Restriction map for
pFN21AB8709
(Circular) (Six-base) MAPSORT of: pFN21AB8709.seq Check: 3518 from: 1 to: 5920
pFN21AB8709 5920 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2346 2346
Size: 5920
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3119 3730 278
Size: 53 83 186 936 1583 611 2468
Fragments arranged by size:
2468 1583 936 611 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2553 3263 1937
Size: 616 710 4594
Fragments arranged by size:
4594 710 616
Acc65I G'GTAC_C
Cuts at: 3241 3241
Size: 5920
AclI AA'CG_TT
Cuts at: 1563 2133 4047 4420 1563
Size: 570 1914 373 3063
Fragments arranged by size:
3063 1914 570 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2109 2131 3044 3983 5367 5635 1632
Size: 477 22 913 939 1384 268 1917
Fragments arranged by size:
1917 1384 939 913 477 268 22
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5723
AflIII A'CryG_T
Cuts at: 1203 4819 5796 1203
Size: 3616 977 1327
Fragments arranged by size:
3616 1327 977
AgeI A'CCGG_T
Cuts at: 4751 4751
Size: 5920
AhdI GACnn_n'nnGTC
Cuts at: 4649 4649
Size: 5920
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5920
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5920
AlwNI CAG_nnn'CTG
Cuts at: 2281 2338 3663 5235 2281
Size: 57 1325 1572 2966
Fragments arranged by size:
2966 1572 1325 57
ApaI G_GGCC'C
Cuts at: 2266 2266
Size: 5920
ApaLI G'TGCA_C
Cuts at: 3977 5133 3977
Size: 1156 4764
ApoI r'AATT_y
Cuts at: 1493 2479 2992 3229 3455 3607 5716 1493
Size: 986 513 237 226 152 2109 1697
Fragments arranged by size:
2109 1697 986 513 237 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4474 160
Size: 872 3442 1606
Fragments arranged by size:
3442 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5920
AvaI C'yCGr_G
Cuts at: 1870 1943 3245 1870
Size: 73 1302 4545
Fragments arranged by size:
4545 1302 73
BamHI G'GATC_C
Cuts at: 2074 3250 3617 2074
Size: 1176 367 4377
Fragments arranged by size:
4377 1176 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2243 2695 3241 4696 618
Size: 325 217 627 456 452 546 1455 1842
Fragments arranged by size:
1842 1455 627 546 456 452 325 217
BanII G_rGCy'C
Cuts at: 1391 2266 2748 3239 1391
Size: 875 482 491 4072
Fragments arranged by size:
4072 875 491 482
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2021 2785 928
Size: 971 122 764 4063
Fragments arranged by size:
4063 971 764 122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4111 4145 1903
Size: 34 50 34 2090 34 3678
Fragments arranged by size:
3678 2090 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3812 5022 3812
Size: 1210 4710
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5920
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4531 136
Size: 107 122 71 631 605 2859 1525
Fragments arranged by size:
2859 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5915 5915
Size: 5920
BlpI GC'TnA_GC
Cuts at: 3337 3337
Size: 5920
Bme1580I G_kGCm'C
Cuts at: 1790 2246 2266 2700 3981 5137 1790
Size: 456 20 434 1281 1156 2573
Fragments arranged by size:
2573 1281 1156 456 434 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5920
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2500 3105 3209
Size: 416 53 83 399 24 1083 605 104
Cuts at: 3209 3651 4609 442
Size: 442 958 1753
Fragments arranged by size:
1753 1083 958 605 442 416 399 104
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5920
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 5920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2258 2435 2444 2946 2972 3202 4580 1239
Size: 1019 177 9 502 26 230 1378 2579
Fragments arranged by size:
2579 1378 1019 502 230 177 26 9
Bpu10I CC'TnA_GC
Cuts at: 2224 2224
Size: 5920
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2194 4045 4910 5208 2194
Size: 1851 865 298 2906
Fragments arranged by size:
2906 1851 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4583 882
Size: 617 3084 2219
Fragments arranged by size:
3084 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2776 3711 493
Size: 711 1572 935 2702
Fragments arranged by size:
2702 1572 935 711
BsaBI GATnn'nnATC
Cuts at: 5914 5914
Size: 5920
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3015 3116 3727 4109
Size: 53 83 186 936 1482 101 611 382
Cuts at: 4109 4744 275
Size: 635 1451
Fragments arranged by size:
1482 1451 936 635 611 382 186 101
83 53
BsaWI w'CCGG_w
Cuts at: 2806 4353 4751 5025 5172 5583 2806
Size: 1547 398 274 147 411 3143
Fragments arranged by size:
3143 1547 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5695
Fragments arranged by size:
5695 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2003 2672 3083 1186
Size: 817 669 411 4023
Fragments arranged by size:
4023 817 669 411
BseYI C'CCAG_C
Cuts at: 2265 2578 3018 5123 5619 2265
Size: 313 440 2105 496 2566
Fragments arranged by size:
2566 2105 496 440 313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2324 2783 2823 1163
Size: 573 588 459 40 4260
Fragments arranged by size:
4260 588 573 459 40
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3370 4131 5159 1540
Size: 275 186 1369 761 1028 2301
Fragments arranged by size:
2301 1369 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2720 2748 3981 4066 5137 2720
Size: 28 1233 85 1071 3503
Fragments arranged by size:
3503 1233 1071 85 28
BsmI GAATG_Cn'
Cuts at: 3431 3524 3431
Size: 93 5827
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2246 2266 2700 2720 2748 3239 3981
Size: 399 456 20 434 20 28 491 742
Cuts at: 3981 4066 5137 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 491 456 434 399 85
28 20 20
BspEI T'CCGG_A
Cuts at: 2806 5583 2806
Size: 2777 3143
BspHI T'CATG_A
Cuts at: 2734 3159 3809 5539 2734
Size: 425 650 1730 3115
Fragments arranged by size:
3115 1730 650 425
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2346 3275 844
Size: 1502 929 3489
Fragments arranged by size:
3489 1502 929
BsrBI CCG'CTC
Cuts at: 1394 1823 3807 1394
Size: 429 1984 3507
Fragments arranged by size:
3507 1984 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2395 2788 2964 4415 4589 65
Size: 1183 1147 393 176 1451 174 1396
Fragments arranged by size:
1451 1396 1183 1147 393 176 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4564 4751 1700
Size: 110 2754 187 2869
Fragments arranged by size:
2869 2754 187 110
BsrGI T'GTAC_A
Cuts at: 96 2019 2901 96
Size: 1923 882 3115
Fragments arranged by size:
3115 1923 882
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5920
BssSI C'ACGA_G
Cuts at: 3980 4992 3980
Size: 1012 4908
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5920
BstBI TT'CG_AA
Cuts at: 4724 4724
Size: 5920
BstEII G'GTnAC_C
Cuts at: 1184 2914 3100 1184
Size: 1730 186 4004
Fragments arranged by size:
4004 1730 186
BstYI r'GATC_y
Cuts at: 1972 2074 2316 3250 3617 4002 4019 5471 5557
Size: 102 242 934 367 385 17 1452 86
Cuts at: 5557 5569 5915 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 934 385 367 346 242 102
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2070 2217 2382 513
Size: 552 1005 147 165 4051
Fragments arranged by size:
4051 1005 552 165 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2818 487
Size: 683 56 1592 3589
Fragments arranged by size:
3589 1592 683 56
BtsI GCAGTG_nn'
Cuts at: 3436 4230 4250 3436
Size: 794 20 5106
Fragments arranged by size:
5106 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5920
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5885
DraI TTT'AAA
Cuts at: 3224 3224
Size: 5920
DraIII CAC_nnn'GTG
Cuts at: 2167 2167
Size: 5920
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4927 809
Size: 507 3611 1802
Fragments arranged by size:
3611 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2251 3367 4256
Size: 54 1425 50 275 18 421 1116 889
Cuts at: 4256 5885 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1116 889 421 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3367 1537
Size: 275 1555 4090
Fragments arranged by size:
4090 1555 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3850 1353
Size: 2497 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4507 4881 5027 387
Size: 1064 138 2918 374 146 1280
Fragments arranged by size:
2918 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2746 2746
Size: 5920
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2131 2258 2435 2444 2946 2972
Size: 393 477 22 127 177 9 502 26
Cuts at: 2972 3044 3202 3983 4580 5367 5635 1239
Size: 72 158 781 597 787 268 1524
Fragments arranged by size:
1524 787 781 597 502 477 393 268
177 158 127 72 26 22 9
EcoO109I rG'GnC_Cy
Cuts at: 2116 2278 2116
Size: 162 5758
EcoRI G'AATT_C
Cuts at: 3229 3607 3229
Size: 378 5542
FspI TGC'GCA
Cuts at: 4426 4426
Size: 5920
HaeII r_GCGC'y
Cuts at: 1678 5067 1678
Size: 3389 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2092 2124 4561 4593 4635
Size: 32 135 32 520 32 2437 32 42
Cuts at: 4635 4667 1373
Size: 32 2626
Fragments arranged by size:
2626 2437 520 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2137 2554 2599 3264 3510 669
Size: 988 281 199 417 45 665 246 3079
Fragments arranged by size:
3079 988 665 417 281 246 199 45
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5920
HpaI GTT'AAC
Cuts at: 3510 3510
Size: 5920
KpnI G_GTAC'C
Cuts at: 3245 3245
Size: 5920
MfeI C'AATT_G
Cuts at: 3519 3519
Size: 5920
MluI A'CGCG_T
Cuts at: 5796 5796
Size: 5920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5034 5218 1305
Size: 3729 184 2007
Fragments arranged by size:
3729 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2253 10
Size: 54 1425 343 421 3677
Fragments arranged by size:
3677 1425 421 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2216 2454 3158 3878 4237 4396 518
Size: 648 1050 238 704 720 359 159 2042
Fragments arranged by size:
2042 1050 720 704 648 359 238 159
MspA1I CmG'CkG
Cuts at: 1606 2049 2758 3022 3086 3336 4013 5161 5406
Size: 443 709 264 64 250 677 1148 245
Cuts at: 5406 1606
Size: 2120
Fragments arranged by size:
2120 1148 709 677 443 264 250 245
64
NcoI C'CATG_G
Cuts at: 513 1065 2070 2217 2382 513
Size: 552 1005 147 165 4051
Fragments arranged by size:
4051 1005 552 165 147
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5920
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5920
NotI GC'GGCC_GC
Cuts at: 3367 3367
Size: 5920
NspI r_CATG'y
Cuts at: 1132 1229 3278 4823 1132
Size: 97 2049 1545 2229
Fragments arranged by size:
2229 2049 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2239 1348
Size: 891 5029
PciI A'CATG_T
Cuts at: 4819 4819
Size: 5920
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2071 2281 1108
Size: 963 210 4747
Fragments arranged by size:
4747 963 210
PfoI T'CCnGG_A
Cuts at: 2484 2484
Size: 5920
PmeI GTTT'AAAC
Cuts at: 3224 3224
Size: 5920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3989 4021 5502 5534 3989
Size: 32 1481 32 4375
Fragments arranged by size:
4375 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5920
PsiI TTA'TAA
Cuts at: 3490 3490
Size: 5920
PspOMI G'GGCC_C
Cuts at: 2262 2262
Size: 5920
PstI C_TGCA'G
Cuts at: 830 3272 830
Size: 2442 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5920
PvuII CAG'CTG
Cuts at: 2758 3022 3086 2758
Size: 264 64 5592
Fragments arranged by size:
5592 264 64
SacI G_AGCT'C
Cuts at: 2748 2748
Size: 5920
SalI G'TCGA_C
Cuts at: 1936 2552 3262 1936
Size: 616 710 4594
Fragments arranged by size:
4594 710 616
SbfI CC_TGCA'GG
Cuts at: 3272 3272
Size: 5920
ScaI AGT'ACT
Cuts at: 4168 5880 4168
Size: 1712 4208
SfcI C'TryA_G
Cuts at: 826 1045 3268 4403 5084 5275 826
Size: 219 2223 1135 681 191 1471
Fragments arranged by size:
2223 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5920
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5920
SmaI CCC'GGG
Cuts at: 1872 3247 1872
Size: 1375 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2173 4024 4925 5187 820
Size: 197 926 230 1851 901 262 1553
Fragments arranged by size:
1851 1553 926 901 262 230 197
SnaBI TAC'GTA
Cuts at: 493 3711 493
Size: 3218 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5920
SphI G_CATG'C
Cuts at: 1132 3278 1132
Size: 2146 3774
SspI AAT'ATT
Cuts at: 5 52 3844 5
Size: 47 3792 2081
Fragments arranged by size:
3792 2081 47
StuI AGG'CCT
Cuts at: 1517 2877 1517
Size: 1360 4560
StyI C'CwwG_G
Cuts at: 513 1065 2070 2217 2382 3359 513
Size: 552 1005 147 165 977 3074
Fragments arranged by size:
3074 1005 977 552 165 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3023 3947 3964 4117 4302 4747 325
Size: 1255 1443 924 17 153 185 445 1498
Fragments arranged by size:
1498 1443 1255 924 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2019 2901 4166
Size: 274 80 33 51 1443 42 882 1265
Cuts at: 4166 5878 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1265 882 274 138 80 51
42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5880 5912 5880
Size: 32 5888
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5920
XbaI T'CTAG_A
Cuts at: 3256 3256
Size: 5920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2590 2890 2590
Size: 300 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5920
XmaI C'CCGG_G
Cuts at: 1870 3245 1870
Size: 1375 4545
XmnI GAAnn'nnTTC
Cuts at: 2192 4049 2192
Size: 1857 4063
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3117 3728 276
Size: 53 83 186 936 1583 611 2468
Fragments arranged by size:
2468 1583 936 611 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI