Restriction map for
pFN21AB8725
(Circular) (Six-base) MAPSORT of: pFN21AB8725.seq Check: 1468 from: 1 to: 6697
pFN21AB8725 6697 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4507 278
Size: 53 83 186 936 2971 2468
Fragments arranged by size:
2971 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2433 4040 1937
Size: 496 1607 4594
Fragments arranged by size:
4594 1607 496
Acc65I G'GTAC_C
Cuts at: 3071 4018 3071
Size: 947 5750
AclI AA'CG_TT
Cuts at: 1563 4824 5197 1563
Size: 3261 373 3063
Fragments arranged by size:
3261 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2268 3033 3793 4760 6144 6412 1632
Size: 636 765 760 967 1384 268 1917
Fragments arranged by size:
1917 1384 967 765 760 636 268
AfeI AGC'GCT
Cuts at: 2903 2903
Size: 6697
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6500
AflIII A'CryG_T
Cuts at: 1203 5596 6573 1203
Size: 4393 977 1327
Fragments arranged by size:
4393 1327 977
AgeI A'CCGG_T
Cuts at: 5528 5528
Size: 6697
AhdI GACnn_n'nnGTC
Cuts at: 5426 5426
Size: 6697
AleI CACnn'nnGTG
Cuts at: 1166 3626 1166
Size: 2460 4237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3867 1184
Size: 2683 4014
AlwNI CAG_nnn'CTG
Cuts at: 3552 4440 6012 3552
Size: 888 1572 4237
Fragments arranged by size:
4237 1572 888
ApaLI G'TGCA_C
Cuts at: 2878 3575 3852 4754 5910 2878
Size: 697 277 902 1156 3665
Fragments arranged by size:
3665 1156 902 697 277
ApoI r'AATT_y
Cuts at: 1493 4006 4232 4384 6493 1493
Size: 2513 226 152 2109 1697
Fragments arranged by size:
2513 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5251 160
Size: 872 4219 1606
Fragments arranged by size:
4219 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6697
AvaI C'yCGr_G
Cuts at: 1870 1943 2494 4022 1870
Size: 73 551 1528 4545
Fragments arranged by size:
4545 1528 551 73
AvrII C'CTAG_G
Cuts at: 3475 3475
Size: 6697
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3063 3096 3063
Size: 33 6664
BamHI G'GATC_C
Cuts at: 2424 4027 4394 2424
Size: 1603 367 4727
Fragments arranged by size:
4727 1603 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3071 3325 4018 5473 618
Size: 325 217 627 1284 254 693 1455 1842
Fragments arranged by size:
1842 1455 1284 693 627 325 254 217
BanII G_rGCy'C
Cuts at: 1391 2027 2070 3123 3182 3270 3546 4016 1391
Size: 636 43 1053 59 88 276 470 4072
Fragments arranged by size:
4072 1053 636 470 276 88 59 43
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3406 928
Size: 971 1507 4219
Fragments arranged by size:
4219 1507 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3298 3332 4888 4922 1903
Size: 34 1361 34 1556 34 3678
Fragments arranged by size:
3678 1556 1361 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4589 5799 4589
Size: 1210 5487
BclI T'GATC_A
Cuts at: 1548 2631 3003 1548
Size: 1083 372 5242
Fragments arranged by size:
5242 1083 372
BfrBI ATG'CAT
Cuts at: 3986 3986
Size: 6697
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5308 136
Size: 107 122 71 631 605 3636 1525
Fragments arranged by size:
3636 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2475 6692 2475
Size: 4217 2480
BlpI GC'TnA_GC
Cuts at: 4114 4114
Size: 6697
Bme1580I G_kGCm'C
Cuts at: 1790 2882 3330 3579 3856 4758 5914 1790
Size: 1092 448 249 277 902 1156 2573
Fragments arranged by size:
2573 1156 1092 902 448 277 249
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6697
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2283 2670 4428
Size: 416 53 83 399 24 866 387 1758
Cuts at: 4428 5386 442
Size: 958 1753
Fragments arranged by size:
1758 1753 958 866 416 399 387 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6697
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2240 2828 2240
Size: 588 6109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2041 5357 1239
Size: 802 3316 2579
Fragments arranged by size:
3316 2579 802
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4822 5687 5985 4822
Size: 865 298 5534
Fragments arranged by size:
5534 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2051 2814 3755 5360 882
Size: 617 552 763 941 1605 2219
Fragments arranged by size:
2219 1605 941 763 617 552
BsaAI yAC'GTr
Cuts at: 493 1204 2932 3500 4488 493
Size: 711 1728 568 988 2702
Fragments arranged by size:
2702 1728 988 711 568
BsaBI GATnn'nnATC
Cuts at: 2855 3308 3666 6691 2855
Size: 453 358 3025 2861
Fragments arranged by size:
3025 2861 453 358
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2694 4504 4886 5521
Size: 53 83 186 936 1161 1810 382 635
Cuts at: 5521 275
Size: 1451
Fragments arranged by size:
1810 1451 1161 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3658 5130 5528 5802 5949 6360 3658
Size: 1472 398 274 147 411 3995
Fragments arranged by size:
3995 1472 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6472
Fragments arranged by size:
6472 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3632 3722 3749 3890 1186
Size: 2446 90 27 141 3993
Fragments arranged by size:
3993 2446 141 90 27
BseYI C'CCAG_C
Cuts at: 2338 2372 3545 5900 6396 2338
Size: 34 1173 2355 496 2639
Fragments arranged by size:
2639 2355 1173 496 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2918 3356 1163
Size: 573 1182 438 4504
Fragments arranged by size:
4504 1182 573 438
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4147 4908 5936 1540
Size: 275 186 2146 761 1028 2301
Fragments arranged by size:
2301 2146 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 2882 3123 3182 3270 3579 3856 4758 4843
Size: 812 241 59 88 309 277 902 85
Cuts at: 4843 5914 2070
Size: 1071 2853
Fragments arranged by size:
2853 1071 902 812 309 277 241 88
85 59
BsmI GAATG_Cn'
Cuts at: 2320 3012 4208 4301 2320
Size: 692 1196 93 4716
Fragments arranged by size:
4716 1196 692 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2070 2882 3123 3182 3270 3330
Size: 399 237 43 812 241 59 88 60
Cuts at: 3330 3546 3579 3856 4016 4758 4843 5914 1391
Size: 216 33 277 160 742 85 1071 2174
Fragments arranged by size:
2174 1071 812 742 399 277 241 237
216 160 88 85 60 59 43 33
BspEI T'CCGG_A
Cuts at: 3658 6360 3658
Size: 2702 3995
BspHI T'CATG_A
Cuts at: 3174 3270 4586 6316 3174
Size: 96 1316 1730 3555
Fragments arranged by size:
3555 1730 1316 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4052 844
Size: 3208 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4584 1394
Size: 429 2761 3507
Fragments arranged by size:
3507 2761 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2083 3402 5192 5366 65
Size: 1183 835 1319 1790 174 1396
Fragments arranged by size:
1790 1396 1319 1183 835 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5341 5528 1700
Size: 110 3531 187 2869
Fragments arranged by size:
3531 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3018 96
Size: 2922 3775
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6697
BssSI C'ACGA_G
Cuts at: 2063 4757 5769 2063
Size: 2694 1012 2991
Fragments arranged by size:
2991 2694 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2567 3992 794
Size: 1773 1425 3499
Fragments arranged by size:
3499 1773 1425
BstBI TT'CG_AA
Cuts at: 5501 5501
Size: 6697
BstEII G'GTnAC_C
Cuts at: 1184 2041 3961 1184
Size: 857 1920 3920
Fragments arranged by size:
3920 1920 857
BstXI CCAn_nnnn'nTGG
Cuts at: 3391 3391
Size: 6697
BstYI r'GATC_y
Cuts at: 1972 2424 2475 2806 4027 4394 4779 4796 6248
Size: 452 51 331 1221 367 385 17 1452
Cuts at: 6248 6334 6346 6692 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1221 452 385 367 346 331
86 51 17 12
Bsu36I CC'TnA_GG
Cuts at: 2399 2521 3756 2399
Size: 122 1235 5340
Fragments arranged by size:
5340 1235 122
BtgI C'CryG_G
Cuts at: 513 1065 2004 2037 2828 3222 3966 513
Size: 552 939 33 791 394 744 3244
Fragments arranged by size:
3244 939 791 744 552 394 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2275 3079 3301 487
Size: 683 56 1049 804 222 3883
Fragments arranged by size:
3883 1049 804 683 222 56
BtsI GCAGTG_nn'
Cuts at: 2565 4213 5007 5027 2565
Size: 1648 794 20 4235
Fragments arranged by size:
4235 1648 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6697
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3093 3128 529
Size: 35 2529 35 4098
Fragments arranged by size:
4098 2529 35 35
DraI TTT'AAA
Cuts at: 4001 4001
Size: 6697
DraIII CAC_nnn'GTG
Cuts at: 2257 2680 3350 2257
Size: 423 670 5604
Fragments arranged by size:
5604 670 423
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5704 809
Size: 507 4388 1802
Fragments arranged by size:
4388 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2207 2248
Size: 54 1425 50 275 18 294 83 41
Cuts at: 2248 2265 4144 5033 6662 8
Size: 17 1879 889 1629 43
Fragments arranged by size:
1879 1629 1425 889 294 275 83 54
50 43 41 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 4144 1537
Size: 275 2332 4090
Fragments arranged by size:
4090 2332 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3221 3365 3431 3608 3726 3867 4627 1353
Size: 1868 144 66 177 118 141 760 3423
Fragments arranged by size:
3423 1868 760 177 144 141 118 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5284 5658 5804 387
Size: 1064 138 3695 374 146 1280
Fragments arranged by size:
3695 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2068 3121 3180 3268 2068
Size: 1053 59 88 5497
Fragments arranged by size:
5497 1053 88 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2268 3033 3793 4760 5357 6144
Size: 393 409 227 765 760 967 597 787
Cuts at: 6144 6412 1239
Size: 268 1524
Fragments arranged by size:
1524 967 787 765 760 597 409 393
268 227
EcoO109I rG'GnC_Cy
Cuts at: 2590 3752 2590
Size: 1162 5535
EcoRI G'AATT_C
Cuts at: 4006 4384 4006
Size: 378 6319
EcoRV GAT'ATC
Cuts at: 2089 2089
Size: 6697
FspI TGC'GCA
Cuts at: 3695 5203 3695
Size: 1508 5189
HaeII r_GCGC'y
Cuts at: 1678 2905 3207 5844 1678
Size: 1227 302 2637 2531
Fragments arranged by size:
2637 2531 1227 302
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 5338 5370 5412
Size: 32 135 32 905 32 2829 32 42
Cuts at: 5412 5444 1373
Size: 32 2626
Fragments arranged by size:
2829 2626 905 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4041 4287 669
Size: 988 281 2103 246 3079
Fragments arranged by size:
3079 2103 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6697
HpaI GTT'AAC
Cuts at: 4287 4287
Size: 6697
KpnI G_GTAC'C
Cuts at: 3075 4022 3075
Size: 947 5750
MfeI C'AATT_G
Cuts at: 4296 4296
Size: 6697
MluI A'CGCG_T
Cuts at: 6573 6573
Size: 6697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2110 2149 2750 3128 3665 5811 5995 1305
Size: 805 39 601 378 537 2146 184 2007
Fragments arranged by size:
2146 2007 805 601 537 378 184 39
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2250 10
Size: 54 1425 343 418 4457
Fragments arranged by size:
4457 1425 418 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2639 2987 2990 3626 3890 3959 4655
Size: 648 1473 348 3 636 264 69 696
Cuts at: 4655 5014 5173 518
Size: 359 159 2042
Fragments arranged by size:
2042 1473 696 648 636 359 348 264
159 69 3
MspA1I CmG'CkG
Cuts at: 1606 2039 2422 4113 4790 5938 6183 1606
Size: 433 383 1691 677 1148 245 2120
Fragments arranged by size:
2120 1691 1148 677 433 383 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2828 3222 513
Size: 552 939 824 394 3988
Fragments arranged by size:
3988 939 824 552 394
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6697
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6697
NotI GC'GGCC_GC
Cuts at: 4144 4144
Size: 6697
NsiI A_TGCA'T
Cuts at: 3988 3988
Size: 6697
NspI r_CATG'y
Cuts at: 1132 1229 4055 5600 1132
Size: 97 2826 1545 2229
Fragments arranged by size:
2826 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6697
PciI A'CATG_T
Cuts at: 5596 5596
Size: 6697
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 2834 1108
Size: 1149 577 4971
Fragments arranged by size:
4971 1149 577
PmeI GTTT'AAAC
Cuts at: 4001 4001
Size: 6697
PmlI CAC'GTG
Cuts at: 2932 2932
Size: 6697
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4766 4798 6279 6311 4766
Size: 32 1481 32 5152
Fragments arranged by size:
5152 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6697
PsiI TTA'TAA
Cuts at: 4267 4267
Size: 6697
PstI C_TGCA'G
Cuts at: 830 4049 830
Size: 3219 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6697
SacI G_AGCT'C
Cuts at: 2070 3123 3182 3270 2070
Size: 1053 59 88 5497
Fragments arranged by size:
5497 1053 88 59
SacII CC_GC'GG
Cuts at: 2040 2040
Size: 6697
SalI G'TCGA_C
Cuts at: 1936 4039 1936
Size: 2103 4594
SbfI CC_TGCA'GG
Cuts at: 4049 4049
Size: 6697
ScaI AGT'ACT
Cuts at: 4945 6657 4945
Size: 1712 4985
SfcI C'TryA_G
Cuts at: 826 1045 4045 5180 5861 6052 826
Size: 219 3000 1135 681 191 1471
Fragments arranged by size:
3000 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6697
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6697
SmaI CCC'GGG
Cuts at: 1872 4024 1872
Size: 2152 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4801 5702 5964 820
Size: 197 926 2858 901 262 1553
Fragments arranged by size:
2858 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4488 493
Size: 3995 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6697
SphI G_CATG'C
Cuts at: 1132 4055 1132
Size: 2923 3774
SspI AAT'ATT
Cuts at: 5 52 3065 4621 5
Size: 47 3013 1556 2081
Fragments arranged by size:
3013 2081 1556 47
StuI AGG'CCT
Cuts at: 1517 2526 3144 1517
Size: 1009 618 5070
Fragments arranged by size:
5070 1009 618
StyI C'CwwG_G
Cuts at: 513 1065 2004 2777 2828 3075 3222 3237 3475
Size: 552 939 773 51 247 147 15 238
Cuts at: 3475 4136 513
Size: 661 3074
Fragments arranged by size:
3074 939 773 661 552 247 238 147
51 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3313 3415 3871 4724 4741 4894 5079
Size: 1255 1733 102 456 853 17 153 185
Cuts at: 5079 5524 325
Size: 445 1498
Fragments arranged by size:
1733 1498 1255 853 456 445 185 153
102 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2709 3018 3585
Size: 274 80 33 51 1443 732 309 567
Cuts at: 3585 4943 6655 96
Size: 1358 1712 138
Fragments arranged by size:
1712 1443 1358 732 567 309 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6657 6689 6657
Size: 32 6665
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6697
XbaI T'CTAG_A
Cuts at: 4033 4033
Size: 6697
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2983 3459 2983
Size: 476 6221
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6697
XmaI C'CCGG_G
Cuts at: 1870 4022 1870
Size: 2152 4545
XmnI GAAnn'nnTTC
Cuts at: 4826 4826
Size: 6697
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4505 276
Size: 53 83 186 936 2971 2468
Fragments arranged by size:
2971 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BbeI BbvCI Bpu10I BsiWI
BsmBI BstZ17I EcoNI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI
PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI
SrfI SwaI