143
Size: 12 696 794 20 3813
Fragments arranged by size:
3813 794 696 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5300
DraI TTT'AAA
Cuts at: 2639 2639
Size: 5335
DraIII CAC_nnn'GTG
Cuts at: 2277 2277
Size: 5335
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2500 4342 809
Size: 507 1184 1842 1802
Fragments arranged by size:
1842 1802 1184 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2782 3671 5300
Size: 54 1425 50 275 18 952 889 1629
Cuts at: 5300 8
Size: 43
Fragments arranged by size:
1629 1425 952 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2782 1537
Size: 275 970 4090
Fragments arranged by size:
4090 970 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3265 1353
Size: 1912 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2105 3922 4296 4442 387
Size: 1064 138 516 1817 374 146 1280
Fragments arranged by size:
1817 1280 1064 516 374 146 138
EcoICRI GAG'CTC
Cuts at: 2290 2290
Size: 5335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2244 2429 2476 3398 3995 4782
Size: 393 419 193 185 47 922 597 787
Cuts at: 4782 5050 1239
Size: 268 1524
Fragments arranged by size:
1524 922 787 597 419 393 268 193
185 47
EcoO109I rG'GnC_Cy
Cuts at: 2071 2219 2567 2608 2071
Size: 148 348 41 4798
Fragments arranged by size:
4798 348 148 41
EcoRI G'AATT_C
Cuts at: 2644 3022 2644
Size: 378 4957
FspI TGC'GCA
Cuts at: 3841 3841
Size: 5335
HaeII r_GCGC'y
Cuts at: 1678 4482 1678
Size: 2804 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2063 2095 2288 2320 2587
Size: 32 135 32 491 32 193 32 267
Cuts at: 2587 2619 3976 4008 4050 4082 1373
Size: 32 1357 32 42 32 2626
Fragments arranged by size:
2626 1357 491 267 193 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2679 2925 669
Size: 988 281 741 246 3079
Fragments arranged by size:
3079 988 741 281 246
HindIII A'AGCT_T
Cuts at: 748 2063 748
Size: 1315 4020
HpaI GTT'AAC
Cuts at: 2925 2925
Size: 5335
KpnI G_GTAC'C
Cuts at: 2660 2660
Size: 5335
MfeI C'AATT_G
Cuts at: 2934 2934
Size: 5335
MluI A'CGCG_T
Cuts at: 5211 5211
Size: 5335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4449 4633 1305
Size: 3144 184 2007
Fragments arranged by size:
3144 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3513
Fragments arranged by size:
3513 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2015 3293 3652 3811 518
Size: 648 849 1278 359 159 2042
Fragments arranged by size:
2042 1278 849 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2104 2751 3428 4576 4821 1606
Size: 498 647 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 647 498 245
NcoI C'CATG_G
Cuts at: 513 1065 2208 513
Size: 552 1143 3640
Fragments arranged by size:
3640 1143 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5335
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5335
NotI GC'GGCC_GC
Cuts at: 2782 2782
Size: 5335
NspI r_CATG'y
Cuts at: 1132 1229 2158 2623 2693 4238 1132
Size: 97 929 465 70 1545 2229
Fragments arranged by size:
2229 1545 929 465 97 70
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5335
PciI A'CATG_T
Cuts at: 2154 4234 2154
Size: 2080 3255
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2471 1108
Size: 1363 3972
PfoI T'CCnGG_A
Cuts at: 2292 2577 2292
Size: 285 5050
PmeI GTTT'AAAC
Cuts at: 2639 2639
Size: 5335
PmlI CAC'GTG
Cuts at: 2557 2557
Size: 5335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2312 2344 3404 3436 4917 4949 2312
Size: 32 1060 32 1481 32 2698
Fragments arranged by size:
2698 1481 1060 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2071 2608 2071
Size: 537 4798
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5335
PsiI TTA'TAA
Cuts at: 2905 2905
Size: 5335
PstI C_TGCA'G
Cuts at: 830 2181 2595 2687 830
Size: 1351 414 92 3478
Fragments arranged by size:
3478 1351 414 92
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5335
SacI G_AGCT'C
Cuts at: 2292 2292
Size: 5335
SalI G'TCGA_C
Cuts at: 1936 2677 1936
Size: 741 4594
SbfI CC_TGCA'GG
Cuts at: 2181 2687 2181
Size: 506 4829
ScaI AGT'ACT
Cuts at: 3583 5295 3583
Size: 1712 3623
SfcI C'TryA_G
Cuts at: 826 1045 2177 2591 2683 3818 4499 4690 826
Size: 219 1132 414 92 1135 681 191 1471
Fragments arranged by size:
1471 1135 1132 681 414 219 191 92
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5335
SmaI CCC'GGG
Cuts at: 1872 2662 1872
Size: 790 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2315 3439 4340 4602 820
Size: 197 926 372 1124 901 262 1553
Fragments arranged by size:
1553 1124 926 901 372 262 197
SnaBI TAC'GTA
Cuts at: 493 3126 493
Size: 2633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5335
SphI G_CATG'C
Cuts at: 1132 2623 2693 1132
Size: 1491 70 3774
Fragments arranged by size:
3774 1491 70
SspI AAT'ATT
Cuts at: 5 52 3259 5
Size: 47 3207 2081
Fragments arranged by size:
3207 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5335
StyI C'CwwG_G
Cuts at: 513 1065 2013 2208 2774 513
Size: 552 948 195 566 3074
Fragments arranged by size:
3074 948 566 552 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2401 3362 3379 3532 3717 4162 325
Size: 1255 821 961 17 153 185 445 1498
Fragments arranged by size:
1498 1255 961 821 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3581 5293 96
Size: 274 80 33 51 1443 1604 1712 138
Fragments arranged by size:
1712 1604 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 5295 5327 2430
Size: 32 2833 32 2438
Fragments arranged by size:
2833 2438 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5335
XbaI T'CTAG_A
Cuts at: 2671 2671
Size: 5335
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5335
XmaI C'CCGG_G
Cuts at: 1870 2660 1870
Size: 790 4545
XmnI GAAnn'nnTTC
Cuts at: 3464 3464
Size: 5335
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3143 276
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI