Restriction map for
pFN21AB8739
(Circular) (Six-base) MAPSORT of: pFN21AB8739.seq Check: 5596 from: 1 to: 4861
pFN21AB8739 4861 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2671 278
Size: 53 83 186 936 1135 2468
Fragments arranged by size:
2468 1135 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2204 1937
Size: 267 4594
Acc65I G'GTAC_C
Cuts at: 2182 2182
Size: 4861
AclI AA'CG_TT
Cuts at: 1563 2988 3361 1563
Size: 1425 373 3063
Fragments arranged by size:
3063 1425 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2924 4308 4576 1632
Size: 1292 1384 268 1917
Fragments arranged by size:
1917 1384 1292 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4664
AflIII A'CryG_T
Cuts at: 1203 3760 4737 1203
Size: 2557 977 1327
Fragments arranged by size:
2557 1327 977
AgeI A'CCGG_T
Cuts at: 3692 3692
Size: 4861
AhdI GACnn_n'nnGTC
Cuts at: 3590 3590
Size: 4861
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4861
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4861
AlwNI CAG_nnn'CTG
Cuts at: 2604 4176 2604
Size: 1572 3289
ApaLI G'TGCA_C
Cuts at: 2918 4074 2918
Size: 1156 3705
ApoI r'AATT_y
Cuts at: 1493 2170 2396 2548 4657 1493
Size: 677 226 152 2109 1697
Fragments arranged by size:
2109 1697 677 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3415 160
Size: 872 2383 1606
Fragments arranged by size:
2383 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4861
AvaI C'yCGr_G
Cuts at: 1870 1943 2186 1870
Size: 73 243 4545
Fragments arranged by size:
4545 243 73
BamHI G'GATC_C
Cuts at: 2191 2558 2191
Size: 367 4494
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2182 3637 618
Size: 325 217 627 395 1455 1842
Fragments arranged by size:
1842 1455 627 395 325 217
BanII G_rGCy'C
Cuts at: 1391 2180 1391
Size: 789 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3890
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3052 3086 1903
Size: 34 1115 34 3678
Fragments arranged by size:
3678 1115 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2753 3963 2753
Size: 1210 3651
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4861
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3472 136
Size: 107 122 71 631 605 1800 1525
Fragments arranged by size:
1800 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4856 4856
Size: 4861
BlpI GC'TnA_GC
Cuts at: 2032 2278 2032
Size: 246 4615
Bme1580I G_kGCm'C
Cuts at: 1790 2922 4078 1790
Size: 1132 1156 2573
Fragments arranged by size:
2573 1156 1132
BmgBI CAC'GTC
Cuts at: 1318 2078 1318
Size: 760 4101
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2592 3550 442
Size: 416 53 83 399 24 1175 958 1753
Fragments arranged by size:
1753 1175 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4861
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3521 1239
Size: 2282 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2986 3851 4149 2986
Size: 865 298 3698
Fragments arranged by size:
3698 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3524 882
Size: 617 2025 2219
Fragments arranged by size:
2219 2025 617
BsaAI yAC'GTr
Cuts at: 493 1204 2652 493
Size: 711 1448 2702
Fragments arranged by size:
2702 1448 711
BsaBI GATnn'nnATC
Cuts at: 2141 4855 2141
Size: 2714 2147
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2668 3050 3685 275
Size: 53 83 186 936 1135 382 635 1451
Fragments arranged by size:
1451 1135 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3294 3692 3966 4113 4524 3294
Size: 398 274 147 411 3631
Fragments arranged by size:
3631 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4636
Fragments arranged by size:
4636 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4861
BseYI C'CCAG_C
Cuts at: 4064 4560 4064
Size: 496 4365
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4288
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2311 3072 4100 1540
Size: 275 186 310 761 1028 2301
Fragments arranged by size:
2301 1028 761 310 275 186
BsiHKAI G_wGCw'C
Cuts at: 2922 3007 4078 2922
Size: 85 1071 3705
Fragments arranged by size:
3705 1071 85
BsmI GAATG_Cn'
Cuts at: 2372 2465 2372
Size: 93 4768
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2180 2922 3007 4078 1391
Size: 399 390 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 85
BspEI T'CCGG_A
Cuts at: 4524 4524
Size: 4861
BspHI T'CATG_A
Cuts at: 2750 4480 2750
Size: 1730 3131
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2216 844
Size: 1372 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2748 1394
Size: 429 925 3507
Fragments arranged by size:
3507 925 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3356 3530 65
Size: 1183 2108 174 1396
Fragments arranged by size:
2108 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3505 3692 1700
Size: 110 1695 187 2869
Fragments arranged by size:
2869 1695 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4861
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4861
BssSI C'ACGA_G
Cuts at: 2921 3933 2921
Size: 1012 3849
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4861
BstBI TT'CG_AA
Cuts at: 3665 3665
Size: 4861
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4861
BstYI r'GATC_y
Cuts at: 1972 2191 2558 2943 2960 4412 4498 4510 4856
Size: 219 367 385 17 1452 86 12 346
Cuts at: 4856 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 219 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3370
Fragments arranged by size:
3370 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4122
Fragments arranged by size:
4122 683 56
BtsI GCAGTG_nn'
Cuts at: 2377 3171 3191 2377
Size: 794 20 4047
Fragments arranged by size:
4047 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4861
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4826
DraI TTT'AAA
Cuts at: 2165 2165
Size: 4861
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3868 809
Size: 507 2552 1802
Fragments arranged by size:
2552 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2308 3197 4826
Size: 54 1425 50 275 18 478 889 1629
Cuts at: 4826 8
Size: 43
Fragments arranged by size:
1629 1425 889 478 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2308 1537
Size: 275 496 4090
Fragments arranged by size:
4090 496 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2038 2791 1353
Size: 685 753 3423
Fragments arranged by size:
3423 753 685
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3448 3822 3968 387
Size: 1064 138 1859 374 146 1280
Fragments arranged by size:
1859 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2924 3521 4308 4576 1239
Size: 393 1292 597 787 268 1524
Fragments arranged by size:
1524 1292 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2170 2548 2170
Size: 378 4483
FspI TGC'GCA
Cuts at: 3367 3367
Size: 4861
HaeII r_GCGC'y
Cuts at: 1678 4008 1678
Size: 2330 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3502 3534 3576 3608 1373
Size: 32 135 32 1930 32 42 32 2626
Fragments arranged by size:
2626 1930 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2205 2451 669
Size: 988 281 267 246 3079
Fragments arranged by size:
3079 988 281 267 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4861
HpaI GTT'AAC
Cuts at: 2451 2451
Size: 4861
KpnI G_GTAC'C
Cuts at: 2186 2186
Size: 4861
MfeI C'AATT_G
Cuts at: 2460 2460
Size: 4861
MluI A'CGCG_T
Cuts at: 4737 4737
Size: 4861
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3975 4159 1305
Size: 2670 184 2007
Fragments arranged by size:
2670 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3039
Fragments arranged by size:
3039 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2150 2819 3178 3337 518
Size: 648 894 90 669 359 159 2042
Fragments arranged by size:
2042 894 669 648 359 159 90
MspA1I CmG'CkG
Cuts at: 1606 2277 2954 4102 4347 1606
Size: 671 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 671 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3370
Fragments arranged by size:
3370 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4861
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4861
NotI GC'GGCC_GC
Cuts at: 2308 2308
Size: 4861
NspI r_CATG'y
Cuts at: 1132 1229 2219 3764 1132
Size: 97 990 1545 2229
Fragments arranged by size:
2229 1545 990 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4861
PciI A'CATG_T
Cuts at: 3760 3760
Size: 4861
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4861
PmeI GTTT'AAAC
Cuts at: 2165 2165
Size: 4861
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 4443 4475 2930
Size: 32 1481 32 3316
Fragments arranged by size:
3316 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4861
PsiI TTA'TAA
Cuts at: 2431 2431
Size: 4861
PstI C_TGCA'G
Cuts at: 830 2213 830
Size: 1383 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4861
SalI G'TCGA_C
Cuts at: 1936 2203 1936
Size: 267 4594
SapI GCTCTTCn'nnn_
Cuts at: 2038 2038
Size: 4861
SbfI CC_TGCA'GG
Cuts at: 2213 2213
Size: 4861
ScaI AGT'ACT
Cuts at: 3109 4821 3109
Size: 1712 3149
SfcI C'TryA_G
Cuts at: 826 1045 2087 2209 3344 4025 4216 826
Size: 219 1042 122 1135 681 191 1471
Fragments arranged by size:
1471 1135 1042 681 219 191 122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4861
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4861
SmaI CCC'GGG
Cuts at: 1872 2188 1872
Size: 316 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2965 3866 4128 820
Size: 197 926 1022 901 262 1553
Fragments arranged by size:
1553 1022 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2652 493
Size: 2159 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4861
SphI G_CATG'C
Cuts at: 1132 2219 1132
Size: 1087 3774
SspI AAT'ATT
Cuts at: 5 52 2785 5
Size: 47 2733 2081
Fragments arranged by size:
2733 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4861
StyI C'CwwG_G
Cuts at: 513 1065 2004 2300 513
Size: 552 939 296 3074
Fragments arranged by size:
3074 939 552 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2888 2905 3058 3243 3688 325
Size: 1255 1308 17 153 185 445 1498
Fragments arranged by size:
1498 1308 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3107 4819 96
Size: 274 80 33 51 1443 1130 1712 138
Fragments arranged by size:
1712 1443 1130 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4821 4853 4821
Size: 32 4829
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4861
XbaI T'CTAG_A
Cuts at: 2197 2197
Size: 4861
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4861
XmaI C'CCGG_G
Cuts at: 1870 2186 1870
Size: 316 4545
XmnI GAAnn'nnTTC
Cuts at: 2990 2990
Size: 4861
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2669 276
Size: 53 83 186 936 1135 2468
Fragments arranged by size:
2468 1135 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI