Restriction map for
pFN21AB8691
(Circular) (Six-base) MAPSORT of: pFN21AB8691.seq Check: 6701 from: 1 to: 6784
pFN21AB8691 6784 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4594 278
Size: 53 83 186 936 3058 2468
Fragments arranged by size:
3058 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4127 1937
Size: 2190 4594
Acc65I G'GTAC_C
Cuts at: 4105 4105
Size: 6784
AclI AA'CG_TT
Cuts at: 1563 4911 5284 1563
Size: 3348 373 3063
Fragments arranged by size:
3348 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3579 4019 4847 6231 6499 1632
Size: 1947 440 828 1384 268 1917
Fragments arranged by size:
1947 1917 1384 828 440 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6587
AflIII A'CryG_T
Cuts at: 1203 5683 6660 1203
Size: 4480 977 1327
Fragments arranged by size:
4480 1327 977
AgeI A'CCGG_T
Cuts at: 5615 5615
Size: 6784
AhdI GACnn_n'nnGTC
Cuts at: 5513 5513
Size: 6784
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6784
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6784
AlwNI CAG_nnn'CTG
Cuts at: 2176 2659 3131 4527 6099 2176
Size: 483 472 1396 1572 2861
Fragments arranged by size:
2861 1572 1396 483 472
ApaI G_GGCC'C
Cuts at: 2207 2440 2207
Size: 233 6551
ApaLI G'TGCA_C
Cuts at: 4841 5997 4841
Size: 1156 5628
ApoI r'AATT_y
Cuts at: 1493 2829 4093 4319 4471 6580 1493
Size: 1336 1264 226 152 2109 1697
Fragments arranged by size:
2109 1697 1336 1264 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5338 160
Size: 872 4306 1606
Fragments arranged by size:
4306 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6784
AvaI C'yCGr_G
Cuts at: 1870 1943 2140 2240 2374 4109 1870
Size: 73 197 100 134 1735 4545
Fragments arranged by size:
4545 1735 197 134 100 73
BamHI G'GATC_C
Cuts at: 2253 4114 4481 2253
Size: 1861 367 4556
Fragments arranged by size:
4556 1861 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2326 2467 3036 4105 5560
Size: 325 217 627 539 141 569 1069 1455
Cuts at: 5560 618
Size: 1842
Fragments arranged by size:
1842 1455 1069 627 569 539 325 217
141
BanII G_rGCy'C
Cuts at: 1391 2207 2440 3206 4103 1391
Size: 816 233 766 897 4072
Fragments arranged by size:
4072 897 816 766 233
BbeI G_GCGC'C
Cuts at: 2330 2471 2330
Size: 141 6643
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2755 2865 3285 3312 3567 3843 928
Size: 971 856 110 420 27 255 276 3869
Fragments arranged by size:
3869 971 856 420 276 255 110 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4975 5009 1903
Size: 34 3038 34 3678
Fragments arranged by size:
3678 3038 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4676 5886 4676
Size: 1210 5574
BclI T'GATC_A
Cuts at: 1548 2841 3270 1548
Size: 1293 429 5062
Fragments arranged by size:
5062 1293 429
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5395 136
Size: 107 122 71 631 605 3723 1525
Fragments arranged by size:
3723 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3321 3357 6779 3321
Size: 36 3422 3326
Fragments arranged by size:
3422 3326 36
BlpI GC'TnA_GC
Cuts at: 4201 4201
Size: 6784
Bme1580I G_kGCm'C
Cuts at: 1790 2207 2440 4845 6001 1790
Size: 417 233 2405 1156 2573
Fragments arranged by size:
2573 2405 1156 417 233
BmgBI CAC'GTC
Cuts at: 1318 2852 3871 1318
Size: 1534 1019 4231
Fragments arranged by size:
4231 1534 1019
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2210 4515 5473
Size: 416 53 83 399 24 793 2305 958
Cuts at: 5473 442
Size: 1753
Fragments arranged by size:
2305 1753 958 793 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6784
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3901 3901
Size: 6784
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2681 2702 2780 3190 5444 1239
Size: 893 549 21 78 410 2254 2579
Fragments arranged by size:
2579 2254 893 549 410 78 21
Bpu10I CC'TnA_GC
Cuts at: 2048 2998 2048
Size: 950 5834
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2708 4909 5774 6072 2708
Size: 2201 865 298 3420
Fragments arranged by size:
3420 2201 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2763 3368 3386 3470 5447 882
Size: 617 1264 605 18 84 1977 2219
Fragments arranged by size:
2219 1977 1264 617 605 84 18
BsaAI yAC'GTr
Cuts at: 493 1204 4575 493
Size: 711 3371 2702
Fragments arranged by size:
3371 2702 711
BsaBI GATnn'nnATC
Cuts at: 3878 6778 3878
Size: 2900 3884
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2327 2468 3282 4591
Size: 53 83 186 936 794 141 814 1309
Cuts at: 4591 4973 5608 275
Size: 382 635 1451
Fragments arranged by size:
1451 1309 936 814 794 635 382 186
141 83 53
BsaWI w'CCGG_w
Cuts at: 4079 5217 5615 5889 6036 6447 4079
Size: 1138 398 274 147 411 4416
Fragments arranged by size:
4416 1138 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6559
Fragments arranged by size:
6559 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2258 2569 2599 3980 1186
Size: 910 162 311 30 1381 3990
Fragments arranged by size:
3990 1381 910 311 162 30
BseYI C'CCAG_C
Cuts at: 2130 2292 5987 6483 2130
Size: 162 3695 496 2431
Fragments arranged by size:
3695 2431 496 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3555 1163
Size: 573 1819 4392
Fragments arranged by size:
4392 1819 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4234 4995 6023 1540
Size: 275 186 2233 761 1028 2301
Fragments arranged by size:
2301 2233 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4845 4930 6001 4845
Size: 85 1071 5628
Fragments arranged by size:
5628 1071 85
BsmI GAATG_Cn'
Cuts at: 2810 4295 4388 2810
Size: 1485 93 5206
Fragments arranged by size:
5206 1485 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2726 2726
Size: 6784
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2207 2440 3206 4103 4845 4930 6001
Size: 399 417 233 766 897 742 85 1071
Cuts at: 6001 1391
Size: 2174
Fragments arranged by size:
2174 1071 897 766 742 417 399 233
85
BspEI T'CCGG_A
Cuts at: 6447 6447
Size: 6784
BspHI T'CATG_A
Cuts at: 3346 4673 6403 3346
Size: 1327 1730 3727
Fragments arranged by size:
3727 1730 1327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2878 4139 844
Size: 2034 1261 3489
Fragments arranged by size:
3489 2034 1261
BsrBI CCG'CTC
Cuts at: 1394 1823 2104 2542 2997 4671 1394
Size: 429 281 438 455 1674 3507
Fragments arranged by size:
3507 1674 455 438 429 281
BsrDI GCAATG_nn'
Cuts at: 65 1248 3775 5279 5453 65
Size: 1183 2527 1504 174 1396
Fragments arranged by size:
2527 1504 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3003 5428 5615 1700
Size: 110 1193 2425 187 2869
Fragments arranged by size:
2869 2425 1193 187 110
BsrGI T'GTAC_A
Cuts at: 96 2407 96
Size: 2311 4473
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6784
BssSI C'ACGA_G
Cuts at: 4844 5856 4844
Size: 1012 5772
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6784
BstBI TT'CG_AA
Cuts at: 5588 5588
Size: 6784
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6784
BstXI CCAn_nnnn'nTGG
Cuts at: 2799 3033 4050 2799
Size: 234 1017 5533
Fragments arranged by size:
5533 1017 234
BstYI r'GATC_y
Cuts at: 1972 2253 2677 2896 3321 3357 4114 4481 4866
Size: 281 424 219 425 36 757 367 385
Cuts at: 4866 4883 6335 6421 6433 6779 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 757 425 424 385 367 346
281 219 86 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2367 2367
Size: 6784
BtgI C'CryG_G
Cuts at: 513 1065 2004 2337 2913 3658 513
Size: 552 939 333 576 745 3639
Fragments arranged by size:
3639 939 745 576 552 333
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3807 487
Size: 683 56 2581 3464
Fragments arranged by size:
3464 2581 683 56
BtsI GCAGTG_nn'
Cuts at: 2463 2694 3306 3760 4300 5094 5114 2463
Size: 231 612 454 540 794 20 4133
Fragments arranged by size:
4133 794 612 540 454 231 20
ClaI AT'CG_AT
Cuts at: 1554 2394 1554
Size: 840 5944
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6749
DraI TTT'AAA
Cuts at: 4088 4088
Size: 6784
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5791 809
Size: 507 4475 1802
Fragments arranged by size:
4475 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2178 4231
Size: 54 1425 50 275 18 201 147 2053
Cuts at: 4231 5120 6749 8
Size: 889 1629 43
Fragments arranged by size:
2053 1629 1425 889 275 201 147 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4231 1537
Size: 275 2419 4090
Fragments arranged by size:
4090 2419 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2486 2496 2600 3585 3900 4061 4714 1353
Size: 1133 10 104 985 315 161 653 3423
Fragments arranged by size:
3423 1133 985 653 315 161 104 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2596 5371 5745 5891 387
Size: 1064 138 1007 2775 374 146 1280
Fragments arranged by size:
2775 1280 1064 1007 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2681 2702 2780 3190 3579 4019
Size: 393 500 549 21 78 410 389 440
Cuts at: 4019 4847 5444 6231 6499 1239
Size: 828 597 787 268 1524
Fragments arranged by size:
1524 828 787 597 549 500 440 410
393 389 268 78 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3125 3125
Size: 6784
EcoO109I rG'GnC_Cy
Cuts at: 2024 2204 2436 2437 3463 4039 2024
Size: 180 232 1 1026 576 4769
Fragments arranged by size:
4769 1026 576 232 180 1
EcoRI G'AATT_C
Cuts at: 4093 4471 4093
Size: 378 6406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2953 2953
Size: 6784
FspI TGC'GCA
Cuts at: 5290 5290
Size: 6784
HaeII r_GCGC'y
Cuts at: 1678 2140 2330 2471 5931 1678
Size: 462 190 141 3460 2531
Fragments arranged by size:
3460 2531 462 190 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2760 2792 5425 5457 5499
Size: 32 135 32 1188 32 2633 32 42
Cuts at: 5499 5531 1373
Size: 32 2626
Fragments arranged by size:
2633 2626 1188 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2485 3172 4128 4374 669
Size: 988 281 547 687 956 246 3079
Fragments arranged by size:
3079 988 956 687 547 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6784
HpaI GTT'AAC
Cuts at: 3172 4374 3172
Size: 1202 5582
KasI G'GCGC_C
Cuts at: 2326 2467 2326
Size: 141 6643
KpnI G_GTAC'C
Cuts at: 4109 4109
Size: 6784
MfeI C'AATT_G
Cuts at: 4383 4383
Size: 6784
MluI A'CGCG_T
Cuts at: 6660 6660
Size: 6784
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2134 5898 6082 1305
Size: 829 3764 184 2007
Fragments arranged by size:
3764 2007 829 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4962
Fragments arranged by size:
4962 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2516 2741 2963 4742 5101 5260 518
Size: 648 1350 225 222 1779 359 159 2042
Fragments arranged by size:
2042 1779 1350 648 359 225 222 159
MspA1I CmG'CkG
Cuts at: 1606 2119 2674 3033 3102 4200 4877 6025 6270
Size: 513 555 359 69 1098 677 1148 245
Cuts at: 6270 1606
Size: 2120
Fragments arranged by size:
2120 1148 1098 677 555 513 359 245
69
NaeI GCC'GGC
Cuts at: 3005 3005
Size: 6784
NarI GG'CG_CC
Cuts at: 2327 2468 2327
Size: 141 6643
NcoI C'CATG_G
Cuts at: 513 1065 2004 2913 3658 513
Size: 552 939 909 745 3639
Fragments arranged by size:
3639 939 909 745 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6784
NgoMIV G'CCGG_C
Cuts at: 3003 3003
Size: 6784
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6784
NotI GC'GGCC_GC
Cuts at: 4231 4231
Size: 6784
NspI r_CATG'y
Cuts at: 1132 1229 4142 5687 1132
Size: 97 2913 1545 2229
Fragments arranged by size:
2913 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6784
PciI A'CATG_T
Cuts at: 5683 5683
Size: 6784
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6784
PfoI T'CCnGG_A
Cuts at: 2148 2757 3206 2148
Size: 609 449 5726
Fragments arranged by size:
5726 609 449
PmeI GTTT'AAAC
Cuts at: 4088 4088
Size: 6784
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4853 4885 6366 6398 4853
Size: 32 1481 32 5239
Fragments arranged by size:
5239 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3463 4039 3463
Size: 576 6208
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6784
PsiI TTA'TAA
Cuts at: 4354 4354
Size: 6784
PspOMI G'GGCC_C
Cuts at: 2203 2436 2203
Size: 233 6551
PstI C_TGCA'G
Cuts at: 830 2655 2889 4136 830
Size: 1825 234 1247 3478
Fragments arranged by size:
3478 1825 1247 234
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6784
PvuII CAG'CTG
Cuts at: 2674 3102 2674
Size: 428 6356
SalI G'TCGA_C
Cuts at: 1936 4126 1936
Size: 2190 4594
SapI GCTCTTCn'nnn_
Cuts at: 2486 2486
Size: 6784
SbfI CC_TGCA'GG
Cuts at: 2655 4136 2655
Size: 1481 5303
ScaI AGT'ACT
Cuts at: 3818 5032 6744 3818
Size: 1214 1712 3858
Fragments arranged by size:
3858 1712 1214
SfcI C'TryA_G
Cuts at: 826 1045 2449 2651 2885 4132 5267 5948 6139
Size: 219 1404 202 234 1247 1135 681 191
Cuts at: 6139 826
Size: 1471
Fragments arranged by size:
1471 1404 1247 1135 681 234 219 202
191
SfoI GGC'GCC
Cuts at: 2328 2469 2328
Size: 141 6643
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6784
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6784
SmaI CCC'GGG
Cuts at: 1872 4111 1872
Size: 2239 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2240 2723 4888 5789 6051 820
Size: 197 926 297 483 2165 901 262 1553
Fragments arranged by size:
2165 1553 926 901 483 297 262 197
SnaBI TAC'GTA
Cuts at: 493 4575 493
Size: 4082 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6784
SphI G_CATG'C
Cuts at: 1132 4142 1132
Size: 3010 3774
SspI AAT'ATT
Cuts at: 5 52 4708 5
Size: 47 4656 2081
Fragments arranged by size:
4656 2081 47
StuI AGG'CCT
Cuts at: 1517 2372 1517
Size: 855 5929
StyI C'CwwG_G
Cuts at: 513 1065 2004 2913 3658 4223 513
Size: 552 939 909 745 565 3074
Fragments arranged by size:
3074 939 909 745 565 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4811 4828 4981 5166 5611 325
Size: 1255 3231 17 153 185 445 1498
Fragments arranged by size:
3231 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2407 2934 3816
Size: 274 80 33 51 1443 430 527 882
Cuts at: 3816 5030 6742 96
Size: 1214 1712 138
Fragments arranged by size:
1712 1443 1214 882 527 430 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6744 6776 6744
Size: 32 6752
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6784
XbaI T'CTAG_A
Cuts at: 4120 4120
Size: 6784
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2344 2344
Size: 6784
XhoI C'TCGA_G
Cuts at: 1943 2240 1943
Size: 297 6487
XmaI C'CCGG_G
Cuts at: 1870 4109 1870
Size: 2239 4545
XmnI GAAnn'nnTTC
Cuts at: 4913 4913
Size: 6784
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4592 276
Size: 53 83 186 936 3058 2468
Fragments arranged by size:
3058 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SrfI SwaI