(Circular) (Six-base) MAPSORT of: pFN21AB8724.seq Check: 8322 from: 1 to: 6286 pFN21AB8724 6286 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4096 278 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2376 3501 3629 1937 Size: 439 1125 128 4594 Fragments arranged by size: 4594 1125 439 128 Acc65I G'GTAC_C Cuts at: 3607 3607 Size: 6286 AclI AA'CG_TT Cuts at: 1563 4413 4786 1563 Size: 2850 373 3063 Fragments arranged by size: 3063 2850 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2615 2701 3335 4349 5733 6001 1632 Size: 983 86 634 1014 1384 268 1917 Fragments arranged by size: 1917 1384 1014 983 634 268 86 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6089 AflIII A'CryG_T Cuts at: 1203 5185 6162 1203 Size: 3982 977 1327 Fragments arranged by size: 3982 1327 977 AgeI A'CCGG_T Cuts at: 5117 5117 Size: 6286 AhdI GACnn_n'nnGTC Cuts at: 2383 5015 2383 Size: 2632 3654 AleI CACnn'nnGTG Cuts at: 1166 3449 1166 Size: 2283 4003 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3167 1184 Size: 1983 4303 AlwNI CAG_nnn'CTG Cuts at: 2405 2855 3106 4029 5601 2405 Size: 450 251 923 1572 3090 Fragments arranged by size: 3090 1572 923 450 251 ApaLI G'TGCA_C Cuts at: 2618 4343 5499 2618 Size: 1725 1156 3405 Fragments arranged by size: 3405 1725 1156 ApoI r'AATT_y Cuts at: 1493 2725 2993 3275 3595 3821 3973 6082 1493 Size: 1232 268 282 320 226 152 2109 1697 Fragments arranged by size: 2109 1697 1232 320 282 268 226 152 AseI AT'TA_AT Cuts at: 160 1032 4840 160 Size: 872 3808 1606 Fragments arranged by size: 3808 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 AvaI C'yCGr_G Cuts at: 1870 1943 3213 3255 3611 1870 Size: 73 1270 42 356 4545 Fragments arranged by size: 4545 1270 356 73 42 BamHI G'GATC_C Cuts at: 2454 2978 3616 3983 2454 Size: 524 638 367 4757 Fragments arranged by size: 4757 638 524 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3434 3607 5062 618 Size: 325 217 627 1647 173 1455 1842 Fragments arranged by size: 1842 1647 1455 627 325 217 173 BanII G_rGCy'C Cuts at: 1391 3605 1391 Size: 2214 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2358 3194 928 Size: 971 459 836 4020 Fragments arranged by size: 4020 971 836 459 BbvCI CC'TCA_GC Cuts at: 2826 2826 Size: 6286 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4477 4511 1903 Size: 34 2540 34 3678 Fragments arranged by size: 3678 2540 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4178 5388 4178 Size: 1210 5076 BclI T'GATC_A Cuts at: 1548 1548 Size: 6286 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4897 136 Size: 107 122 71 631 605 3225 1525 Fragments arranged by size: 3225 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6281 6281 Size: 6286 BlpI GC'TnA_GC Cuts at: 3703 3703 Size: 6286 Bme1580I G_kGCm'C Cuts at: 1790 2622 4347 5503 1790 Size: 832 1725 1156 2573 Fragments arranged by size: 2573 1725 1156 832 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6286 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2203 2993 4017 Size: 416 53 83 399 24 786 790 1024 Cuts at: 4017 4975 442 Size: 958 1753 Fragments arranged by size: 1753 1024 958 790 786 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6286 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2262 2262 Size: 6286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2354 2414 2942 3107 3305 4946 1239 Size: 1115 60 528 165 198 1641 2579 Fragments arranged by size: 2579 1641 1115 528 198 165 60 Bpu10I CC'TnA_GC Cuts at: 2237 2826 2237 Size: 589 5697 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4411 5276 5574 4411 Size: 865 298 5123 Fragments arranged by size: 5123 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2597 4949 882 Size: 617 1098 2352 2219 Fragments arranged by size: 2352 2219 1098 617 BsaAI yAC'GTr Cuts at: 493 1204 3136 4077 493 Size: 711 1932 941 2702 Fragments arranged by size: 2702 1932 941 711 BsaBI GATnn'nnATC Cuts at: 3326 3386 6280 3326 Size: 60 2894 3332 Fragments arranged by size: 3332 2894 60 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4093 4475 5110 275 Size: 53 83 186 936 2560 382 635 1451 Fragments arranged by size: 2560 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4719 5117 5391 5538 5949 4719 Size: 398 274 147 411 5056 Fragments arranged by size: 5056 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6061 Fragments arranged by size: 6061 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2324 1186 Size: 1138 5148 BseYI C'CCAG_C Cuts at: 2069 2417 3575 5489 5985 2069 Size: 348 1158 1914 496 2370 Fragments arranged by size: 2370 1914 1158 496 348 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5713 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3505 3736 4497 5525 1540 Size: 275 186 1504 231 761 1028 2301 Fragments arranged by size: 2301 1504 1028 761 275 231 186 BsiHKAI G_wGCw'C Cuts at: 2622 4347 4432 5503 2622 Size: 1725 85 1071 3405 Fragments arranged by size: 3405 1725 1071 85 BsmI GAATG_Cn' Cuts at: 3797 3890 3797 Size: 93 6193 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2622 3605 4347 4432 5503 1391 Size: 399 832 983 742 85 1071 2174 Fragments arranged by size: 2174 1071 983 832 742 399 85 BspEI T'CCGG_A Cuts at: 5949 5949 Size: 6286 BspHI T'CATG_A Cuts at: 2886 4175 5905 2886 Size: 1289 1730 3267 Fragments arranged by size: 3267 1730 1289 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3570 3641 844 Size: 2726 71 3489 Fragments arranged by size: 3489 2726 71 BsrBI CCG'CTC Cuts at: 1394 1823 4173 1394 Size: 429 2350 3507 Fragments arranged by size: 3507 2350 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4781 4955 65 Size: 1183 3533 174 1396 Fragments arranged by size: 3533 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4930 5117 1700 Size: 110 3120 187 2869 Fragments arranged by size: 3120 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6286 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6286 BssSI C'ACGA_G Cuts at: 4346 5358 4346 Size: 1012 5274 BstAPI GCAn_nnn'nTGC Cuts at: 794 3543 794 Size: 2749 3537 BstBI TT'CG_AA Cuts at: 5090 5090 Size: 6286 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6286 BstYI r'GATC_y Cuts at: 1972 2203 2454 2978 3616 3983 4368 4385 5837 Size: 231 251 524 638 367 385 17 1452 Cuts at: 5837 5923 5935 6281 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 638 524 385 367 346 251 231 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2587 2587 Size: 6286 BtgI C'CryG_G Cuts at: 513 1065 3456 513 Size: 552 2391 3343 Fragments arranged by size: 3343 2391 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2585 2693 3518 487 Size: 683 56 1359 108 825 3255 Fragments arranged by size: 3255 1359 825 683 108 56 BtsI GCAGTG_nn' Cuts at: 2738 3802 4596 4616 2738 Size: 1064 794 20 4408 Fragments arranged by size: 4408 1064 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6286 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6251 DraI TTT'AAA Cuts at: 2638 3590 2638 Size: 952 5334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5293 809 Size: 507 3977 1802 Fragments arranged by size: 3977 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3567 3733 4622 Size: 54 1425 50 275 18 1737 166 889 Cuts at: 4622 6251 8 Size: 1629 43 Fragments arranged by size: 1737 1629 1425 889 275 166 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3733 1537 Size: 275 1921 4090 Fragments arranged by size: 4090 1921 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2253 2696 2720 3556 3557 4216 1353 Size: 900 443 24 836 1 659 3423 Fragments arranged by size: 3423 900 836 659 443 24 1 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2071 4873 5247 5393 387 Size: 1064 138 482 2802 374 146 1280 Fragments arranged by size: 2802 1280 1064 482 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2354 2414 2615 2701 2942 3107 3305 Size: 393 722 60 201 86 241 165 198 Cuts at: 3305 3335 4349 4946 5733 6001 1239 Size: 30 1014 597 787 268 1524 Fragments arranged by size: 1524 1014 787 722 597 393 268 241 201 198 165 86 60 30 EcoO109I rG'GnC_Cy Cuts at: 2902 2902 Size: 6286 EcoRI G'AATT_C Cuts at: 3595 3973 3595 Size: 378 5908 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3097 3097 Size: 6286 FspI TGC'GCA Cuts at: 4792 4792 Size: 6286 HaeII r_GCGC'y Cuts at: 1678 5433 1678 Size: 3755 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2369 2401 2446 2478 4927 Size: 32 135 32 797 32 45 32 2449 Cuts at: 4927 4959 5001 5033 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2449 797 135 45 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2377 2524 3502 3630 3876 669 Size: 988 281 439 147 978 128 246 3079 Fragments arranged by size: 3079 988 978 439 281 246 147 128 HindIII A'AGCT_T Cuts at: 748 748 Size: 6286 HpaI GTT'AAC Cuts at: 3876 3876 Size: 6286 KpnI G_GTAC'C Cuts at: 3611 3611 Size: 6286 MfeI C'AATT_G Cuts at: 3885 3885 Size: 6286 MluI A'CGCG_T Cuts at: 6162 6162 Size: 6286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5400 5584 1305 Size: 4095 184 2007 Fragments arranged by size: 4095 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3569 10 Size: 54 1425 343 1737 2727 Fragments arranged by size: 2727 1737 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2137 3149 3449 4244 4603 4762 Size: 648 843 128 1012 300 795 359 159 Cuts at: 4762 518 Size: 2042 Fragments arranged by size: 2042 1012 843 795 648 359 300 159 128 MspA1I CmG'CkG Cuts at: 1606 2020 2161 3127 3475 3543 3702 4379 5527 Size: 414 141 966 348 68 159 677 1148 Cuts at: 5527 5772 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 966 677 414 348 245 159 141 68 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5734 NdeI CA'TA_TG Cuts at: 387 387 Size: 6286 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6286 NotI GC'GGCC_GC Cuts at: 3733 3733 Size: 6286 NspI r_CATG'y Cuts at: 1132 1229 2245 3154 3644 5189 1132 Size: 97 1016 909 490 1545 2229 Fragments arranged by size: 2229 1545 1016 909 490 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6286 PciI A'CATG_T Cuts at: 5185 5185 Size: 6286 PflMI CCAn_nnn'nTGG Cuts at: 1108 2292 3106 1108 Size: 1184 814 4288 Fragments arranged by size: 4288 1184 814 PmeI GTTT'AAAC Cuts at: 3590 3590 Size: 6286 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4355 4387 5868 5900 4355 Size: 32 1481 32 4741 Fragments arranged by size: 4741 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6286 PsiI TTA'TAA Cuts at: 3856 3856 Size: 6286 PstI C_TGCA'G Cuts at: 830 2019 2203 3638 830 Size: 1189 184 1435 3478 Fragments arranged by size: 3478 1435 1189 184 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6286 PvuII CAG'CTG Cuts at: 3127 3543 3127 Size: 416 5870 SalI G'TCGA_C Cuts at: 1936 2375 3500 3628 1936 Size: 439 1125 128 4594 Fragments arranged by size: 4594 1125 439 128 SapI GCTCTTCn'nnn_ Cuts at: 3557 3557 Size: 6286 SbfI CC_TGCA'GG Cuts at: 3638 3638 Size: 6286 ScaI AGT'ACT Cuts at: 4534 6246 4534 Size: 1712 4574 SexAI A'CCwGG_T Cuts at: 2517 2517 Size: 6286 SfcI C'TryA_G Cuts at: 826 1045 2015 2199 3634 4769 5450 5641 826 Size: 219 970 184 1435 1135 681 191 1471 Fragments arranged by size: 1471 1435 1135 970 681 219 191 184 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6286 SmaI CCC'GGG Cuts at: 1872 3215 3613 1872 Size: 1343 398 4545 Fragments arranged by size: 4545 1343 398 SmlI C'TyrA_G Cuts at: 820 1017 1943 3255 4390 5291 5553 820 Size: 197 926 1312 1135 901 262 1553 Fragments arranged by size: 1553 1312 1135 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4077 493 Size: 3584 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6286 SphI G_CATG'C Cuts at: 1132 2245 3154 3644 1132 Size: 1113 909 490 3774 Fragments arranged by size: 3774 1113 909 490 SspI AAT'ATT Cuts at: 5 52 4210 5 Size: 47 4158 2081 Fragments arranged by size: 4158 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6286 StyI C'CwwG_G Cuts at: 513 1065 2898 3072 3406 3725 513 Size: 552 1833 174 334 319 3074 Fragments arranged by size: 3074 1833 552 334 319 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2974 3452 3497 4313 4330 4483 4668 Size: 1255 1394 478 45 816 17 153 185 Cuts at: 4668 5113 325 Size: 445 1498 Fragments arranged by size: 1498 1394 1255 816 478 445 185 153 45 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4532 6244 96 Size: 274 80 33 51 1443 2555 1712 138 Fragments arranged by size: 2555 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2970 3002 6246 6278 2970 Size: 32 3244 32 2978 Fragments arranged by size: 3244 2978 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2372 2519 1316 Size: 1056 147 5083 Fragments arranged by size: 5083 1056 147 XbaI T'CTAG_A Cuts at: 3622 3622 Size: 6286 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2438 2438 Size: 6286 XhoI C'TCGA_G Cuts at: 1943 3255 1943 Size: 1312 4974 XmaI C'CCGG_G Cuts at: 1870 3213 3611 1870 Size: 1343 398 4545 Fragments arranged by size: 4545 1343 398 XmnI GAAnn'nnTTC Cuts at: 2342 2993 3264 4415 2342 Size: 651 271 1151 4213 Fragments arranged by size: 4213 1151 651 271 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4094 276 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI