Restriction map for
pFN21AB8703
(Circular) (Six-base) MAPSORT of: pFN21AB8703.seq Check: 7072 from: 1 to: 6427
pFN21AB8703 6427 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4237 278
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3770 1937
Size: 1833 4594
Acc65I G'GTAC_C
Cuts at: 3748 3748
Size: 6427
AclI AA'CG_TT
Cuts at: 1563 4554 4927 1563
Size: 2991 373 3063
Fragments arranged by size:
3063 2991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4490 5874 6142 1632
Size: 2858 1384 268 1917
Fragments arranged by size:
2858 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6230
AflIII A'CryG_T
Cuts at: 1203 3228 5326 6303 1203
Size: 2025 2098 977 1327
Fragments arranged by size:
2098 2025 1327 977
AgeI A'CCGG_T
Cuts at: 5258 5258
Size: 6427
AhdI GACnn_n'nnGTC
Cuts at: 5156 5156
Size: 6427
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2541 1184
Size: 1357 5070
AlwNI CAG_nnn'CTG
Cuts at: 2159 3260 3597 4170 5742 2159
Size: 1101 337 573 1572 2844
Fragments arranged by size:
2844 1572 1101 573 337
ApaI G_GGCC'C
Cuts at: 2661 2661
Size: 6427
ApaLI G'TGCA_C
Cuts at: 4484 5640 4484
Size: 1156 5271
ApoI r'AATT_y
Cuts at: 1493 2972 3736 3962 4114 6223 1493
Size: 1479 764 226 152 2109 1697
Fragments arranged by size:
2109 1697 1479 764 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4981 160
Size: 872 3949 1606
Fragments arranged by size:
3949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
AvaI C'yCGr_G
Cuts at: 1870 1943 2571 2716 3752 1870
Size: 73 628 145 1036 4545
Fragments arranged by size:
4545 1036 628 145 73
BamHI G'GATC_C
Cuts at: 3757 4124 3757
Size: 367 6060
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2460 2539 2788 3748 5203
Size: 325 217 627 673 79 249 960 1455
Cuts at: 5203 618
Size: 1842
Fragments arranged by size:
1842 1455 960 673 627 325 249 217
79
BanII G_rGCy'C
Cuts at: 1391 2661 2867 3746 1391
Size: 1270 206 879 4072
Fragments arranged by size:
4072 1270 879 206
BbeI G_GCGC'C
Cuts at: 2464 2464
Size: 6427
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2090 928
Size: 971 191 5265
Fragments arranged by size:
5265 971 191
BbvCI CC'TCA_GC
Cuts at: 2221 2619 3005 2221
Size: 398 386 5643
Fragments arranged by size:
5643 398 386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2349 2383 4618 4652 1903
Size: 34 412 34 2235 34 3678
Fragments arranged by size:
3678 2235 412 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4319 5529 4319
Size: 1210 5217
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6427
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2469 2656 5038
Size: 107 122 71 631 605 797 187 2382
Cuts at: 5038 136
Size: 1525
Fragments arranged by size:
2382 1525 797 631 605 187 122 107
71
BglII A'GATC_T
Cuts at: 3690 6422 3690
Size: 2732 3695
BlpI GC'TnA_GC
Cuts at: 3844 3844
Size: 6427
Bme1580I G_kGCm'C
Cuts at: 1790 2652 2661 4488 5644 1790
Size: 862 9 1827 1156 2573
Fragments arranged by size:
2573 1827 1156 862 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6427
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4158 5116 442
Size: 416 53 83 399 24 2741 958 1753
Fragments arranged by size:
2741 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6427
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2655 2655
Size: 6427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3053 3410 5087 1239
Size: 1814 357 1677 2579
Fragments arranged by size:
2579 1814 1677 357
Bpu10I CC'TnA_GC
Cuts at: 2043 2221 2619 3005 3480 2043
Size: 178 398 386 475 4990
Fragments arranged by size:
4990 475 398 386 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2062 2114 4552 5417 5715 2062
Size: 52 2438 865 298 2774
Fragments arranged by size:
2774 2438 865 298 52
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2643 3231 5090 882
Size: 617 1144 588 1859 2219
Fragments arranged by size:
2219 1859 1144 617 588
BsaAI yAC'GTr
Cuts at: 493 1204 3163 4218 493
Size: 711 1959 1055 2702
Fragments arranged by size:
2702 1959 1055 711
BsaBI GATnn'nnATC
Cuts at: 6421 6421
Size: 6427
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2188 2461 2531 4234
Size: 53 83 186 936 655 273 70 1703
Cuts at: 4234 4616 5251 275
Size: 382 635 1451
Fragments arranged by size:
1703 1451 936 655 635 382 273 186
83 70 53
BsaWI w'CCGG_w
Cuts at: 2403 4860 5258 5532 5679 6090 2403
Size: 2457 398 274 147 411 2740
Fragments arranged by size:
2740 2457 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3228 3258 1178
Size: 30 165 30 1825 30 4347
Fragments arranged by size:
4347 1825 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 2261 2264 2267 2270 2288 2691 2951
Size: 1063 12 3 3 3 18 403 260
Cuts at: 2951 1186
Size: 4662
Fragments arranged by size:
4662 1063 403 260 18 12 3 3
3
BseYI C'CCAG_C
Cuts at: 2068 2317 2328 2514 2593 3660 5630 6126 2068
Size: 249 11 186 79 1067 1970 496 2369
Fragments arranged by size:
2369 1970 1067 496 249 186 79 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2845 1163
Size: 573 1109 4745
Fragments arranged by size:
4745 1109 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3877 4638 5666 1540
Size: 275 186 1876 761 1028 2301
Fragments arranged by size:
2301 1876 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2611 4488 4573 5644 2611
Size: 1877 85 1071 3394
Fragments arranged by size:
3394 1877 1071 85
BsmI GAATG_Cn'
Cuts at: 3938 4031 3938
Size: 93 6334
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2611 2652 2661 2867 3746 4488 4573
Size: 399 821 41 9 206 879 742 85
Cuts at: 4573 5644 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 879 821 742 399 206 85
41 9
BspEI T'CCGG_A
Cuts at: 2403 6090 2403
Size: 3687 2740
BspHI T'CATG_A
Cuts at: 4316 6046 4316
Size: 1730 4697
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2375 2957 3782 844
Size: 1531 582 825 3489
Fragments arranged by size:
3489 1531 825 582
BsrBI CCG'CTC
Cuts at: 1394 1823 4314 1394
Size: 429 2491 3507
Fragments arranged by size:
3507 2491 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2824 3127 4922 5096 65
Size: 1183 1576 303 1795 174 1396
Fragments arranged by size:
1795 1576 1396 1183 303 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2472 2912 5071 5258 1700
Size: 110 662 440 2159 187 2869
Fragments arranged by size:
2869 2159 662 440 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6427
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6427
BssSI C'ACGA_G
Cuts at: 4487 5499 4487
Size: 1012 5415
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2823 3670 794
Size: 2029 847 3551
Fragments arranged by size:
3551 2029 847
BstBI TT'CG_AA
Cuts at: 5231 5231
Size: 6427
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6427
BstXI CCAn_nnnn'nTGG
Cuts at: 3346 3388 3346
Size: 42 6385
BstYI r'GATC_y
Cuts at: 1972 2155 3690 3757 4124 4509 4526 5978 6064
Size: 183 1535 67 367 385 17 1452 86
Cuts at: 6064 6076 6422 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1535 1452 385 367 346 183 86
67 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2664 3126 3171 513
Size: 552 1599 462 45 3769
Fragments arranged by size:
3769 1599 552 462 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5688
Fragments arranged by size:
5688 683 56
BtsI GCAGTG_nn'
Cuts at: 3709 3943 4737 4757 3709
Size: 234 794 20 5379
Fragments arranged by size:
5379 794 234 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6427
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2119 2154 529
Size: 35 1555 35 4802
Fragments arranged by size:
4802 1555 35 35
DraI TTT'AAA
Cuts at: 3731 3731
Size: 6427
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2695 5434 809
Size: 507 1379 2739 1802
Fragments arranged by size:
2739 1802 1379 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2914 3573 3874
Size: 54 1425 50 275 18 1084 659 301
Cuts at: 3874 4763 6392 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1084 889 659 301 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3874 1537
Size: 275 2062 4090
Fragments arranged by size:
4090 2062 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2226 2238 2265 2880 4357 1353
Size: 873 12 27 615 1477 3423
Fragments arranged by size:
3423 1477 873 615 27 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5014 5388 5534 387
Size: 1064 138 3425 374 146 1280
Fragments arranged by size:
3425 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3053 3410 4490 5087 5874 6142 1239
Size: 393 1421 357 1080 597 787 268 1524
Fragments arranged by size:
1524 1421 1080 787 597 393 357 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2163 2933 2163
Size: 770 5657
EcoO109I rG'GnC_Cy
Cuts at: 2031 2039 2482 2658 3076 3186 2031
Size: 8 443 176 418 110 5272
Fragments arranged by size:
5272 443 418 176 110 8
EcoRI G'AATT_C
Cuts at: 2972 3736 4114 2972
Size: 764 378 5285
Fragments arranged by size:
5285 764 378
FspI TGC'GCA
Cuts at: 4933 4933
Size: 6427
HaeII r_GCGC'y
Cuts at: 1678 2380 2464 5574 1678
Size: 702 84 3110 2531
Fragments arranged by size:
3110 2531 702 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 2699 2731 3228
Size: 32 135 32 575 32 520 32 497
Cuts at: 3228 3260 3420 3452 5068 5100 5142 5174 1373
Size: 32 160 32 1616 32 42 32 2626
Fragments arranged by size:
2626 1616 575 520 497 160 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3771 4017 669
Size: 988 281 1833 246 3079
Fragments arranged by size:
3079 1833 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2450 748
Size: 1702 4725
HpaI GTT'AAC
Cuts at: 4017 4017
Size: 6427
KasI G'GCGC_C
Cuts at: 2460 2460
Size: 6427
KpnI G_GTAC'C
Cuts at: 3752 3752
Size: 6427
MfeI C'AATT_G
Cuts at: 4026 4026
Size: 6427
MluI A'CGCG_T
Cuts at: 6303 6303
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5541 5725 1305
Size: 4236 184 2007
Fragments arranged by size:
4236 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3575 10
Size: 54 1425 343 1743 2862
Fragments arranged by size:
2862 1743 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4385 4744 4903 518
Size: 648 3219 359 159 2042
Fragments arranged by size:
3219 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2317 2332 3091 3109 3474 3843 4520 5668
Size: 711 15 759 18 365 369 677 1148
Cuts at: 5668 5913 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 759 711 677 369 365 245
18 15
NaeI GCC'GGC
Cuts at: 2474 2914 2474
Size: 440 5987
NarI GG'CG_CC
Cuts at: 2461 2461
Size: 6427
NcoI C'CATG_G
Cuts at: 513 1065 2664 3126 3171 513
Size: 552 1599 462 45 3769
Fragments arranged by size:
3769 1599 552 462 45
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6427
NgoMIV G'CCGG_C
Cuts at: 2472 2912 2472
Size: 440 5987
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6427
NotI GC'GGCC_GC
Cuts at: 3874 3874
Size: 6427
NspI r_CATG'y
Cuts at: 1132 1229 2945 3232 3673 3785 5330 1132
Size: 97 1716 287 441 112 1545 2229
Fragments arranged by size:
2229 1716 1545 441 287 112 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6427
PciI A'CATG_T
Cuts at: 3228 5326 3228
Size: 2098 4329
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2159 1108
Size: 1051 5376
PfoI T'CCnGG_A
Cuts at: 2050 3068 2050
Size: 1018 5409
PmeI GTTT'AAAC
Cuts at: 3731 3731
Size: 6427
PmlI CAC'GTG
Cuts at: 3163 3163
Size: 6427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 6009 6041 4496
Size: 32 1481 32 4882
Fragments arranged by size:
4882 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2031 3186 2031
Size: 1155 5272
PshAI GACnn'nnGTC
Cuts at: 1613 2131 1613
Size: 518 5909
PsiI TTA'TAA
Cuts at: 3153 3997 3153
Size: 844 5583
PspOMI G'GGCC_C
Cuts at: 2657 2657
Size: 6427
PstI C_TGCA'G
Cuts at: 830 3779 830
Size: 2949 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6427
PvuII CAG'CTG
Cuts at: 2317 3091 2317
Size: 774 5653
SalI G'TCGA_C
Cuts at: 1936 3769 1936
Size: 1833 4594
SbfI CC_TGCA'GG
Cuts at: 3779 3779
Size: 6427
ScaI AGT'ACT
Cuts at: 3620 4675 6387 3620
Size: 1055 1712 3660
Fragments arranged by size:
3660 1712 1055
SexAI A'CCwGG_T
Cuts at: 3216 3240 3216
Size: 24 6403
SfcI C'TryA_G
Cuts at: 826 1045 3500 3775 4910 5591 5782 826
Size: 219 2455 275 1135 681 191 1471
Fragments arranged by size:
2455 1471 1135 681 275 219 191
SfoI GGC'GCC
Cuts at: 2462 2462
Size: 6427
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6427
SmaI CCC'GGG
Cuts at: 1872 2573 3754 1872
Size: 701 1181 4545
Fragments arranged by size:
4545 1181 701
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2077 2129 4531 5432 5694 820
Size: 197 926 134 52 2402 901 262 1553
Fragments arranged by size:
2402 1553 926 901 262 197 134 52
SnaBI TAC'GTA
Cuts at: 493 4218 493
Size: 3725 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6427
SphI G_CATG'C
Cuts at: 1132 3785 1132
Size: 2653 3774
SspI AAT'ATT
Cuts at: 5 52 4351 5
Size: 47 4299 2081
Fragments arranged by size:
4299 2081 47
StuI AGG'CCT
Cuts at: 1517 2899 1517
Size: 1382 5045
StyI C'CwwG_G
Cuts at: 513 1065 2664 3126 3171 3309 3866 513
Size: 552 1599 462 45 138 557 3074
Fragments arranged by size:
3074 1599 557 552 462 138 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3540 3674 4454 4471 4624 4809 5254
Size: 1255 1960 134 780 17 153 185 445
Cuts at: 5254 325
Size: 1498
Fragments arranged by size:
1960 1498 1255 780 445 185 153 134
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2732 3618 4673
Size: 274 80 33 51 1443 755 886 1055
Cuts at: 4673 6385 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1055 886 755 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6387 6419 6387
Size: 32 6395
Tth111I GACn'n_nGTC
Cuts at: 1316 2029 3242 3254 1316
Size: 713 1213 12 4489
Fragments arranged by size:
4489 1213 713 12
XbaI T'CTAG_A
Cuts at: 3763 3763
Size: 6427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3076 3178 3076
Size: 102 6325
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6427
XmaI C'CCGG_G
Cuts at: 1870 2571 3752 1870
Size: 701 1181 4545
Fragments arranged by size:
4545 1181 701
XmnI GAAnn'nnTTC
Cuts at: 2976 4556 2976
Size: 1580 4847
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4235 276
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SrfI SwaI