Restriction map for
pFN21AB8724
(Circular) (Six-base) MAPSORT of: pFN21AB8724.seq Check: 8322 from: 1 to: 6286
pFN21AB8724 6286 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4096 278
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2376 3501 3629 1937
Size: 439 1125 128 4594
Fragments arranged by size:
4594 1125 439 128
Acc65I G'GTAC_C
Cuts at: 3607 3607
Size: 6286
AclI AA'CG_TT
Cuts at: 1563 4413 4786 1563
Size: 2850 373 3063
Fragments arranged by size:
3063 2850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2615 2701 3335 4349 5733 6001 1632
Size: 983 86 634 1014 1384 268 1917
Fragments arranged by size:
1917 1384 1014 983 634 268 86
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6089
AflIII A'CryG_T
Cuts at: 1203 5185 6162 1203
Size: 3982 977 1327
Fragments arranged by size:
3982 1327 977
AgeI A'CCGG_T
Cuts at: 5117 5117
Size: 6286
AhdI GACnn_n'nnGTC
Cuts at: 2383 5015 2383
Size: 2632 3654
AleI CACnn'nnGTG
Cuts at: 1166 3449 1166
Size: 2283 4003
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3167 1184
Size: 1983 4303
AlwNI CAG_nnn'CTG
Cuts at: 2405 2855 3106 4029 5601 2405
Size: 450 251 923 1572 3090
Fragments arranged by size:
3090 1572 923 450 251
ApaLI G'TGCA_C
Cuts at: 2618 4343 5499 2618
Size: 1725 1156 3405
Fragments arranged by size:
3405 1725 1156
ApoI r'AATT_y
Cuts at: 1493 2725 2993 3275 3595 3821 3973 6082 1493
Size: 1232 268 282 320 226 152 2109 1697
Fragments arranged by size:
2109 1697 1232 320 282 268 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4840 160
Size: 872 3808 1606
Fragments arranged by size:
3808 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
AvaI C'yCGr_G
Cuts at: 1870 1943 3213 3255 3611 1870
Size: 73 1270 42 356 4545
Fragments arranged by size:
4545 1270 356 73 42
BamHI G'GATC_C
Cuts at: 2454 2978 3616 3983 2454
Size: 524 638 367 4757
Fragments arranged by size:
4757 638 524 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3434 3607 5062 618
Size: 325 217 627 1647 173 1455 1842
Fragments arranged by size:
1842 1647 1455 627 325 217 173
BanII G_rGCy'C
Cuts at: 1391 3605 1391
Size: 2214 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2358 3194 928
Size: 971 459 836 4020
Fragments arranged by size:
4020 971 836 459
BbvCI CC'TCA_GC
Cuts at: 2826 2826
Size: 6286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4477 4511 1903
Size: 34 2540 34 3678
Fragments arranged by size:
3678 2540 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4178 5388 4178
Size: 1210 5076
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6286
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4897 136
Size: 107 122 71 631 605 3225 1525
Fragments arranged by size:
3225 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6281 6281
Size: 6286
BlpI GC'TnA_GC
Cuts at: 3703 3703
Size: 6286
Bme1580I G_kGCm'C
Cuts at: 1790 2622 4347 5503 1790
Size: 832 1725 1156 2573
Fragments arranged by size:
2573 1725 1156 832
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6286
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2203 2993 4017
Size: 416 53 83 399 24 786 790 1024
Cuts at: 4017 4975 442
Size: 958 1753
Fragments arranged by size:
1753 1024 958 790 786 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6286
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2262 2262
Size: 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2354 2414 2942 3107 3305 4946 1239
Size: 1115 60 528 165 198 1641 2579
Fragments arranged by size:
2579 1641 1115 528 198 165 60
Bpu10I CC'TnA_GC
Cuts at: 2237 2826 2237
Size: 589 5697
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4411 5276 5574 4411
Size: 865 298 5123
Fragments arranged by size:
5123 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2597 4949 882
Size: 617 1098 2352 2219
Fragments arranged by size:
2352 2219 1098 617
BsaAI yAC'GTr
Cuts at: 493 1204 3136 4077 493
Size: 711 1932 941 2702
Fragments arranged by size:
2702 1932 941 711
BsaBI GATnn'nnATC
Cuts at: 3326 3386 6280 3326
Size: 60 2894 3332
Fragments arranged by size:
3332 2894 60
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4093 4475 5110 275
Size: 53 83 186 936 2560 382 635 1451
Fragments arranged by size:
2560 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4719 5117 5391 5538 5949 4719
Size: 398 274 147 411 5056
Fragments arranged by size:
5056 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6061
Fragments arranged by size:
6061 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2324 1186
Size: 1138 5148
BseYI C'CCAG_C
Cuts at: 2069 2417 3575 5489 5985 2069
Size: 348 1158 1914 496 2370
Fragments arranged by size:
2370 1914 1158 496 348
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5713
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3505 3736 4497 5525 1540
Size: 275 186 1504 231 761 1028 2301
Fragments arranged by size:
2301 1504 1028 761 275 231 186
BsiHKAI G_wGCw'C
Cuts at: 2622 4347 4432 5503 2622
Size: 1725 85 1071 3405
Fragments arranged by size:
3405 1725 1071 85
BsmI GAATG_Cn'
Cuts at: 3797 3890 3797
Size: 93 6193
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2622 3605 4347 4432 5503 1391
Size: 399 832 983 742 85 1071 2174
Fragments arranged by size:
2174 1071 983 832 742 399 85
BspEI T'CCGG_A
Cuts at: 5949 5949
Size: 6286
BspHI T'CATG_A
Cuts at: 2886 4175 5905 2886
Size: 1289 1730 3267
Fragments arranged by size:
3267 1730 1289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3570 3641 844
Size: 2726 71 3489
Fragments arranged by size:
3489 2726 71
BsrBI CCG'CTC
Cuts at: 1394 1823 4173 1394
Size: 429 2350 3507
Fragments arranged by size:
3507 2350 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4781 4955 65
Size: 1183 3533 174 1396
Fragments arranged by size:
3533 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4930 5117 1700
Size: 110 3120 187 2869
Fragments arranged by size:
3120 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6286
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6286
BssSI C'ACGA_G
Cuts at: 4346 5358 4346
Size: 1012 5274
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3543 794
Size: 2749 3537
BstBI TT'CG_AA
Cuts at: 5090 5090
Size: 6286
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6286
BstYI r'GATC_y
Cuts at: 1972 2203 2454 2978 3616 3983 4368 4385 5837
Size: 231 251 524 638 367 385 17 1452
Cuts at: 5837 5923 5935 6281 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 638 524 385 367 346 251
231 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2587 2587
Size: 6286
BtgI C'CryG_G
Cuts at: 513 1065 3456 513
Size: 552 2391 3343
Fragments arranged by size:
3343 2391 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2585 2693 3518 487
Size: 683 56 1359 108 825 3255
Fragments arranged by size:
3255 1359 825 683 108 56
BtsI GCAGTG_nn'
Cuts at: 2738 3802 4596 4616 2738
Size: 1064 794 20 4408
Fragments arranged by size:
4408 1064 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6251
DraI TTT'AAA
Cuts at: 2638 3590 2638
Size: 952 5334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5293 809
Size: 507 3977 1802
Fragments arranged by size:
3977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3567 3733 4622
Size: 54 1425 50 275 18 1737 166 889
Cuts at: 4622 6251 8
Size: 1629 43
Fragments arranged by size:
1737 1629 1425 889 275 166 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3733 1537
Size: 275 1921 4090
Fragments arranged by size:
4090 1921 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2253 2696 2720 3556 3557 4216 1353
Size: 900 443 24 836 1 659 3423
Fragments arranged by size:
3423 900 836 659 443 24 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2071 4873 5247 5393 387
Size: 1064 138 482 2802 374 146 1280
Fragments arranged by size:
2802 1280 1064 482 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2354 2414 2615 2701 2942 3107 3305
Size: 393 722 60 201 86 241 165 198
Cuts at: 3305 3335 4349 4946 5733 6001 1239
Size: 30 1014 597 787 268 1524
Fragments arranged by size:
1524 1014 787 722 597 393 268 241
201 198 165 86 60 30
EcoO109I rG'GnC_Cy
Cuts at: 2902 2902
Size: 6286
EcoRI G'AATT_C
Cuts at: 3595 3973 3595
Size: 378 5908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3097 3097
Size: 6286
FspI TGC'GCA
Cuts at: 4792 4792
Size: 6286
HaeII r_GCGC'y
Cuts at: 1678 5433 1678
Size: 3755 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2369 2401 2446 2478 4927
Size: 32 135 32 797 32 45 32 2449
Cuts at: 4927 4959 5001 5033 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2449 797 135 45 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2377 2524 3502 3630 3876 669
Size: 988 281 439 147 978 128 246 3079
Fragments arranged by size:
3079 988 978 439 281 246 147 128
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6286
HpaI GTT'AAC
Cuts at: 3876 3876
Size: 6286
KpnI G_GTAC'C
Cuts at: 3611 3611
Size: 6286
MfeI C'AATT_G
Cuts at: 3885 3885
Size: 6286
MluI A'CGCG_T
Cuts at: 6162 6162
Size: 6286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5400 5584 1305
Size: 4095 184 2007
Fragments arranged by size:
4095 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3569 10
Size: 54 1425 343 1737 2727
Fragments arranged by size:
2727 1737 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2137 3149 3449 4244 4603 4762
Size: 648 843 128 1012 300 795 359 159
Cuts at: 4762 518
Size: 2042
Fragments arranged by size:
2042 1012 843 795 648 359 300 159
128
MspA1I CmG'CkG
Cuts at: 1606 2020 2161 3127 3475 3543 3702 4379 5527
Size: 414 141 966 348 68 159 677 1148
Cuts at: 5527 5772 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 966 677 414 348 245 159
141 68
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5734
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6286
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6286
NotI GC'GGCC_GC
Cuts at: 3733 3733
Size: 6286
NspI r_CATG'y
Cuts at: 1132 1229 2245 3154 3644 5189 1132
Size: 97 1016 909 490 1545 2229
Fragments arranged by size:
2229 1545 1016 909 490 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6286
PciI A'CATG_T
Cuts at: 5185 5185
Size: 6286
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2292 3106 1108
Size: 1184 814 4288
Fragments arranged by size:
4288 1184 814
PmeI GTTT'AAAC
Cuts at: 3590 3590
Size: 6286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4355 4387 5868 5900 4355
Size: 32 1481 32 4741
Fragments arranged by size:
4741 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6286
PsiI TTA'TAA
Cuts at: 3856 3856
Size: 6286
PstI C_TGCA'G
Cuts at: 830 2019 2203 3638 830
Size: 1189 184 1435 3478
Fragments arranged by size:
3478 1435 1189 184
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6286
PvuII CAG'CTG
Cuts at: 3127 3543 3127
Size: 416 5870
SalI G'TCGA_C
Cuts at: 1936 2375 3500 3628 1936
Size: 439 1125 128 4594
Fragments arranged by size:
4594 1125 439 128
SapI GCTCTTCn'nnn_
Cuts at: 3557 3557
Size: 6286
SbfI CC_TGCA'GG
Cuts at: 3638 3638
Size: 6286
ScaI AGT'ACT
Cuts at: 4534 6246 4534
Size: 1712 4574
SexAI A'CCwGG_T
Cuts at: 2517 2517
Size: 6286
SfcI C'TryA_G
Cuts at: 826 1045 2015 2199 3634 4769 5450 5641 826
Size: 219 970 184 1435 1135 681 191 1471
Fragments arranged by size:
1471 1435 1135 970 681 219 191 184
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6286
SmaI CCC'GGG
Cuts at: 1872 3215 3613 1872
Size: 1343 398 4545
Fragments arranged by size:
4545 1343 398
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3255 4390 5291 5553 820
Size: 197 926 1312 1135 901 262 1553
Fragments arranged by size:
1553 1312 1135 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4077 493
Size: 3584 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6286
SphI G_CATG'C
Cuts at: 1132 2245 3154 3644 1132
Size: 1113 909 490 3774
Fragments arranged by size:
3774 1113 909 490
SspI AAT'ATT
Cuts at: 5 52 4210 5
Size: 47 4158 2081
Fragments arranged by size:
4158 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6286
StyI C'CwwG_G
Cuts at: 513 1065 2898 3072 3406 3725 513
Size: 552 1833 174 334 319 3074
Fragments arranged by size:
3074 1833 552 334 319 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2974 3452 3497 4313 4330 4483 4668
Size: 1255 1394 478 45 816 17 153 185
Cuts at: 4668 5113 325
Size: 445 1498
Fragments arranged by size:
1498 1394 1255 816 478 445 185 153
45 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4532 6244 96
Size: 274 80 33 51 1443 2555 1712 138
Fragments arranged by size:
2555 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2970 3002 6246 6278 2970
Size: 32 3244 32 2978
Fragments arranged by size:
3244 2978 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2372 2519 1316
Size: 1056 147 5083
Fragments arranged by size:
5083 1056 147
XbaI T'CTAG_A
Cuts at: 3622 3622
Size: 6286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2438 2438
Size: 6286
XhoI C'TCGA_G
Cuts at: 1943 3255 1943
Size: 1312 4974
XmaI C'CCGG_G
Cuts at: 1870 3213 3611 1870
Size: 1343 398 4545
Fragments arranged by size:
4545 1343 398
XmnI GAAnn'nnTTC
Cuts at: 2342 2993 3264 4415 2342
Size: 651 271 1151 4213
Fragments arranged by size:
4213 1151 651 271
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4094 276
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI