Restriction map for
pFN21AB8702
(Circular) (Six-base) MAPSORT of: pFN21AB8702.seq Check: 2884 from: 1 to: 5818
pFN21AB8702 5818 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3016 3016
Size: 5818
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3628 278
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3161 1937
Size: 1224 4594
Acc65I G'GTAC_C
Cuts at: 3139 3139
Size: 5818
AclI AA'CG_TT
Cuts at: 1563 3945 4318 1563
Size: 2382 373 3063
Fragments arranged by size:
3063 2382 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 3881 5265 5533 1632
Size: 491 1758 1384 268 1917
Fragments arranged by size:
1917 1758 1384 491 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5621
AflIII A'CryG_T
Cuts at: 1203 4717 5694 1203
Size: 3514 977 1327
Fragments arranged by size:
3514 1327 977
AgeI A'CCGG_T
Cuts at: 4649 4649
Size: 5818
AhdI GACnn_n'nnGTC
Cuts at: 3113 4547 3113
Size: 1434 4384
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5818
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5818
AlwNI CAG_nnn'CTG
Cuts at: 2018 2326 2581 2632 2668 2887 3561 5133 2018
Size: 308 255 51 36 219 674 1572 2703
Fragments arranged by size:
2703 1572 674 308 255 219 51 36
ApaI G_GGCC'C
Cuts at: 2256 2706 2256
Size: 450 5368
ApaLI G'TGCA_C
Cuts at: 3875 5031 3875
Size: 1156 4662
ApoI r'AATT_y
Cuts at: 1493 3127 3353 3505 5614 1493
Size: 1634 226 152 2109 1697
Fragments arranged by size:
2109 1697 1634 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4372 160
Size: 872 3340 1606
Fragments arranged by size:
3340 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 2054 2255 2402 2441 2699 2705
Size: 73 104 7 201 147 39 258 6
Cuts at: 2705 2948 3143 1870
Size: 243 195 4545
Fragments arranged by size:
4545 258 243 201 195 147 104 73
39 7 6
BamHI G'GATC_C
Cuts at: 3148 3515 3148
Size: 367 5451
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2208 2381 2600 2689
Size: 325 217 627 252 169 173 219 89
Cuts at: 2689 2761 2783 3139 4594 618
Size: 72 22 356 1455 1842
Fragments arranged by size:
1842 1455 627 356 325 252 219 217
173 169 89 72 22
BanII G_rGCy'C
Cuts at: 1391 2256 2706 2958 3137 1391
Size: 865 450 252 179 4072
Fragments arranged by size:
4072 865 450 252 179
BbeI G_GCGC'C
Cuts at: 2043 2385 2604 2765 2787 2043
Size: 342 219 161 22 5074
Fragments arranged by size:
5074 342 219 161 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2605 928
Size: 971 706 4141
Fragments arranged by size:
4141 971 706
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4009 4043 1903
Size: 34 2072 34 3678
Fragments arranged by size:
3678 2072 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3710 4920 3710
Size: 1210 4608
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5818
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2031 2726
Size: 107 122 71 631 605 337 22 695
Cuts at: 2726 4429 136
Size: 1703 1525
Fragments arranged by size:
1703 1525 695 631 605 337 122 107
71 22
BglII A'GATC_T
Cuts at: 5813 5813
Size: 5818
BlpI GC'TnA_GC
Cuts at: 3054 3235 3054
Size: 181 5637
Bme1580I G_kGCm'C
Cuts at: 1790 2256 2706 3879 5035 1790
Size: 466 450 1173 1156 2573
Fragments arranged by size:
2573 1173 1156 466 450
BmgBI CAC'GTC
Cuts at: 1318 2797 1318
Size: 1479 4339
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3549 4507 442
Size: 416 53 83 399 24 2132 958 1753
Fragments arranged by size:
2132 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5818
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2584 2914 2584
Size: 330 5488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 2909 2954 4478 1239
Size: 1262 408 45 1524 2579
Fragments arranged by size:
2579 1524 1262 408 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3943 4808 5106 3943
Size: 865 298 4655
Fragments arranged by size:
4655 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4481 882
Size: 617 2982 2219
Fragments arranged by size:
2982 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3609 493
Size: 711 2405 2702
Fragments arranged by size:
2702 2405 711
BsaBI GATnn'nnATC
Cuts at: 5812 5812
Size: 5818
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 2382 2601 2714
Size: 53 83 186 936 507 342 219 113
Cuts at: 2714 2762 2784 3625 4007 4642 275
Size: 48 22 841 382 635 1451
Fragments arranged by size:
1451 936 841 635 507 382 342 219
186 113 83 53 48 22
BsaWI w'CCGG_w
Cuts at: 4251 4649 4923 5070 5481 4251
Size: 398 274 147 411 4588
Fragments arranged by size:
4588 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5593
Fragments arranged by size:
5593 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2828 2966 1186
Size: 1642 138 4038
Fragments arranged by size:
4038 1642 138
BseYI C'CCAG_C
Cuts at: 2161 2407 2511 2526 2694 5021 5517 2161
Size: 246 104 15 168 2327 496 2462
Fragments arranged by size:
2462 2327 496 246 168 104 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 2681 1163
Size: 573 645 300 4300
Fragments arranged by size:
4300 645 573 300
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2421 2481 2730 3268 4029 5057
Size: 275 186 420 60 249 538 761 1028
Cuts at: 5057 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 538 420 275 249 186
60
BsiHKAI G_wGCw'C
Cuts at: 2646 2877 2958 3879 3964 5035 2646
Size: 231 81 921 85 1071 3429
Fragments arranged by size:
3429 1071 921 231 85 81
BsmI GAATG_Cn'
Cuts at: 3329 3422 3329
Size: 93 5725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2256 2646 2706 2877 2958 3137 3879
Size: 399 466 390 60 171 81 179 742
Cuts at: 3879 3964 5035 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 466 399 390 179 171
85 81 60
BspEI T'CCGG_A
Cuts at: 5481 5481
Size: 5818
BspHI T'CATG_A
Cuts at: 3707 5437 3707
Size: 1730 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3016 3173 844
Size: 2172 157 3489
Fragments arranged by size:
3489 2172 157
BsrBI CCG'CTC
Cuts at: 1394 1823 3705 1394
Size: 429 1882 3507
Fragments arranged by size:
3507 1882 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4313 4487 65
Size: 1183 3065 174 1396
Fragments arranged by size:
3065 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2042 2082 2141 2416 2476 2489 2597
Size: 110 232 40 59 275 60 13 108
Cuts at: 2597 2686 2725 4462 4649 1700
Size: 89 39 1737 187 2869
Fragments arranged by size:
2869 1737 275 232 187 110 108 89
60 59 40 39 13
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5818
BssHII G'CGCG_C
Cuts at: 1925 2176 2225 2315 2503 2585 1925
Size: 251 49 90 188 82 5158
Fragments arranged by size:
5158 251 188 90 82 49
BssSI C'ACGA_G
Cuts at: 3878 4890 3878
Size: 1012 4806
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2018 2668 794
Size: 1224 650 3944
Fragments arranged by size:
3944 1224 650
BstBI TT'CG_AA
Cuts at: 4622 4622
Size: 5818
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5818
BstXI CCAn_nnnn'nTGG
Cuts at: 2377 2377
Size: 5818
BstYI r'GATC_y
Cuts at: 1972 3148 3515 3900 3917 5369 5455 5467 5813
Size: 1176 367 385 17 1452 86 12 346
Cuts at: 5813 1972
Size: 1977
Fragments arranged by size:
1977 1452 1176 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3103 3103
Size: 5818
BtgI C'CryG_G
Cuts at: 513 1065 2248 2385 2920 513
Size: 552 1183 137 535 3411
Fragments arranged by size:
3411 1183 552 535 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5079
Fragments arranged by size:
5079 683 56
BtsI GCAGTG_nn'
Cuts at: 2666 3334 4128 4148 2666
Size: 668 794 20 4336
Fragments arranged by size:
4336 794 668 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5818
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5783
DraI TTT'AAA
Cuts at: 3122 3122
Size: 5818
DraIII CAC_nnn'GTG
Cuts at: 2659 2659
Size: 5818
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2801 4825 809
Size: 507 1485 2024 1802
Fragments arranged by size:
2024 1802 1485 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2418 2448 2478
Size: 54 1425 50 275 18 588 30 30
Cuts at: 2478 2538 2727 2938 3038 3265 4154 5783 8
Size: 60 189 211 100 227 889 1629 43
Fragments arranged by size:
1629 1425 889 588 275 227 211 189
100 60 54 50 43 30 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2418 2478 2727 3265 1537
Size: 275 606 60 249 538 4090
Fragments arranged by size:
4090 606 538 275 249 60
EarI CTCTTCn'nnn_
Cuts at: 1353 2249 2297 3748 1353
Size: 896 48 1451 3423
Fragments arranged by size:
3423 1451 896 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2150 4405 4779 4925 387
Size: 1064 138 561 2255 374 146 1280
Fragments arranged by size:
2255 1280 1064 561 374 146 138
EcoICRI GAG'CTC
Cuts at: 2956 2956
Size: 5818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2501 2909 2954 3881 4478 5265
Size: 393 491 378 408 45 927 597 787
Cuts at: 5265 5533 1239
Size: 268 1524
Fragments arranged by size:
1524 927 787 597 491 408 393 378
268 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2276 2300 2276
Size: 24 5794
EcoO109I rG'GnC_Cy
Cuts at: 2051 2051
Size: 5818
EcoRI G'AATT_C
Cuts at: 3127 3505 3127
Size: 378 5440
FspI TGC'GCA
Cuts at: 4324 4324
Size: 5818
HaeII r_GCGC'y
Cuts at: 1678 2043 2243 2267 2326 2376 2385 2555 2604
Size: 365 200 24 59 50 9 170 49
Cuts at: 2604 2632 2765 2787 4965 1678
Size: 28 133 22 2178 2531
Fragments arranged by size:
2531 2178 365 200 170 133 59 50
49 28 24 22 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3099 3131 4459 4491 4533
Size: 32 135 32 1527 32 1328 32 42
Cuts at: 4533 4565 1373
Size: 32 2626
Fragments arranged by size:
2626 1527 1328 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3048 3162 3408 669
Size: 988 281 1110 114 246 3079
Fragments arranged by size:
3079 1110 988 281 246 114
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5818
HpaI GTT'AAC
Cuts at: 3408 3408
Size: 5818
KasI G'GCGC_C
Cuts at: 2039 2381 2600 2761 2783 2039
Size: 342 219 161 22 5074
Fragments arranged by size:
5074 342 219 161 22
KpnI G_GTAC'C
Cuts at: 3143 3143
Size: 5818
MfeI C'AATT_G
Cuts at: 3417 3417
Size: 5818
MluI A'CGCG_T
Cuts at: 5694 5694
Size: 5818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4932 5116 1305
Size: 3627 184 2007
Fragments arranged by size:
3627 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3040 10
Size: 54 1425 343 1208 2788
Fragments arranged by size:
2788 1425 1208 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2621 3776 4135 4294 518
Size: 648 1455 1155 359 159 2042
Fragments arranged by size:
2042 1455 1155 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2161 2215 2356 2387 2521 2530 2578
Size: 409 146 54 141 31 134 9 48
Cuts at: 2578 2808 2937 3234 3911 5059 5304 1606
Size: 230 129 297 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 409 297 245 230 146
141 134 129 54 48 31 9
NaeI GCC'GGC
Cuts at: 2044 2143 2418 2478 2688 2044
Size: 99 275 60 210 5174
Fragments arranged by size:
5174 275 210 99 60
NarI GG'CG_CC
Cuts at: 2040 2382 2601 2762 2784 2040
Size: 342 219 161 22 5074
Fragments arranged by size:
5074 342 219 161 22
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5266
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5818
NgoMIV G'CCGG_C
Cuts at: 2042 2141 2416 2476 2686 2042
Size: 99 275 60 210 5174
Fragments arranged by size:
5174 275 210 99 60
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5818
NotI GC'GGCC_GC
Cuts at: 3265 3265
Size: 5818
NspI r_CATG'y
Cuts at: 1132 1229 2626 3176 4721 1132
Size: 97 1397 550 1545 2229
Fragments arranged by size:
2229 1545 1397 550 97
PasI CC'CwG_GG
Cuts at: 1348 2377 2779 1348
Size: 1029 402 4387
Fragments arranged by size:
4387 1029 402
PciI A'CATG_T
Cuts at: 4717 4717
Size: 5818
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2326 2581 2887 2977 1108
Size: 1218 255 306 90 3949
Fragments arranged by size:
3949 1218 306 255 90
PmeI GTTT'AAAC
Cuts at: 3122 3122
Size: 5818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2738 2770 3887 3919 5400 5432 2738
Size: 32 1117 32 1481 32 3124
Fragments arranged by size:
3124 1481 1117 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2051 2051
Size: 5818
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5818
PsiI TTA'TAA
Cuts at: 3388 3388
Size: 5818
PspOMI G'GGCC_C
Cuts at: 2252 2702 2252
Size: 450 5368
PspXI vC'TCGA_Gb
Cuts at: 2402 2441 2402
Size: 39 5779
PstI C_TGCA'G
Cuts at: 830 2023 2361 2550 3027 3170 830
Size: 1193 338 189 477 143 3478
Fragments arranged by size:
3478 1193 477 338 189 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5818
PvuII CAG'CTG
Cuts at: 2161 2215 2521 2530 2578 2161
Size: 54 306 9 48 5401
Fragments arranged by size:
5401 306 54 48 9
SacI G_AGCT'C
Cuts at: 2958 2958
Size: 5818
SacII CC_GC'GG
Cuts at: 2388 2388
Size: 5818
SalI G'TCGA_C
Cuts at: 1936 3160 1936
Size: 1224 4594
SanDI GG'GwC_CC
Cuts at: 2051 2051
Size: 5818
SapI GCTCTTCn'nnn_
Cuts at: 2249 2249
Size: 5818
SbfI CC_TGCA'GG
Cuts at: 3170 3170
Size: 5818
ScaI AGT'ACT
Cuts at: 2897 4066 5778 2897
Size: 1169 1712 2937
Fragments arranged by size:
2937 1712 1169
SfcI C'TryA_G
Cuts at: 826 1045 2019 2357 2546 3023 3166 4301 4982
Size: 219 974 338 189 477 143 1135 681
Cuts at: 4982 5173 826
Size: 191 1471
Fragments arranged by size:
1471 1135 974 681 477 338 219 191
189 143
SfoI GGC'GCC
Cuts at: 2041 2383 2602 2763 2785 2041
Size: 342 219 161 22 5074
Fragments arranged by size:
5074 342 219 161 22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
SgrAI Cr'CCGG_yG
Cuts at: 1700 2597 1700
Size: 897 4921
SmaI CCC'GGG
Cuts at: 1872 2049 2257 2701 2707 3145 1872
Size: 177 208 444 6 438 4545
Fragments arranged by size:
4545 444 438 208 177 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2402 2441 3922 4823 5085 820
Size: 197 926 459 39 1481 901 262 1553
Fragments arranged by size:
1553 1481 926 901 459 262 197 39
SnaBI TAC'GTA
Cuts at: 493 3609 493
Size: 3116 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5818
SphI G_CATG'C
Cuts at: 1132 2626 3176 1132
Size: 1494 550 3774
Fragments arranged by size:
3774 1494 550
SrfI GCCC'GGGC
Cuts at: 2701 2701
Size: 5818
SspI AAT'ATT
Cuts at: 5 52 3742 5
Size: 47 3690 2081
Fragments arranged by size:
3690 2081 47
StuI AGG'CCT
Cuts at: 1517 2025 1517
Size: 508 5310
StyI C'CwwG_G
Cuts at: 513 1065 3257 513
Size: 552 2192 3074
Fragments arranged by size:
3074 2192 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3845 3862 4015 4200 4645 325
Size: 1255 2265 17 153 185 445 1498
Fragments arranged by size:
2265 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2895 4064 5776
Size: 274 80 33 51 1443 918 1169 1712
Cuts at: 5776 96
Size: 138
Fragments arranged by size:
1712 1443 1169 918 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5778 5810 5778
Size: 32 5786
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5818
XbaI T'CTAG_A
Cuts at: 3154 3154
Size: 5818
XhoI C'TCGA_G
Cuts at: 1943 2402 2441 1943
Size: 459 39 5320
Fragments arranged by size:
5320 459 39
XmaI C'CCGG_G
Cuts at: 1870 2047 2255 2699 2705 3143 1870
Size: 177 208 444 6 438 4545
Fragments arranged by size:
4545 444 438 208 177 6
XmnI GAAnn'nnTTC
Cuts at: 2111 3947 2111
Size: 1836 3982
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3626 276
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PsrI RsrII SexAI SfiI
SwaI XcmI