Restriction map for
pFN21AB8742
(Circular) (Six-base) MAPSORT of: pFN21AB8742.seq Check: 7651 from: 1 to: 5830
pFN21AB8742 5830 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3173 1937
Size: 1236 4594
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3893 5277 5545 1632
Size: 2261 1384 268 1917
Fragments arranged by size:
2261 1917 1384 268
AfeI AGC'GCT
Cuts at: 2878 2878
Size: 5830
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 4729 5706 1203
Size: 3526 977 1327
Fragments arranged by size:
3526 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 2087 4559 2087
Size: 2472 3358
AleI CACnn'nnGTG
Cuts at: 1166 2225 1166
Size: 1059 4771
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2669 1184
Size: 1485 4345
AlwNI CAG_nnn'CTG
Cuts at: 2550 3041 3573 5145 2550
Size: 491 532 1572 3235
Fragments arranged by size:
3235 1572 532 491
ApaLI G'TGCA_C
Cuts at: 3887 5043 3887
Size: 1156 4674
ApoI r'AATT_y
Cuts at: 1493 2711 3139 3365 3517 5626 1493
Size: 1218 428 226 152 2109 1697
Fragments arranged by size:
2109 1697 1218 428 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 3056 3155 1870
Size: 73 1113 99 4545
Fragments arranged by size:
4545 1113 99 73
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3151 4606 618
Size: 325 217 627 1364 1455 1842
Fragments arranged by size:
1842 1455 1364 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2070 2304 2741 2792 3149 1391
Size: 679 234 437 51 357 4072
Fragments arranged by size:
4072 679 437 357 234 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4859
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4021 4055 1903
Size: 34 2084 34 3678
Fragments arranged by size:
3678 2084 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 4932 3722
Size: 1210 4620
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4441 136
Size: 107 122 71 631 605 2769 1525
Fragments arranged by size:
2769 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2475 5825 2475
Size: 3350 2480
BlpI GC'TnA_GC
Cuts at: 3247 3247
Size: 5830
Bme1580I G_kGCm'C
Cuts at: 1790 2109 3891 5047 1790
Size: 319 1782 1156 2573
Fragments arranged by size:
2573 1782 1156 319
BmgBI CAC'GTC
Cuts at: 1318 2665 1318
Size: 1347 4483
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2358 2932 3561
Size: 416 53 83 399 24 941 574 629
Cuts at: 3561 4519 442
Size: 958 1753
Fragments arranged by size:
1753 958 941 629 574 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4490 1239
Size: 3251 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 3955 4820 5118 2054
Size: 1901 865 298 2766
Fragments arranged by size:
2766 1901 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2525 2793 4493 882
Size: 617 1026 268 1700 2219
Fragments arranged by size:
2219 1700 1026 617 268
BsaAI yAC'GTr
Cuts at: 493 1204 2719 3621 493
Size: 711 1515 902 2702
Fragments arranged by size:
2702 1515 902 711
BsaBI GATnn'nnATC
Cuts at: 2474 2480 5824 2474
Size: 6 3344 2480
Fragments arranged by size:
3344 2480 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3637 4019 4654 275
Size: 53 83 186 936 2104 382 635 1451
Fragments arranged by size:
2104 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2486 4263 4661 4935 5082 5493 2486
Size: 1777 398 274 147 411 2823
Fragments arranged by size:
2823 1777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2708 2738 2816 2846 1178
Size: 30 165 30 1305 30 78 30 4162
Fragments arranged by size:
4162 1305 165 78 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2681 2716 2719 2840 3074 1186
Size: 1495 35 3 121 234 3942
Fragments arranged by size:
3942 1495 234 121 35 3
BseYI C'CCAG_C
Cuts at: 2057 2063 5033 5529 2057
Size: 6 2970 496 2358
Fragments arranged by size:
2970 2358 496 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2574 3054 1163
Size: 573 838 480 3939
Fragments arranged by size:
3939 838 573 480
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3280 4041 5069 1540
Size: 275 186 1279 761 1028 2301
Fragments arranged by size:
2301 1279 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3891 3976 5047 3891
Size: 85 1071 4674
Fragments arranged by size:
4674 1071 85
BsmI GAATG_Cn'
Cuts at: 3341 3434 3341
Size: 93 5737
BsmBI CGTCTCn'nnnn_
Cuts at: 2777 2777
Size: 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2109 2304 2741 2792 3149 3891
Size: 399 280 39 195 437 51 357 742
Cuts at: 3891 3976 5047 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 437 399 357 280 195
85 51 39
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 2328 3719 5449 2328
Size: 1391 1730 2709
Fragments arranged by size:
2709 1730 1391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2163 3185 844
Size: 1319 1022 3489
Fragments arranged by size:
3489 1319 1022
BsrBI CCG'CTC
Cuts at: 1394 1823 2683 3717 1394
Size: 429 860 1034 3507
Fragments arranged by size:
3507 1034 860 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3130 4325 4499 65
Size: 1183 1882 1195 174 1396
Fragments arranged by size:
1882 1396 1195 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4474 4661 1700
Size: 110 2664 187 2869
Fragments arranged by size:
2869 2664 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 3890 4902 3890
Size: 1012 4818
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5830
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5830
BstXI CCAn_nnnn'nTGG
Cuts at: 2642 2642
Size: 5830
BstYI r'GATC_y
Cuts at: 1972 2475 3160 3527 3912 3929 5381 5467 5479
Size: 503 685 367 385 17 1452 86 12
Cuts at: 5479 5825 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 685 503 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4339
Fragments arranged by size:
4339 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2251 487
Size: 683 56 1025 4066
Fragments arranged by size:
4066 1025 683 56
BtsI GCAGTG_nn'
Cuts at: 2052 3346 4140 4160 2052
Size: 1294 794 20 3722
Fragments arranged by size:
3722 1294 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2147 2182 529
Size: 35 1583 35 4177
Fragments arranged by size:
4177 1583 35 35
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4837 809
Size: 507 3521 1802
Fragments arranged by size:
3521 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2499 3277 4166
Size: 54 1425 50 275 18 669 778 889
Cuts at: 4166 5795 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 778 669 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3277 1537
Size: 275 1465 4090
Fragments arranged by size:
4090 1465 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2609 3760 1353
Size: 1256 1151 3423
Fragments arranged by size:
3423 1256 1151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4417 4791 4937 387
Size: 1064 138 2828 374 146 1280
Fragments arranged by size:
2828 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3893 4490 5277 5545 1239
Size: 393 2261 597 787 268 1524
Fragments arranged by size:
2261 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2602 2638 2602
Size: 36 5794
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 2880 4977 1678
Size: 1202 2097 2531
Fragments arranged by size:
2531 2097 1202
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2073 2105 2187 2219 2714
Size: 32 135 32 501 32 82 32 495
Cuts at: 2714 2746 4471 4503 4545 4577 1373
Size: 32 1725 32 42 32 2626
Fragments arranged by size:
2626 1725 501 495 135 82 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3174 3420 669
Size: 988 281 1236 246 3079
Fragments arranged by size:
3079 1236 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5830
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4944 5128 1305
Size: 3639 184 2007
Fragments arranged by size:
3639 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2501 10
Size: 54 1425 343 669 3339
Fragments arranged by size:
3339 1425 669 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 2225 2333 3788 4147 4306 518
Size: 648 858 201 108 1455 359 159 2042
Fragments arranged by size:
2042 1455 858 648 359 201 159 108
MspA1I CmG'CkG
Cuts at: 1606 3246 3923 5071 5316 1606
Size: 1640 677 1148 245 2120
Fragments arranged by size:
2120 1640 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4339
Fragments arranged by size:
4339 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 2029 3188 4733 1132
Size: 97 800 1159 1545 2229
Fragments arranged by size:
2229 1545 1159 800 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5830
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2776 1108
Size: 1668 4162
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PmlI CAC'GTG
Cuts at: 2719 2719
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 5412 5444 3899
Size: 32 1481 32 4285
Fragments arranged by size:
4285 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2663 1613
Size: 1050 4780
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PspXI vC'TCGA_Gb
Cuts at: 3056 3056
Size: 5830
PstI C_TGCA'G
Cuts at: 830 2658 3182 830
Size: 1828 524 3478
Fragments arranged by size:
3478 1828 524
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SapI GCTCTTCn'nnn_
Cuts at: 2609 2609
Size: 5830
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SexAI A'CCwGG_T
Cuts at: 2234 2234
Size: 5830
SfcI C'TryA_G
Cuts at: 826 1045 2654 3178 4313 4994 5185 826
Size: 219 1609 524 1135 681 191 1471
Fragments arranged by size:
1609 1471 1135 681 524 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 3157 1872
Size: 1285 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2069 3056 3934 4835 5097 820
Size: 197 926 126 987 878 901 262 1553
Fragments arranged by size:
1553 987 926 901 878 262 197 126
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5830
StyI C'CwwG_G
Cuts at: 513 1065 2004 2670 3269 513
Size: 552 939 666 599 3074
Fragments arranged by size:
3074 939 666 599 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3857 3874 4027 4212 4657 325
Size: 1255 2277 17 153 185 445 1498
Fragments arranged by size:
2277 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3066 4076 5788
Size: 274 80 33 51 1443 1089 1010 1712
Cuts at: 5788 96
Size: 138
Fragments arranged by size:
1712 1443 1089 1010 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2708 2740 2958 2990 5790 5822 2708
Size: 32 218 32 2800 32 2716
Fragments arranged by size:
2800 2716 218 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5830
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 2384 2437 2038
Size: 346 53 5431
Fragments arranged by size:
5431 346 53
XhoI C'TCGA_G
Cuts at: 1943 3056 1943
Size: 1113 4717
XmaI C'CCGG_G
Cuts at: 1870 3155 1870
Size: 1285 4545
XmnI GAAnn'nnTTC
Cuts at: 3000 3959 3000
Size: 959 4871
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PstI
PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PsrI
PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI