Restriction map for
pFN21AB8690
(Circular) (Six-base) MAPSORT of: pFN21AB8690.seq Check: 1896 from: 1 to: 6538
pFN21AB8690 6538 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2709 2709
Size: 6538
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4348 278
Size: 53 83 186 936 2812 2468
Fragments arranged by size:
2812 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3881 1937
Size: 1944 4594
Acc65I G'GTAC_C
Cuts at: 3859 3859
Size: 6538
AclI AA'CG_TT
Cuts at: 1563 4665 5038 1563
Size: 3102 373 3063
Fragments arranged by size:
3102 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 4601 5985 6253 1632
Size: 464 2505 1384 268 1917
Fragments arranged by size:
2505 1917 1384 464 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6341
AflIII A'CryG_T
Cuts at: 1203 5437 6414 1203
Size: 4234 977 1327
Fragments arranged by size:
4234 1327 977
AgeI A'CCGG_T
Cuts at: 5369 5369
Size: 6538
AhdI GACnn_n'nnGTC
Cuts at: 3708 5267 3708
Size: 1559 4979
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6538
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6538
AlwNI CAG_nnn'CTG
Cuts at: 2019 2346 2419 2701 4281 5853 2019
Size: 327 73 282 1580 1572 2704
Fragments arranged by size:
2704 1580 1572 327 282 73
ApaI G_GGCC'C
Cuts at: 2126 2968 2126
Size: 842 5696
ApaLI G'TGCA_C
Cuts at: 3459 4595 5751 3459
Size: 1136 1156 4246
Fragments arranged by size:
4246 1156 1136
ApoI r'AATT_y
Cuts at: 1493 3847 4073 4225 6334 1493
Size: 2354 226 152 2109 1697
Fragments arranged by size:
2354 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5092 160
Size: 872 4060 1606
Fragments arranged by size:
4060 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6538
AvaI C'yCGr_G
Cuts at: 1870 1943 2126 2179 2453 2463 2579 2680 3061
Size: 73 183 53 274 10 116 101 381
Cuts at: 3061 3233 3246 3478 3863 1870
Size: 172 13 232 385 4545
Fragments arranged by size:
4545 385 381 274 232 183 172 116
101 73 53 13 10
BamHI G'GATC_C
Cuts at: 3868 4235 3868
Size: 367 6171
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2266 2605 2981 3073 3496
Size: 325 217 627 479 339 376 92 423
Cuts at: 3496 3859 5314 618
Size: 363 1455 1842
Fragments arranged by size:
1842 1455 627 479 423 376 363 339
325 217 92
BanII G_rGCy'C
Cuts at: 1391 2126 2802 2968 3485 3580 3614 3857 1391
Size: 735 676 166 517 95 34 243 4072
Fragments arranged by size:
4072 735 676 517 243 166 95 34
BbeI G_GCGC'C
Cuts at: 3077 3077
Size: 6538
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3762 928
Size: 971 1863 3704
Fragments arranged by size:
3704 1863 971
BbvCI CC'TCA_GC
Cuts at: 3651 3651
Size: 6538
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4729 4763 1903
Size: 34 2792 34 3678
Fragments arranged by size:
3678 2792 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4430 5640 4430
Size: 1210 5328
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6538
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2121 5149 136
Size: 107 122 71 631 605 449 3028 1525
Fragments arranged by size:
3028 1525 631 605 449 122 107 71
BglII A'GATC_T
Cuts at: 6533 6533
Size: 6538
BlpI GC'TnA_GC
Cuts at: 3308 3613 3955 3308
Size: 305 342 5891
Fragments arranged by size:
5891 342 305
Bme1580I G_kGCm'C
Cuts at: 1790 2126 2186 2652 2968 3463 3791 4599 5755
Size: 336 60 466 316 495 328 808 1156
Cuts at: 5755 1790
Size: 2573
Fragments arranged by size:
2573 1156 808 495 466 336 328 316
60
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6538
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4269 5227 442
Size: 416 53 83 399 24 2852 958 1753
Fragments arranged by size:
2852 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6538
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3047 3047
Size: 6538
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2044 2222 2243 2375 2579 2849 3335 3452
Size: 805 178 21 132 204 270 486 117
Cuts at: 3452 5198 1239
Size: 1746 2579
Fragments arranged by size:
2579 1746 805 486 270 204 178 132
117 21
Bpu10I CC'TnA_GC
Cuts at: 3651 3651
Size: 6538
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4663 5528 5826 4663
Size: 865 298 5375
Fragments arranged by size:
5375 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5201 882
Size: 617 3702 2219
Fragments arranged by size:
3702 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4329 493
Size: 711 3125 2702
Fragments arranged by size:
3125 2702 711
BsaBI GATnn'nnATC
Cuts at: 6532 6532
Size: 6538
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 2642 3074 3645
Size: 53 83 186 936 615 494 432 571
Cuts at: 3645 4345 4727 5362 275
Size: 700 382 635 1451
Fragments arranged by size:
1451 936 700 635 615 571 494 432
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3154 4971 5369 5643 5790 6201 3154
Size: 1817 398 274 147 411 3491
Fragments arranged by size:
3491 1817 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3512 3542 3548 3578 1178
Size: 30 165 30 2109 30 6 30 4138
Fragments arranged by size:
4138 2109 165 30 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2567 2783 2846 3025 3130 3449 3526 3652
Size: 1381 216 63 179 105 319 77 126
Cuts at: 3652 3739 1186
Size: 87 3985
Fragments arranged by size:
3985 1381 319 216 179 126 105 87
77 63
BseYI C'CCAG_C
Cuts at: 2522 3054 3144 3294 3792 5741 6237 2522
Size: 532 90 150 498 1949 496 2823
Fragments arranged by size:
2823 1949 532 498 496 150 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3263 3284 3848 1163
Size: 573 1527 21 564 3853
Fragments arranged by size:
3853 1527 573 564 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2112 3988 4749 5777 1540
Size: 275 186 111 1876 761 1028 2301
Fragments arranged by size:
2301 1876 1028 761 275 186 111
BsiHKAI G_wGCw'C
Cuts at: 2472 2517 2802 3463 4599 4684 5755 2472
Size: 45 285 661 1136 85 1071 3255
Fragments arranged by size:
3255 1136 1071 661 285 85 45
BsmI GAATG_Cn'
Cuts at: 4049 4142 4049
Size: 93 6445
BsmBI CGTCTCn'nnnn_
Cuts at: 3202 3202
Size: 6538
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2186 2472 2517 2652 2802 2968
Size: 399 336 60 286 45 135 150 166
Cuts at: 2968 3463 3485 3580 3614 3791 3857 4599 4684
Size: 495 22 95 34 177 66 742 85
Cuts at: 4684 5755 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 495 399 336 286 177
166 150 135 95 85 66 60 45
34 22
BspEI T'CCGG_A
Cuts at: 6201 6201
Size: 6538
BspHI T'CATG_A
Cuts at: 4427 6157 4427
Size: 1730 4808
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2709 2995 3893 844
Size: 1865 286 898 3489
Fragments arranged by size:
3489 1865 898 286
BsrBI CCG'CTC
Cuts at: 1394 1823 2452 2853 3596 4425 1394
Size: 429 629 401 743 829 3507
Fragments arranged by size:
3507 829 743 629 429 401
BsrDI GCAATG_nn'
Cuts at: 65 1248 5033 5207 65
Size: 1183 3785 174 1396
Fragments arranged by size:
3785 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2111 2724 3225 5182 5369 1700
Size: 110 301 613 501 1957 187 2869
Fragments arranged by size:
2869 1957 613 501 301 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6538
BssHII G'CGCG_C
Cuts at: 1925 2097 1925
Size: 172 6366
BssSI C'ACGA_G
Cuts at: 4598 5610 4598
Size: 1012 5526
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2701 794
Size: 1907 4631
BstBI TT'CG_AA
Cuts at: 5342 5342
Size: 6538
BstEII G'GTnAC_C
Cuts at: 1184 3228 1184
Size: 2044 4494
BstXI CCAn_nnnn'nTGG
Cuts at: 2893 2893
Size: 6538
BstYI r'GATC_y
Cuts at: 1972 2847 3868 4235 4620 4637 6089 6175 6187
Size: 875 1021 367 385 17 1452 86 12
Cuts at: 6187 6533 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1021 875 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2028 3037 3807 2028
Size: 1009 770 4759
Fragments arranged by size:
4759 1009 770
BtgI C'CryG_G
Cuts at: 513 1065 2004 2389 2588 3129 3259 513
Size: 552 939 385 199 541 130 3792
Fragments arranged by size:
3792 939 552 541 385 199 130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5799
Fragments arranged by size:
5799 683 56
BtsI GCAGTG_nn'
Cuts at: 2492 2671 2723 3022 3220 3275 3836 4054 4848
Size: 179 52 299 198 55 561 218 794
Cuts at: 4848 4868 2492
Size: 20 4162
Fragments arranged by size:
4162 794 561 299 218 198 179 55
52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6538
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6503
DraI TTT'AAA
Cuts at: 3842 3842
Size: 6538
DraIII CAC_nnn'GTG
Cuts at: 2810 2810
Size: 6538
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5545 809
Size: 507 4229 1802
Fragments arranged by size:
4229 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2754 3019
Size: 54 1425 50 275 18 279 645 265
Cuts at: 3019 3187 3985 4874 6503 8
Size: 168 798 889 1629 43
Fragments arranged by size:
1629 1425 889 798 645 279 275 265
168 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2109 3985 1537
Size: 275 297 1876 4090
Fragments arranged by size:
4090 1876 297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2171 4468 1353
Size: 818 2297 3423
Fragments arranged by size:
3423 2297 818
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2275 3057 5125 5499 5645 387
Size: 1064 138 686 782 2068 374 146 1280
Fragments arranged by size:
2068 1280 1064 782 686 374 146 138
EcoICRI GAG'CTC
Cuts at: 2800 2800
Size: 6538
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2044 2096 2222 2243 2375 2579 2849
Size: 393 412 52 126 21 132 204 270
Cuts at: 2849 3335 3452 4601 5198 5985 6253 1239
Size: 486 117 1149 597 787 268 1524
Fragments arranged by size:
1524 1149 787 597 486 412 393 270
268 204 132 126 117 52 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3667 3667
Size: 6538
EcoO109I rG'GnC_Cy
Cuts at: 2032 2123 2460 2870 2964 2965 3427 3733 3803
Size: 91 337 410 94 1 462 306 70
Cuts at: 3803 2032
Size: 4767
Fragments arranged by size:
4767 462 410 337 306 94 91 70
1
EcoRI G'AATT_C
Cuts at: 3847 4225 3847
Size: 378 6160
FseI GG_CCGG'CC
Cuts at: 2115 2115
Size: 6538
FspI TGC'GCA
Cuts at: 5044 5044
Size: 6538
HaeII r_GCGC'y
Cuts at: 1678 2596 2620 3077 5685 1678
Size: 918 24 457 2608 2531
Fragments arranged by size:
2608 2531 918 457 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3512 3544 3694 3698 3726
Size: 32 135 32 1940 32 150 4 28
Cuts at: 3726 3730 3752 3784 5179 5211 5253 5285 1373
Size: 4 22 32 1395 32 42 32 2626
Fragments arranged by size:
2626 1940 1395 150 135 42 32 32
32 32 32 32 28 22 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3882 4128 669
Size: 988 281 1944 246 3079
Fragments arranged by size:
3079 1944 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6538
HpaI GTT'AAC
Cuts at: 4128 4128
Size: 6538
KasI G'GCGC_C
Cuts at: 3073 3073
Size: 6538
KpnI G_GTAC'C
Cuts at: 3863 3863
Size: 6538
MfeI C'AATT_G
Cuts at: 4137 4137
Size: 6538
MluI A'CGCG_T
Cuts at: 6414 6414
Size: 6538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5652 5836 1305
Size: 4347 184 2007
Fragments arranged by size:
4347 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2756 10
Size: 54 1425 343 924 3792
Fragments arranged by size:
3792 1425 924 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2891 4496 4855 5014 518
Size: 648 1725 1605 359 159 2042
Fragments arranged by size:
2042 1725 1605 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2428 2544 2590 2974 3054 3261 3298 3548
Size: 822 116 46 384 80 207 37 250
Cuts at: 3548 3954 4631 5779 6024 1606
Size: 406 677 1148 245 2120
Fragments arranged by size:
2120 1148 822 677 406 384 250 245
207 116 80 46 37
NaeI GCC'GGC
Cuts at: 2113 2113
Size: 6538
NarI GG'CG_CC
Cuts at: 3074 3074
Size: 6538
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5047
Fragments arranged by size:
5047 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6538
NgoMIV G'CCGG_C
Cuts at: 2111 2111
Size: 6538
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6538
NotI GC'GGCC_GC
Cuts at: 3985 3985
Size: 6538
NspI r_CATG'y
Cuts at: 1132 1229 3896 5441 1132
Size: 97 2667 1545 2229
Fragments arranged by size:
2667 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2996 1348
Size: 1648 4890
PciI A'CATG_T
Cuts at: 5437 5437
Size: 6538
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6538
PfoI T'CCnGG_A
Cuts at: 2193 2193
Size: 6538
PmeI GTTT'AAAC
Cuts at: 3842 3842
Size: 6538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4607 4639 6120 6152 4607
Size: 32 1481 32 4993
Fragments arranged by size:
4993 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2460 3733 2460
Size: 1273 5265
PshAI GACnn'nnGTC
Cuts at: 1613 2919 1613
Size: 1306 5232
PsiI TTA'TAA
Cuts at: 4108 4108
Size: 6538
PspOMI G'GGCC_C
Cuts at: 2122 2964 2122
Size: 842 5696
PspXI vC'TCGA_Gb
Cuts at: 2453 2680 3478 2453
Size: 227 798 5513
Fragments arranged by size:
5513 798 227
PstI C_TGCA'G
Cuts at: 830 2697 3585 3890 830
Size: 1867 888 305 3478
Fragments arranged by size:
3478 1867 888 305
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6538
PvuII CAG'CTG
Cuts at: 3054 3298 3054
Size: 244 6294
SacI G_AGCT'C
Cuts at: 2802 2802
Size: 6538
SacII CC_GC'GG
Cuts at: 2591 3262 2591
Size: 671 5867
SalI G'TCGA_C
Cuts at: 1936 3880 1936
Size: 1944 4594
SanDI GG'GwC_CC
Cuts at: 2460 3733 2460
Size: 1273 5265
SapI GCTCTTCn'nnn_
Cuts at: 2171 2171
Size: 6538
SbfI CC_TGCA'GG
Cuts at: 3890 3890
Size: 6538
ScaI AGT'ACT
Cuts at: 4786 6498 4786
Size: 1712 4826
SfcI C'TryA_G
Cuts at: 826 1045 2693 3581 3886 5021 5702 5893 826
Size: 219 1648 888 305 1135 681 191 1471
Fragments arranged by size:
1648 1471 1135 888 681 305 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2121 2121
Size: 6538
SfoI GGC'GCC
Cuts at: 3075 3075
Size: 6538
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6538
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6538
SmaI CCC'GGG
Cuts at: 1872 2181 3235 3248 3865 1872
Size: 309 1054 13 617 4545
Fragments arranged by size:
4545 1054 617 309 13
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2453 2680 3478 4642 5543 5805
Size: 197 926 510 227 798 1164 901 262
Cuts at: 5805 820
Size: 1553
Fragments arranged by size:
1553 1164 926 901 798 510 262 227
197
SnaBI TAC'GTA
Cuts at: 493 4329 493
Size: 3836 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6538
SphI G_CATG'C
Cuts at: 1132 3896 1132
Size: 2764 3774
SrfI GCCC'GGGC
Cuts at: 3248 3248
Size: 6538
SspI AAT'ATT
Cuts at: 5 52 4462 5
Size: 47 4410 2081
Fragments arranged by size:
4410 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6538
StyI C'CwwG_G
Cuts at: 513 1065 2004 2304 2751 3618 3977 513
Size: 552 939 300 447 867 359 3074
Fragments arranged by size:
3074 939 867 552 447 359 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2966 3215 3702 4565 4582 4735 4920
Size: 1255 1386 249 487 863 17 153 185
Cuts at: 4920 5365 325
Size: 445 1498
Fragments arranged by size:
1498 1386 1255 863 487 445 249 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2376 4784 6496
Size: 274 80 33 51 1443 399 2408 1712
Cuts at: 6496 96
Size: 138
Fragments arranged by size:
2408 1712 1443 399 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2277 2309 3050 3082 6498 6530 2277
Size: 32 741 32 3416 32 2285
Fragments arranged by size:
3416 2285 741 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2641 3766 1316
Size: 1325 1125 4088
Fragments arranged by size:
4088 1325 1125
XbaI T'CTAG_A
Cuts at: 3874 3874
Size: 6538
XhoI C'TCGA_G
Cuts at: 1943 2453 2680 3478 1943
Size: 510 227 798 5003
Fragments arranged by size:
5003 798 510 227
XmaI C'CCGG_G
Cuts at: 1870 2179 3233 3246 3863 1870
Size: 309 1054 13 617 4545
Fragments arranged by size:
4545 1054 617 309 13
XmnI GAAnn'nnTTC
Cuts at: 4667 4667
Size: 6538
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4346 276
Size: 53 83 186 936 2812 2468
Fragments arranged by size:
2812 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
EcoRV FalI FspAI NruI NsiI PacI PmlI PsrI
RsrII SexAI SwaI XcmI