Restriction map for
pFN21AB8728
(Circular) (Six-base) MAPSORT of: pFN21AB8728.seq Check: 3839 from: 1 to: 5821
pFN21AB8728 5821 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2507 2967 3164 1937
Size: 570 460 197 4594
Fragments arranged by size:
4594 570 460 197
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2642 3884 5268 5536 1632
Size: 1010 1242 1384 268 1917
Fragments arranged by size:
1917 1384 1242 1010 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 2474 1166
Size: 1308 4513
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3102 1184
Size: 1918 3903
AlwNI CAG_nnn'CTG
Cuts at: 2145 2503 2758 3564 5136 2145
Size: 358 255 806 1572 2830
Fragments arranged by size:
2830 1572 806 358 255
ApaLI G'TGCA_C
Cuts at: 2477 3878 5034 2477
Size: 1401 1156 3264
Fragments arranged by size:
3264 1401 1156
ApoI r'AATT_y
Cuts at: 1493 2791 3130 3356 3508 5617 1493
Size: 1298 339 226 152 2109 1697
Fragments arranged by size:
2109 1697 1298 339 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 3146 1870
Size: 73 1203 4545
Fragments arranged by size:
4545 1203 73
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2266 3142 4597 618
Size: 325 217 627 479 876 1455 1842
Fragments arranged by size:
1842 1455 876 627 479 325 217
BanII G_rGCy'C
Cuts at: 1391 2025 3140 1391
Size: 634 1115 4072
Fragments arranged by size:
4072 1115 634
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2106 2630 2717 3045 928
Size: 971 207 524 87 328 3704
Fragments arranged by size:
3704 971 524 328 207 87
BbvCI CC'TCA_GC
Cuts at: 2886 2886
Size: 5821
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4432 136
Size: 107 122 71 631 605 2760 1525
Fragments arranged by size:
2760 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2077 2808 5816 2077
Size: 731 3008 2082
Fragments arranged by size:
3008 2082 731
BlpI GC'TnA_GC
Cuts at: 3238 3238
Size: 5821
Bme1580I G_kGCm'C
Cuts at: 1790 2271 2403 2481 3882 5038 1790
Size: 481 132 78 1401 1156 2573
Fragments arranged by size:
2573 1401 1156 481 132 78
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3552 4510 442
Size: 416 53 83 399 24 2135 958 1753
Fragments arranged by size:
2135 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 2387 2651 2919 4481 1239
Size: 1106 42 264 268 1562 2579
Fragments arranged by size:
2579 1562 1106 268 264 42
Bpu10I CC'TnA_GC
Cuts at: 2886 2886
Size: 5821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3946 4811 5109 3946
Size: 865 298 4658
Fragments arranged by size:
4658 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4484 882
Size: 617 2985 2219
Fragments arranged by size:
2985 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2935 3612 493
Size: 711 1731 677 2702
Fragments arranged by size:
2702 1731 711 677
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2637 3628 4010 4645
Size: 53 83 186 936 1104 991 382 635
Cuts at: 4645 275
Size: 1451
Fragments arranged by size:
1451 1104 991 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2951 2973 4254 4652 4926 5073 5484 2951
Size: 22 1281 398 274 147 411 3288
Fragments arranged by size:
3288 1281 411 398 274 147 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5596
Fragments arranged by size:
5596 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2305 2903 1186
Size: 1119 598 4104
Fragments arranged by size:
4104 1119 598
BseYI C'CCAG_C
Cuts at: 5024 5520 5024
Size: 496 5325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2627 1163
Size: 573 891 4357
Fragments arranged by size:
4357 891 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2481 3882 3967 5038 2481
Size: 1401 85 1071 3264
Fragments arranged by size:
3264 1401 1071 85
BsmI GAATG_Cn'
Cuts at: 2983 3332 3425 2983
Size: 349 93 5379
Fragments arranged by size:
5379 349 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2271 2403 2481 3140 3882 3967
Size: 399 235 246 132 78 659 742 85
Cuts at: 3967 5038 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 659 399 246 235 132
85 78
BspEI T'CCGG_A
Cuts at: 2973 5484 2973
Size: 2511 3310
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2201 3176 844
Size: 1357 975 3489
Fragments arranged by size:
3489 1357 975
BsrBI CCG'CTC
Cuts at: 1394 1823 3708 1394
Size: 429 1885 3507
Fragments arranged by size:
3507 1885 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4316 4490 65
Size: 1183 3068 174 1396
Fragments arranged by size:
3068 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2343 4465 4652 1700
Size: 110 533 2122 187 2869
Fragments arranged by size:
2869 2122 533 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5821
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 2077 2808 2853 3151 3518 3903 3920 5372
Size: 105 731 45 298 367 385 17 1452
Cuts at: 5372 5458 5470 5816 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 731 385 367 346 298 105
86 45 17 12
BstZ17I GTA'TAC
Cuts at: 2508 2508
Size: 5821
Bsu36I CC'TnA_GG
Cuts at: 2027 2027
Size: 5821
BtgI C'CryG_G
Cuts at: 513 1065 2550 2716 513
Size: 552 1485 166 3618
Fragments arranged by size:
3618 1485 552 166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 2361 2644 3337 4131 4151 2361
Size: 283 693 794 20 4031
Fragments arranged by size:
4031 794 693 283 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DraIII CAC_nnn'GTG
Cuts at: 2485 2485
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4828 809
Size: 507 3512 1802
Fragments arranged by size:
3512 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3268 4157 5786
Size: 54 1425 50 275 18 1438 889 1629
Cuts at: 5786 8
Size: 43
Fragments arranged by size:
1629 1438 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 2249 3751 1353
Size: 755 141 1502 3423
Fragments arranged by size:
3423 1502 755 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4408 4782 4928 387
Size: 1064 138 2819 374 146 1280
Fragments arranged by size:
2819 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2387 2642 2651 2919 3884 4481
Size: 393 713 42 255 9 268 965 597
Cuts at: 4481 5268 5536 1239
Size: 787 268 1524
Fragments arranged by size:
1524 965 787 713 597 393 268 268
255 42 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2456 2456
Size: 5821
EcoO109I rG'GnC_Cy
Cuts at: 2142 2500 2142
Size: 358 5463
EcoRI G'AATT_C
Cuts at: 2791 3130 3508 2791
Size: 339 378 5104
Fragments arranged by size:
5104 378 339
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2319 2545 2319
Size: 226 5595
FspI TGC'GCA
Cuts at: 2931 4327 2931
Size: 1396 4425
HaeII r_GCGC'y
Cuts at: 1678 4968 1678
Size: 3290 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2594 2626 2769 2801 2879
Size: 32 135 32 1022 32 143 32 78
Cuts at: 2879 2911 4462 4494 4536 4568 1373
Size: 32 1551 32 42 32 2626
Fragments arranged by size:
2626 1551 1022 143 135 78 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2542 3165 3411 669
Size: 988 281 604 623 246 3079
Fragments arranged by size:
3079 988 623 604 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5821
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2437 4935 5119 1305
Size: 1132 2498 184 2007
Fragments arranged by size:
2498 2007 1132 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3999
Fragments arranged by size:
3999 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2474 2961 3110 3779 4138 4297 518
Size: 648 1308 487 149 669 359 159 2042
Fragments arranged by size:
2042 1308 669 648 487 359 159 149
MspA1I CmG'CkG
Cuts at: 1606 3237 3914 5062 5307 1606
Size: 1631 677 1148 245 2120
Fragments arranged by size:
2120 1631 1148 677 245
NaeI GCC'GGC
Cuts at: 2345 2345
Size: 5821
NcoI C'CATG_G
Cuts at: 513 1065 2550 513
Size: 552 1485 3784
Fragments arranged by size:
3784 1485 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NgoMIV G'CCGG_C
Cuts at: 2343 2343
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 2578 3179 4724 1132
Size: 97 1349 601 1545 2229
Fragments arranged by size:
2229 1545 1349 601 97
PasI CC'CwG_GG
Cuts at: 1348 2139 2271 2661 1348
Size: 791 132 390 4508
Fragments arranged by size:
4508 791 390 132
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2145 2839 1108
Size: 1037 694 4090
Fragments arranged by size:
4090 1037 694
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PmlI CAC'GTG
Cuts at: 2935 2935
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2952 1613
Size: 1339 4482
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PstI C_TGCA'G
Cuts at: 830 2388 2499 3173 830
Size: 1558 111 674 3478
Fragments arranged by size:
3478 1558 674 111
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SfcI C'TryA_G
Cuts at: 826 1045 2384 2495 3169 4304 4985 5176 826
Size: 219 1339 111 674 1135 681 191 1471
Fragments arranged by size:
1471 1339 1135 681 674 219 191 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3925 4826 5088 820
Size: 197 926 1982 901 262 1553
Fragments arranged by size:
1982 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 2578 3179 1132
Size: 1446 601 3774
Fragments arranged by size:
3774 1446 601
SspI AAT'ATT
Cuts at: 5 52 3019 3745 5
Size: 47 2967 726 2081
Fragments arranged by size:
2967 2081 726 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5821
StyI C'CwwG_G
Cuts at: 513 1065 2550 3260 513
Size: 552 1485 710 3074
Fragments arranged by size:
3074 1485 710 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3848 3865 4018 4203 4648 325
Size: 1255 2268 17 153 185 445 1498
Fragments arranged by size:
2268 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2823 4067 5779
Size: 274 80 33 51 1443 846 1244 1712
Cuts at: 5779 96
Size: 138
Fragments arranged by size:
1712 1443 1244 846 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5781 5813 5781
Size: 32 5789
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3022 3022
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI