Restriction map for
pFN21AB8740
(Circular) (Six-base) MAPSORT of: pFN21AB8740.seq Check: 1310 from: 1 to: 5254
pFN21AB8740 5254 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2463 2463
Size: 5254
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3064 278
Size: 53 83 186 936 1528 2468
Fragments arranged by size:
2468 1528 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2597 1937
Size: 660 4594
Acc65I G'GTAC_C
Cuts at: 2575 2575
Size: 5254
AclI AA'CG_TT
Cuts at: 1563 3381 3754 1563
Size: 1818 373 3063
Fragments arranged by size:
3063 1818 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3317 4701 4969 1632
Size: 1685 1384 268 1917
Fragments arranged by size:
1917 1685 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5057
AflIII A'CryG_T
Cuts at: 1203 2262 4153 5130 1203
Size: 1059 1891 977 1327
Fragments arranged by size:
1891 1327 1059 977
AgeI A'CCGG_T
Cuts at: 4085 4085
Size: 5254
AhdI GACnn_n'nnGTC
Cuts at: 3983 3983
Size: 5254
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5254
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5254
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2426 2427 2458 2459 2426
Size: 1 31 1 5221
Fragments arranged by size:
5221 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 2364 2997 4569 2364
Size: 633 1572 3049
Fragments arranged by size:
3049 1572 633
ApaLI G'TGCA_C
Cuts at: 3311 4467 3311
Size: 1156 4098
ApoI r'AATT_y
Cuts at: 1493 2563 2789 2941 5050 1493
Size: 1070 226 152 2109 1697
Fragments arranged by size:
2109 1697 1070 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3808 160
Size: 872 2776 1606
Fragments arranged by size:
2776 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5254
AvaI C'yCGr_G
Cuts at: 1870 1943 2141 2221 2256 2579 1870
Size: 73 198 80 35 323 4545
Fragments arranged by size:
4545 323 198 80 73 35
BamHI G'GATC_C
Cuts at: 2584 2951 2584
Size: 367 4887
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2104 2575 4030 618
Size: 325 217 627 285 32 471 1455 1842
Fragments arranged by size:
1842 1455 627 471 325 285 217 32
BanII G_rGCy'C
Cuts at: 1391 2573 1391
Size: 1182 4072
BbeI G_GCGC'C
Cuts at: 2076 2076
Size: 5254
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2433 928
Size: 971 534 3749
Fragments arranged by size:
3749 971 534
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2227 2261 2287 2321 3445 3479 1903
Size: 34 290 34 26 34 1124 34 3678
Fragments arranged by size:
3678 1124 290 34 34 34 34 26
BciVI GTATCCnnnnn_n'
Cuts at: 3146 4356 3146
Size: 1210 4044
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5254
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3865 136
Size: 107 122 71 631 605 2193 1525
Fragments arranged by size:
2193 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5249 5249
Size: 5254
BlpI GC'TnA_GC
Cuts at: 2671 2671
Size: 5254
Bme1580I G_kGCm'C
Cuts at: 1790 2109 2130 3315 4471 1790
Size: 319 21 1185 1156 2573
Fragments arranged by size:
2573 1185 1156 319 21
BmgBI CAC'GTC
Cuts at: 1318 2125 1318
Size: 807 4447
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2204 2985 3943
Size: 416 53 83 399 24 787 781 958
Cuts at: 3943 442
Size: 1753
Fragments arranged by size:
1753 958 787 781 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 3914 1239
Size: 974 1701 2579
Fragments arranged by size:
2579 1701 974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2270 3379 4244 4542 2270
Size: 1109 865 298 2982
Fragments arranged by size:
2982 1109 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3917 882
Size: 617 2418 2219
Fragments arranged by size:
2418 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2278 3045 493
Size: 711 1074 767 2702
Fragments arranged by size:
2702 1074 767 711
BsaBI GATnn'nnATC
Cuts at: 2012 5248 2012
Size: 3236 2018
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 2415 3061 3443
Size: 53 83 186 936 540 342 646 382
Cuts at: 3443 4078 275
Size: 635 1451
Fragments arranged by size:
1451 936 646 635 540 382 342 186
83 53
BsaWI w'CCGG_w
Cuts at: 3687 4085 4359 4506 4917 3687
Size: 398 274 147 411 4024
Fragments arranged by size:
4024 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5029
Fragments arranged by size:
5029 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 2366 1186
Size: 1024 156 4074
Fragments arranged by size:
4074 1024 156
BseYI C'CCAG_C
Cuts at: 2108 2342 4457 4953 2108
Size: 234 2115 496 2409
Fragments arranged by size:
2409 2115 496 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2306 2450 1163
Size: 573 570 144 3967
Fragments arranged by size:
3967 573 570 144
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2704 3465 4493 1540
Size: 275 186 703 761 1028 2301
Fragments arranged by size:
2301 1028 761 703 275 186
BsiHKAI G_wGCw'C
Cuts at: 2478 3315 3400 4471 2478
Size: 837 85 1071 3261
Fragments arranged by size:
3261 1071 837 85
BsmI GAATG_Cn'
Cuts at: 2765 2858 2765
Size: 93 5161
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2130 2478 2573 3315 3400 4471
Size: 399 319 21 348 95 742 85 1071
Cuts at: 4471 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 348 319 95 85
21
BspEI T'CCGG_A
Cuts at: 4917 4917
Size: 5254
BspHI T'CATG_A
Cuts at: 3143 4873 3143
Size: 1730 3524
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2463 2609 844
Size: 1619 146 3489
Fragments arranged by size:
3489 1619 146
BsrBI CCG'CTC
Cuts at: 1394 1823 3141 1394
Size: 429 1318 3507
Fragments arranged by size:
3507 1318 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3749 3923 65
Size: 1183 2501 174 1396
Fragments arranged by size:
2501 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2338 2417 3898 4085 1700
Size: 110 528 79 1481 187 2869
Fragments arranged by size:
2869 1481 528 187 110 79
BsrGI T'GTAC_A
Cuts at: 96 2265 96
Size: 2169 3085
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5254
BssSI C'ACGA_G
Cuts at: 3314 4326 3314
Size: 1012 4242
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2473 794
Size: 1679 3575
BstBI TT'CG_AA
Cuts at: 4058 4058
Size: 5254
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5254
BstYI r'GATC_y
Cuts at: 1972 2584 2951 3336 3353 4805 4891 4903 5249
Size: 612 367 385 17 1452 86 12 346
Cuts at: 5249 1972
Size: 1977
Fragments arranged by size:
1977 1452 612 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4702
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4515
Fragments arranged by size:
4515 683 56
BtsI GCAGTG_nn'
Cuts at: 2185 2365 2770 3564 3584 2185
Size: 180 405 794 20 3855
Fragments arranged by size:
3855 794 405 180 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5254
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5219
DraI TTT'AAA
Cuts at: 2558 2558
Size: 5254
DraIII CAC_nnn'GTG
Cuts at: 2278 2473 2278
Size: 195 5059
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4261 809
Size: 507 2945 1802
Fragments arranged by size:
2945 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2701 3590 5219
Size: 54 1425 50 275 18 871 889 1629
Cuts at: 5219 8
Size: 43
Fragments arranged by size:
1629 1425 889 871 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2701 1537
Size: 275 889 4090
Fragments arranged by size:
4090 889 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3184 1353
Size: 1831 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3841 4215 4361 387
Size: 1064 138 2252 374 146 1280
Fragments arranged by size:
2252 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 3317 3914 4701 4969 1239
Size: 393 581 1104 597 787 268 1524
Fragments arranged by size:
1524 1104 787 597 581 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2317 2317
Size: 5254
EcoRI G'AATT_C
Cuts at: 2563 2941 2563
Size: 378 4876
FspI TGC'GCA
Cuts at: 3760 3760
Size: 5254
HaeII r_GCGC'y
Cuts at: 1678 2076 2232 4401 1678
Size: 398 156 2169 2531
Fragments arranged by size:
2531 2169 398 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3895 3927 3969 4001 1373
Size: 32 135 32 2323 32 42 32 2626
Fragments arranged by size:
2626 2323 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2598 2844 669
Size: 988 281 660 246 3079
Fragments arranged by size:
3079 988 660 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5254
HpaI GTT'AAC
Cuts at: 2844 2844
Size: 5254
KasI G'GCGC_C
Cuts at: 2072 2072
Size: 5254
KpnI G_GTAC'C
Cuts at: 2579 2579
Size: 5254
MfeI C'AATT_G
Cuts at: 2542 2853 2542
Size: 311 4943
MluI A'CGCG_T
Cuts at: 5130 5130
Size: 5254
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4368 4552 1305
Size: 3063 184 2007
Fragments arranged by size:
3063 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3432
Fragments arranged by size:
3432 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 3212 3571 3730 518
Size: 648 1218 828 359 159 2042
Fragments arranged by size:
2042 1218 828 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2670 3347 4495 4740 1606
Size: 1064 677 1148 245 2120
Fragments arranged by size:
2120 1148 1064 677 245
NaeI GCC'GGC
Cuts at: 2419 2419
Size: 5254
NarI GG'CG_CC
Cuts at: 2073 2073
Size: 5254
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4702
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5254
NgoMIV G'CCGG_C
Cuts at: 2417 2417
Size: 5254
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5254
NotI GC'GGCC_GC
Cuts at: 2701 2701
Size: 5254
NspI r_CATG'y
Cuts at: 1132 1229 2266 2612 4157 1132
Size: 97 1037 346 1545 2229
Fragments arranged by size:
2229 1545 1037 346 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5254
PciI A'CATG_T
Cuts at: 2262 4153 2262
Size: 1891 3363
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2342 1108
Size: 1234 4020
PmeI GTTT'AAAC
Cuts at: 2558 2558
Size: 5254
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3323 3355 4836 4868 3323
Size: 32 1481 32 3709
Fragments arranged by size:
3709 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2317 2317
Size: 5254
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5254
PsiI TTA'TAA
Cuts at: 2824 2824
Size: 5254
PstI C_TGCA'G
Cuts at: 830 2606 830
Size: 1776 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5254
SalI G'TCGA_C
Cuts at: 1936 2596 1936
Size: 660 4594
SbfI CC_TGCA'GG
Cuts at: 2606 2606
Size: 5254
ScaI AGT'ACT
Cuts at: 3502 5214 3502
Size: 1712 3542
SfcI C'TryA_G
Cuts at: 826 1045 2602 3737 4418 4609 826
Size: 219 1557 1135 681 191 1471
Fragments arranged by size:
1557 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2074 2074
Size: 5254
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5254
SgrAI Cr'CCGG_yG
Cuts at: 1700 2417 1700
Size: 717 4537
SmaI CCC'GGG
Cuts at: 1872 2143 2581 1872
Size: 271 438 4545
Fragments arranged by size:
4545 438 271
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2285 3358 4259 4521 820
Size: 197 926 342 1073 901 262 1553
Fragments arranged by size:
1553 1073 926 901 342 262 197
SnaBI TAC'GTA
Cuts at: 493 3045 493
Size: 2552 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5254
SphI G_CATG'C
Cuts at: 1132 2612 1132
Size: 1480 3774
SspI AAT'ATT
Cuts at: 5 52 3178 5
Size: 47 3126 2081
Fragments arranged by size:
3126 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5254
StyI C'CwwG_G
Cuts at: 513 1065 2373 2693 513
Size: 552 1308 320 3074
Fragments arranged by size:
3074 1308 552 320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3281 3298 3451 3636 4081 325
Size: 1255 1701 17 153 185 445 1498
Fragments arranged by size:
1701 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2265 3500 5212
Size: 274 80 33 51 1443 288 1235 1712
Cuts at: 5212 96
Size: 138
Fragments arranged by size:
1712 1443 1235 288 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5214 5246 5214
Size: 32 5222
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5254
XbaI T'CTAG_A
Cuts at: 2590 2590
Size: 5254
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2380 2380
Size: 5254
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5254
XmaI C'CCGG_G
Cuts at: 1870 2141 2579 1870
Size: 271 438 4545
Fragments arranged by size:
4545 438 271
XmnI GAAnn'nnTTC
Cuts at: 2063 3383 2063
Size: 1320 3934
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3062 276
Size: 53 83 186 936 1528 2468
Fragments arranged by size:
2468 1528 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI