Restriction map for
pFN21AB8699
(Circular) (Six-base) MAPSORT of: pFN21AB8699.seq Check: 4967 from: 1 to: 6328
pFN21AB8699 6328 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4138 278
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3671 1937
Size: 1734 4594
Acc65I G'GTAC_C
Cuts at: 2111 3649 2111
Size: 1538 4790
AclI AA'CG_TT
Cuts at: 1563 3347 4455 4828 1563
Size: 1784 1108 373 3063
Fragments arranged by size:
3063 1784 1108 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2149 2213 4391 5775 6043 1632
Size: 517 64 2178 1384 268 1917
Fragments arranged by size:
2178 1917 1384 517 268 64
AflII C'TTAA_G
Cuts at: 820 1017 2603 820
Size: 197 1586 4545
Fragments arranged by size:
4545 1586 197
AflIII A'CryG_T
Cuts at: 1203 3017 5227 6204 1203
Size: 1814 2210 977 1327
Fragments arranged by size:
2210 1814 1327 977
AgeI A'CCGG_T
Cuts at: 5159 5159
Size: 6328
AhdI GACnn_n'nnGTC
Cuts at: 5057 5057
Size: 6328
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6328
AlwNI CAG_nnn'CTG
Cuts at: 3530 4071 5643 3530
Size: 541 1572 4215
Fragments arranged by size:
4215 1572 541
ApaLI G'TGCA_C
Cuts at: 2351 4385 5541 2351
Size: 2034 1156 3138
Fragments arranged by size:
3138 2034 1156
ApoI r'AATT_y
Cuts at: 1493 2730 3211 3377 3455 3560 3637 3863 4015
Size: 1237 481 166 78 105 77 226 152
Cuts at: 4015 6124 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1237 481 226 166 152 105
78 77
AseI AT'TA_AT
Cuts at: 160 1032 4882 160
Size: 872 3850 1606
Fragments arranged by size:
3850 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 3653 1870
Size: 73 135 1575 4545
Fragments arranged by size:
4545 1575 135 73
BamHI G'GATC_C
Cuts at: 3658 4025 3658
Size: 367 5961
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 2225 2294 2713 2860
Size: 325 217 627 324 114 69 419 147
Cuts at: 2860 3649 5104 618
Size: 789 1455 1842
Fragments arranged by size:
1842 1455 789 627 419 325 324 217
147 114 69
BanII G_rGCy'C
Cuts at: 1391 2079 2543 3647 1391
Size: 688 464 1104 4072
Fragments arranged by size:
4072 1104 688 464
BbeI G_GCGC'C
Cuts at: 2229 2864 2229
Size: 635 5693
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2356 3235 3609 928
Size: 971 457 879 374 3647
Fragments arranged by size:
3647 971 879 457 374
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4519 4553 1903
Size: 34 2582 34 3678
Fragments arranged by size:
3678 2582 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4220 5430 4220
Size: 1210 5118
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6328
BfrBI ATG'CAT
Cuts at: 2747 2747
Size: 6328
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2288 4939 136
Size: 107 122 71 631 605 616 2651 1525
Fragments arranged by size:
2651 1525 631 616 605 122 107 71
BglII A'GATC_T
Cuts at: 6323 6323
Size: 6328
BlpI GC'TnA_GC
Cuts at: 3745 3745
Size: 6328
Bme1580I G_kGCm'C
Cuts at: 1790 2355 2485 3025 4389 5545 1790
Size: 565 130 540 1364 1156 2573
Fragments arranged by size:
2573 1364 1156 565 540 130
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6328
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2266 2704 4059
Size: 416 53 83 399 24 849 438 1355
Cuts at: 4059 5017 442
Size: 958 1753
Fragments arranged by size:
1753 1355 958 849 438 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6328
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 3152
Size: 6328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2493 3462 4988 1239
Size: 854 400 969 1526 2579
Fragments arranged by size:
2579 1526 969 854 400
Bpu10I CC'TnA_GC
Cuts at: 2529 2529
Size: 6328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4453 5318 5616 4453
Size: 865 298 5165
Fragments arranged by size:
5165 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4991 882
Size: 617 3492 2219
Fragments arranged by size:
3492 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4119 493
Size: 711 2915 2702
Fragments arranged by size:
2915 2702 711
BsaBI GATnn'nnATC
Cuts at: 6322 6322
Size: 6328
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2226 2861 4135 4517
Size: 53 83 186 936 693 635 1274 382
Cuts at: 4517 5152 275
Size: 635 1451
Fragments arranged by size:
1451 1274 936 693 635 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4761 5159 5433 5580 5991 4761
Size: 398 274 147 411 5098
Fragments arranged by size:
5098 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2237 2267 1178
Size: 30 165 30 834 30 5239
Fragments arranged by size:
5239 834 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2200 2380 2395 3237 3240 3294 1186
Size: 1014 180 15 842 3 54 4220
Fragments arranged by size:
4220 1014 842 180 54 15 3
BseYI C'CCAG_C
Cuts at: 2048 2709 5531 6027 2048
Size: 661 2822 496 2349
Fragments arranged by size:
2822 2349 661 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2228 1163
Size: 573 492 5263
Fragments arranged by size:
5263 573 492
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3778 4539 5567 1540
Size: 275 186 1777 761 1028 2301
Fragments arranged by size:
2301 1777 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2355 4389 4474 5545 2355
Size: 2034 85 1071 3138
Fragments arranged by size:
3138 2034 1071 85
BsmI GAATG_Cn'
Cuts at: 3839 3932 3839
Size: 93 6235
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2355 2485 2543 3025 3647 4389
Size: 399 289 276 130 58 482 622 742
Cuts at: 4389 4474 5545 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 622 482 399 289 276
130 85 58
BspEI T'CCGG_A
Cuts at: 5991 5991
Size: 6328
BspHI T'CATG_A
Cuts at: 2920 4217 5947 2920
Size: 1297 1730 3301
Fragments arranged by size:
3301 1730 1297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2076 3683 844
Size: 1232 1607 3489
Fragments arranged by size:
3489 1607 1232
BsrBI CCG'CTC
Cuts at: 1394 1823 2025 4215 1394
Size: 429 202 2190 3507
Fragments arranged by size:
3507 2190 429 202
BsrDI GCAATG_nn'
Cuts at: 65 1248 4823 4997 65
Size: 1183 3575 174 1396
Fragments arranged by size:
3575 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2181 2291 4972 5159 1700
Size: 110 371 110 2681 187 2869
Fragments arranged by size:
2869 2681 371 187 110 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6328
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6328
BssSI C'ACGA_G
Cuts at: 2354 4388 5400 2354
Size: 2034 1012 3282
Fragments arranged by size:
3282 2034 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6328
BstBI TT'CG_AA
Cuts at: 3157 3226 5132 3157
Size: 69 1906 4353
Fragments arranged by size:
4353 1906 69
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6328
BstYI r'GATC_y
Cuts at: 1972 2019 3658 4025 4410 4427 5879 5965 5977
Size: 47 1639 367 385 17 1452 86 12
Cuts at: 5977 6323 1972
Size: 346 1977
Fragments arranged by size:
1977 1639 1452 385 367 346 86 47
17 12
Bsu36I CC'TnA_GG
Cuts at: 3385 3385
Size: 6328
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5776
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5589
Fragments arranged by size:
5589 683 56
BtsI GCAGTG_nn'
Cuts at: 2027 2977 3844 4638 4658 2027
Size: 950 867 794 20 3697
Fragments arranged by size:
3697 950 867 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6328
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6293
DraI TTT'AAA
Cuts at: 3352 3632 3352
Size: 280 6048
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5335 809
Size: 507 4019 1802
Fragments arranged by size:
4019 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2289 3775 4664
Size: 54 1425 50 275 18 459 1486 889
Cuts at: 4664 6293 8
Size: 1629 43
Fragments arranged by size:
1629 1486 1425 889 459 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3775 1537
Size: 275 1963 4090
Fragments arranged by size:
4090 1963 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2943 3055 3121 3138 4258 1353
Size: 1590 112 66 17 1120 3423
Fragments arranged by size:
3423 1590 1120 112 66 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2564 4915 5289 5435 387
Size: 1064 138 975 2351 374 146 1280
Fragments arranged by size:
2351 1280 1064 975 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2149 2213 2493 3462 4391 4988
Size: 393 461 56 64 280 969 929 597
Cuts at: 4988 5775 6043 1239
Size: 787 268 1524
Fragments arranged by size:
1524 969 929 787 597 461 393 280
268 64 56
EcoO109I rG'GnC_Cy
Cuts at: 2065 2065
Size: 6328
EcoRI G'AATT_C
Cuts at: 2730 3637 4015 2730
Size: 907 378 5043
Fragments arranged by size:
5043 907 378
FspI TGC'GCA
Cuts at: 4834 4834
Size: 6328
HaeII r_GCGC'y
Cuts at: 1678 2229 2864 5475 1678
Size: 551 635 2611 2531
Fragments arranged by size:
2611 2531 635 551
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2572 2604 2966 2998 4969
Size: 32 135 32 1000 32 362 32 1971
Cuts at: 4969 5001 5043 5075 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1971 1000 362 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3672 3918 669
Size: 988 281 1734 246 3079
Fragments arranged by size:
3079 1734 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2148 2600 2836 3446 3470 748
Size: 1400 452 236 610 24 3606
Fragments arranged by size:
3606 1400 610 452 236 24
HpaI GTT'AAC
Cuts at: 3918 3918
Size: 6328
KasI G'GCGC_C
Cuts at: 2225 2860 2225
Size: 635 5693
KpnI G_GTAC'C
Cuts at: 2115 3653 2115
Size: 1538 4790
MfeI C'AATT_G
Cuts at: 3927 3927
Size: 6328
MluI A'CGCG_T
Cuts at: 3017 6204 3017
Size: 3187 3141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5442 5626 1305
Size: 4137 184 2007
Fragments arranged by size:
4137 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4506
Fragments arranged by size:
4506 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3138 4286 4645 4804 518
Size: 648 1972 1148 359 159 2042
Fragments arranged by size:
2042 1972 1148 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2191 2242 3744 4421 5569 5814 1606
Size: 585 51 1502 677 1148 245 2120
Fragments arranged by size:
2120 1502 1148 677 585 245 51
NaeI GCC'GGC
Cuts at: 2293 2293
Size: 6328
NarI GG'CG_CC
Cuts at: 2226 2861 2226
Size: 635 5693
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5776
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6328
NgoMIV G'CCGG_C
Cuts at: 2291 2291
Size: 6328
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6328
NotI GC'GGCC_GC
Cuts at: 3775 3775
Size: 6328
NsiI A_TGCA'T
Cuts at: 2749 2749
Size: 6328
NspI r_CATG'y
Cuts at: 1132 1229 2751 3686 5231 1132
Size: 97 1522 935 1545 2229
Fragments arranged by size:
2229 1545 1522 935 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6328
PciI A'CATG_T
Cuts at: 5227 5227
Size: 6328
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6328
PmeI GTTT'AAAC
Cuts at: 3632 3632
Size: 6328
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3444 3476 4397 4429 5910 5942 3444
Size: 32 921 32 1481 32 3830
Fragments arranged by size:
3830 1481 921 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2065 2065
Size: 6328
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6328
PsiI TTA'TAA
Cuts at: 3898 3898
Size: 6328
PstI C_TGCA'G
Cuts at: 830 3680 830
Size: 2850 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6328
PvuII CAG'CTG
Cuts at: 2191 2191
Size: 6328
SalI G'TCGA_C
Cuts at: 1936 3670 1936
Size: 1734 4594
SbfI CC_TGCA'GG
Cuts at: 3680 3680
Size: 6328
ScaI AGT'ACT
Cuts at: 4576 6288 4576
Size: 1712 4616
SexAI A'CCwGG_T
Cuts at: 3090 3090
Size: 6328
SfcI C'TryA_G
Cuts at: 826 1045 2186 3676 4811 5492 5683 826
Size: 219 1141 1490 1135 681 191 1471
Fragments arranged by size:
1490 1471 1141 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2288 2288
Size: 6328
SfoI GGC'GCC
Cuts at: 2227 2862 2227
Size: 635 5693
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6328
SmaI CCC'GGG
Cuts at: 1872 2080 3655 1872
Size: 208 1575 4545
Fragments arranged by size:
4545 1575 208
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2603 4432 5333 5595 820
Size: 197 926 660 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 660 262 197
SnaBI TAC'GTA
Cuts at: 493 4119 493
Size: 3626 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6328
SphI G_CATG'C
Cuts at: 1132 3686 1132
Size: 2554 3774
SspI AAT'ATT
Cuts at: 5 52 4252 5
Size: 47 4200 2081
Fragments arranged by size:
4200 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6328
StyI C'CwwG_G
Cuts at: 513 1065 3767 513
Size: 552 2702 3074
Fragments arranged by size:
3074 2702 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2701 4355 4372 4525 4710 5155 325
Size: 1255 1121 1654 17 153 185 445 1498
Fragments arranged by size:
1654 1498 1255 1121 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4574 6286 96
Size: 274 80 33 51 1443 2597 1712 138
Fragments arranged by size:
2597 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2237 2269 6288 6320 2237
Size: 32 4019 32 2245
Fragments arranged by size:
4019 2245 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6328
XbaI T'CTAG_A
Cuts at: 2683 3664 2683
Size: 981 5347
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6328
XmaI C'CCGG_G
Cuts at: 1870 2078 3653 1870
Size: 208 1575 4545
Fragments arranged by size:
4545 1575 208
XmnI GAAnn'nnTTC
Cuts at: 3459 4457 3459
Size: 998 5330
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4136 276
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SrfI
SwaI XcmI