Restriction map for
pFN21AB8749
(Circular) (Six-base) MAPSORT of: pFN21AB8749.seq Check: 7763 from: 1 to: 5959
pFN21AB8749 5959 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2139 3302 1937
Size: 202 1163 4594
Fragments arranged by size:
4594 1163 202
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2445 4022 5406 5674 1632
Size: 813 1577 1384 268 1917
Fragments arranged by size:
1917 1577 1384 813 268
AflII C'TTAA_G
Cuts at: 820 1017 2470 820
Size: 197 1453 4309
Fragments arranged by size:
4309 1453 197
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 2198 1166
Size: 1032 4927
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AlwNI CAG_nnn'CTG
Cuts at: 3150 3702 5274 3150
Size: 552 1572 3835
Fragments arranged by size:
3835 1572 552
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 2913 3268 3494 3646 5755 1493
Size: 1420 355 226 152 2109 1697
Fragments arranged by size:
2109 1697 1420 355 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2907 3284 1870
Size: 73 964 377 4545
Fragments arranged by size:
4545 964 377 73
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2214 3280 4735 618
Size: 325 217 627 427 1066 1455 1842
Fragments arranged by size:
1842 1455 1066 627 427 325 217
BanII G_rGCy'C
Cuts at: 1391 2542 2659 2789 3278 1391
Size: 1151 117 130 489 4072
Fragments arranged by size:
4072 1151 489 130 117
BbeI G_GCGC'C
Cuts at: 2218 2218
Size: 5959
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2110 2685 928
Size: 971 211 575 4202
Fragments arranged by size:
4202 971 575 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3072 3106 4150 4184 1903
Size: 34 1135 34 1044 34 3678
Fragments arranged by size:
3678 1135 1044 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2519 4570 136
Size: 107 122 71 631 605 847 2051 1525
Fragments arranged by size:
2051 1525 847 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 4020 5176 1790
Size: 2230 1156 2573
Fragments arranged by size:
2573 2230 1156
BmgBI CAC'GTC
Cuts at: 1318 3096 1318
Size: 1778 4181
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3690 4648 442
Size: 416 53 83 399 24 2273 958 1753
Fragments arranged by size:
2273 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2933 2933
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2444 4619 1239
Size: 1205 2175 2579
Fragments arranged by size:
2579 2175 1205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4084 4949 5247 4084
Size: 865 298 4796
Fragments arranged by size:
4796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2378 3161 4622 882
Size: 617 879 783 1461 2219
Fragments arranged by size:
2219 1461 879 783 617
BsaAI yAC'GTr
Cuts at: 493 1204 2635 3750 493
Size: 711 1431 1115 2702
Fragments arranged by size:
2702 1431 1115 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2215 3766 4148 4783
Size: 53 83 186 936 682 1551 382 635
Cuts at: 4783 275
Size: 1451
Fragments arranged by size:
1551 1451 936 682 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5734
Fragments arranged by size:
5734 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3179 1186
Size: 1993 3966
BseYI C'CCAG_C
Cuts at: 5162 5658 5162
Size: 496 5463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3076 3150 1163
Size: 573 1340 74 3972
Fragments arranged by size:
3972 1340 573 74
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 2448 4020 4105 5176 2076
Size: 372 1572 85 1071 2859
Fragments arranged by size:
2859 1572 1071 372 85
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
BsmBI CGTCTCn'nnnn_
Cuts at: 3106 3106
Size: 5959
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2448 2542 2659 2789 3278 4020
Size: 399 286 372 94 117 130 489 742
Cuts at: 4020 4105 5176 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 489 399 372 286 130
117 94 85
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3314 844
Size: 2470 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4454 4628 65
Size: 1183 3206 174 1396
Fragments arranged by size:
3206 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4603 4790 1700
Size: 110 2793 187 2869
Fragments arranged by size:
2869 2793 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 2254 4019 5031 2254
Size: 1765 1012 3182
Fragments arranged by size:
3182 1765 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 2746 1184
Size: 1562 4397
BstXI CCAn_nnnn'nTGG
Cuts at: 2200 3034 2200
Size: 834 5125
BstYI r'GATC_y
Cuts at: 1972 3289 3656 4041 4058 5510 5596 5608 5954
Size: 1317 367 385 17 1452 86 12 346
Cuts at: 5954 1972
Size: 1977
Fragments arranged by size:
1977 1452 1317 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3162 3162
Size: 5959
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5407
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 3028 3475 4269 4289 3028
Size: 447 794 20 4698
Fragments arranged by size:
4698 794 447 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2616 2651 529
Size: 35 2052 35 3837
Fragments arranged by size:
3837 2052 35 35
DraI TTT'AAA
Cuts at: 2710 3231 3263 2710
Size: 521 32 5406
Fragments arranged by size:
5406 521 32
DraIII CAC_nnn'GTG
Cuts at: 2211 2336 2211
Size: 125 5834
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2439 2511 2624
Size: 54 1425 50 275 18 609 72 113
Cuts at: 2624 3406 4295 5924 8
Size: 782 889 1629 43
Fragments arranged by size:
1629 1425 889 782 609 275 113 72
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2089 3889 1353
Size: 731 5 1800 3423
Fragments arranged by size:
3423 1800 731 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3169 4546 4920 5066 387
Size: 1064 138 1580 1377 374 146 1280
Fragments arranged by size:
1580 1377 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2444 2445 4022 4619 5406 5674 1239
Size: 393 812 1 1577 597 787 268 1524
Fragments arranged by size:
1577 1524 812 787 597 393 268 1
EcoO109I rG'GnC_Cy
Cuts at: 2364 2364
Size: 5959
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 2218 5106 1678
Size: 540 2888 2531
Fragments arranged by size:
2888 2531 540
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4600 4632 4674 4706 1373
Size: 32 135 32 3028 32 42 32 2626
Fragments arranged by size:
3028 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2182 2341 2728 3303 3549 669
Size: 988 281 244 159 387 575 246 3079
Fragments arranged by size:
3079 988 575 387 281 246 244 159
HindIII A'AGCT_T
Cuts at: 748 2591 748
Size: 1843 4116
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KasI G'GCGC_C
Cuts at: 2214 2214
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3032 3558 3032
Size: 526 5433
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3078 5073 5257 1305
Size: 1773 1995 184 2007
Fragments arranged by size:
2007 1995 1773 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2513 10
Size: 54 1425 343 681 3456
Fragments arranged by size:
3456 1425 681 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2121 2198 3917 4276 4435 518
Size: 648 955 77 1719 359 159 2042
Fragments arranged by size:
2042 1719 955 648 359 159 77
MspA1I CmG'CkG
Cuts at: 1606 2130 3150 3375 4052 5200 5445 1606
Size: 524 1020 225 677 1148 245 2120
Fragments arranged by size:
2120 1148 1020 677 524 245 225
NarI GG'CG_CC
Cuts at: 2215 2215
Size: 5959
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5407
NdeI CA'TA_TG
Cuts at: 387 2220 387
Size: 1833 4126
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3317 4862 1132
Size: 97 2088 1545 2229
Fragments arranged by size:
2229 2088 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PmlI CAC'GTG
Cuts at: 2635 2635
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3118 1613
Size: 1505 4454
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 3311 830
Size: 2481 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
PvuII CAG'CTG
Cuts at: 2130 3150 2130
Size: 1020 4939
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SapI GCTCTTCn'nnn_
Cuts at: 2089 2089
Size: 5959
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 3243 3307 4442 5123 5314 826
Size: 219 2198 64 1135 681 191 1471
Fragments arranged by size:
2198 1471 1135 681 219 191 64
SfoI GGC'GCC
Cuts at: 2216 2216
Size: 5959
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2470 4063 4964 5226 820
Size: 197 926 527 1593 901 262 1553
Fragments arranged by size:
1593 1553 926 901 527 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2508 3105 3398 513
Size: 552 1443 597 293 3074
Fragments arranged by size:
3074 1443 597 552 293
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3986 4003 4156 4341 4786 325
Size: 1255 2406 17 153 185 445 1498
Fragments arranged by size:
2406 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4205 5917 96
Size: 274 80 33 51 1443 2228 1712 138
Fragments arranged by size:
2228 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2160 2192 2195 2227 5919 5951 2160
Size: 32 3 32 3692 32 2168
Fragments arranged by size:
3692 2168 32 32 32 3
Tth111I GACn'n_nGTC
Cuts at: 1316 2345 1316
Size: 1029 4930
XbaI T'CTAG_A
Cuts at: 2888 3295 2888
Size: 407 5552
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SrfI SwaI XcmI