Restriction map for
pFN21AB8716
(Circular) (Six-base) MAPSORT of: pFN21AB8716.seq Check: 8848 from: 1 to: 6334
pFN21AB8716 6334 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2384 2399 2384
Size: 15 6319
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4144 278
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3677 1937
Size: 1740 4594
Acc65I G'GTAC_C
Cuts at: 3655 3655
Size: 6334
AclI AA'CG_TT
Cuts at: 1563 4461 4834 1563
Size: 2898 373 3063
Fragments arranged by size:
3063 2898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4397 5781 6049 1632
Size: 2765 1384 268 1917
Fragments arranged by size:
2765 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6137
AflIII A'CryG_T
Cuts at: 1203 5233 6210 1203
Size: 4030 977 1327
Fragments arranged by size:
4030 1327 977
AgeI A'CCGG_T
Cuts at: 5165 5165
Size: 6334
AhdI GACnn_n'nnGTC
Cuts at: 5063 5063
Size: 6334
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6334
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6334
AlwNI CAG_nnn'CTG
Cuts at: 2227 2749 4077 5649 2227
Size: 522 1328 1572 2912
Fragments arranged by size:
2912 1572 1328 522
ApaI G_GGCC'C
Cuts at: 2156 2421 3080 3365 3527 2156
Size: 265 659 285 162 4963
Fragments arranged by size:
4963 659 285 265 162
ApaLI G'TGCA_C
Cuts at: 2338 3152 4391 5547 2338
Size: 814 1239 1156 3125
Fragments arranged by size:
3125 1239 1156 814
ApoI r'AATT_y
Cuts at: 1493 3643 3869 4021 6130 1493
Size: 2150 226 152 2109 1697
Fragments arranged by size:
2150 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4888 160
Size: 872 3856 1606
Fragments arranged by size:
3856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
AvaI C'yCGr_G
Cuts at: 1870 1943 2155 2800 3073 3305 3339 3355 3504
Size: 73 212 645 273 232 34 16 149
Cuts at: 3504 3659 1870
Size: 155 4545
Fragments arranged by size:
4545 645 273 232 212 155 149 73
34 16
BamHI G'GATC_C
Cuts at: 2898 3664 4031 2898
Size: 766 367 5201
Fragments arranged by size:
5201 766 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2610 2849 3170 3196 3280
Size: 325 217 627 823 239 321 26 84
Cuts at: 3280 3655 5110 618
Size: 375 1455 1842
Fragments arranged by size:
1842 1455 823 627 375 325 321 239
217 84 26
BanII G_rGCy'C
Cuts at: 1391 2064 2156 2216 2421 2844 3080 3365 3441
Size: 673 92 60 205 423 236 285 76
Cuts at: 3441 3527 3653 1391
Size: 86 126 4072
Fragments arranged by size:
4072 673 423 285 236 205 126 92
86 76 60
BbeI G_GCGC'C
Cuts at: 2614 2853 3284 2614
Size: 239 431 5664
Fragments arranged by size:
5664 431 239
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3005 928
Size: 971 1106 4257
Fragments arranged by size:
4257 1106 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4525 4559 1903
Size: 34 2588 34 3678
Fragments arranged by size:
3678 2588 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4226 5436 4226
Size: 1210 5124
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6334
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4945 136
Size: 107 122 71 631 605 3273 1525
Fragments arranged by size:
3273 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6329 6329
Size: 6334
BlpI GC'TnA_GC
Cuts at: 2312 3751 2312
Size: 1439 4895
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2156 2325 2342 2421 3080 3156 3201
Size: 226 140 169 17 79 659 76 45
Cuts at: 3201 3365 3527 4395 5551 1790
Size: 164 162 868 1156 2573
Fragments arranged by size:
2573 1156 868 659 226 169 164 162
140 79 76 45 17
BmgBI CAC'GTC
Cuts at: 1318 3151 3373 3544 1318
Size: 1833 222 171 4108
Fragments arranged by size:
4108 1833 222 171
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4065 5023 442
Size: 416 53 83 399 24 2648 958 1753
Fragments arranged by size:
2648 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2264 4994 1239
Size: 1025 2730 2579
Fragments arranged by size:
2730 2579 1025
Bpu10I CC'TnA_GC
Cuts at: 3452 3452
Size: 6334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4459 5324 5622 4459
Size: 865 298 5171
Fragments arranged by size:
5171 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4997 882
Size: 617 3498 2219
Fragments arranged by size:
3498 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2011 2296 4125 493
Size: 711 807 285 1829 2702
Fragments arranged by size:
2702 1829 807 711 285
BsaBI GATnn'nnATC
Cuts at: 3584 6328 3584
Size: 2744 3590
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2611 2778 2850 3281
Size: 53 83 186 936 1078 167 72 431
Cuts at: 3281 4141 4523 5158 275
Size: 860 382 635 1451
Fragments arranged by size:
1451 1078 936 860 635 431 382 186
167 83 72 53
BsaWI w'CCGG_w
Cuts at: 4767 5165 5439 5586 5997 4767
Size: 398 274 147 411 5104
Fragments arranged by size:
5104 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6109
Fragments arranged by size:
6109 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3524 3527 1186
Size: 2338 3 3993
Fragments arranged by size:
3993 2338 3
BseYI C'CCAG_C
Cuts at: 2020 2025 2383 2822 3265 5537 6033 2020
Size: 5 358 439 443 2272 496 2321
Fragments arranged by size:
2321 2272 496 443 439 358 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2561 2828 3464 1163
Size: 573 825 267 636 4033
Fragments arranged by size:
4033 825 636 573 267
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2109 2257 2879 3615 3784 4545
Size: 275 186 108 148 622 736 169 761
Cuts at: 4545 5573 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 736 622 275 186 169
148 108
BsiHKAI G_wGCw'C
Cuts at: 2064 2342 3156 4395 4480 5551 2064
Size: 278 814 1239 85 1071 2847
Fragments arranged by size:
2847 1239 1071 814 278 85
BsmI GAATG_Cn'
Cuts at: 3845 3938 3845
Size: 93 6241
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2064 2156 2216 2325 2342 2421
Size: 399 226 48 92 60 109 17 79
Cuts at: 2421 2844 3080 3156 3201 3365 3441 3527 3653
Size: 423 236 76 45 164 76 86 126
Cuts at: 3653 4395 4480 5551 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 423 399 236 226 164
126 109 92 86 85 79 76 76
60 48 45 17
BspEI T'CCGG_A
Cuts at: 5997 5997
Size: 6334
BspHI T'CATG_A
Cuts at: 2502 4223 5953 2502
Size: 1721 1730 2883
Fragments arranged by size:
2883 1730 1721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2384 2399 3689 844
Size: 1540 15 1290 3489
Fragments arranged by size:
3489 1540 1290 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2616 3039 3301 3378 4221 1394
Size: 429 793 423 262 77 843 3507
Fragments arranged by size:
3507 843 793 429 423 262 77
BsrDI GCAATG_nn'
Cuts at: 65 1248 4829 5003 65
Size: 1183 3581 174 1396
Fragments arranged by size:
3581 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2601 2688 4978 5165 1700
Size: 110 791 87 2290 187 2869
Fragments arranged by size:
2869 2290 791 187 110 87
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6334
BssHII G'CGCG_C
Cuts at: 1925 2118 2379 2990 3131 1925
Size: 193 261 611 141 5128
Fragments arranged by size:
5128 611 261 193 141
BssSI C'ACGA_G
Cuts at: 4394 5406 4394
Size: 1012 5322
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6334
BstBI TT'CG_AA
Cuts at: 5138 5138
Size: 6334
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6334
BstYI r'GATC_y
Cuts at: 1972 2139 2898 3579 3664 4031 4416 4433 5885
Size: 167 759 681 85 367 385 17 1452
Cuts at: 5885 5971 5983 6329 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 759 681 385 367 346 167
86 85 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2251 2421 2787 2814 2865 3006 513
Size: 552 1186 170 366 27 51 141 3841
Fragments arranged by size:
3841 1186 552 366 170 141 51 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2455 3541 3571 487
Size: 683 56 1229 1086 30 3250
Fragments arranged by size:
3250 1229 1086 683 56 30
BtsI GCAGTG_nn'
Cuts at: 2612 3850 4644 4664 2612
Size: 1238 794 20 4282
Fragments arranged by size:
4282 1238 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6299
DraI TTT'AAA
Cuts at: 3638 3638
Size: 6334
DraIII CAC_nnn'GTG
Cuts at: 2584 2584
Size: 6334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2735 5341 809
Size: 507 1419 2606 1802
Fragments arranged by size:
2606 1802 1419 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 2254 2271
Size: 54 1425 50 275 18 276 148 17
Cuts at: 2271 2643 2876 2943 3003 3781 4670 6299 8
Size: 372 233 67 60 778 889 1629 43
Fragments arranged by size:
1629 1425 889 778 372 276 275 233
148 67 60 54 50 43 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 2106 2254 2876 3781 1537
Size: 275 294 148 622 905 4090
Fragments arranged by size:
4090 905 622 294 275 148
EarI CTCTTCn'nnn_
Cuts at: 1353 2204 2543 4264 1353
Size: 851 339 1721 3423
Fragments arranged by size:
3423 1721 851 339
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2987 3031 3075 3266 3449 4921
Size: 1064 138 1398 44 44 191 183 1472
Cuts at: 4921 5295 5441 387
Size: 374 146 1280
Fragments arranged by size:
1472 1398 1280 1064 374 191 183 146
138 44 44
EcoICRI GAG'CTC
Cuts at: 2062 2062
Size: 6334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 4397 4994 5781 6049 1239
Size: 393 632 2133 597 787 268 1524
Fragments arranged by size:
2133 1524 787 632 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2075 3245 3428 3536 2075
Size: 1170 183 108 4873
Fragments arranged by size:
4873 1170 183 108
EcoO109I rG'GnC_Cy
Cuts at: 2152 2417 3077 3361 3523 3524 3550 2152
Size: 265 660 284 162 1 26 4936
Fragments arranged by size:
4936 660 284 265 162 26 1
EcoRI G'AATT_C
Cuts at: 3643 4021 3643
Size: 378 5956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 6334
FspI TGC'GCA
Cuts at: 4840 4840
Size: 6334
HaeII r_GCGC'y
Cuts at: 1678 2251 2319 2614 2759 2829 2853 3284 5481
Size: 573 68 295 145 70 24 431 2197
Cuts at: 5481 1678
Size: 2531
Fragments arranged by size:
2531 2197 573 431 295 145 70 68
24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4975 5007 5049 5081 1373
Size: 32 135 32 3403 32 42 32 2626
Fragments arranged by size:
3403 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2524 3678 3924 669
Size: 988 281 586 1154 246 3079
Fragments arranged by size:
3079 1154 988 586 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6334
HpaI GTT'AAC
Cuts at: 3924 3924
Size: 6334
KasI G'GCGC_C
Cuts at: 2610 2849 3280 2610
Size: 239 431 5664
Fragments arranged by size:
5664 431 239
KpnI G_GTAC'C
Cuts at: 3659 3659
Size: 6334
MfeI C'AATT_G
Cuts at: 3933 3933
Size: 6334
MluI A'CGCG_T
Cuts at: 6210 6210
Size: 6334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2793 5448 5632 1305
Size: 1488 2655 184 2007
Fragments arranged by size:
2655 2007 1488 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2273 2645 2945 10
Size: 54 1425 343 441 372 300 3399
Fragments arranged by size:
3399 1425 441 372 343 300 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2954 4292 4651 4810 518
Size: 648 843 945 1338 359 159 2042
Fragments arranged by size:
2042 1338 945 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2253 2423 2746 2941 3028 3475 3750 4427
Size: 647 170 323 195 87 447 275 677
Cuts at: 4427 5575 5820 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 647 447 323 275 245
195 170 87
NaeI GCC'GGC
Cuts at: 2603 2603
Size: 6334
NarI GG'CG_CC
Cuts at: 2611 2850 3281 2611
Size: 239 431 5664
Fragments arranged by size:
5664 431 239
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5782
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6334
NgoMIV G'CCGG_C
Cuts at: 2601 2601
Size: 6334
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6334
NotI GC'GGCC_GC
Cuts at: 2254 2876 3781 2254
Size: 622 905 4807
Fragments arranged by size:
4807 905 622
NspI r_CATG'y
Cuts at: 1132 1229 3692 5237 1132
Size: 97 2463 1545 2229
Fragments arranged by size:
2463 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6334
PciI A'CATG_T
Cuts at: 5233 5233
Size: 6334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6334
PfoI T'CCnGG_A
Cuts at: 3218 3218
Size: 6334
PmeI GTTT'AAAC
Cuts at: 3638 3638
Size: 6334
PmlI CAC'GTG
Cuts at: 2296 2296
Size: 6334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 5916 5948 4403
Size: 32 1481 32 4789
Fragments arranged by size:
4789 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3550 3550
Size: 6334
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6334
PsiI TTA'TAA
Cuts at: 3904 3904
Size: 6334
PspOMI G'GGCC_C
Cuts at: 2152 2417 3076 3361 3523 2152
Size: 265 659 285 162 4963
Fragments arranged by size:
4963 659 285 265 162
PstI C_TGCA'G
Cuts at: 830 2079 2463 3686 830
Size: 1249 384 1223 3478
Fragments arranged by size:
3478 1249 1223 384
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6334
PvuII CAG'CTG
Cuts at: 2746 2746
Size: 6334
SacI G_AGCT'C
Cuts at: 2064 2064
Size: 6334
SacII CC_GC'GG
Cuts at: 2254 2424 2254
Size: 170 6164
SalI G'TCGA_C
Cuts at: 1936 3676 1936
Size: 1740 4594
SapI GCTCTTCn'nnn_
Cuts at: 2204 2204
Size: 6334
SbfI CC_TGCA'GG
Cuts at: 3686 3686
Size: 6334
ScaI AGT'ACT
Cuts at: 3622 4582 6294 3622
Size: 960 1712 3662
Fragments arranged by size:
3662 1712 960
SfcI C'TryA_G
Cuts at: 826 1045 2075 2459 3682 4817 5498 5689 826
Size: 219 1030 384 1223 1135 681 191 1471
Fragments arranged by size:
1471 1223 1135 1030 681 384 219 191
SfoI GGC'GCC
Cuts at: 2612 2851 3282 2612
Size: 239 431 5664
Fragments arranged by size:
5664 431 239
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6334
SmaI CCC'GGG
Cuts at: 1872 2157 3075 3341 3506 3661 1872
Size: 285 918 266 165 155 4545
Fragments arranged by size:
4545 918 285 266 165 155
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4438 5339 5601 820
Size: 197 926 2495 901 262 1553
Fragments arranged by size:
2495 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4125 493
Size: 3632 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6334
SphI G_CATG'C
Cuts at: 1132 3692 1132
Size: 2560 3774
SrfI GCCC'GGGC
Cuts at: 2157 3341 2157
Size: 1184 5150
SspI AAT'ATT
Cuts at: 5 52 4258 5
Size: 47 4206 2081
Fragments arranged by size:
4206 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6334
StyI C'CwwG_G
Cuts at: 513 1065 2148 3183 3773 513
Size: 552 1083 1035 590 3074
Fragments arranged by size:
3074 1083 1035 590 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4361 4378 4531 4716 5161 325
Size: 1255 2781 17 153 185 445 1498
Fragments arranged by size:
2781 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3620 4580 6292
Size: 274 80 33 51 1443 1643 960 1712
Cuts at: 6292 96
Size: 138
Fragments arranged by size:
1712 1643 1443 960 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3564 3596 6294 6326 3564
Size: 32 2698 32 3572
Fragments arranged by size:
3572 2698 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6334
XbaI T'CTAG_A
Cuts at: 3670 3670
Size: 6334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2281 2953 2281
Size: 672 5662
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6334
XmaI C'CCGG_G
Cuts at: 1870 2155 3073 3339 3504 3659 1870
Size: 285 918 266 165 155 4545
Fragments arranged by size:
4545 918 285 266 165 155
XmnI GAAnn'nnTTC
Cuts at: 2228 2369 4463 2228
Size: 141 2094 4099
Fragments arranged by size:
4099 2094 141
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4142 276
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI
NruI NsiI PacI PspXI PsrI RsrII SanDI SexAI
SfiI SwaI