Restriction map for
pFN21AB8708
(Circular) (Six-base) MAPSORT of: pFN21AB8708.seq Check: 8312 from: 1 to: 6628
pFN21AB8708 6628 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2483 2483
Size: 6628
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4438 278
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3971 1937
Size: 2034 4594
Acc65I G'GTAC_C
Cuts at: 2945 3949 2945
Size: 1004 5624
AclI AA'CG_TT
Cuts at: 1563 4755 5128 1563
Size: 3192 373 3063
Fragments arranged by size:
3192 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3044 3612 4691 6075 6343 1632
Size: 1412 568 1079 1384 268 1917
Fragments arranged by size:
1917 1412 1384 1079 568 268
AfeI AGC'GCT
Cuts at: 2970 2970
Size: 6628
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6431
AflIII A'CryG_T
Cuts at: 1203 2590 5527 6504 1203
Size: 1387 2937 977 1327
Fragments arranged by size:
2937 1387 1327 977
AgeI A'CCGG_T
Cuts at: 5459 5459
Size: 6628
AhdI GACnn_n'nnGTC
Cuts at: 5357 5357
Size: 6628
AleI CACnn'nnGTG
Cuts at: 1166 2694 1166
Size: 1528 5100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6628
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2577 2609 2577
Size: 32 6596
AlwNI CAG_nnn'CTG
Cuts at: 3055 3181 4371 5943 3055
Size: 126 1190 1572 3740
Fragments arranged by size:
3740 1572 1190 126
ApaLI G'TGCA_C
Cuts at: 4685 5841 4685
Size: 1156 5472
ApoI r'AATT_y
Cuts at: 1493 2240 3937 4163 4315 6424 1493
Size: 747 1697 226 152 2109 1697
Fragments arranged by size:
2109 1697 1697 747 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2295 5182 160
Size: 872 1263 2887 1606
Fragments arranged by size:
2887 1606 1263 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
AvaI C'yCGr_G
Cuts at: 1870 1943 3317 3953 1870
Size: 73 1374 636 4545
Fragments arranged by size:
4545 1374 636 73
BamHI G'GATC_C
Cuts at: 3958 4325 3958
Size: 367 6261
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2945 3949 5404 618
Size: 325 217 627 258 900 1004 1455 1842
Fragments arranged by size:
1842 1455 1004 900 627 325 258 217
BanII G_rGCy'C
Cuts at: 1391 2538 2911 3029 3741 3947 1391
Size: 1147 373 118 712 206 4072
Fragments arranged by size:
4072 1147 712 373 206 118
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5657
BbvCI CC'TCA_GC
Cuts at: 2403 2403
Size: 6628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4819 4853 1903
Size: 34 2882 34 3678
Fragments arranged by size:
3678 2882 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4520 5730 4520
Size: 1210 5418
BclI T'GATC_A
Cuts at: 1548 3787 1548
Size: 2239 4389
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3892 5239 136
Size: 107 122 71 631 605 2220 1347 1525
Fragments arranged by size:
2220 1525 1347 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6623 6623
Size: 6628
BlpI GC'TnA_GC
Cuts at: 4045 4045
Size: 6628
Bme1580I G_kGCm'C
Cuts at: 1790 2048 4689 5845 1790
Size: 258 2641 1156 2573
Fragments arranged by size:
2641 2573 1156 258
BmgBI CAC'GTC
Cuts at: 1318 3080 3196 1318
Size: 1762 116 4750
Fragments arranged by size:
4750 1762 116
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3867 4359 5317
Size: 416 53 83 399 24 2450 492 958
Cuts at: 5317 442
Size: 1753
Fragments arranged by size:
2450 1753 958 492 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6628
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 6628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2972 3239 3390 3773 5288 1239
Size: 1733 267 151 383 1515 2579
Fragments arranged by size:
2579 1733 1515 383 267 151
Bpu10I CC'TnA_GC
Cuts at: 2403 3539 2403
Size: 1136 5492
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4753 5618 5916 4753
Size: 865 298 5465
Fragments arranged by size:
5465 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5291 882
Size: 617 3792 2219
Fragments arranged by size:
3792 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2134 4419 493
Size: 711 930 2285 2702
Fragments arranged by size:
2702 2285 930 711
BsaBI GATnn'nnATC
Cuts at: 3515 6622 3515
Size: 3107 3521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4435 4817 5452 275
Size: 53 83 186 936 2902 382 635 1451
Fragments arranged by size:
2902 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5061 5459 5733 5880 6291 5061
Size: 398 274 147 411 5398
Fragments arranged by size:
5398 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3800 3830 1178
Size: 30 165 30 2397 30 3976
Fragments arranged by size:
3976 2397 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2753 3563 1186
Size: 1567 810 4251
Fragments arranged by size:
4251 1567 810
BseYI C'CCAG_C
Cuts at: 2126 2678 3227 3376 5831 6327 2126
Size: 552 549 149 2455 496 2427
Fragments arranged by size:
2455 2427 552 549 496 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2949 3068 1163
Size: 573 1213 119 4723
Fragments arranged by size:
4723 1213 573 119
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4078 4839 5867 1540
Size: 275 186 2077 761 1028 2301
Fragments arranged by size:
2301 2077 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2911 3494 3741 4689 4774 5845 2911
Size: 583 247 948 85 1071 3694
Fragments arranged by size:
3694 1071 948 583 247 85
BsmI GAATG_Cn'
Cuts at: 3634 4139 4232 3634
Size: 505 93 6030
Fragments arranged by size:
6030 505 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3215 3215
Size: 6628
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2538 2911 3029 3494 3741 3947
Size: 399 258 490 373 118 465 247 206
Cuts at: 3947 4689 4774 5845 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 490 465 399 373 258
247 206 118 85
BspEI T'CCGG_A
Cuts at: 6291 6291
Size: 6628
BspHI T'CATG_A
Cuts at: 4517 6247 4517
Size: 1730 4898
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2483 3189 3381 3983 844
Size: 1639 706 192 602 3489
Fragments arranged by size:
3489 1639 706 602 192
BsrBI CCG'CTC
Cuts at: 1394 1823 2155 4515 1394
Size: 429 332 2360 3507
Fragments arranged by size:
3507 2360 429 332
BsrDI GCAATG_nn'
Cuts at: 65 1248 2196 2550 5123 5297 65
Size: 1183 948 354 2573 174 1396
Fragments arranged by size:
2573 1396 1183 948 354 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3494 5272 5459 1700
Size: 110 1684 1778 187 2869
Fragments arranged by size:
2869 1778 1684 187 110
BsrGI T'GTAC_A
Cuts at: 96 2257 96
Size: 2161 4467
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6628
BssSI C'ACGA_G
Cuts at: 4688 5700 4688
Size: 1012 5616
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2808 3055 3629 794
Size: 2014 247 574 3793
Fragments arranged by size:
3793 2014 574 247
BstBI TT'CG_AA
Cuts at: 5432 5432
Size: 6628
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6628
BstXI CCAn_nnnn'nTGG
Cuts at: 2134 3151 2134
Size: 1017 5611
BstYI r'GATC_y
Cuts at: 1972 3958 4325 4710 4727 6179 6265 6277 6623
Size: 1986 367 385 17 1452 86 12 346
Cuts at: 6623 1972
Size: 1977
Fragments arranged by size:
1986 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2358 2358
Size: 6628
BtgI C'CryG_G
Cuts at: 513 1065 2004 2538 3480 513
Size: 552 939 534 942 3661
Fragments arranged by size:
3661 942 939 552 534
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2080 3502 487
Size: 683 56 854 1422 3613
Fragments arranged by size:
3613 1422 854 683 56
BtsI GCAGTG_nn'
Cuts at: 2442 2673 4144 4938 4958 2442
Size: 231 1471 794 20 4112
Fragments arranged by size:
4112 1471 794 231 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6628
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6593
DraI TTT'AAA
Cuts at: 3932 3932
Size: 6628
DraIII CAC_nnn'GTG
Cuts at: 2734 2734
Size: 6628
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2833 5635 809
Size: 507 1517 2802 1802
Fragments arranged by size:
2802 1802 1517 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 4075 4964
Size: 54 1425 50 275 18 342 1903 889
Cuts at: 4964 6593 8
Size: 1629 43
Fragments arranged by size:
1903 1629 1425 889 342 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4075 1537
Size: 275 2263 4090
Fragments arranged by size:
4090 2263 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2374 2913 3653 4558 1353
Size: 1021 539 740 905 3423
Fragments arranged by size:
3423 1021 905 740 539
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5215 5589 5735 387
Size: 1064 138 3626 374 146 1280
Fragments arranged by size:
3626 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2909 3739 2909
Size: 830 5798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2972 3044 3239 3390 3612 3773 4691
Size: 393 1340 72 195 151 222 161 918
Cuts at: 4691 5288 6075 6343 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1340 918 787 597 393 268 222
195 161 151 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2812 3542 3900 2812
Size: 730 358 5540
Fragments arranged by size:
5540 730 358
EcoO109I rG'GnC_Cy
Cuts at: 2826 3178 3905 2826
Size: 352 727 5549
Fragments arranged by size:
5549 727 352
EcoRI G'AATT_C
Cuts at: 3937 4315 3937
Size: 378 6250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3649 3649
Size: 6628
FspI TGC'GCA
Cuts at: 2801 5134 2801
Size: 2333 4295
HaeII r_GCGC'y
Cuts at: 1678 2972 5775 1678
Size: 1294 2803 2531
Fragments arranged by size:
2803 2531 1294
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2590 2622 3069 3101 5269
Size: 32 135 32 1018 32 447 32 2168
Cuts at: 5269 5301 5343 5375 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2168 1018 447 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3972 4218 669
Size: 988 281 2034 246 3079
Fragments arranged by size:
3079 2034 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2346 748
Size: 1598 5030
HpaI GTT'AAC
Cuts at: 4218 4218
Size: 6628
KpnI G_GTAC'C
Cuts at: 2949 3953 2949
Size: 1004 5624
MfeI C'AATT_G
Cuts at: 3912 4227 3912
Size: 315 6313
MluI A'CGCG_T
Cuts at: 6504 6504
Size: 6628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5742 5926 1305
Size: 4437 184 2007
Fragments arranged by size:
4437 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4806
Fragments arranged by size:
4806 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2694 3149 3479 4586 4945 5104 518
Size: 648 1528 455 330 1107 359 159 2042
Fragments arranged by size:
2042 1528 1107 648 455 359 330 159
MspA1I CmG'CkG
Cuts at: 1606 3052 4044 4721 5869 6114 1606
Size: 1446 992 677 1148 245 2120
Fragments arranged by size:
2120 1446 1148 992 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3480 513
Size: 552 939 1476 3661
Fragments arranged by size:
3661 1476 939 552
NdeI CA'TA_TG
Cuts at: 387 3121 387
Size: 2734 3894
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6628
NotI GC'GGCC_GC
Cuts at: 4075 4075
Size: 6628
NspI r_CATG'y
Cuts at: 1132 1229 2594 3986 5531 1132
Size: 97 1365 1392 1545 2229
Fragments arranged by size:
2229 1545 1392 1365 97
PasI CC'CwG_GG
Cuts at: 1348 3211 3414 1348
Size: 1863 203 4562
Fragments arranged by size:
4562 1863 203
PciI A'CATG_T
Cuts at: 2590 5527 2590
Size: 2937 3691
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2734 1108
Size: 1626 5002
PmeI GTTT'AAAC
Cuts at: 3932 3932
Size: 6628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4697 4729 6210 6242 4697
Size: 32 1481 32 5083
Fragments arranged by size:
5083 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2826 3178 2826
Size: 352 6276
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6628
PsiI TTA'TAA
Cuts at: 4198 4198
Size: 6628
PstI C_TGCA'G
Cuts at: 830 3980 830
Size: 3150 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6628
SacI G_AGCT'C
Cuts at: 2911 3741 2911
Size: 830 5798
SalI G'TCGA_C
Cuts at: 1936 3970 1936
Size: 2034 4594
SbfI CC_TGCA'GG
Cuts at: 3980 3980
Size: 6628
ScaI AGT'ACT
Cuts at: 4876 6588 4876
Size: 1712 4916
SexAI A'CCwGG_T
Cuts at: 2181 2181
Size: 6628
SfcI C'TryA_G
Cuts at: 826 1045 2958 3976 5111 5792 5983 826
Size: 219 1913 1018 1135 681 191 1471
Fragments arranged by size:
1913 1471 1135 1018 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6628
SmaI CCC'GGG
Cuts at: 1872 3319 3955 1872
Size: 1447 636 4545
Fragments arranged by size:
4545 1447 636
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4732 5633 5895 820
Size: 197 926 2789 901 262 1553
Fragments arranged by size:
2789 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4419 493
Size: 3926 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6628
SphI G_CATG'C
Cuts at: 1132 3986 1132
Size: 2854 3774
SspI AAT'ATT
Cuts at: 5 52 3720 4552 5
Size: 47 3668 832 2081
Fragments arranged by size:
3668 2081 832 47
StuI AGG'CCT
Cuts at: 1517 2408 3895 1517
Size: 891 1487 4250
Fragments arranged by size:
4250 1487 891
StyI C'CwwG_G
Cuts at: 513 1065 2004 2016 3042 3480 3844 4067 513
Size: 552 939 12 1026 438 364 223 3074
Fragments arranged by size:
3074 1026 939 552 438 364 223 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2600 2684 2858 3427 4655 4672 4825
Size: 1255 1020 84 174 569 1228 17 153
Cuts at: 4825 5010 5455 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1228 1020 569 445 185 174
153 84 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2257 4874 6586
Size: 274 80 33 51 1443 280 2617 1712
Cuts at: 6586 96
Size: 138
Fragments arranged by size:
2617 1712 1443 280 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2499 2531 3800 3832 6588 6620 2499
Size: 32 1269 32 2756 32 2507
Fragments arranged by size:
2756 2507 1269 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2830 1316
Size: 1514 5114
XbaI T'CTAG_A
Cuts at: 3964 3964
Size: 6628
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6628
XmaI C'CCGG_G
Cuts at: 1870 3317 3953 1870
Size: 1447 636 4545
Fragments arranged by size:
4545 1447 636
XmnI GAAnn'nnTTC
Cuts at: 4757 4757
Size: 6628
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4436 276
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI