Restriction map for
pFN21AB8707
(Circular) (Six-base) MAPSORT of: pFN21AB8707.seq Check: 4354 from: 1 to: 6322
pFN21AB8707 6322 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4132 278
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3526 3665 1937
Size: 1589 139 4594
Fragments arranged by size:
4594 1589 139
Acc65I G'GTAC_C
Cuts at: 3643 3643
Size: 6322
AclI AA'CG_TT
Cuts at: 1563 4449 4822 1563
Size: 2886 373 3063
Fragments arranged by size:
3063 2886 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3570 4385 5769 6037 1632
Size: 1938 815 1384 268 1917
Fragments arranged by size:
1938 1917 1384 815 268
AflII C'TTAA_G
Cuts at: 820 1017 2554 820
Size: 197 1537 4588
Fragments arranged by size:
4588 1537 197
AflIII A'CryG_T
Cuts at: 1203 5221 6198 1203
Size: 4018 977 1327
Fragments arranged by size:
4018 1327 977
AgeI A'CCGG_T
Cuts at: 5153 5153
Size: 6322
AhdI GACnn_n'nnGTC
Cuts at: 5051 5051
Size: 6322
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6322
AlwNI CAG_nnn'CTG
Cuts at: 3476 4065 5637 3476
Size: 589 1572 4161
Fragments arranged by size:
4161 1572 589
ApaLI G'TGCA_C
Cuts at: 4379 5535 4379
Size: 1156 5166
ApoI r'AATT_y
Cuts at: 1493 2273 2478 2669 2843 3208 3631 3857 4009
Size: 780 205 191 174 365 423 226 152
Cuts at: 4009 6118 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 780 423 365 226 205 191
174 152
AseI AT'TA_AT
Cuts at: 160 1032 4876 160
Size: 872 3844 1606
Fragments arranged by size:
3844 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
AvaI C'yCGr_G
Cuts at: 1870 1943 3647 1870
Size: 73 1704 4545
Fragments arranged by size:
4545 1704 73
AvrII C'CTAG_G
Cuts at: 2870 2870
Size: 6322
BamHI G'GATC_C
Cuts at: 3652 4019 3652
Size: 367 5955
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3643 5098 618
Size: 325 217 627 1856 1455 1842
Fragments arranged by size:
1856 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3641 1391
Size: 2250 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2085 2997 928
Size: 971 186 912 4253
Fragments arranged by size:
4253 971 912 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2575 2609 4513 4547 1903
Size: 34 638 34 1904 34 3678
Fragments arranged by size:
3678 1904 638 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4214 5424 4214
Size: 1210 5112
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6322
BfrBI ATG'CAT
Cuts at: 2566 2566
Size: 6322
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4933 136
Size: 107 122 71 631 605 3261 1525
Fragments arranged by size:
3261 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6317 6317
Size: 6322
BlpI GC'TnA_GC
Cuts at: 3739 3739
Size: 6322
Bme1580I G_kGCm'C
Cuts at: 1790 4383 5539 1790
Size: 2593 1156 2573
Fragments arranged by size:
2593 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6322
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4053 5011 442
Size: 416 53 83 399 24 2636 958 1753
Fragments arranged by size:
2636 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2769 4982 1239
Size: 1530 2213 2579
Fragments arranged by size:
2579 2213 1530
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4447 5312 5610 4447
Size: 865 298 5159
Fragments arranged by size:
5159 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3525 4985 882
Size: 617 2026 1460 2219
Fragments arranged by size:
2219 2026 1460 617
BsaAI yAC'GTr
Cuts at: 493 1204 3380 4113 493
Size: 711 2176 733 2702
Fragments arranged by size:
2702 2176 733 711
BsaBI GATnn'nnATC
Cuts at: 6316 6316
Size: 6322
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3325 4129 4511 5146
Size: 53 83 186 936 1792 804 382 635
Cuts at: 5146 275
Size: 1451
Fragments arranged by size:
1792 1451 936 804 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3194 4755 5153 5427 5574 5985 3194
Size: 1561 398 274 147 411 3531
Fragments arranged by size:
3531 1561 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2181 2211 1178
Size: 30 165 30 778 30 5289
Fragments arranged by size:
5289 778 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6322
BseYI C'CCAG_C
Cuts at: 5525 6021 5525
Size: 496 5826
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3065 1163
Size: 573 1329 4420
Fragments arranged by size:
4420 1329 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3772 4533 5561 1540
Size: 275 186 1771 761 1028 2301
Fragments arranged by size:
2301 1771 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3411 4383 4468 5539 3411
Size: 972 85 1071 4194
Fragments arranged by size:
4194 1071 972 85
BsiWI C'GTAC_G
Cuts at: 2582 2582
Size: 6322
BsmI GAATG_Cn'
Cuts at: 2639 3833 3926 2639
Size: 1194 93 5035
Fragments arranged by size:
5035 1194 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3316 3316
Size: 6322
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3411 3641 4383 4468 5539 1391
Size: 399 1621 230 742 85 1071 2174
Fragments arranged by size:
2174 1621 1071 742 399 230 85
BspEI T'CCGG_A
Cuts at: 5985 5985
Size: 6322
BspHI T'CATG_A
Cuts at: 2074 2662 4211 5941 2074
Size: 588 1549 1730 2455
Fragments arranged by size:
2455 1730 1549 588
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3037 3677 844
Size: 2193 640 3489
Fragments arranged by size:
3489 2193 640
BsrBI CCG'CTC
Cuts at: 1394 1823 4209 1394
Size: 429 2386 3507
Fragments arranged by size:
3507 2386 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4817 4991 65
Size: 1183 3569 174 1396
Fragments arranged by size:
3569 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4966 5153 1700
Size: 110 3156 187 2869
Fragments arranged by size:
3156 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6322
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6322
BssSI C'ACGA_G
Cuts at: 4382 5394 4382
Size: 1012 5310
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6322
BstBI TT'CG_AA
Cuts at: 5126 5126
Size: 6322
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6322
BstXI CCAn_nnnn'nTGG
Cuts at: 2238 2238
Size: 6322
BstYI r'GATC_y
Cuts at: 1972 3415 3652 4019 4404 4421 5873 5959 5971
Size: 1443 237 367 385 17 1452 86 12
Cuts at: 5971 6317 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1443 385 367 346 237 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2406 2406
Size: 6322
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4831
Fragments arranged by size:
4831 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5583
Fragments arranged by size:
5583 683 56
BtsI GCAGTG_nn'
Cuts at: 3838 4632 4652 3838
Size: 794 20 5508
Fragments arranged by size:
5508 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6322
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6287
DraI TTT'AAA
Cuts at: 2386 3626 2386
Size: 1240 5082
DraIII CAC_nnn'GTG
Cuts at: 3383 3383
Size: 6322
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5329 809
Size: 507 4013 1802
Fragments arranged by size:
4013 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3769 4658 6287
Size: 54 1425 50 275 18 1939 889 1629
Cuts at: 6287 8
Size: 43
Fragments arranged by size:
1939 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3769 1537
Size: 275 1957 4090
Fragments arranged by size:
4090 1957 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2534 2607 3154 3331 4252 1353
Size: 1181 73 547 177 921 3423
Fragments arranged by size:
3423 1181 921 547 177 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4909 5283 5429 387
Size: 1064 138 435 2885 374 146 1280
Fragments arranged by size:
2885 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2769 3570 4385 4982 5769 6037 1239
Size: 393 1137 801 815 597 787 268 1524
Fragments arranged by size:
1524 1137 815 801 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3179 3179
Size: 6322
EcoRI G'AATT_C
Cuts at: 3631 4009 3631
Size: 378 5944
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2102 2102
Size: 6322
FspI TGC'GCA
Cuts at: 4828 4828
Size: 6322
HaeII r_GCGC'y
Cuts at: 1678 5469 1678
Size: 3791 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2535 2567 4963 4995 5037
Size: 32 135 32 963 32 2396 32 42
Cuts at: 5037 5069 1373
Size: 32 2626
Fragments arranged by size:
2626 2396 963 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3666 3912 669
Size: 988 281 1728 246 3079
Fragments arranged by size:
3079 1728 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6322
HpaI GTT'AAC
Cuts at: 3912 3912
Size: 6322
KpnI G_GTAC'C
Cuts at: 3647 3647
Size: 6322
MfeI C'AATT_G
Cuts at: 3921 3921
Size: 6322
MluI A'CGCG_T
Cuts at: 6198 6198
Size: 6322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2863 5436 5620 1305
Size: 1558 2573 184 2007
Fragments arranged by size:
2573 2007 1558 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4500
Fragments arranged by size:
4500 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2236 3309 4280 4639 4798 518
Size: 648 1070 1073 971 359 159 2042
Fragments arranged by size:
2042 1073 1070 971 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3127 3738 4415 5563 5808 1606
Size: 1521 611 677 1148 245 2120
Fragments arranged by size:
2120 1521 1148 677 611 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4831
Fragments arranged by size:
4831 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6322
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6322
NotI GC'GGCC_GC
Cuts at: 3769 3769
Size: 6322
NsiI A_TGCA'T
Cuts at: 2568 2568
Size: 6322
NspI r_CATG'y
Cuts at: 1132 1229 2475 2566 3680 5225 1132
Size: 97 1246 91 1114 1545 2229
Fragments arranged by size:
2229 1545 1246 1114 97 91
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6322
PciI A'CATG_T
Cuts at: 5221 5221
Size: 6322
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3383 1108
Size: 2275 4047
PfoI T'CCnGG_A
Cuts at: 2746 2746
Size: 6322
PmeI GTTT'AAAC
Cuts at: 3626 3626
Size: 6322
PmlI CAC'GTG
Cuts at: 3380 3380
Size: 6322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4391 4423 5904 5936 4391
Size: 32 1481 32 4777
Fragments arranged by size:
4777 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3179 3179
Size: 6322
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6322
PsiI TTA'TAA
Cuts at: 3892 3892
Size: 6322
PstI C_TGCA'G
Cuts at: 830 3472 3674 830
Size: 2642 202 3478
Fragments arranged by size:
3478 2642 202
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6322
PvuII CAG'CTG
Cuts at: 3127 3127
Size: 6322
SalI G'TCGA_C
Cuts at: 1936 3664 1936
Size: 1728 4594
SbfI CC_TGCA'GG
Cuts at: 3674 3674
Size: 6322
ScaI AGT'ACT
Cuts at: 3502 4570 6282 3502
Size: 1068 1712 3542
Fragments arranged by size:
3542 1712 1068
SfcI C'TryA_G
Cuts at: 826 1045 3110 3468 3670 4805 5486 5677 826
Size: 219 2065 358 202 1135 681 191 1471
Fragments arranged by size:
2065 1471 1135 681 358 219 202 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6322
SmaI CCC'GGG
Cuts at: 1872 3649 1872
Size: 1777 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2554 4426 5327 5589 820
Size: 197 926 611 1872 901 262 1553
Fragments arranged by size:
1872 1553 926 901 611 262 197
SnaBI TAC'GTA
Cuts at: 493 4113 493
Size: 3620 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6322
SphI G_CATG'C
Cuts at: 1132 2475 3680 1132
Size: 1343 1205 3774
Fragments arranged by size:
3774 1343 1205
SspI AAT'ATT
Cuts at: 5 52 4246 5
Size: 47 4194 2081
Fragments arranged by size:
4194 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6322
StyI C'CwwG_G
Cuts at: 513 1065 2004 2870 3761 513
Size: 552 939 866 891 3074
Fragments arranged by size:
3074 939 891 866 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2226 4349 4366 4519 4704 5149 325
Size: 1255 646 2123 17 153 185 445 1498
Fragments arranged by size:
2123 1498 1255 646 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3500 4568 6280
Size: 274 80 33 51 1443 1523 1068 1712
Cuts at: 6280 96
Size: 138
Fragments arranged by size:
1712 1523 1443 1068 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6282 6314 6282
Size: 32 6290
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6322
XbaI T'CTAG_A
Cuts at: 3055 3658 3055
Size: 603 5719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2231 2238 2231
Size: 7 6315
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6322
XmaI C'CCGG_G
Cuts at: 1870 3647 1870
Size: 1777 4545
XmnI GAAnn'nnTTC
Cuts at: 2337 3349 4451 2337
Size: 1012 1102 4208
Fragments arranged by size:
4208 1102 1012
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4130 276
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BplI Bpu10I BstZ17I EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI