Restriction map for
pFN21AB8733
(Circular) (Six-base) MAPSORT of: pFN21AB8733.seq Check: 3741 from: 1 to: 6169
pFN21AB8733 6169 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2620 2620
Size: 6169
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3979 278
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3088 3512 1937
Size: 1151 424 4594
Fragments arranged by size:
4594 1151 424
Acc65I G'GTAC_C
Cuts at: 3490 3490
Size: 6169
AclI AA'CG_TT
Cuts at: 1563 4296 4669 1563
Size: 2733 373 3063
Fragments arranged by size:
3063 2733 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2132 2823 2851 4232 5616 5884 1632
Size: 500 691 28 1381 1384 268 1917
Fragments arranged by size:
1917 1384 1381 691 500 268 28
AflII C'TTAA_G
Cuts at: 820 1017 2780 820
Size: 197 1763 4209
Fragments arranged by size:
4209 1763 197
AflIII A'CryG_T
Cuts at: 1203 5068 6045 1203
Size: 3865 977 1327
Fragments arranged by size:
3865 1327 977
AgeI A'CCGG_T
Cuts at: 5000 5000
Size: 6169
AhdI GACnn_n'nnGTC
Cuts at: 4898 4898
Size: 6169
AleI CACnn'nnGTG
Cuts at: 1166 2423 1166
Size: 1257 4912
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6169
AlwNI CAG_nnn'CTG
Cuts at: 2497 3912 5484 2497
Size: 1415 1572 3182
Fragments arranged by size:
3182 1572 1415
ApaI G_GGCC'C
Cuts at: 2416 2416
Size: 6169
ApaLI G'TGCA_C
Cuts at: 4226 5382 4226
Size: 1156 5013
ApoI r'AATT_y
Cuts at: 1493 3478 3704 3856 5965 1493
Size: 1985 226 152 2109 1697
Fragments arranged by size:
2109 1985 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4723 160
Size: 872 3691 1606
Fragments arranged by size:
3691 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
AvaI C'yCGr_G
Cuts at: 1870 1943 3494 1870
Size: 73 1551 4545
Fragments arranged by size:
4545 1551 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2826 2859 2826
Size: 33 6136
BamHI G'GATC_C
Cuts at: 3499 3866 3499
Size: 367 5802
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3490 4945 618
Size: 325 217 627 1703 1455 1842
Fragments arranged by size:
1842 1703 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2416 2484 2550 3488 1391
Size: 1025 68 66 938 4072
Fragments arranged by size:
4072 1025 938 68 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2828 928
Size: 971 929 4269
Fragments arranged by size:
4269 971 929
BbvCI CC'TCA_GC
Cuts at: 2345 2345
Size: 6169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4360 4394 1903
Size: 34 2423 34 3678
Fragments arranged by size:
3678 2423 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2854 4061 5271 2854
Size: 1207 1210 3752
Fragments arranged by size:
3752 1210 1207
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6169
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2732 3165 4780
Size: 107 122 71 631 605 1060 433 1615
Cuts at: 4780 136
Size: 1525
Fragments arranged by size:
1615 1525 1060 631 605 433 122 107
71
BglII A'GATC_T
Cuts at: 2580 6164 2580
Size: 3584 2585
BlpI GC'TnA_GC
Cuts at: 3586 3586
Size: 6169
Bme1580I G_kGCm'C
Cuts at: 1790 2142 2416 4230 5386 1790
Size: 352 274 1814 1156 2573
Fragments arranged by size:
2573 1814 1156 352 274
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6169
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3106 3136 3900
Size: 416 53 83 399 24 1689 30 764
Cuts at: 3900 4858 442
Size: 958 1753
Fragments arranged by size:
1753 1689 958 764 416 399 83 53
30 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2270 2498 4829 1239
Size: 884 147 228 2331 2579
Fragments arranged by size:
2579 2331 884 228 147
Bpu10I CC'TnA_GC
Cuts at: 2345 2345
Size: 6169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2534 2605 4294 5159 5457 2534
Size: 71 1689 865 298 3246
Fragments arranged by size:
3246 1689 865 298 71
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4832 882
Size: 617 3333 2219
Fragments arranged by size:
3333 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3960 493
Size: 711 2756 2702
Fragments arranged by size:
2756 2702 711
BsaBI GATnn'nnATC
Cuts at: 6163 6163
Size: 6169
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3976 4358 4993 275
Size: 53 83 186 936 2443 382 635 1451
Fragments arranged by size:
2443 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4602 5000 5274 5421 5832 4602
Size: 398 274 147 411 4939
Fragments arranged by size:
4939 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2738 2768 1178
Size: 30 165 30 1335 30 4579
Fragments arranged by size:
4579 1335 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2543 1186
Size: 1357 4812
BseYI C'CCAG_C
Cuts at: 2030 5372 5868 2030
Size: 3342 496 2331
Fragments arranged by size:
3342 2331 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3417 1163
Size: 573 1681 3915
Fragments arranged by size:
3915 1681 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3619 4380 5408 1540
Size: 275 186 1618 761 1028 2301
Fragments arranged by size:
2301 1618 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2550 4230 4315 5386 2550
Size: 1680 85 1071 3333
Fragments arranged by size:
3333 1680 1071 85
BsmI GAATG_Cn'
Cuts at: 3433 3680 3773 3433
Size: 247 93 5829
Fragments arranged by size:
5829 247 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2416 2484 2550 3488 4230 4315
Size: 399 352 274 68 66 938 742 85
Cuts at: 4315 5386 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 938 742 399 352 274 85
68 66
BspEI T'CCGG_A
Cuts at: 5832 5832
Size: 6169
BspHI T'CATG_A
Cuts at: 4058 5788 4058
Size: 1730 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2620 3524 844
Size: 1776 904 3489
Fragments arranged by size:
3489 1776 904
BsrBI CCG'CTC
Cuts at: 1394 1823 3345 4056 1394
Size: 429 1522 711 3507
Fragments arranged by size:
3507 1522 711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4664 4838 65
Size: 1183 3416 174 1396
Fragments arranged by size:
3416 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3311 4813 5000 1700
Size: 110 1501 1502 187 2869
Fragments arranged by size:
2869 1502 1501 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6169
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6169
BssSI C'ACGA_G
Cuts at: 4229 5241 4229
Size: 1012 5157
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2157 2635 794
Size: 1363 478 4328
Fragments arranged by size:
4328 1363 478
BstBI TT'CG_AA
Cuts at: 4973 4973
Size: 6169
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6169
BstXI CCAn_nnnn'nTGG
Cuts at: 2854 3462 2854
Size: 608 5561
BstYI r'GATC_y
Cuts at: 1972 2304 2580 3016 3499 3866 4251 4268 5720
Size: 332 276 436 483 367 385 17 1452
Cuts at: 5720 5806 5818 6164 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 483 436 385 367 346 332
276 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2897 3082 2897
Size: 185 5984
BtgI C'CryG_G
Cuts at: 513 1065 2004 2826 513
Size: 552 939 822 3856
Fragments arranged by size:
3856 939 822 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5430
Fragments arranged by size:
5430 683 56
BtsI GCAGTG_nn'
Cuts at: 3138 3685 4479 4499 3138
Size: 547 794 20 4808
Fragments arranged by size:
4808 794 547 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6134
DraI TTT'AAA
Cuts at: 3473 3473
Size: 6169
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5176 809
Size: 507 3860 1802
Fragments arranged by size:
3860 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3616 4505 6134
Size: 54 1425 50 275 18 1786 889 1629
Cuts at: 6134 8
Size: 43
Fragments arranged by size:
1786 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3616 1537
Size: 275 1804 4090
Fragments arranged by size:
4090 1804 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2592 2616 2649 3401 4099 1353
Size: 1239 24 33 752 698 3423
Fragments arranged by size:
3423 1239 752 698 33 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4756 5130 5276 387
Size: 1064 138 3167 374 146 1280
Fragments arranged by size:
3167 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2548 2548
Size: 6169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2132 2270 2498 2823 2851 4232
Size: 393 491 9 138 228 325 28 1381
Cuts at: 4232 4829 5616 5884 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1381 787 597 491 393 325 268
228 138 28 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2972 2972
Size: 6169
EcoO109I rG'GnC_Cy
Cuts at: 2034 2179 2341 2719 3450 2034
Size: 145 162 378 731 4753
Fragments arranged by size:
4753 731 378 162 145
EcoRI G'AATT_C
Cuts at: 3478 3856 3478
Size: 378 5791
FspI TGC'GCA
Cuts at: 4675 4675
Size: 6169
HaeII r_GCGC'y
Cuts at: 1678 2716 5316 1678
Size: 1038 2600 2531
Fragments arranged by size:
2600 2531 1038
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2565 2597 2938 2970 4810
Size: 32 135 32 993 32 341 32 1840
Cuts at: 4810 4842 4884 4916 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1840 993 341 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3089 3174 3513 3759 669
Size: 988 281 1151 85 339 246 3079
Fragments arranged by size:
3079 1151 988 339 281 246 85
HindIII A'AGCT_T
Cuts at: 748 2154 748
Size: 1406 4763
HpaI GTT'AAC
Cuts at: 3759 3759
Size: 6169
KpnI G_GTAC'C
Cuts at: 3494 3494
Size: 6169
MfeI C'AATT_G
Cuts at: 3768 3768
Size: 6169
MluI A'CGCG_T
Cuts at: 6045 6045
Size: 6169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2263 5283 5467 1305
Size: 958 3020 184 2007
Fragments arranged by size:
3020 2007 958 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4347
Fragments arranged by size:
4347 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 2831 3269 4127 4486 4645 518
Size: 648 1257 408 438 858 359 159 2042
Fragments arranged by size:
2042 1257 858 648 438 408 359 159
MspA1I CmG'CkG
Cuts at: 1606 2054 2169 3585 4262 5410 5655 1606
Size: 448 115 1416 677 1148 245 2120
Fragments arranged by size:
2120 1416 1148 677 448 245 115
NaeI GCC'GGC
Cuts at: 3313 3313
Size: 6169
NcoI C'CATG_G
Cuts at: 513 1065 2004 2826 513
Size: 552 939 822 3856
Fragments arranged by size:
3856 939 822 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6169
NgoMIV G'CCGG_C
Cuts at: 3311 3311
Size: 6169
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6169
NotI GC'GGCC_GC
Cuts at: 3616 3616
Size: 6169
NspI r_CATG'y
Cuts at: 1132 1229 3527 5072 1132
Size: 97 2298 1545 2229
Fragments arranged by size:
2298 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6169
PciI A'CATG_T
Cuts at: 5068 5068
Size: 6169
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6169
PfoI T'CCnGG_A
Cuts at: 2307 3012 2307
Size: 705 5464
PmeI GTTT'AAAC
Cuts at: 3473 3473
Size: 6169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4238 4270 5751 5783 4238
Size: 32 1481 32 4624
Fragments arranged by size:
4624 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2179 2719 2179
Size: 540 5629
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6169
PsiI TTA'TAA
Cuts at: 3739 3739
Size: 6169
PspOMI G'GGCC_C
Cuts at: 2412 2412
Size: 6169
PstI C_TGCA'G
Cuts at: 830 2044 2469 2502 2676 3521 830
Size: 1214 425 33 174 845 3478
Fragments arranged by size:
3478 1214 845 425 174 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6169
PvuII CAG'CTG
Cuts at: 2054 2054
Size: 6169
SacI G_AGCT'C
Cuts at: 2550 2550
Size: 6169
SalI G'TCGA_C
Cuts at: 1936 3087 3511 1936
Size: 1151 424 4594
Fragments arranged by size:
4594 1151 424
SapI GCTCTTCn'nnn_
Cuts at: 2592 2616 2592
Size: 24 6145
SbfI CC_TGCA'GG
Cuts at: 3521 3521
Size: 6169
ScaI AGT'ACT
Cuts at: 2888 4417 6129 2888
Size: 1529 1712 2928
Fragments arranged by size:
2928 1712 1529
SfcI C'TryA_G
Cuts at: 826 1045 2040 2049 2465 2498 2672 3517 4652
Size: 219 995 9 416 33 174 845 1135
Cuts at: 4652 5333 5524 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 995 845 681 416 219 191
174 33 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6169
SmaI CCC'GGG
Cuts at: 1872 3496 1872
Size: 1624 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2549 2584 2780 4273 5174 5436
Size: 197 926 606 35 196 1493 901 262
Cuts at: 5436 820
Size: 1553
Fragments arranged by size:
1553 1493 926 901 606 262 197 196
35
SnaBI TAC'GTA
Cuts at: 493 3960 493
Size: 3467 2702
SpeI A'CTAG_T
Cuts at: 152 2557 152
Size: 2405 3764
SphI G_CATG'C
Cuts at: 1132 3527 1132
Size: 2395 3774
SspI AAT'ATT
Cuts at: 5 52 4093 5
Size: 47 4041 2081
Fragments arranged by size:
4041 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6169
StyI C'CwwG_G
Cuts at: 513 1065 2004 2701 2826 3032 3608 513
Size: 552 939 697 125 206 576 3074
Fragments arranged by size:
3074 939 697 576 552 206 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2420 3125 4196 4213 4366 4551 4996
Size: 1255 840 705 1071 17 153 185 445
Cuts at: 4996 325
Size: 1498
Fragments arranged by size:
1498 1255 1071 840 705 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2451 2886 4415
Size: 274 80 33 51 1443 474 435 1529
Cuts at: 4415 6127 96
Size: 1712 138
Fragments arranged by size:
1712 1529 1443 474 435 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2738 2770 6129 6161 2738
Size: 32 3359 32 2746
Fragments arranged by size:
3359 2746 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6169
XbaI T'CTAG_A
Cuts at: 3505 3505
Size: 6169
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6169
XmaI C'CCGG_G
Cuts at: 1870 3494 1870
Size: 1624 4545
XmnI GAAnn'nnTTC
Cuts at: 2601 4298 2601
Size: 1697 4472
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3977 276
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI