Restriction map for
pFN21AB8729
(Circular) (Six-base) MAPSORT of: pFN21AB8729.seq Check: 5112 from: 1 to: 7228
pFN21AB8729 7228 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2021 2267 2587 2872 3432 4356 2021
Size: 246 320 285 560 924 4893
Fragments arranged by size:
4893 924 560 320 285 246
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2609 3323 3774 5038
Size: 53 83 186 936 1073 714 451 1264
Cuts at: 5038 278
Size: 2468
Fragments arranged by size:
2468 1264 1073 936 714 451 186 83
53
AccI GT'mk_AC
Cuts at: 1937 3774 4031 4571 1937
Size: 1837 257 540 4594
Fragments arranged by size:
4594 1837 540 257
Acc65I G'GTAC_C
Cuts at: 4549 4549
Size: 7228
AclI AA'CG_TT
Cuts at: 1563 5355 5728 1563
Size: 3792 373 3063
Fragments arranged by size:
3792 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2603 3704 4028 5291 6675 6943 1632
Size: 971 1101 324 1263 1384 268 1917
Fragments arranged by size:
1917 1384 1263 1101 971 324 268
AfeI AGC'GCT
Cuts at: 4469 4469
Size: 7228
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7031
AflIII A'CryG_T
Cuts at: 1203 2871 6127 7104 1203
Size: 1668 3256 977 1327
Fragments arranged by size:
3256 1668 1327 977
AgeI A'CCGG_T
Cuts at: 6059 6059
Size: 7228
AhdI GACnn_n'nnGTC
Cuts at: 3055 4060 5957 3055
Size: 1005 1897 4326
Fragments arranged by size:
4326 1897 1005
AleI CACnn'nnGTG
Cuts at: 1166 2421 4448 1166
Size: 1255 2027 3946
Fragments arranged by size:
3946 2027 1255
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4082 1184
Size: 2898 4330
AlwNI CAG_nnn'CTG
Cuts at: 2176 2602 3511 3844 4971 6543 2176
Size: 426 909 333 1127 1572 2861
Fragments arranged by size:
2861 1572 1127 909 426 333
ApaI G_GGCC'C
Cuts at: 3284 4118 4324 3284
Size: 834 206 6188
Fragments arranged by size:
6188 834 206
ApaLI G'TGCA_C
Cuts at: 3185 5285 6441 3185
Size: 2100 1156 3972
Fragments arranged by size:
3972 2100 1156
ApoI r'AATT_y
Cuts at: 1493 3812 4537 4763 4915 7024 1493
Size: 2319 725 226 152 2109 1697
Fragments arranged by size:
2319 2109 1697 725 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5782 160
Size: 872 4750 1606
Fragments arranged by size:
4750 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7228
AvaI C'yCGr_G
Cuts at: 1870 1943 2484 2865 3143 3235 4553 1870
Size: 73 541 381 278 92 1318 4545
Fragments arranged by size:
4545 1318 541 381 278 92 73
BamHI G'GATC_C
Cuts at: 3024 3579 4558 4925 3024
Size: 555 979 367 5327
Fragments arranged by size:
5327 979 555 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2101 2456 3420 3950 4267
Size: 325 217 627 314 355 964 530 317
Cuts at: 4267 4510 4549 6004 618
Size: 243 39 1455 1842
Fragments arranged by size:
1842 1455 964 627 530 355 325 317
314 243 217 39
BanII G_rGCy'C
Cuts at: 1391 2366 3284 3731 4118 4324 4500 4547 1391
Size: 975 918 447 387 206 176 47 4072
Fragments arranged by size:
4072 975 918 447 387 206 176 47
BbeI G_GCGC'C
Cuts at: 2460 4271 2460
Size: 1811 5417
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3208 928
Size: 971 1309 4948
Fragments arranged by size:
4948 1309 971
BbvCI CC'TCA_GC
Cuts at: 3920 4517 3920
Size: 597 6631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4229 4263 5419 5453 1903
Size: 34 2292 34 1156 34 3678
Fragments arranged by size:
3678 2292 1156 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4039 5120 6330 4039
Size: 1081 1210 4937
Fragments arranged by size:
4937 1210 1081
BclI T'GATC_A
Cuts at: 1548 3792 1548
Size: 2244 4984
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2593 3593 5839
Size: 107 122 71 631 605 921 1000 2246
Cuts at: 5839 136
Size: 1525
Fragments arranged by size:
2246 1525 1000 921 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2976 3750 7223 2976
Size: 774 3473 2981
Fragments arranged by size:
3473 2981 774
BlpI GC'TnA_GC
Cuts at: 3359 4645 3359
Size: 1286 5942
Bme1580I G_kGCm'C
Cuts at: 1790 2106 3189 3284 4118 4324 4515 5289 6445
Size: 316 1083 95 834 206 191 774 1156
Cuts at: 6445 1790
Size: 2573
Fragments arranged by size:
2573 1156 1083 834 774 316 206 191
95
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7228
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3489 4959 5917
Size: 416 53 83 399 24 2072 1470 958
Cuts at: 5917 442
Size: 1753
Fragments arranged by size:
2072 1753 1470 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7228
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 7228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2347 2446 3419 3458 5888 1239
Size: 1046 62 99 973 39 2430 2579
Fragments arranged by size:
2579 2430 1046 973 99 62 39
Bpu10I CC'TnA_GC
Cuts at: 3920 4517 3920
Size: 597 6631
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2837 3092 3785 4409 5353 6218 6516 2837
Size: 255 693 624 944 865 298 3549
Fragments arranged by size:
3549 944 865 693 624 298 255
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5891 882
Size: 617 4392 2219
Fragments arranged by size:
4392 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2443 3487 5019 493
Size: 711 1239 1044 1532 2702
Fragments arranged by size:
2702 1532 1239 1044 711
BsaBI GATnn'nnATC
Cuts at: 2192 7222 2192
Size: 5030 2198
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2457 2606 3320 3771
Size: 53 83 186 936 924 149 714 451
Cuts at: 3771 4268 5035 5417 6052 275
Size: 497 767 382 635 1451
Fragments arranged by size:
1451 936 924 767 714 635 497 451
382 186 149 83 53
BsaWI w'CCGG_w
Cuts at: 5661 6059 6333 6480 6891 5661
Size: 398 274 147 411 5998
Fragments arranged by size:
5998 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2060 2090 2915 2945 1178
Size: 30 165 30 657 30 825 30 5461
Fragments arranged by size:
5461 825 657 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3545 1186
Size: 2359 4869
BseYI C'CCAG_C
Cuts at: 2355 2588 3958 4013 4117 4166 4184 6431 6927
Size: 233 1370 55 104 49 18 2247 496
Cuts at: 6927 2355
Size: 2656
Fragments arranged by size:
2656 2247 1370 496 233 104 55 49
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2207 2399 2423 2741 2788 3467 3509
Size: 573 471 192 24 318 47 679 42
Cuts at: 3509 3604 3739 4343 4381 1163
Size: 95 135 604 38 4010
Fragments arranged by size:
4010 679 604 573 471 318 192 135
95 47 42 38 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2638 3063 3739 4678 5439 6467
Size: 275 186 637 425 676 939 761 1028
Cuts at: 6467 1540
Size: 2301
Fragments arranged by size:
2301 1028 939 761 676 637 425 275
186
BsiHKAI G_wGCw'C
Cuts at: 2087 2211 2244 2366 3189 3369 5289 5374 6445
Size: 124 33 122 823 180 1920 85 1071
Cuts at: 6445 2087
Size: 2870
Fragments arranged by size:
2870 1920 1071 823 180 124 122 85
33
BsmI GAATG_Cn'
Cuts at: 4157 4391 4739 4832 4157
Size: 234 348 93 6553
Fragments arranged by size:
6553 348 234 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2106 2211 2244 2366 3189 3284
Size: 399 297 19 105 33 122 823 95
Cuts at: 3284 3369 3731 4118 4324 4500 4515 4547 5289
Size: 85 362 387 206 176 15 32 742
Cuts at: 5289 5374 6445 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 823 742 399 387 362 297
206 176 122 105 95 85 85 33
32 19 15
BspEI T'CCGG_A
Cuts at: 6891 6891
Size: 7228
BspHI T'CATG_A
Cuts at: 3789 5117 6847 3789
Size: 1328 1730 4170
Fragments arranged by size:
4170 1730 1328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2021 2163 2229 2267 2587 2872 3432 3553
Size: 1177 142 66 38 320 285 560 121
Cuts at: 3553 3894 4356 4583 844
Size: 341 462 227 3489
Fragments arranged by size:
3489 1177 560 462 341 320 285 227
142 121 66 38
BsrBI CCG'CTC
Cuts at: 1394 1823 5115 1394
Size: 429 3292 3507
Fragments arranged by size:
3507 3292 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5723 5897 65
Size: 1183 4475 174 1396
Fragments arranged by size:
4475 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2625 2666 3189 3253 3455 3738 5872
Size: 110 815 41 523 64 202 283 2134
Cuts at: 5872 6059 1700
Size: 187 2869
Fragments arranged by size:
2869 2134 815 523 283 202 187 110
64 41
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7228
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7228
BssSI C'ACGA_G
Cuts at: 2246 3527 5288 6300 2246
Size: 1281 1761 1012 3174
Fragments arranged by size:
3174 1761 1281 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2091 3844 3856 4395 4404 794
Size: 1297 1753 12 539 9 3618
Fragments arranged by size:
3618 1753 1297 539 12 9
BstBI TT'CG_AA
Cuts at: 6032 6032
Size: 7228
BstEII G'GTnAC_C
Cuts at: 1184 2566 3451 3625 1184
Size: 1382 885 174 4787
Fragments arranged by size:
4787 1382 885 174
BstXI CCAn_nnnn'nTGG
Cuts at: 3046 3046
Size: 7228
BstYI r'GATC_y
Cuts at: 1972 2778 2976 3024 3579 3750 4095 4558 4925
Size: 806 198 48 555 171 345 463 367
Cuts at: 4925 5310 5327 6779 6865 6877 7223 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 806 555 463 385 367 346
345 198 171 86 48 17 12
BstZ17I GTA'TAC
Cuts at: 4032 4032
Size: 7228
BtgI C'CryG_G
Cuts at: 513 1065 2148 2529 2691 2985 3063 3207 3228
Size: 552 1083 381 162 294 78 144 21
Cuts at: 3228 3276 3349 3384 4377 4443 513
Size: 48 73 35 993 66 3298
Fragments arranged by size:
3298 1083 993 552 381 294 162 144
78 73 66 48 35 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2122 2178 2641 3649 4122 487
Size: 683 56 896 56 463 1008 473 3593
Fragments arranged by size:
3593 1008 896 683 473 463 56 56
BtsI GCAGTG_nn'
Cuts at: 2089 3197 3833 3866 4291 4463 4744 5538 5558
Size: 1108 636 33 425 172 281 794 20
Cuts at: 5558 2089
Size: 3759
Fragments arranged by size:
3759 1108 794 636 425 281 172 33
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7228
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7193
DraI TTT'AAA
Cuts at: 4532 4532
Size: 7228
DraIII CAC_nnn'GTG
Cuts at: 3184 4404 3184
Size: 1220 6008
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3173 6235 809
Size: 507 1857 3062 1802
Fragments arranged by size:
3062 1857 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2021 2635 2649
Size: 54 1425 50 275 18 191 614 14
Cuts at: 2649 2688 3033 3060 3114 3210 3255 3585 3891
Size: 39 345 27 54 96 45 330 306
Cuts at: 3891 4035 4261 4675 5564 7193 8
Size: 144 226 414 889 1629 43
Fragments arranged by size:
1629 1425 889 614 414 345 330 306
275 226 191 144 96 54 54 50
45 43 39 27 18 14
EagI C'GGCC_G
Cuts at: 1537 1812 2635 3060 4675 1537
Size: 275 823 425 1615 4090
Fragments arranged by size:
4090 1615 823 425 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3434 3662 4076 5158 1353
Size: 2081 228 414 1082 3423
Fragments arranged by size:
3423 2081 1082 414 228
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2351 2711 3037 3359 4064 5815
Size: 1064 138 762 360 326 322 705 1751
Cuts at: 5815 6189 6335 387
Size: 374 146 1280
Fragments arranged by size:
1751 1280 1064 762 705 374 360 326
322 146 138
EcoICRI GAG'CTC
Cuts at: 2364 2364
Size: 7228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2347 2446 2603 3419 3458 3704
Size: 393 653 62 99 157 816 39 246
Cuts at: 3704 4028 5291 5888 6675 6943 1239
Size: 324 1263 597 787 268 1524
Fragments arranged by size:
1524 1263 816 787 653 597 393 324
268 246 157 99 62 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2381 2939 3560 4399 2381
Size: 558 621 839 5210
Fragments arranged by size:
5210 839 621 558
EcoO109I rG'GnC_Cy
Cuts at: 2173 2218 2389 3281 3565 4000 4114 4320 4321
Size: 45 171 892 284 435 114 206 1
Cuts at: 4321 2173
Size: 5080
Fragments arranged by size:
5080 892 435 284 206 171 114 45
1
EcoRI G'AATT_C
Cuts at: 4537 4915 4537
Size: 378 6850
FspI TGC'GCA
Cuts at: 2159 2939 5734 2159
Size: 780 2795 3653
Fragments arranged by size:
3653 2795 780
HaeII r_GCGC'y
Cuts at: 1678 2460 3071 3268 3416 3845 4142 4271 4371
Size: 782 611 197 148 429 297 129 100
Cuts at: 4371 4471 6375 1678
Size: 100 1904 2531
Fragments arranged by size:
2531 1904 782 611 429 297 197 148
129 100 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2210 2242 2915
Size: 32 135 32 488 32 118 32 673
Cuts at: 2915 2947 3041 3073 3938 3970 4046 4078 4087
Size: 32 94 32 865 32 76 32 9
Cuts at: 4087 4119 5869 5901 5943 5975 1373
Size: 32 1750 32 42 32 2626
Fragments arranged by size:
2626 1750 865 673 488 135 118 94
76 42 32 32 32 32 32 32
32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3775 4572 4818 669
Size: 988 281 1837 797 246 3079
Fragments arranged by size:
3079 1837 988 797 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7228
HpaI GTT'AAC
Cuts at: 4818 4818
Size: 7228
KasI G'GCGC_C
Cuts at: 2456 4267 2456
Size: 1811 5417
KpnI G_GTAC'C
Cuts at: 4553 4553
Size: 7228
MfeI C'AATT_G
Cuts at: 4827 4827
Size: 7228
MluI A'CGCG_T
Cuts at: 7104 7104
Size: 7228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2856 6342 6526 1305
Size: 1551 3486 184 2007
Fragments arranged by size:
3486 2007 1551 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2690 3116 3587 3893 10
Size: 54 1425 343 858 426 471 306 3345
Fragments arranged by size:
3345 1425 858 471 426 343 306 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2421 2684 4448 5186 5545 5704 518
Size: 648 1255 263 1764 738 359 159 2042
Fragments arranged by size:
2042 1764 1255 738 648 359 263 159
MspA1I CmG'CkG
Cuts at: 1606 2027 2559 2592 3230 3278 3397 3551 4017
Size: 421 532 33 638 48 119 154 466
Cuts at: 4017 4174 4644 5321 6469 6714 1606
Size: 157 470 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 638 532 470 466 421
245 157 154 119 48 33
NaeI GCC'GGC
Cuts at: 2627 3255 2627
Size: 628 6600
NarI GG'CG_CC
Cuts at: 2457 4268 2457
Size: 1811 5417
NcoI C'CATG_G
Cuts at: 513 1065 2148 2529 3207 3384 513
Size: 552 1083 381 678 177 4357
Fragments arranged by size:
4357 1083 678 552 381 177
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7228
NgoMIV G'CCGG_C
Cuts at: 2625 3253 2625
Size: 628 6600
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7228
NotI GC'GGCC_GC
Cuts at: 2635 4675 2635
Size: 2040 5188
NruI TCG'CGA
Cuts at: 4107 4107
Size: 7228
NspI r_CATG'y
Cuts at: 1132 1229 2875 3967 4063 4586 6131 1132
Size: 97 1646 1092 96 523 1545 2229
Fragments arranged by size:
2229 1646 1545 1092 523 97 96
PasI CC'CwG_GG
Cuts at: 1348 2214 1348
Size: 866 6362
PciI A'CATG_T
Cuts at: 2871 6127 2871
Size: 3256 3972
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2197 3397 4092 1108
Size: 1089 1200 695 4244
Fragments arranged by size:
4244 1200 1089 695
PfoI T'CCnGG_A
Cuts at: 2196 2196
Size: 7228
PmeI GTTT'AAAC
Cuts at: 4532 4532
Size: 7228
PmlI CAC'GTG
Cuts at: 2443 3487 2443
Size: 1044 6184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5297 5329 6810 6842 5297
Size: 32 1481 32 5683
Fragments arranged by size:
5683 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2173 2218 2389 3565 2173
Size: 45 171 1176 5836
Fragments arranged by size:
5836 1176 171 45
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7228
PsiI TTA'TAA
Cuts at: 4798 4798
Size: 7228
PspOMI G'GGCC_C
Cuts at: 3280 4114 4320 3280
Size: 834 206 6188
Fragments arranged by size:
6188 834 206
PstI C_TGCA'G
Cuts at: 830 3204 3561 3840 3849 4233 4257 4302 4580
Size: 2374 357 279 9 384 24 45 278
Cuts at: 4580 830
Size: 3478
Fragments arranged by size:
3478 2374 384 357 279 278 45 24
9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7228
PvuII CAG'CTG
Cuts at: 4017 4174 4017
Size: 157 7071
SacI G_AGCT'C
Cuts at: 2366 2366
Size: 7228
SacII CC_GC'GG
Cuts at: 3231 3279 3231
Size: 48 7180
SalI G'TCGA_C
Cuts at: 1936 3773 4570 1936
Size: 1837 797 4594
Fragments arranged by size:
4594 1837 797
SanDI GG'GwC_CC
Cuts at: 2389 2389
Size: 7228
SapI GCTCTTCn'nnn_
Cuts at: 3434 3662 4076 3434
Size: 228 414 6586
Fragments arranged by size:
6586 414 228
SbfI CC_TGCA'GG
Cuts at: 4580 4580
Size: 7228
ScaI AGT'ACT
Cuts at: 3443 5476 7188 3443
Size: 2033 1712 3483
Fragments arranged by size:
3483 2033 1712
SexAI A'CCwGG_T
Cuts at: 4400 4400
Size: 7228
SfcI C'TryA_G
Cuts at: 826 1045 3200 3220 3557 3836 3845 4229 4253
Size: 219 2155 20 337 279 9 384 24
Cuts at: 4253 4298 4576 5711 6392 6583 826
Size: 45 278 1135 681 191 1471
Fragments arranged by size:
2155 1471 1135 681 384 337 279 278
219 191 45 24 20 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 3593 3593
Size: 7228
SfoI GGC'GCC
Cuts at: 2458 4269 2458
Size: 1811 5417
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7228
SgrAI Cr'CCGG_yG
Cuts at: 1700 2666 1700
Size: 966 6262
SmaI CCC'GGG
Cuts at: 1872 2867 4555 1872
Size: 995 1688 4545
Fragments arranged by size:
4545 1688 995
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2852 3107 3800 4424 5332 6233
Size: 197 926 909 255 693 624 908 901
Cuts at: 6233 6495 820
Size: 262 1553
Fragments arranged by size:
1553 926 909 908 901 693 624 262
255 197
SnaBI TAC'GTA
Cuts at: 493 5019 493
Size: 4526 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7228
SphI G_CATG'C
Cuts at: 1132 3967 4586 1132
Size: 2835 619 3774
Fragments arranged by size:
3774 2835 619
SspI AAT'ATT
Cuts at: 5 52 5152 5
Size: 47 5100 2081
Fragments arranged by size:
5100 2081 47
StuI AGG'CCT
Cuts at: 1517 3241 3596 4394 1517
Size: 1724 355 798 4351
Fragments arranged by size:
4351 1724 798 355
StyI C'CwwG_G
Cuts at: 513 1065 2148 2529 3207 3384 4363 4667 513
Size: 552 1083 381 678 177 979 304 3074
Fragments arranged by size:
3074 1083 979 678 552 381 304 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2102 2861 3761 3853 5255 5272 5425
Size: 1255 522 759 900 92 1402 17 153
Cuts at: 5425 5610 6055 325
Size: 185 445 1498
Fragments arranged by size:
1498 1402 1255 900 759 522 445 185
153 92 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3441 3534 5474
Size: 274 80 33 51 1443 1464 93 1940
Cuts at: 5474 7186 96
Size: 1712 138
Fragments arranged by size:
1940 1712 1464 1443 274 138 93 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2940 2972 7188 7220 2940
Size: 32 4216 32 2948
Fragments arranged by size:
4216 2948 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3785 1316
Size: 2469 4759
XbaI T'CTAG_A
Cuts at: 4564 4564
Size: 7228
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2260 2917 3043 3580 4213 2260
Size: 657 126 537 633 5275
Fragments arranged by size:
5275 657 633 537 126
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7228
XmaI C'CCGG_G
Cuts at: 1870 2865 4553 1870
Size: 995 1688 4545
Fragments arranged by size:
4545 1688 995
XmnI GAAnn'nnTTC
Cuts at: 5357 5357
Size: 7228
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2607 3321 3772 5036
Size: 53 83 186 936 1073 714 451 1264
Cuts at: 5036 276
Size: 2468
Fragments arranged by size:
2468 1264 1073 936 714 451 186 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BsmBI Bsu36I
EcoRV FalI FseI FspAI NsiI PacI PspXI PsrI
RsrII SrfI SwaI