Restriction map for
pFN21AB8741
(Circular) (Six-base) MAPSORT of: pFN21AB8741.seq Check: 6811 from: 1 to: 5122
pFN21AB8741 5122 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2932 278
Size: 53 83 186 936 1396 2468
Fragments arranged by size:
2468 1396 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2465 1937
Size: 528 4594
Acc65I G'GTAC_C
Cuts at: 2443 2443
Size: 5122
AclI AA'CG_TT
Cuts at: 1563 3249 3622 1563
Size: 1686 373 3063
Fragments arranged by size:
3063 1686 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2352 3185 4569 4837 1632
Size: 720 833 1384 268 1917
Fragments arranged by size:
1917 1384 833 720 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4925
AflIII A'CryG_T
Cuts at: 1203 4021 4998 1203
Size: 2818 977 1327
Fragments arranged by size:
2818 1327 977
AgeI A'CCGG_T
Cuts at: 3953 3953
Size: 5122
AhdI GACnn_n'nnGTC
Cuts at: 3851 3851
Size: 5122
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5122
AlwNI CAG_nnn'CTG
Cuts at: 2165 2865 4437 2165
Size: 700 1572 2850
Fragments arranged by size:
2850 1572 700
ApaLI G'TGCA_C
Cuts at: 3179 4335 3179
Size: 1156 3966
ApoI r'AATT_y
Cuts at: 1493 2101 2203 2227 2294 2431 2657 2809 4918
Size: 608 102 24 67 137 226 152 2109
Cuts at: 4918 1493
Size: 1697
Fragments arranged by size:
2109 1697 608 226 152 137 102 67
24
AseI AT'TA_AT
Cuts at: 160 1032 3676 160
Size: 872 2644 1606
Fragments arranged by size:
2644 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5122
AvaI C'yCGr_G
Cuts at: 1870 1943 2447 1870
Size: 73 504 4545
Fragments arranged by size:
4545 504 73
BamHI G'GATC_C
Cuts at: 2452 2819 2452
Size: 367 4755
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2288 2443 3898 618
Size: 325 217 627 501 155 1455 1842
Fragments arranged by size:
1842 1455 627 501 325 217 155
BanII G_rGCy'C
Cuts at: 1391 2441 1391
Size: 1050 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2093 928
Size: 971 194 3957
Fragments arranged by size:
3957 971 194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3313 3347 1903
Size: 34 1376 34 3678
Fragments arranged by size:
3678 1376 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3014 4224 3014
Size: 1210 3912
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5122
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2174 3733 136
Size: 107 122 71 631 605 502 1559 1525
Fragments arranged by size:
1559 1525 631 605 502 122 107 71
BglII A'GATC_T
Cuts at: 5117 5117
Size: 5122
BlpI GC'TnA_GC
Cuts at: 2539 2539
Size: 5122
Bme1580I G_kGCm'C
Cuts at: 1790 3183 4339 1790
Size: 1393 1156 2573
Fragments arranged by size:
2573 1393 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5122
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2353 2853 3811
Size: 416 53 83 399 24 936 500 958
Cuts at: 3811 442
Size: 1753
Fragments arranged by size:
1753 958 936 500 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3782 1239
Size: 2543 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2250 3247 4112 4410 2250
Size: 997 865 298 2962
Fragments arranged by size:
2962 997 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3785 882
Size: 617 2286 2219
Fragments arranged by size:
2286 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2913 493
Size: 711 1709 2702
Fragments arranged by size:
2702 1709 711
BsaBI GATnn'nnATC
Cuts at: 5116 5116
Size: 5122
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2929 3311 3946 275
Size: 53 83 186 936 1396 382 635 1451
Fragments arranged by size:
1451 1396 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3555 3953 4227 4374 4785 3555
Size: 398 274 147 411 3892
Fragments arranged by size:
3892 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4897
Fragments arranged by size:
4897 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5122
BseYI C'CCAG_C
Cuts at: 4325 4821 4325
Size: 496 4626
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4549
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2572 3333 4361 1540
Size: 275 186 571 761 1028 2301
Fragments arranged by size:
2301 1028 761 571 275 186
BsiHKAI G_wGCw'C
Cuts at: 3183 3268 4339 3183
Size: 85 1071 3966
Fragments arranged by size:
3966 1071 85
BsmI GAATG_Cn'
Cuts at: 2633 2726 2633
Size: 93 5029
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2441 3183 3268 4339 1391
Size: 399 651 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 651 399 85
BspEI T'CCGG_A
Cuts at: 4785 4785
Size: 5122
BspHI T'CATG_A
Cuts at: 3011 4741 3011
Size: 1730 3392
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2477 844
Size: 1633 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3009 1394
Size: 429 1186 3507
Fragments arranged by size:
3507 1186 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3617 3791 65
Size: 1183 2369 174 1396
Fragments arranged by size:
2369 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3766 3953 1700
Size: 110 1956 187 2869
Fragments arranged by size:
2869 1956 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5122
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5122
BssSI C'ACGA_G
Cuts at: 3182 4194 3182
Size: 1012 4110
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2179 794
Size: 1385 3737
BstBI TT'CG_AA
Cuts at: 3926 3926
Size: 5122
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5122
BstXI CCAn_nnnn'nTGG
Cuts at: 2343 2343
Size: 5122
BstYI r'GATC_y
Cuts at: 1972 2452 2819 3204 3221 4673 4759 4771 5117
Size: 480 367 385 17 1452 86 12 346
Cuts at: 5117 1972
Size: 1977
Fragments arranged by size:
1977 1452 480 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3631
Fragments arranged by size:
3631 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4383
Fragments arranged by size:
4383 683 56
BtsI GCAGTG_nn'
Cuts at: 2638 3432 3452 2638
Size: 794 20 4308
Fragments arranged by size:
4308 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5122
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5087
DraI TTT'AAA
Cuts at: 2260 2426 2260
Size: 166 4956
DraIII CAC_nnn'GTG
Cuts at: 2318 2318
Size: 5122
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4129 809
Size: 507 2813 1802
Fragments arranged by size:
2813 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2569 3458 5087
Size: 54 1425 50 275 18 739 889 1629
Cuts at: 5087 8
Size: 43
Fragments arranged by size:
1629 1425 889 739 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2569 1537
Size: 275 757 4090
Fragments arranged by size:
4090 757 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3052 1353
Size: 1699 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3709 4083 4229 387
Size: 1064 138 2120 374 146 1280
Fragments arranged by size:
2120 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2352 3185 3782 4569 4837 1239
Size: 393 720 833 597 787 268 1524
Fragments arranged by size:
1524 833 787 720 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2243 2347 2243
Size: 104 5018
EcoRI G'AATT_C
Cuts at: 2101 2227 2431 2809 2101
Size: 126 204 378 4414
Fragments arranged by size:
4414 378 204 126
FspI TGC'GCA
Cuts at: 3628 3628
Size: 5122
HaeII r_GCGC'y
Cuts at: 1678 4269 1678
Size: 2591 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2294 2326 3763 3795 3837
Size: 32 135 32 722 32 1437 32 42
Cuts at: 3837 3869 1373
Size: 32 2626
Fragments arranged by size:
2626 1437 722 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2466 2712 669
Size: 988 281 528 246 3079
Fragments arranged by size:
3079 988 528 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5122
HpaI GTT'AAC
Cuts at: 2712 2712
Size: 5122
KpnI G_GTAC'C
Cuts at: 2447 2447
Size: 5122
MfeI C'AATT_G
Cuts at: 2721 2721
Size: 5122
MluI A'CGCG_T
Cuts at: 4998 4998
Size: 5122
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2204 4236 4420 1305
Size: 899 2032 184 2007
Fragments arranged by size:
2032 2007 899 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3300
Fragments arranged by size:
3300 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3080 3439 3598 518
Size: 648 1914 359 159 2042
Fragments arranged by size:
2042 1914 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2165 2538 3215 4363 4608 1606
Size: 559 373 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 559 373 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3631
Fragments arranged by size:
3631 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5122
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5122
NotI GC'GGCC_GC
Cuts at: 2569 2569
Size: 5122
NspI r_CATG'y
Cuts at: 1132 1229 2480 4025 1132
Size: 97 1251 1545 2229
Fragments arranged by size:
2229 1545 1251 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5122
PciI A'CATG_T
Cuts at: 4021 4021
Size: 5122
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5122
PmeI GTTT'AAAC
Cuts at: 2426 2426
Size: 5122
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 4704 4736 3191
Size: 32 1481 32 3577
Fragments arranged by size:
3577 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2243 2347 2243
Size: 104 5018
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5122
PsiI TTA'TAA
Cuts at: 2151 2692 2151
Size: 541 4581
PstI C_TGCA'G
Cuts at: 830 2474 830
Size: 1644 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5122
PvuII CAG'CTG
Cuts at: 2165 2165
Size: 5122
SalI G'TCGA_C
Cuts at: 1936 2464 1936
Size: 528 4594
SanDI GG'GwC_CC
Cuts at: 2347 2347
Size: 5122
SbfI CC_TGCA'GG
Cuts at: 2474 2474
Size: 5122
ScaI AGT'ACT
Cuts at: 3370 5082 3370
Size: 1712 3410
SfcI C'TryA_G
Cuts at: 826 1045 2470 3605 4286 4477 826
Size: 219 1425 1135 681 191 1471
Fragments arranged by size:
1471 1425 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5122
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5122
SmaI CCC'GGG
Cuts at: 1872 2449 1872
Size: 577 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2265 3226 4127 4389 820
Size: 197 926 322 961 901 262 1553
Fragments arranged by size:
1553 961 926 901 322 262 197
SnaBI TAC'GTA
Cuts at: 493 2913 493
Size: 2420 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5122
SphI G_CATG'C
Cuts at: 1132 2480 1132
Size: 1348 3774
SspI AAT'ATT
Cuts at: 5 52 3046 5
Size: 47 2994 2081
Fragments arranged by size:
2994 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5122
StyI C'CwwG_G
Cuts at: 513 1065 2004 2091 2561 513
Size: 552 939 87 470 3074
Fragments arranged by size:
3074 939 552 470 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 3149 3166 3319 3504 3949 325
Size: 1255 417 1152 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1152 445 417 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2413 3368 5080
Size: 274 80 33 51 1443 436 955 1712
Cuts at: 5080 96
Size: 138
Fragments arranged by size:
1712 1443 955 436 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5082 5114 5082
Size: 32 5090
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5122
XbaI T'CTAG_A
Cuts at: 2045 2458 2045
Size: 413 4709
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2340 2340
Size: 5122
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5122
XmaI C'CCGG_G
Cuts at: 1870 2447 1870
Size: 577 4545
XmnI GAAnn'nnTTC
Cuts at: 3251 3251
Size: 5122
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2930 276
Size: 53 83 186 936 1396 2468
Fragments arranged by size:
2468 1396 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI