Restriction map for
pFN21AB8726
(Circular) (Six-base) MAPSORT of: pFN21AB8726.seq Check: 2543 from: 1 to: 5779
pFN21AB8726 5779 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2775 3589 278
Size: 53 83 186 936 1239 814 2468
Fragments arranged by size:
2468 1239 936 814 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3122 1937
Size: 1185 4594
Acc65I G'GTAC_C
Cuts at: 3100 3100
Size: 5779
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3842 5226 5494 1632
Size: 2210 1384 268 1917
Fragments arranged by size:
2210 1917 1384 268
AfeI AGC'GCT
Cuts at: 2483 2483
Size: 5779
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 4678 5655 1203
Size: 3475 977 1327
Fragments arranged by size:
3475 1327 977
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 4508 4508
Size: 5779
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5779
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5779
AlwNI CAG_nnn'CTG
Cuts at: 3522 5094 3522
Size: 1572 4207
ApaLI G'TGCA_C
Cuts at: 3836 4992 3836
Size: 1156 4623
ApoI r'AATT_y
Cuts at: 1493 3088 3314 3466 5575 1493
Size: 1595 226 152 2109 1697
Fragments arranged by size:
2109 1697 1595 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 2178 2300 2555 2984 3104 1870
Size: 73 235 122 255 429 120 4545
Fragments arranged by size:
4545 429 255 235 122 120 73
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2980 3100 4555 618
Size: 325 217 627 1193 120 1455 1842
Fragments arranged by size:
1842 1455 1193 627 325 217 120
BanII G_rGCy'C
Cuts at: 1391 2326 3098 1391
Size: 935 772 4072
Fragments arranged by size:
4072 935 772
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2838 928
Size: 971 939 3869
Fragments arranged by size:
3869 971 939
BbvCI CC'TCA_GC
Cuts at: 2516 2804 3057 2516
Size: 288 253 5238
Fragments arranged by size:
5238 288 253
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3970 4004 1903
Size: 34 2033 34 3678
Fragments arranged by size:
3678 2033 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2693 3671 4881 2693
Size: 978 1210 3591
Fragments arranged by size:
3591 1210 978
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4390 136
Size: 107 122 71 631 605 2718 1525
Fragments arranged by size:
2718 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2235 5774 2235
Size: 3539 2240
BlpI GC'TnA_GC
Cuts at: 3196 3196
Size: 5779
Bme1580I G_kGCm'C
Cuts at: 1790 2985 3840 4996 1790
Size: 1195 855 1156 2573
Fragments arranged by size:
2573 1195 1156 855
BmgBI CAC'GTC
Cuts at: 1318 2338 2844 1318
Size: 1020 506 4253
Fragments arranged by size:
4253 1020 506
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3510 4468 442
Size: 416 53 83 399 24 2093 958 1753
Fragments arranged by size:
2093 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2623 3010 4439 1239
Size: 1384 387 1429 2579
Fragments arranged by size:
2579 1429 1384 387
Bpu10I CC'TnA_GC
Cuts at: 2516 2804 3057 2516
Size: 288 253 5238
Fragments arranged by size:
5238 288 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2090 2399 2938 3904 4769 5067 2090
Size: 309 539 966 865 298 2802
Fragments arranged by size:
2802 966 865 539 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2237 3014 4442 882
Size: 617 738 777 1428 2219
Fragments arranged by size:
2219 1428 777 738 617
BsaAI yAC'GTr
Cuts at: 493 1204 3570 493
Size: 711 2366 2702
Fragments arranged by size:
2702 2366 711
BsaBI GATnn'nnATC
Cuts at: 2189 2264 5773 2189
Size: 75 3509 2195
Fragments arranged by size:
3509 2195 75
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2283 2772 3586 3968
Size: 53 83 186 936 750 489 814 382
Cuts at: 3968 4603 275
Size: 635 1451
Fragments arranged by size:
1451 936 814 750 635 489 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3050 4212 4610 4884 5031 5442 3050
Size: 1162 398 274 147 411 3387
Fragments arranged by size:
3387 1162 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2198 2228 2864 2894 3045
Size: 30 165 30 795 30 636 30 151
Cuts at: 3045 3075 1178
Size: 30 3882
Fragments arranged by size:
3882 795 636 165 151 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 2356 2423 1186
Size: 1102 68 67 4542
Fragments arranged by size:
4542 1102 68 67
BseYI C'CCAG_C
Cuts at: 2961 4982 5478 2961
Size: 2021 496 3262
Fragments arranged by size:
3262 2021 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5206
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2137 3229 3990 5018 1540
Size: 275 186 136 1092 761 1028 2301
Fragments arranged by size:
2301 1092 1028 761 275 186 136
BsiHKAI G_wGCw'C
Cuts at: 2697 3840 3925 4996 2697
Size: 1143 85 1071 3480
Fragments arranged by size:
3480 1143 1071 85
BsmI GAATG_Cn'
Cuts at: 3290 3383 3290
Size: 93 5686
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2326 2697 2985 3098 3840 3925 4996
Size: 399 536 371 288 113 742 85 1071
Cuts at: 4996 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 536 399 371 288 113
85
BspEI T'CCGG_A
Cuts at: 3050 5442 3050
Size: 2392 3387
BspHI T'CATG_A
Cuts at: 3668 5398 3668
Size: 1730 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2738 3134 844
Size: 1894 396 3489
Fragments arranged by size:
3489 1894 396
BsrBI CCG'CTC
Cuts at: 1394 1823 3666 1394
Size: 429 1843 3507
Fragments arranged by size:
3507 1843 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2506 4274 4448 65
Size: 1183 1258 1768 174 1396
Fragments arranged by size:
1768 1396 1258 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2931 2967 4423 4610 1700
Size: 110 1121 36 1456 187 2869
Fragments arranged by size:
2869 1456 1121 187 110 36
BsrGI T'GTAC_A
Cuts at: 96 2622 96
Size: 2526 3253
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5779
BssSI C'ACGA_G
Cuts at: 2812 3839 4851 2812
Size: 1027 1012 3740
Fragments arranged by size:
3740 1027 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2546 2971 794
Size: 1752 425 3602
Fragments arranged by size:
3602 1752 425
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5779
BstXI CCAn_nnnn'nTGG
Cuts at: 2948 2948
Size: 5779
BstYI r'GATC_y
Cuts at: 1972 2235 2259 2799 3109 3476 3861 3878 5330
Size: 263 24 540 310 367 385 17 1452
Cuts at: 5330 5416 5428 5774 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 540 385 367 346 310 263
86 24 17 12
Bsu36I CC'TnA_GG
Cuts at: 2024 2024
Size: 5779
BtgI C'CryG_G
Cuts at: 513 1065 2004 2209 513
Size: 552 939 205 4083
Fragments arranged by size:
4083 939 552 205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5040
Fragments arranged by size:
5040 683 56
BtsI GCAGTG_nn'
Cuts at: 3295 4089 4109 3295
Size: 794 20 4965
Fragments arranged by size:
4965 794 20
ClaI AT'CG_AT
Cuts at: 1554 2793 1554
Size: 1239 4540
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5744
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DraIII CAC_nnn'GTG
Cuts at: 2701 2701
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4786 809
Size: 507 3470 1802
Fragments arranged by size:
3470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3015 3226 4115
Size: 54 1425 50 275 18 1185 211 889
Cuts at: 4115 5744 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1185 889 275 211 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3226 1537
Size: 275 1414 4090
Fragments arranged by size:
4090 1414 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3709 1353
Size: 2356 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2359 4366 4740 4886 387
Size: 1064 138 770 2007 374 146 1280
Fragments arranged by size:
2007 1280 1064 770 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2623 3010 3842 4439 5226 5494 1239
Size: 393 991 387 832 597 787 268 1524
Fragments arranged by size:
1524 991 832 787 597 393 387 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2330 2438 2860 2330
Size: 108 422 5249
Fragments arranged by size:
5249 422 108
EcoO109I rG'GnC_Cy
Cuts at: 2410 2743 2410
Size: 333 5446
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2932 2932
Size: 5779
FspI TGC'GCA
Cuts at: 4285 4285
Size: 5779
HaeII r_GCGC'y
Cuts at: 1678 2104 2485 4926 1678
Size: 426 381 2441 2531
Fragments arranged by size:
2531 2441 426 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4420 4452 4494 4526 1373
Size: 32 135 32 2848 32 42 32 2626
Fragments arranged by size:
2848 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 3123 3369 669
Size: 988 281 268 917 246 3079
Fragments arranged by size:
3079 988 917 281 268 246
HindIII A'AGCT_T
Cuts at: 748 2945 748
Size: 2197 3582
HpaI GTT'AAC
Cuts at: 3369 3369
Size: 5779
KpnI G_GTAC'C
Cuts at: 3104 3104
Size: 5779
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2380 2685 4893 5077 1305
Size: 1075 305 2208 184 2007
Fragments arranged by size:
2208 2007 1075 305 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3957
Fragments arranged by size:
3957 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2270 2793 3737 4096 4255 518
Size: 648 1104 523 944 359 159 2042
Fragments arranged by size:
2042 1104 944 648 523 359 159
MspA1I CmG'CkG
Cuts at: 1606 2782 3195 3872 5020 5265 1606
Size: 1176 413 677 1148 245 2120
Fragments arranged by size:
2120 1176 1148 677 413 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4288
Fragments arranged by size:
4288 939 552
NdeI CA'TA_TG
Cuts at: 387 2545 387
Size: 2158 3621
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 3226 3226
Size: 5779
NspI r_CATG'y
Cuts at: 1132 1229 2761 3137 4682 1132
Size: 97 1532 376 1545 2229
Fragments arranged by size:
2229 1545 1532 376 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5779
PciI A'CATG_T
Cuts at: 4678 4678
Size: 5779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2392 1108
Size: 1284 4495
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 5361 5393 3848
Size: 32 1481 32 4234
Fragments arranged by size:
4234 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2410 2743 2410
Size: 333 5446
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PspXI vC'TCGA_Gb
Cuts at: 2300 2300
Size: 5779
PstI C_TGCA'G
Cuts at: 830 2568 2766 2823 3131 830
Size: 1738 198 57 308 3478
Fragments arranged by size:
3478 1738 308 198 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SbfI CC_TGCA'GG
Cuts at: 3131 3131
Size: 5779
ScaI AGT'ACT
Cuts at: 2381 4027 5739 2381
Size: 1646 1712 2421
Fragments arranged by size:
2421 1712 1646
SfcI C'TryA_G
Cuts at: 826 1045 2564 2762 2819 3127 4262 4943 5134
Size: 219 1519 198 57 308 1135 681 191
Cuts at: 5134 826
Size: 1471
Fragments arranged by size:
1519 1471 1135 681 308 219 198 191
57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 3106 1872
Size: 1234 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2105 2300 2414 2917 3883 4784
Size: 197 926 162 195 114 503 966 901
Cuts at: 4784 5046 820
Size: 262 1553
Fragments arranged by size:
1553 966 926 901 503 262 197 195
162 114
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5779
SphI G_CATG'C
Cuts at: 1132 2761 3137 1132
Size: 1629 376 3774
Fragments arranged by size:
3774 1629 376
SspI AAT'ATT
Cuts at: 5 52 3703 5
Size: 47 3651 2081
Fragments arranged by size:
3651 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5779
StyI C'CwwG_G
Cuts at: 513 1065 2004 2586 3218 513
Size: 552 939 582 632 3074
Fragments arranged by size:
3074 939 632 582 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3806 3823 3976 4161 4606 325
Size: 1255 2226 17 153 185 445 1498
Fragments arranged by size:
2226 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 2622 4025
Size: 274 80 33 51 1443 402 243 1403
Cuts at: 4025 5737 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1403 402 274 243 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2402 2434 3045 3077 5739 5771 2402
Size: 32 611 32 2662 32 2410
Fragments arranged by size:
2662 2410 611 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2399 1316
Size: 1083 4696
XbaI T'CTAG_A
Cuts at: 3115 3115
Size: 5779
XhoI C'TCGA_G
Cuts at: 1943 2300 1943
Size: 357 5422
XmaI C'CCGG_G
Cuts at: 1870 3104 1870
Size: 1234 4545
XmnI GAAnn'nnTTC
Cuts at: 3908 3908
Size: 5779
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2773 3587 276
Size: 53 83 186 936 1239 814 2468
Fragments arranged by size:
2468 1239 936 814 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI