Restriction map for
pFN21AB8719
(Circular) (Six-base) MAPSORT of: pFN21AB8719.seq Check: 5445 from: 1 to: 6133
pFN21AB8719 6133 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2722 2778 2722
Size: 56 6077
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3943 278
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3476 1937
Size: 1539 4594
Acc65I G'GTAC_C
Cuts at: 3454 3454
Size: 6133
AclI AA'CG_TT
Cuts at: 1563 4260 4633 1563
Size: 2697 373 3063
Fragments arranged by size:
3063 2697 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2755 3095 4196 5580 5848 1632
Size: 1123 340 1101 1384 268 1917
Fragments arranged by size:
1917 1384 1123 1101 340 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5936
AflIII A'CryG_T
Cuts at: 1203 5032 6009 1203
Size: 3829 977 1327
Fragments arranged by size:
3829 1327 977
AgeI A'CCGG_T
Cuts at: 4964 4964
Size: 6133
AhdI GACnn_n'nnGTC
Cuts at: 4862 4862
Size: 6133
AleI CACnn'nnGTG
Cuts at: 1166 2357 1166
Size: 1191 4942
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3420 1184
Size: 2236 3897
AlwNI CAG_nnn'CTG
Cuts at: 2354 3876 5448 2354
Size: 1522 1572 3039
Fragments arranged by size:
3039 1572 1522
ApaI G_GGCC'C
Cuts at: 2849 3244 2849
Size: 395 5738
ApaLI G'TGCA_C
Cuts at: 4190 5346 4190
Size: 1156 4977
ApoI r'AATT_y
Cuts at: 1493 2668 3442 3668 3820 5929 1493
Size: 1175 774 226 152 2109 1697
Fragments arranged by size:
2109 1697 1175 774 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4687 160
Size: 872 3655 1606
Fragments arranged by size:
3655 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2289 2883 3458 1870
Size: 73 119 227 594 575 4545
Fragments arranged by size:
4545 594 575 227 119 73
BamHI G'GATC_C
Cuts at: 3463 3830 3463
Size: 367 5766
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2790 3331 3454 4909 618
Size: 325 217 627 1003 541 123 1455 1842
Fragments arranged by size:
1842 1455 1003 627 541 325 217 123
BanII G_rGCy'C
Cuts at: 1391 2298 2849 3179 3244 3452 1391
Size: 907 551 330 65 208 4072
Fragments arranged by size:
4072 907 551 330 208 65
BbeI G_GCGC'C
Cuts at: 3335 3335
Size: 6133
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2446 928
Size: 971 547 4615
Fragments arranged by size:
4615 971 547
BbvCI CC'TCA_GC
Cuts at: 2556 2556
Size: 6133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2010 2044 3077 3111 4324 4358 1903
Size: 34 73 34 1033 34 1213 34 3678
Fragments arranged by size:
3678 1213 1033 73 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4025 5235 4025
Size: 1210 4923
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6133
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2853 3089 3248
Size: 107 122 71 631 605 1181 236 159
Cuts at: 3248 4744 136
Size: 1496 1525
Fragments arranged by size:
1525 1496 1181 631 605 236 159 122
107 71
BglII A'GATC_T
Cuts at: 6128 6128
Size: 6133
BlpI GC'TnA_GC
Cuts at: 3550 3550
Size: 6133
Bme1580I G_kGCm'C
Cuts at: 1790 2533 2849 3244 4194 5350 1790
Size: 743 316 395 950 1156 2573
Fragments arranged by size:
2573 1156 950 743 395 316
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6133
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3864 4822 442
Size: 416 53 83 399 24 2447 958 1753
Fragments arranged by size:
2447 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2174 2301 2376 2417 2433 2783 3065
Size: 890 45 127 75 41 16 350 282
Cuts at: 3065 4793 1239
Size: 1728 2579
Fragments arranged by size:
2579 1728 890 350 282 127 75 45
41 16
Bpu10I CC'TnA_GC
Cuts at: 2459 2556 2783 3173 2459
Size: 97 227 390 5419
Fragments arranged by size:
5419 390 227 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3001 3272 4258 5123 5421 3001
Size: 271 986 865 298 3713
Fragments arranged by size:
3713 986 865 298 271
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2639 4796 882
Size: 617 1140 2157 2219
Fragments arranged by size:
2219 2157 1140 617
BsaAI yAC'GTr
Cuts at: 493 1204 3010 3924 493
Size: 711 1806 914 2702
Fragments arranged by size:
2702 1806 914 711
BsaBI GATnn'nnATC
Cuts at: 2255 6127 2255
Size: 3872 2261
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 3332 3940 4322
Size: 53 83 186 936 479 1320 608 382
Cuts at: 4322 4957 275
Size: 635 1451
Fragments arranged by size:
1451 1320 936 635 608 479 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4566 4964 5238 5385 5796 4566
Size: 398 274 147 411 4903
Fragments arranged by size:
4903 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2471 2501 2699 2729 3359
Size: 30 165 30 1068 30 198 30 630
Cuts at: 3359 3389 1178
Size: 30 3922
Fragments arranged by size:
3922 1068 630 198 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2234 2432 2435 2438 2720 1186
Size: 1048 198 3 3 282 4599
Fragments arranged by size:
4599 1048 282 198 3 3
BseYI C'CCAG_C
Cuts at: 2087 2441 2823 3028 3067 3178 3268 5336 5832
Size: 354 382 205 39 111 90 2068 496
Cuts at: 5832 2087
Size: 2388
Fragments arranged by size:
2388 2068 496 382 354 205 111 90
39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5560
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2389 3583 4344 5372 1540
Size: 275 186 388 1194 761 1028 2301
Fragments arranged by size:
2301 1194 1028 761 388 275 186
BsiHKAI G_wGCw'C
Cuts at: 2613 4194 4279 5350 2613
Size: 1581 85 1071 3396
Fragments arranged by size:
3396 1581 1071 85
BsmI GAATG_Cn'
Cuts at: 3644 3737 3644
Size: 93 6040
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 2003
Size: 6133
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2298 2533 2613 2849 3179 3244 3452
Size: 399 508 235 80 236 330 65 208
Cuts at: 3452 4194 4279 5350 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 508 399 330 236 235
208 85 80 65
BspEI T'CCGG_A
Cuts at: 5796 5796
Size: 6133
BspHI T'CATG_A
Cuts at: 4022 5752 4022
Size: 1730 4403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2722 2778 3399 3488 844
Size: 1878 56 621 89 3489
Fragments arranged by size:
3489 1878 621 89 56
BsrBI CCG'CTC
Cuts at: 1394 1823 3064 4020 1394
Size: 429 1241 956 3507
Fragments arranged by size:
3507 1241 956 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4628 4802 65
Size: 1183 3380 174 1396
Fragments arranged by size:
3380 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3328 4777 4964 1700
Size: 110 1518 1449 187 2869
Fragments arranged by size:
2869 1518 1449 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6133
BssSI C'ACGA_G
Cuts at: 2470 4193 5205 2470
Size: 1723 1012 3398
Fragments arranged by size:
3398 1723 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6133
BstBI TT'CG_AA
Cuts at: 4937 4937
Size: 6133
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6133
BstYI r'GATC_y
Cuts at: 1972 3039 3463 3830 4215 4232 5684 5770 5782
Size: 1067 424 367 385 17 1452 86 12
Cuts at: 5782 6128 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1067 424 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2337 2759 2337
Size: 422 5711
BtgI C'CryG_G
Cuts at: 513 1065 2004 2383 513
Size: 552 939 379 4263
Fragments arranged by size:
4263 939 552 379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5394
Fragments arranged by size:
5394 683 56
BtsI GCAGTG_nn'
Cuts at: 3649 4443 4463 3649
Size: 794 20 5319
Fragments arranged by size:
5319 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6098
DraI TTT'AAA
Cuts at: 3437 3437
Size: 6133
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5140 809
Size: 507 3824 1802
Fragments arranged by size:
3824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2386 2511 3081
Size: 54 1425 50 275 18 556 125 570
Cuts at: 3081 3150 3219 3297 3580 4469 6098 8
Size: 69 69 78 283 889 1629 43
Fragments arranged by size:
1629 1425 889 570 556 283 275 125
78 69 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2386 3580 1537
Size: 275 574 1194 4090
Fragments arranged by size:
4090 1194 574 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 2556 2567 4063 1353
Size: 768 435 11 1496 3423
Fragments arranged by size:
3423 1496 768 435 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4720 5094 5240 387
Size: 1064 138 3131 374 146 1280
Fragments arranged by size:
3131 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2174 2301 2376 2417 2433 2755
Size: 393 497 45 127 75 41 16 322
Cuts at: 2755 2783 3065 3095 4196 4793 5580 5848 1239
Size: 28 282 30 1101 597 787 268 1524
Fragments arranged by size:
1524 1101 787 597 497 393 322 282
268 127 75 45 41 30 28 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 2120
Size: 6133
EcoO109I rG'GnC_Cy
Cuts at: 2058 2113 2140 2379 2764 2846 3241 3367 2058
Size: 55 27 239 385 82 395 126 4824
Fragments arranged by size:
4824 395 385 239 126 82 55 27
EcoRI G'AATT_C
Cuts at: 2668 3442 3820 2668
Size: 774 378 4981
Fragments arranged by size:
4981 774 378
FspI TGC'GCA
Cuts at: 4639 4639
Size: 6133
HaeII r_GCGC'y
Cuts at: 1678 3205 3335 5280 1678
Size: 1527 130 1945 2531
Fragments arranged by size:
2531 1945 1527 130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2471 2503 3130 3162 3359
Size: 32 135 32 899 32 627 32 197
Cuts at: 3359 3391 4774 4806 4848 4880 1373
Size: 32 1383 32 42 32 2626
Fragments arranged by size:
2626 1383 899 627 197 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3477 3723 669
Size: 988 281 1539 246 3079
Fragments arranged by size:
3079 1539 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3417 748
Size: 2669 3464
HpaI GTT'AAC
Cuts at: 3723 3723
Size: 6133
KasI G'GCGC_C
Cuts at: 3331 3331
Size: 6133
KpnI G_GTAC'C
Cuts at: 3458 3458
Size: 6133
MfeI C'AATT_G
Cuts at: 3732 3732
Size: 6133
MluI A'CGCG_T
Cuts at: 6009 6009
Size: 6133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2976 5247 5431 1305
Size: 1671 2271 184 2007
Fragments arranged by size:
2271 2007 1671 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2513 3083 3152 10
Size: 54 1425 343 681 570 69 2991
Fragments arranged by size:
2991 1425 681 570 343 69 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 2507 4091 4450 4609 518
Size: 648 1191 150 1584 359 159 2042
Fragments arranged by size:
2042 1584 1191 648 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 2110 2385 2437 2713 3095 3549 4226 5374
Size: 504 275 52 276 382 454 677 1148
Cuts at: 5374 5619 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 504 454 382 276 275
245 52
NarI GG'CG_CC
Cuts at: 3332 3332
Size: 6133
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4642
Fragments arranged by size:
4642 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6133
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6133
NotI GC'GGCC_GC
Cuts at: 3580 3580
Size: 6133
NspI r_CATG'y
Cuts at: 1132 1229 2348 3491 5036 1132
Size: 97 1119 1143 1545 2229
Fragments arranged by size:
2229 1545 1143 1119 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6133
PciI A'CATG_T
Cuts at: 5032 5032
Size: 6133
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6133
PfoI T'CCnGG_A
Cuts at: 2115 2493 3042 2115
Size: 378 549 5206
Fragments arranged by size:
5206 549 378
PmeI GTTT'AAAC
Cuts at: 3437 3437
Size: 6133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 5715 5747 4202
Size: 32 1481 32 4588
Fragments arranged by size:
4588 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2113 2764 3367 2113
Size: 651 603 4879
Fragments arranged by size:
4879 651 603
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6133
PsiI TTA'TAA
Cuts at: 3703 3703
Size: 6133
PspOMI G'GGCC_C
Cuts at: 2845 3240 2845
Size: 395 5738
PspXI vC'TCGA_Gb
Cuts at: 2883 2883
Size: 6133
PstI C_TGCA'G
Cuts at: 830 3312 3399 3485 830
Size: 2482 87 86 3478
Fragments arranged by size:
3478 2482 87 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6133
PvuII CAG'CTG
Cuts at: 2713 2713
Size: 6133
SacII CC_GC'GG
Cuts at: 2386 2386
Size: 6133
SalI G'TCGA_C
Cuts at: 1936 3475 1936
Size: 1539 4594
SanDI GG'GwC_CC
Cuts at: 2764 2764
Size: 6133
SbfI CC_TGCA'GG
Cuts at: 3485 3485
Size: 6133
ScaI AGT'ACT
Cuts at: 4381 6093 4381
Size: 1712 4421
SfcI C'TryA_G
Cuts at: 826 1045 3197 3308 3395 3481 4616 5297 5488
Size: 219 2152 111 87 86 1135 681 191
Cuts at: 5488 826
Size: 1471
Fragments arranged by size:
2152 1471 1135 681 219 191 111 87
86
SfiI GGCCn_nnn'nGGCC
Cuts at: 3089 3248 3089
Size: 159 5974
SfoI GGC'GCC
Cuts at: 3333 3333
Size: 6133
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
SgrAI Cr'CCGG_yG
Cuts at: 1700 3328 1700
Size: 1628 4505
SmaI CCC'GGG
Cuts at: 1872 2291 3460 1872
Size: 419 1169 4545
Fragments arranged by size:
4545 1169 419
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2883 2980 3287 4237 5138 5400
Size: 197 926 940 97 307 950 901 262
Cuts at: 5400 820
Size: 1553
Fragments arranged by size:
1553 950 940 926 901 307 262 197
97
SnaBI TAC'GTA
Cuts at: 493 3924 493
Size: 3431 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6133
SphI G_CATG'C
Cuts at: 1132 2348 3491 1132
Size: 1216 1143 3774
Fragments arranged by size:
3774 1216 1143
SspI AAT'ATT
Cuts at: 5 52 4057 5
Size: 47 4005 2081
Fragments arranged by size:
4005 2081 47
StuI AGG'CCT
Cuts at: 1517 2394 3171 1517
Size: 877 777 4479
Fragments arranged by size:
4479 877 777
StyI C'CwwG_G
Cuts at: 513 1065 2004 2274 2514 3572 513
Size: 552 939 270 240 1058 3074
Fragments arranged by size:
3074 1058 939 552 270 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2879 3296 4160 4177 4330 4515 4960
Size: 1255 1299 417 864 17 153 185 445
Cuts at: 4960 325
Size: 1498
Fragments arranged by size:
1498 1299 1255 864 445 417 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2655 2679 3228
Size: 274 80 33 51 1443 678 24 549
Cuts at: 3228 4379 6091 96
Size: 1151 1712 138
Fragments arranged by size:
1712 1443 1151 678 549 274 138 80
51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6093 6125 6093
Size: 32 6101
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6133
XbaI T'CTAG_A
Cuts at: 3469 3469
Size: 6133
XhoI C'TCGA_G
Cuts at: 1943 2883 1943
Size: 940 5193
XmaI C'CCGG_G
Cuts at: 1870 2289 3458 1870
Size: 419 1169 4545
Fragments arranged by size:
4545 1169 419
XmnI GAAnn'nnTTC
Cuts at: 4262 4262
Size: 6133
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3941 276
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PsrI RsrII
SacI SapI SexAI SrfI SwaI XcmI