Restriction map for
pFN21AB8692
(Circular) (Six-base) MAPSORT of: pFN21AB8692.seq Check: 5536 from: 1 to: 6142
pFN21AB8692 6142 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3952 278
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3485 1937
Size: 1548 4594
Acc65I G'GTAC_C
Cuts at: 3463 3463
Size: 6142
AclI AA'CG_TT
Cuts at: 1563 3382 4269 4642 1563
Size: 1819 887 373 3063
Fragments arranged by size:
3063 1819 887 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3152 3216 4205 5589 5857 1632
Size: 1520 64 989 1384 268 1917
Fragments arranged by size:
1917 1520 1384 989 268 64
AfeI AGC'GCT
Cuts at: 2529 2529
Size: 6142
AflII C'TTAA_G
Cuts at: 820 1017 2987 820
Size: 197 1970 3975
Fragments arranged by size:
3975 1970 197
AflIII A'CryG_T
Cuts at: 1203 5041 6018 1203
Size: 3838 977 1327
Fragments arranged by size:
3838 1327 977
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 2891 4871 2891
Size: 1980 4162
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6142
AlwNI CAG_nnn'CTG
Cuts at: 2956 3885 5457 2956
Size: 929 1572 3641
Fragments arranged by size:
3641 1572 929
ApaLI G'TGCA_C
Cuts at: 4199 5355 4199
Size: 1156 4986
ApoI r'AATT_y
Cuts at: 1493 2920 3451 3677 3829 5938 1493
Size: 1427 531 226 152 2109 1697
Fragments arranged by size:
2109 1697 1427 531 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 2172 2439 3467 1870
Size: 73 229 267 1028 4545
Fragments arranged by size:
4545 1028 267 229 73
AvrII C'CTAG_G
Cuts at: 3272 3272
Size: 6142
BamHI G'GATC_C
Cuts at: 2374 3472 3839 2374
Size: 1098 367 4677
Fragments arranged by size:
4677 1098 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2514 3463 4918 618
Size: 325 217 627 727 949 1455 1842
Fragments arranged by size:
1842 1455 949 727 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2345 3461 1391
Size: 954 1116 4072
Fragments arranged by size:
4072 1116 954
BbeI G_GCGC'C
Cuts at: 2518 2518
Size: 6142
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2703 3159 928
Size: 971 804 456 3911
Fragments arranged by size:
3911 971 804 456
BbvCI CC'TCA_GC
Cuts at: 2361 2361
Size: 6142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4333 4367 1903
Size: 34 2396 34 3678
Fragments arranged by size:
3678 2396 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4034 5244 4034
Size: 1210 4932
BclI T'GATC_A
Cuts at: 1548 2925 1548
Size: 1377 4765
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4753 136
Size: 107 122 71 631 605 3081 1525
Fragments arranged by size:
3081 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3091 6137 3091
Size: 3046 3096
BlpI GC'TnA_GC
Cuts at: 3559 3559
Size: 6142
Bme1580I G_kGCm'C
Cuts at: 1790 4203 5359 1790
Size: 2413 1156 2573
Fragments arranged by size:
2573 2413 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6142
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2373 3873 4831
Size: 416 53 83 399 24 956 1500 958
Cuts at: 4831 442
Size: 1753
Fragments arranged by size:
1753 1500 958 956 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2255 1056
Size: 1199 4943
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2756 4802 1239
Size: 1517 2046 2579
Fragments arranged by size:
2579 2046 1517
Bpu10I CC'TnA_GC
Cuts at: 2182 2187 2361 2182
Size: 5 174 5963
Fragments arranged by size:
5963 174 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2818 4267 5132 5430 2818
Size: 1449 865 298 3530
Fragments arranged by size:
3530 1449 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4805 882
Size: 617 3306 2219
Fragments arranged by size:
3306 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3933 493
Size: 711 2729 2702
Fragments arranged by size:
2729 2702 711
BsaBI GATnn'nnATC
Cuts at: 3294 6136 3294
Size: 2842 3300
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2515 3949 4331 4966
Size: 53 83 186 936 982 1434 382 635
Cuts at: 4966 275
Size: 1451
Fragments arranged by size:
1451 1434 982 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4575 4973 5247 5394 5805 4575
Size: 398 274 147 411 4912
Fragments arranged by size:
4912 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2729 2759 1178
Size: 30 165 30 1326 30 4561
Fragments arranged by size:
4561 1326 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2234 2246 2249 2252 2255 2258 2318 2321
Size: 1048 12 3 3 3 3 60 3
Cuts at: 2321 2324 2327 2330 2483 2486 2498 2501 2504
Size: 3 3 3 153 3 12 3 3
Cuts at: 2504 2507 1186
Size: 3 4821
Fragments arranged by size:
4821 1048 153 60 12 12 3 3
3 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2875 5345 5841 2875
Size: 2470 496 3176
Fragments arranged by size:
3176 2470 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3432 1163
Size: 573 1696 3873
Fragments arranged by size:
3873 1696 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3592 4353 5381 1540
Size: 275 186 1591 761 1028 2301
Fragments arranged by size:
2301 1591 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2345 4203 4288 5359 2345
Size: 1858 85 1071 3128
Fragments arranged by size:
3128 1858 1071 85
BsmI GAATG_Cn'
Cuts at: 2299 2326 3405 3653 3746 2299
Size: 27 1079 248 93 4695
Fragments arranged by size:
4695 1079 248 93 27
BsmBI CGTCTCn'nnnn_
Cuts at: 2208 2208
Size: 6142
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2345 3461 4203 4288 5359 1391
Size: 399 555 1116 742 85 1071 2174
Fragments arranged by size:
2174 1116 1071 742 555 399 85
BspEI T'CCGG_A
Cuts at: 5805 5805
Size: 6142
BspHI T'CATG_A
Cuts at: 2158 4031 5761 2158
Size: 1873 1730 2539
Fragments arranged by size:
2539 1873 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2054 3497 844
Size: 1210 1443 3489
Fragments arranged by size:
3489 1443 1210
BsrBI CCG'CTC
Cuts at: 1394 1823 4029 1394
Size: 429 2206 3507
Fragments arranged by size:
3507 2206 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4637 4811 65
Size: 1183 3389 174 1396
Fragments arranged by size:
3389 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2445 4786 4973 1700
Size: 110 635 2341 187 2869
Fragments arranged by size:
2869 2341 635 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6142
BssSI C'ACGA_G
Cuts at: 4202 5214 4202
Size: 1012 5130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6142
BstBI TT'CG_AA
Cuts at: 4946 4946
Size: 6142
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6142
BstYI r'GATC_y
Cuts at: 1972 2015 2374 3091 3472 3839 4224 4241 5693
Size: 43 359 717 381 367 385 17 1452
Cuts at: 5693 5779 5791 6137 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 717 385 381 367 359 346
86 43 17 12
Bsu36I CC'TnA_GG
Cuts at: 2586 2586
Size: 6142
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4651
Fragments arranged by size:
4651 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5403
Fragments arranged by size:
5403 683 56
BtsI GCAGTG_nn'
Cuts at: 3658 4452 4472 3658
Size: 794 20 5328
Fragments arranged by size:
5328 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6107
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2705 5149 809
Size: 507 1389 2444 1802
Fragments arranged by size:
2444 1802 1389 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3589 4478 6107
Size: 54 1425 50 275 18 1759 889 1629
Cuts at: 6107 8
Size: 43
Fragments arranged by size:
1759 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 1537
Size: 275 1777 4090
Fragments arranged by size:
4090 1777 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3322 3340 3369 4072 1353
Size: 1969 18 29 703 3423
Fragments arranged by size:
3423 1969 703 29 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4729 5103 5249 387
Size: 1064 138 3140 374 146 1280
Fragments arranged by size:
3140 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2343 2343
Size: 6142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2756 3152 3216 4205 4802 5589 5857
Size: 393 1124 396 64 989 597 787 268
Cuts at: 5857 1239
Size: 1524
Fragments arranged by size:
1524 1124 989 787 597 396 393 268
64
EcoO109I rG'GnC_Cy
Cuts at: 2066 2066
Size: 6142
EcoRI G'AATT_C
Cuts at: 3451 3829 3451
Size: 378 5764
FspI TGC'GCA
Cuts at: 4648 4648
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 2518 2531 5289 1678
Size: 840 13 2758 2531
Fragments arranged by size:
2758 2531 840 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2153 2185 2877 2909 4783
Size: 32 135 32 581 32 692 32 1874
Cuts at: 4783 4815 4857 4889 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1874 692 581 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2456 2715 2982 3486 3732 669
Size: 988 281 518 259 267 504 246 3079
Fragments arranged by size:
3079 988 518 504 281 267 259 246
HindIII A'AGCT_T
Cuts at: 748 3434 748
Size: 2686 3456
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KasI G'GCGC_C
Cuts at: 2514 2514
Size: 6142
KpnI G_GTAC'C
Cuts at: 3467 3467
Size: 6142
MfeI C'AATT_G
Cuts at: 3741 3741
Size: 6142
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5256 5440 1305
Size: 3951 184 2007
Fragments arranged by size:
3951 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4320
Fragments arranged by size:
4320 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4100 4459 4618 518
Size: 648 2934 359 159 2042
Fragments arranged by size:
2934 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 2512 3558 4235 5383 5628 1606
Size: 442 464 1046 677 1148 245 2120
Fragments arranged by size:
2120 1148 1046 677 464 442 245
NaeI GCC'GGC
Cuts at: 2447 2447
Size: 6142
NarI GG'CG_CC
Cuts at: 2515 2515
Size: 6142
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4651
Fragments arranged by size:
4651 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6142
NgoMIV G'CCGG_C
Cuts at: 2445 2445
Size: 6142
NheI G'CTAG_C
Cuts at: 1052 2251 1052
Size: 1199 4943
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 3500 5045 1132
Size: 97 2271 1545 2229
Fragments arranged by size:
2271 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6142
PciI A'CATG_T
Cuts at: 5041 5041
Size: 6142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2882 1108
Size: 1774 4368
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3339 3371 4211 4243 5724 5756 3339
Size: 32 840 32 1481 32 3725
Fragments arranged by size:
3725 1481 840 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2066 2066
Size: 6142
PshAI GACnn'nnGTC
Cuts at: 1613 2609 1613
Size: 996 5146
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PstI C_TGCA'G
Cuts at: 830 3494 830
Size: 2664 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6142
PvuII CAG'CTG
Cuts at: 2048 2048
Size: 6142
SacI G_AGCT'C
Cuts at: 2345 2345
Size: 6142
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SapI GCTCTTCn'nnn_
Cuts at: 3322 3369 3322
Size: 47 6095
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 3137 4390 6102 3137
Size: 1253 1712 3177
Fragments arranged by size:
3177 1712 1253
SfcI C'TryA_G
Cuts at: 826 1045 3490 4625 5306 5497 826
Size: 219 2445 1135 681 191 1471
Fragments arranged by size:
2445 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2516 2516
Size: 6142
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 2174 3469 1872
Size: 302 1295 4545
Fragments arranged by size:
4545 1295 302
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2797 2987 4246 5147 5409 820
Size: 197 926 854 190 1259 901 262 1553
Fragments arranged by size:
1553 1259 926 901 854 262 197 190
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 3500 1132
Size: 2368 3774
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 2366 2567 1517
Size: 849 201 5092
Fragments arranged by size:
5092 849 201
StyI C'CwwG_G
Cuts at: 513 1065 2004 2058 3272 3581 513
Size: 552 939 54 1214 309 3074
Fragments arranged by size:
3074 1214 939 552 309 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4169 4186 4339 4524 4969 325
Size: 1255 2589 17 153 185 445 1498
Fragments arranged by size:
2589 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2203 2808 3135
Size: 274 80 33 51 1443 226 605 327
Cuts at: 3135 4388 6100 96
Size: 1253 1712 138
Fragments arranged by size:
1712 1443 1253 605 327 274 226 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6102 6134 6102
Size: 32 6110
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6142
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6142
XmaI C'CCGG_G
Cuts at: 1870 2172 3467 1870
Size: 302 1295 4545
Fragments arranged by size:
4545 1295 302
XmnI GAAnn'nnTTC
Cuts at: 3015 4271 3015
Size: 1256 4886
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3950 276
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BfrBI BplI BsiWI
BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI