(Circular) (Six-base) MAPSORT of: pFN21AB8690.seq Check: 1896 from: 1 to: 6538 pFN21AB8690 6538 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2709 2709 Size: 6538 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4348 278 Size: 53 83 186 936 2812 2468 Fragments arranged by size: 2812 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3881 1937 Size: 1944 4594 Acc65I G'GTAC_C Cuts at: 3859 3859 Size: 6538 AclI AA'CG_TT Cuts at: 1563 4665 5038 1563 Size: 3102 373 3063 Fragments arranged by size: 3102 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 4601 5985 6253 1632 Size: 464 2505 1384 268 1917 Fragments arranged by size: 2505 1917 1384 464 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6341 AflIII A'CryG_T Cuts at: 1203 5437 6414 1203 Size: 4234 977 1327 Fragments arranged by size: 4234 1327 977 AgeI A'CCGG_T Cuts at: 5369 5369 Size: 6538 AhdI GACnn_n'nnGTC Cuts at: 3708 5267 3708 Size: 1559 4979 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6538 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6538 AlwNI CAG_nnn'CTG Cuts at: 2019 2346 2419 2701 4281 5853 2019 Size: 327 73 282 1580 1572 2704 Fragments arranged by size: 2704 1580 1572 327 282 73 ApaI G_GGCC'C Cuts at: 2126 2968 2126 Size: 842 5696 ApaLI G'TGCA_C Cuts at: 3459 4595 5751 3459 Size: 1136 1156 4246 Fragments arranged by size: 4246 1156 1136 ApoI r'AATT_y Cuts at: 1493 3847 4073 4225 6334 1493 Size: 2354 226 152 2109 1697 Fragments arranged by size: 2354 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5092 160 Size: 872 4060 1606 Fragments arranged by size: 4060 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6538 AvaI C'yCGr_G Cuts at: 1870 1943 2126 2179 2453 2463 2579 2680 3061 Size: 73 183 53 274 10 116 101 381 Cuts at: 3061 3233 3246 3478 3863 1870 Size: 172 13 232 385 4545 Fragments arranged by size: 4545 385 381 274 232 183 172 116 101 73 53 13 10 BamHI G'GATC_C Cuts at: 3868 4235 3868 Size: 367 6171 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2266 2605 2981 3073 3496 Size: 325 217 627 479 339 376 92 423 Cuts at: 3496 3859 5314 618 Size: 363 1455 1842 Fragments arranged by size: 1842 1455 627 479 423 376 363 339 325 217 92 BanII G_rGCy'C Cuts at: 1391 2126 2802 2968 3485 3580 3614 3857 1391 Size: 735 676 166 517 95 34 243 4072 Fragments arranged by size: 4072 735 676 517 243 166 95 34 BbeI G_GCGC'C Cuts at: 3077 3077 Size: 6538 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3762 928 Size: 971 1863 3704 Fragments arranged by size: 3704 1863 971 BbvCI CC'TCA_GC Cuts at: 3651 3651 Size: 6538 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4729 4763 1903 Size: 34 2792 34 3678 Fragments arranged by size: 3678 2792 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4430 5640 4430 Size: 1210 5328 BclI T'GATC_A Cuts at: 1548 1548 Size: 6538 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2121 5149 136 Size: 107 122 71 631 605 449 3028 1525 Fragments arranged by size: 3028 1525 631 605 449 122 107 71 BglII A'GATC_T Cuts at: 6533 6533 Size: 6538 BlpI GC'TnA_GC Cuts at: 3308 3613 3955 3308 Size: 305 342 5891 Fragments arranged by size: 5891 342 305 Bme1580I G_kGCm'C Cuts at: 1790 2126 2186 2652 2968 3463 3791 4599 5755 Size: 336 60 466 316 495 328 808 1156 Cuts at: 5755 1790 Size: 2573 Fragments arranged by size: 2573 1156 808 495 466 336 328 316 60 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6538 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4269 5227 442 Size: 416 53 83 399 24 2852 958 1753 Fragments arranged by size: 2852 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6538 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3047 3047 Size: 6538 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2044 2222 2243 2375 2579 2849 3335 3452 Size: 805 178 21 132 204 270 486 117 Cuts at: 3452 5198 1239 Size: 1746 2579 Fragments arranged by size: 2579 1746 805 486 270 204 178 132 117 21 Bpu10I CC'TnA_GC Cuts at: 3651 3651 Size: 6538 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4663 5528 5826 4663 Size: 865 298 5375 Fragments arranged by size: 5375 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5201 882 Size: 617 3702 2219 Fragments arranged by size: 3702 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4329 493 Size: 711 3125 2702 Fragments arranged by size: 3125 2702 711 BsaBI GATnn'nnATC Cuts at: 6532 6532 Size: 6538 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2148 2642 3074 3645 Size: 53 83 186 936 615 494 432 571 Cuts at: 3645 4345 4727 5362 275 Size: 700 382 635 1451 Fragments arranged by size: 1451 936 700 635 615 571 494 432 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3154 4971 5369 5643 5790 6201 3154 Size: 1817 398 274 147 411 3491 Fragments arranged by size: 3491 1817 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3512 3542 3548 3578 1178 Size: 30 165 30 2109 30 6 30 4138 Fragments arranged by size: 4138 2109 165 30 30 30 30 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2567 2783 2846 3025 3130 3449 3526 3652 Size: 1381 216 63 179 105 319 77 126 Cuts at: 3652 3739 1186 Size: 87 3985 Fragments arranged by size: 3985 1381 319 216 179 126 105 87 77 63 BseYI C'CCAG_C Cuts at: 2522 3054 3144 3294 3792 5741 6237 2522 Size: 532 90 150 498 1949 496 2823 Fragments arranged by size: 2823 1949 532 498 496 150 90 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3263 3284 3848 1163 Size: 573 1527 21 564 3853 Fragments arranged by size: 3853 1527 573 564 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2112 3988 4749 5777 1540 Size: 275 186 111 1876 761 1028 2301 Fragments arranged by size: 2301 1876 1028 761 275 186 111 BsiHKAI G_wGCw'C Cuts at: 2472 2517 2802 3463 4599 4684 5755 2472 Size: 45 285 661 1136 85 1071 3255 Fragments arranged by size: 3255 1136 1071 661 285 85 45 BsmI GAATG_Cn' Cuts at: 4049 4142 4049 Size: 93 6445 BsmBI CGTCTCn'nnnn_ Cuts at: 3202 3202 Size: 6538 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 2186 2472 2517 2652 2802 2968 Size: 399 336 60 286 45 135 150 166 Cuts at: 2968 3463 3485 3580 3614 3791 3857 4599 4684 Size: 495 22 95 34 177 66 742 85 Cuts at: 4684 5755 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 495 399 336 286 177 166 150 135 95 85 66 60 45 34 22 BspEI T'CCGG_A Cuts at: 6201 6201 Size: 6538 BspHI T'CATG_A Cuts at: 4427 6157 4427 Size: 1730 4808 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2709 2995 3893 844 Size: 1865 286 898 3489 Fragments arranged by size: 3489 1865 898 286 BsrBI CCG'CTC Cuts at: 1394 1823 2452 2853 3596 4425 1394 Size: 429 629 401 743 829 3507 Fragments arranged by size: 3507 829 743 629 429 401 BsrDI GCAATG_nn' Cuts at: 65 1248 5033 5207 65 Size: 1183 3785 174 1396 Fragments arranged by size: 3785 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2111 2724 3225 5182 5369 1700 Size: 110 301 613 501 1957 187 2869 Fragments arranged by size: 2869 1957 613 501 301 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6538 BssHII G'CGCG_C Cuts at: 1925 2097 1925 Size: 172 6366 BssSI C'ACGA_G Cuts at: 4598 5610 4598 Size: 1012 5526 BstAPI GCAn_nnn'nTGC Cuts at: 794 2701 794 Size: 1907 4631 BstBI TT'CG_AA Cuts at: 5342 5342 Size: 6538 BstEII G'GTnAC_C Cuts at: 1184 3228 1184 Size: 2044 4494 BstXI CCAn_nnnn'nTGG Cuts at: 2893 2893 Size: 6538 BstYI r'GATC_y Cuts at: 1972 2847 3868 4235 4620 4637 6089 6175 6187 Size: 875 1021 367 385 17 1452 86 12 Cuts at: 6187 6533 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1021 875 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2028 3037 3807 2028 Size: 1009 770 4759 Fragments arranged by size: 4759 1009 770 BtgI C'CryG_G Cuts at: 513 1065 2004 2389 2588 3129 3259 513 Size: 552 939 385 199 541 130 3792 Fragments arranged by size: 3792 939 552 541 385 199 130 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5799 Fragments arranged by size: 5799 683 56 BtsI GCAGTG_nn' Cuts at: 2492 2671 2723 3022 3220 3275 3836 4054 4848 Size: 179 52 299 198 55 561 218 794 Cuts at: 4848 4868 2492 Size: 20 4162 Fragments arranged by size: 4162 794 561 299 218 198 179 55 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6538 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6503 DraI TTT'AAA Cuts at: 3842 3842 Size: 6538 DraIII CAC_nnn'GTG Cuts at: 2810 2810 Size: 6538 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5545 809 Size: 507 4229 1802 Fragments arranged by size: 4229 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 2754 3019 Size: 54 1425 50 275 18 279 645 265 Cuts at: 3019 3187 3985 4874 6503 8 Size: 168 798 889 1629 43 Fragments arranged by size: 1629 1425 889 798 645 279 275 265 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2109 3985 1537 Size: 275 297 1876 4090 Fragments arranged by size: 4090 1876 297 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2171 4468 1353 Size: 818 2297 3423 Fragments arranged by size: 3423 2297 818 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2275 3057 5125 5499 5645 387 Size: 1064 138 686 782 2068 374 146 1280 Fragments arranged by size: 2068 1280 1064 782 686 374 146 138 EcoICRI GAG'CTC Cuts at: 2800 2800 Size: 6538 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2044 2096 2222 2243 2375 2579 2849 Size: 393 412 52 126 21 132 204 270 Cuts at: 2849 3335 3452 4601 5198 5985 6253 1239 Size: 486 117 1149 597 787 268 1524 Fragments arranged by size: 1524 1149 787 597 486 412 393 270 268 204 132 126 117 52 21 EcoNI CCTnn'n_nnAGG Cuts at: 3667 3667 Size: 6538 EcoO109I rG'GnC_Cy Cuts at: 2032 2123 2460 2870 2964 2965 3427 3733 3803 Size: 91 337 410 94 1 462 306 70 Cuts at: 3803 2032 Size: 4767 Fragments arranged by size: 4767 462 410 337 306 94 91 70 1 EcoRI G'AATT_C Cuts at: 3847 4225 3847 Size: 378 6160 FseI GG_CCGG'CC Cuts at: 2115 2115 Size: 6538 FspI TGC'GCA Cuts at: 5044 5044 Size: 6538 HaeII r_GCGC'y Cuts at: 1678 2596 2620 3077 5685 1678 Size: 918 24 457 2608 2531 Fragments arranged by size: 2608 2531 918 457 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3512 3544 3694 3698 3726 Size: 32 135 32 1940 32 150 4 28 Cuts at: 3726 3730 3752 3784 5179 5211 5253 5285 1373 Size: 4 22 32 1395 32 42 32 2626 Fragments arranged by size: 2626 1940 1395 150 135 42 32 32 32 32 32 32 28 22 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 3882 4128 669 Size: 988 281 1944 246 3079 Fragments arranged by size: 3079 1944 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6538 HpaI GTT'AAC Cuts at: 4128 4128 Size: 6538 KasI G'GCGC_C Cuts at: 3073 3073 Size: 6538 KpnI G_GTAC'C Cuts at: 3863 3863 Size: 6538 MfeI C'AATT_G Cuts at: 4137 4137 Size: 6538 MluI A'CGCG_T Cuts at: 6414 6414 Size: 6538 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5652 5836 1305 Size: 4347 184 2007 Fragments arranged by size: 4347 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2756 10 Size: 54 1425 343 924 3792 Fragments arranged by size: 3792 1425 924 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2891 4496 4855 5014 518 Size: 648 1725 1605 359 159 2042 Fragments arranged by size: 2042 1725 1605 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2428 2544 2590 2974 3054 3261 3298 3548 Size: 822 116 46 384 80 207 37 250 Cuts at: 3548 3954 4631 5779 6024 1606 Size: 406 677 1148 245 2120 Fragments arranged by size: 2120 1148 822 677 406 384 250 245 207 116 80 46 37 NaeI GCC'GGC Cuts at: 2113 2113 Size: 6538 NarI GG'CG_CC Cuts at: 3074 3074 Size: 6538 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5047 Fragments arranged by size: 5047 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6538 NgoMIV G'CCGG_C Cuts at: 2111 2111 Size: 6538 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6538 NotI GC'GGCC_GC Cuts at: 3985 3985 Size: 6538 NspI r_CATG'y Cuts at: 1132 1229 3896 5441 1132 Size: 97 2667 1545 2229 Fragments arranged by size: 2667 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2996 1348 Size: 1648 4890 PciI A'CATG_T Cuts at: 5437 5437 Size: 6538 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6538 PfoI T'CCnGG_A Cuts at: 2193 2193 Size: 6538 PmeI GTTT'AAAC Cuts at: 3842 3842 Size: 6538 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4607 4639 6120 6152 4607 Size: 32 1481 32 4993 Fragments arranged by size: 4993 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2460 3733 2460 Size: 1273 5265 PshAI GACnn'nnGTC Cuts at: 1613 2919 1613 Size: 1306 5232 PsiI TTA'TAA Cuts at: 4108 4108 Size: 6538 PspOMI G'GGCC_C Cuts at: 2122 2964 2122 Size: 842 5696 PspXI vC'TCGA_Gb Cuts at: 2453 2680 3478 2453 Size: 227 798 5513 Fragments arranged by size: 5513 798 227 PstI C_TGCA'G Cuts at: 830 2697 3585 3890 830 Size: 1867 888 305 3478 Fragments arranged by size: 3478 1867 888 305 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6538 PvuII CAG'CTG Cuts at: 3054 3298 3054 Size: 244 6294 SacI G_AGCT'C Cuts at: 2802 2802 Size: 6538 SacII CC_GC'GG Cuts at: 2591 3262 2591 Size: 671 5867 SalI G'TCGA_C Cuts at: 1936 3880 1936 Size: 1944 4594 SanDI GG'GwC_CC Cuts at: 2460 3733 2460 Size: 1273 5265 SapI GCTCTTCn'nnn_ Cuts at: 2171 2171 Size: 6538 SbfI CC_TGCA'GG Cuts at: 3890 3890 Size: 6538 ScaI AGT'ACT Cuts at: 4786 6498 4786 Size: 1712 4826 SfcI C'TryA_G Cuts at: 826 1045 2693 3581 3886 5021 5702 5893 826 Size: 219 1648 888 305 1135 681 191 1471 Fragments arranged by size: 1648 1471 1135 888 681 305 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2121 2121 Size: 6538 SfoI GGC'GCC Cuts at: 3075 3075 Size: 6538 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6538 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6538 SmaI CCC'GGG Cuts at: 1872 2181 3235 3248 3865 1872 Size: 309 1054 13 617 4545 Fragments arranged by size: 4545 1054 617 309 13 SmlI C'TyrA_G Cuts at: 820 1017 1943 2453 2680 3478 4642 5543 5805 Size: 197 926 510 227 798 1164 901 262 Cuts at: 5805 820 Size: 1553 Fragments arranged by size: 1553 1164 926 901 798 510 262 227 197 SnaBI TAC'GTA Cuts at: 493 4329 493 Size: 3836 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6538 SphI G_CATG'C Cuts at: 1132 3896 1132 Size: 2764 3774 SrfI GCCC'GGGC Cuts at: 3248 3248 Size: 6538 SspI AAT'ATT Cuts at: 5 52 4462 5 Size: 47 4410 2081 Fragments arranged by size: 4410 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6538 StyI C'CwwG_G Cuts at: 513 1065 2004 2304 2751 3618 3977 513 Size: 552 939 300 447 867 359 3074 Fragments arranged by size: 3074 939 867 552 447 359 300 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2966 3215 3702 4565 4582 4735 4920 Size: 1255 1386 249 487 863 17 153 185 Cuts at: 4920 5365 325 Size: 445 1498 Fragments arranged by size: 1498 1386 1255 863 487 445 249 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2376 4784 6496 Size: 274 80 33 51 1443 399 2408 1712 Cuts at: 6496 96 Size: 138 Fragments arranged by size: 2408 1712 1443 399 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2277 2309 3050 3082 6498 6530 2277 Size: 32 741 32 3416 32 2285 Fragments arranged by size: 3416 2285 741 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2641 3766 1316 Size: 1325 1125 4088 Fragments arranged by size: 4088 1325 1125 XbaI T'CTAG_A Cuts at: 3874 3874 Size: 6538 XhoI C'TCGA_G Cuts at: 1943 2453 2680 3478 1943 Size: 510 227 798 5003 Fragments arranged by size: 5003 798 510 227 XmaI C'CCGG_G Cuts at: 1870 2179 3233 3246 3863 1870 Size: 309 1054 13 617 4545 Fragments arranged by size: 4545 1054 617 309 13 XmnI GAAnn'nnTTC Cuts at: 4667 4667 Size: 6538 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4346 276 Size: 53 83 186 936 2812 2468 Fragments arranged by size: 2812 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PmlI PsrI RsrII SexAI SwaI XcmI