Restriction map for
pFN21AB8727
(Circular) (Six-base) MAPSORT of: pFN21AB8727.seq Check: 7488 from: 1 to: 6181
pFN21AB8727 6181 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3991 278
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3085 3524 1937
Size: 1148 439 4594
Fragments arranged by size:
4594 1148 439
Acc65I G'GTAC_C
Cuts at: 3502 3502
Size: 6181
AclI AA'CG_TT
Cuts at: 1563 4308 4681 1563
Size: 2745 373 3063
Fragments arranged by size:
3063 2745 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4244 5628 5896 1632
Size: 2612 1384 268 1917
Fragments arranged by size:
2612 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5984
AflIII A'CryG_T
Cuts at: 1203 2382 3105 5080 6057 1203
Size: 1179 723 1975 977 1327
Fragments arranged by size:
1975 1327 1179 977 723
AgeI A'CCGG_T
Cuts at: 5012 5012
Size: 6181
AhdI GACnn_n'nnGTC
Cuts at: 2479 4910 2479
Size: 2431 3750
AleI CACnn'nnGTG
Cuts at: 1166 2339 3284 1166
Size: 1173 945 4063
Fragments arranged by size:
4063 1173 945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6181
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3023 3055 3023
Size: 32 6149
AlwNI CAG_nnn'CTG
Cuts at: 3070 3274 3924 5496 3070
Size: 204 650 1572 3755
Fragments arranged by size:
3755 1572 650 204
ApaI G_GGCC'C
Cuts at: 2098 2984 3407 2098
Size: 886 423 4872
Fragments arranged by size:
4872 886 423
ApaLI G'TGCA_C
Cuts at: 4238 5394 4238
Size: 1156 5025
ApoI r'AATT_y
Cuts at: 1493 3329 3338 3490 3716 3868 5977 1493
Size: 1836 9 152 226 152 2109 1697
Fragments arranged by size:
2109 1836 1697 226 152 152 9
AseI AT'TA_AT
Cuts at: 160 1032 4735 160
Size: 872 3703 1606
Fragments arranged by size:
3703 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
AvaI C'yCGr_G
Cuts at: 1870 1943 3409 3506 1870
Size: 73 1466 97 4545
Fragments arranged by size:
4545 1466 97 73
BamHI G'GATC_C
Cuts at: 3511 3878 3511
Size: 367 5814
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2250 2728 3502 4957 618
Size: 325 217 627 463 478 774 1455 1842
Fragments arranged by size:
1842 1455 774 627 478 463 325 217
BanII G_rGCy'C
Cuts at: 1391 2098 2748 2775 2817 2984 3041 3199 3407
Size: 707 650 27 42 167 57 158 208
Cuts at: 3407 3500 1391
Size: 93 4072
Fragments arranged by size:
4072 707 650 208 167 158 93 57
42 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3449 928
Size: 971 1550 3660
Fragments arranged by size:
3660 1550 971
BbvCI CC'TCA_GC
Cuts at: 3394 3399 3394
Size: 5 6176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4372 4406 1903
Size: 34 2435 34 3678
Fragments arranged by size:
3678 2435 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4073 5283 4073
Size: 1210 4971
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6181
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2957 4792 136
Size: 107 122 71 631 605 1285 1835 1525
Fragments arranged by size:
1835 1525 1285 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2109 6176 2109
Size: 4067 2114
BlpI GC'TnA_GC
Cuts at: 3598 3598
Size: 6181
Bme1580I G_kGCm'C
Cuts at: 1790 2098 2253 2552 2733 2984 3407 4242 5398
Size: 308 155 299 181 251 423 835 1156
Cuts at: 5398 1790
Size: 2573
Fragments arranged by size:
2573 1156 835 423 308 299 251 181
155
BmgBI CAC'GTC
Cuts at: 1318 3286 1318
Size: 1968 4213
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2101 3227 3912
Size: 416 53 83 399 24 684 1126 685
Cuts at: 3912 4870 442
Size: 958 1753
Fragments arranged by size:
1753 1126 958 685 684 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2022 2471 2542 2696 2777 2831 3025 3092
Size: 783 449 71 154 81 54 194 67
Cuts at: 3092 3213 3370 4841 1239
Size: 121 157 1471 2579
Fragments arranged by size:
2579 1471 783 449 194 157 154 121
81 71 67 54
Bpu10I CC'TnA_GC
Cuts at: 3394 3399 3394
Size: 5 6176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4306 5171 5469 4306
Size: 865 298 5018
Fragments arranged by size:
5018 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4844 882
Size: 617 3345 2219
Fragments arranged by size:
3345 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2263 2784 3972 493
Size: 711 1059 521 1188 2702
Fragments arranged by size:
2702 1188 1059 711 521
BsaBI GATnn'nnATC
Cuts at: 6175 6175
Size: 6181
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3204 3988 4370 5005
Size: 53 83 186 936 1671 784 382 635
Cuts at: 5005 275
Size: 1451
Fragments arranged by size:
1671 1451 936 784 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2618 4614 5012 5286 5433 5844 2618
Size: 1996 398 274 147 411 2955
Fragments arranged by size:
2955 1996 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3023 3053 1178
Size: 30 165 30 1620 30 4306
Fragments arranged by size:
4306 1620 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2864 1186
Size: 1678 4503
BseYI C'CCAG_C
Cuts at: 2989 3015 3427 5384 5880 2989
Size: 26 412 1957 496 3290
Fragments arranged by size:
3290 1957 496 412 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5608
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3631 4392 5420 1540
Size: 275 186 1630 761 1028 2301
Fragments arranged by size:
2301 1630 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2358 2775 2817 3221 4242 4327 5398 2358
Size: 417 42 404 1021 85 1071 3141
Fragments arranged by size:
3141 1071 1021 417 404 85 42
BsmI GAATG_Cn'
Cuts at: 3692 3785 3692
Size: 93 6088
BsmBI CGTCTCn'nnnn_
Cuts at: 2123 2123
Size: 6181
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 2253 2358 2552 2733 2748 2775
Size: 399 308 155 105 194 181 15 27
Cuts at: 2775 2817 2984 3041 3199 3221 3407 3500 4242
Size: 42 167 57 158 22 186 93 742
Cuts at: 4242 4327 5398 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 308 194 186 181
167 158 155 105 93 85 57 42
27 22 15
BspEI T'CCGG_A
Cuts at: 5844 5844
Size: 6181
BspHI T'CATG_A
Cuts at: 4070 5800 4070
Size: 1730 4451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2442 3536 844
Size: 1598 1094 3489
Fragments arranged by size:
3489 1598 1094
BsrBI CCG'CTC
Cuts at: 1394 1823 4068 1394
Size: 429 2245 3507
Fragments arranged by size:
3507 2245 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4676 4850 65
Size: 1183 3428 174 1396
Fragments arranged by size:
3428 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2956 2999 4825 5012 1700
Size: 110 1146 43 1826 187 2869
Fragments arranged by size:
2869 1826 1146 187 110 43
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6181
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6181
BssSI C'ACGA_G
Cuts at: 2198 4241 5253 2198
Size: 2043 1012 3126
Fragments arranged by size:
3126 2043 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6181
BstBI TT'CG_AA
Cuts at: 4985 4985
Size: 6181
BstEII G'GTnAC_C
Cuts at: 1184 2416 3316 1184
Size: 1232 900 4049
Fragments arranged by size:
4049 1232 900
BstXI CCAn_nnnn'nTGG
Cuts at: 3286 3286
Size: 6181
BstYI r'GATC_y
Cuts at: 1972 2109 3511 3878 4263 4280 5732 5818 5830
Size: 137 1402 367 385 17 1452 86 12
Cuts at: 5830 6176 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1402 385 367 346 137 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3309 3309
Size: 6181
BtgI C'CryG_G
Cuts at: 513 1065 2004 3363 513
Size: 552 939 1359 3331
Fragments arranged by size:
3331 1359 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5442
Fragments arranged by size:
5442 683 56
BtsI GCAGTG_nn'
Cuts at: 2674 3052 3275 3697 4491 4511 2674
Size: 378 223 422 794 20 4344
Fragments arranged by size:
4344 794 422 378 223 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6146
DraI TTT'AAA
Cuts at: 3485 3485
Size: 6181
DraIII CAC_nnn'GTG
Cuts at: 2203 2203
Size: 6181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5188 809
Size: 507 3872 1802
Fragments arranged by size:
3872 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2271 2578 3628
Size: 54 1425 50 275 18 441 307 1050
Cuts at: 3628 4517 6146 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1050 889 441 307 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3628 1537
Size: 275 1816 4090
Fragments arranged by size:
4090 1816 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 3297 4111 1353
Size: 812 1132 814 3423
Fragments arranged by size:
3423 1132 814 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4768 5142 5288 387
Size: 1064 138 3179 374 146 1280
Fragments arranged by size:
3179 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2773 2815 2773
Size: 42 6139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2022 2471 2542 2696 2777 2831 3025
Size: 393 390 449 71 154 81 54 194
Cuts at: 3025 3092 3213 3370 4244 4841 5628 5896 1239
Size: 67 121 157 874 597 787 268 1524
Fragments arranged by size:
1524 874 787 597 449 393 390 268
194 157 154 121 81 71 67 54
EcoNI CCTnn'n_nnAGG
Cuts at: 3254 3254
Size: 6181
EcoO109I rG'GnC_Cy
Cuts at: 2095 2141 2431 2806 2938 2981 3403 3404 3413
Size: 46 290 375 132 43 422 1 9
Cuts at: 3413 2095
Size: 4863
Fragments arranged by size:
4863 422 375 290 132 46 43 9
1
EcoRI G'AATT_C
Cuts at: 3490 3868 3490
Size: 378 5803
FspI TGC'GCA
Cuts at: 2666 4687 2666
Size: 2021 4160
HaeII r_GCGC'y
Cuts at: 1678 3434 5328 1678
Size: 1756 1894 2531
Fragments arranged by size:
2531 1894 1756
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2037 2069 2197 2229 2465
Size: 32 135 32 465 32 128 32 236
Cuts at: 2465 2497 2513 2545 4822 4854 4896 4928 1373
Size: 32 16 32 2277 32 42 32 2626
Fragments arranged by size:
2626 2277 465 236 135 128 42 32
32 32 32 32 32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3086 3525 3771 669
Size: 988 281 1148 439 246 3079
Fragments arranged by size:
3079 1148 988 439 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6181
HpaI GTT'AAC
Cuts at: 3771 3771
Size: 6181
KpnI G_GTAC'C
Cuts at: 3506 3506
Size: 6181
MfeI C'AATT_G
Cuts at: 3780 3780
Size: 6181
MluI A'CGCG_T
Cuts at: 2382 6057 2382
Size: 3675 2506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2688 5295 5479 1305
Size: 1383 2607 184 2007
Fragments arranged by size:
2607 2007 1383 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2273 2580 10
Size: 54 1425 343 441 307 3611
Fragments arranged by size:
3611 1425 441 343 307 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2339 3284 4139 4498 4657 518
Size: 648 1173 945 855 359 159 2042
Fragments arranged by size:
2042 1173 945 855 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2803 2842 2963 3019 3365 3398 3597 4274
Size: 1197 39 121 56 346 33 199 677
Cuts at: 4274 5422 5667 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1197 1148 677 346 245 199 121
56 39 33
NaeI GCC'GGC
Cuts at: 2958 2958
Size: 6181
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4690
Fragments arranged by size:
4690 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6181
NgoMIV G'CCGG_C
Cuts at: 2956 2956
Size: 6181
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6181
NotI GC'GGCC_GC
Cuts at: 3628 3628
Size: 6181
NspI r_CATG'y
Cuts at: 1132 1229 2479 2588 3109 3469 3539 5084 1132
Size: 97 1250 109 521 360 70 1545 2229
Fragments arranged by size:
2229 1545 1250 521 360 109 97 70
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6181
PciI A'CATG_T
Cuts at: 3105 5080 3105
Size: 1975 4206
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2266 1108
Size: 1158 5023
PfoI T'CCnGG_A
Cuts at: 2808 3342 2808
Size: 534 5647
PmeI GTTT'AAAC
Cuts at: 3485 3485
Size: 6181
PmlI CAC'GTG
Cuts at: 2263 2784 2263
Size: 521 5660
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3023 3055 4250 4282 5763 5795 3023
Size: 32 1195 32 1481 32 3409
Fragments arranged by size:
3409 1481 1195 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2141 2431 2806 3413 2141
Size: 290 375 607 4909
Fragments arranged by size:
4909 607 375 290
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6181
PsiI TTA'TAA
Cuts at: 3751 3751
Size: 6181
PspOMI G'GGCC_C
Cuts at: 2094 2980 3403 2094
Size: 886 423 4872
Fragments arranged by size:
4872 886 423
PstI C_TGCA'G
Cuts at: 830 2082 2769 3066 3533 830
Size: 1252 687 297 467 3478
Fragments arranged by size:
3478 1252 687 467 297
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6181
PvuII CAG'CTG
Cuts at: 2842 2963 3019 3398 2842
Size: 121 56 379 5625
Fragments arranged by size:
5625 379 121 56
RsrII CG'GwC_CG
Cuts at: 3367 3367
Size: 6181
SacI G_AGCT'C
Cuts at: 2775 2817 2775
Size: 42 6139
SacII CC_GC'GG
Cuts at: 3366 3366
Size: 6181
SalI G'TCGA_C
Cuts at: 1936 3084 3523 1936
Size: 1148 439 4594
Fragments arranged by size:
4594 1148 439
SanDI GG'GwC_CC
Cuts at: 3413 3413
Size: 6181
SapI GCTCTTCn'nnn_
Cuts at: 3297 3297
Size: 6181
SbfI CC_TGCA'GG
Cuts at: 3533 3533
Size: 6181
ScaI AGT'ACT
Cuts at: 4429 6141 4429
Size: 1712 4469
SfcI C'TryA_G
Cuts at: 826 1045 2078 2765 3062 3529 4664 5345 5536
Size: 219 1033 687 297 467 1135 681 191
Cuts at: 5536 826
Size: 1471
Fragments arranged by size:
1471 1135 1033 687 681 467 297 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6181
SmaI CCC'GGG
Cuts at: 1872 3411 3508 1872
Size: 1539 97 4545
Fragments arranged by size:
4545 1539 97
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4285 5186 5448 820
Size: 197 926 2342 901 262 1553
Fragments arranged by size:
2342 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3972 493
Size: 3479 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6181
SphI G_CATG'C
Cuts at: 1132 2588 3539 1132
Size: 1456 951 3774
Fragments arranged by size:
3774 1456 951
SspI AAT'ATT
Cuts at: 5 52 4105 5
Size: 47 4053 2081
Fragments arranged by size:
4053 2081 47
StuI AGG'CCT
Cuts at: 1517 2448 3077 3245 1517
Size: 931 629 168 4453
Fragments arranged by size:
4453 931 629 168
StyI C'CwwG_G
Cuts at: 513 1065 2004 2934 3620 513
Size: 552 939 930 686 3074
Fragments arranged by size:
3074 939 930 686 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2347 2500 2750 3254 3384 4208 4225
Size: 1255 767 153 250 504 130 824 17
Cuts at: 4225 4378 4563 5008 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 824 767 504 445 250 185
153 153 130 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4427 6139 96
Size: 274 80 33 51 1443 2450 1712 138
Fragments arranged by size:
2450 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6141 6173 6141
Size: 32 6149
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6181
XbaI T'CTAG_A
Cuts at: 3517 3517
Size: 6181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2467 2467
Size: 6181
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6181
XmaI C'CCGG_G
Cuts at: 1870 3409 3506 1870
Size: 1539 97 4545
Fragments arranged by size:
4545 1539 97
XmnI GAAnn'nnTTC
Cuts at: 2027 4310 2027
Size: 2283 3898
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3989 276
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PspXI PsrI SexAI SfiI SfoI
SrfI SwaI