Restriction map for
pFN21AB8687
(Circular) (Six-base) MAPSORT of: pFN21AB8687.seq Check: 1009 from: 1 to: 6649
pFN21AB8687 6649 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4459 278
Size: 53 83 186 936 2923 2468
Fragments arranged by size:
2923 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3992 1937
Size: 2055 4594
Acc65I G'GTAC_C
Cuts at: 3970 3970
Size: 6649
AclI AA'CG_TT
Cuts at: 1563 4776 5149 1563
Size: 3213 373 3063
Fragments arranged by size:
3213 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3185 4712 6096 6364 1632
Size: 1553 1527 1384 268 1917
Fragments arranged by size:
1917 1553 1527 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6452
AflIII A'CryG_T
Cuts at: 1203 3676 5548 6525 1203
Size: 2473 1872 977 1327
Fragments arranged by size:
2473 1872 1327 977
AgeI A'CCGG_T
Cuts at: 5480 5480
Size: 6649
AhdI GACnn_n'nnGTC
Cuts at: 3659 5378 3659
Size: 1719 4930
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6649
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2378 1184
Size: 1194 5455
AlwNI CAG_nnn'CTG
Cuts at: 2123 4392 5964 2123
Size: 2269 1572 2808
Fragments arranged by size:
2808 2269 1572
ApaLI G'TGCA_C
Cuts at: 4706 5862 4706
Size: 1156 5493
ApoI r'AATT_y
Cuts at: 1493 2343 2662 3958 4184 4336 6445 1493
Size: 850 319 1296 226 152 2109 1697
Fragments arranged by size:
2109 1697 1296 850 319 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3813 5203 160
Size: 872 2781 1390 1606
Fragments arranged by size:
2781 1606 1390 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 2979 3974 1870
Size: 73 133 903 995 4545
Fragments arranged by size:
4545 995 903 133 73
BamHI G'GATC_C
Cuts at: 3979 4346 3979
Size: 367 6282
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2162 3970 5425 618
Size: 325 217 627 375 1808 1455 1842
Fragments arranged by size:
1842 1808 1455 627 375 325 217
BanII G_rGCy'C
Cuts at: 1391 2026 3968 1391
Size: 635 1942 4072
Fragments arranged by size:
4072 1942 635
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3472 928
Size: 971 1573 4105
Fragments arranged by size:
4105 1573 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4840 4874 1903
Size: 34 2903 34 3678
Fragments arranged by size:
3678 2903 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4541 5751 4541
Size: 1210 5439
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6649
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5260 136
Size: 107 122 71 631 605 3588 1525
Fragments arranged by size:
3588 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2845 6644 2845
Size: 3799 2850
BlpI GC'TnA_GC
Cuts at: 4066 4066
Size: 6649
Bme1580I G_kGCm'C
Cuts at: 1790 4710 5866 1790
Size: 2920 1156 2573
Fragments arranged by size:
2920 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6649
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4380 5338 442
Size: 416 53 83 399 24 2963 958 1753
Fragments arranged by size:
2963 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2176 5309 1239
Size: 937 3133 2579
Fragments arranged by size:
3133 2579 937
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3909 4774 5639 5937 3909
Size: 865 865 298 4621
Fragments arranged by size:
4621 865 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5312 882
Size: 617 3813 2219
Fragments arranged by size:
3813 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2040 3718 4440 493
Size: 711 836 1678 722 2702
Fragments arranged by size:
2702 1678 836 722 711
BsaBI GATnn'nnATC
Cuts at: 2523 6643 2523
Size: 4120 2529
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2739 4456 4838 5473
Size: 53 83 186 936 1206 1717 382 635
Cuts at: 5473 275
Size: 1451
Fragments arranged by size:
1717 1451 1206 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5082 5480 5754 5901 6312 5082
Size: 398 274 147 411 5419
Fragments arranged by size:
5419 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3031 3061 3742 3772 1178
Size: 30 165 30 1628 30 681 30 4055
Fragments arranged by size:
4055 1628 681 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2034 2104 2117 3863 1186
Size: 848 70 13 1746 3972
Fragments arranged by size:
3972 1746 848 70 13
BseYI C'CCAG_C
Cuts at: 2927 5852 6348 2927
Size: 2925 496 3228
Fragments arranged by size:
3228 2925 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2710 1163
Size: 573 974 5102
Fragments arranged by size:
5102 974 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2257 4099 4860 5888 1540
Size: 275 186 256 1842 761 1028 2301
Fragments arranged by size:
2301 1842 1028 761 275 256 186
BsiHKAI G_wGCw'C
Cuts at: 2026 4710 4795 5866 2026
Size: 2684 85 1071 2809
Fragments arranged by size:
2809 2684 1071 85
BsmI GAATG_Cn'
Cuts at: 2435 2447 4160 4253 2435
Size: 12 1713 93 4831
Fragments arranged by size:
4831 1713 93 12
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 3968 4710 4795 5866 1391
Size: 399 236 1942 742 85 1071 2174
Fragments arranged by size:
2174 1942 1071 742 399 236 85
BspEI T'CCGG_A
Cuts at: 6312 6312
Size: 6649
BspHI T'CATG_A
Cuts at: 4538 6268 4538
Size: 1730 4919
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2732 2774 4004 844
Size: 1888 42 1230 3489
Fragments arranged by size:
3489 1888 1230 42
BsrBI CCG'CTC
Cuts at: 1394 1823 4536 1394
Size: 429 2713 3507
Fragments arranged by size:
3507 2713 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2367 5144 5318 65
Size: 1183 1119 2777 174 1396
Fragments arranged by size:
2777 1396 1183 1119 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2095 5293 5480 1700
Size: 110 285 3198 187 2869
Fragments arranged by size:
3198 2869 285 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6649
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6649
BssSI C'ACGA_G
Cuts at: 2198 4709 5721 2198
Size: 2511 1012 3126
Fragments arranged by size:
3126 2511 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6649
BstBI TT'CG_AA
Cuts at: 5453 5453
Size: 6649
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6649
BstXI CCAn_nnnn'nTGG
Cuts at: 2240 2240
Size: 6649
BstYI r'GATC_y
Cuts at: 1972 2845 3364 3979 4346 4731 4748 6200 6286
Size: 873 519 615 367 385 17 1452 86
Cuts at: 6286 6298 6644 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 873 615 519 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2124 2757 2124
Size: 633 6016
BtgI C'CryG_G
Cuts at: 513 1065 2014 2184 3698 513
Size: 552 949 170 1514 3464
Fragments arranged by size:
3464 1514 949 552 170
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3293 487
Size: 683 56 2067 3843
Fragments arranged by size:
3843 2067 683 56
BtsI GCAGTG_nn'
Cuts at: 2388 2610 4165 4959 4979 2388
Size: 222 1555 794 20 4058
Fragments arranged by size:
4058 1555 794 222 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6649
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6614
DraI TTT'AAA
Cuts at: 3604 3953 3604
Size: 349 6300
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5656 809
Size: 507 4340 1802
Fragments arranged by size:
4340 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2507 4096
Size: 54 1425 50 275 18 357 320 1589
Cuts at: 4096 4985 6614 8
Size: 889 1629 43
Fragments arranged by size:
1629 1589 1425 889 357 320 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4096 1537
Size: 275 2284 4090
Fragments arranged by size:
4090 2284 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 3078 3411 3736 4579 1353
Size: 869 856 333 325 843 3423
Fragments arranged by size:
3423 869 856 843 333 325
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2114 5236 5610 5756 387
Size: 1064 138 525 3122 374 146 1280
Fragments arranged by size:
3122 1280 1064 525 374 146 138
EcoICRI GAG'CTC
Cuts at: 2024 2024
Size: 6649
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 3185 4712 5309 6096 6364 1239
Size: 393 544 1009 1527 597 787 268 1524
Fragments arranged by size:
1527 1524 1009 787 597 544 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2120 2172 2120
Size: 52 6597
EcoRI G'AATT_C
Cuts at: 3958 4336 3958
Size: 378 6271
EcoRV GAT'ATC
Cuts at: 3106 3106
Size: 6649
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2611 2251
Size: 360 6289
FspI TGC'GCA
Cuts at: 5155 5155
Size: 6649
HaeII r_GCGC'y
Cuts at: 1678 2084 5796 1678
Size: 406 3712 2531
Fragments arranged by size:
3712 2531 406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2121 2153 3497 3529 3629
Size: 32 135 32 549 32 1344 32 100
Cuts at: 3629 3645 3661 3677 3842 3874 5290 5322 5364
Size: 16 16 16 165 32 1416 32 42
Cuts at: 5364 5396 1373
Size: 32 2626
Fragments arranged by size:
2626 1416 1344 549 165 135 100 42
32 32 32 32 32 32 32 16
16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3993 4239 669
Size: 988 281 2055 246 3079
Fragments arranged by size:
3079 2055 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6649
HpaI GTT'AAC
Cuts at: 4239 4239
Size: 6649
KpnI G_GTAC'C
Cuts at: 3974 3974
Size: 6649
MfeI C'AATT_G
Cuts at: 4248 4248
Size: 6649
MluI A'CGCG_T
Cuts at: 6525 6525
Size: 6649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2443 2476 2630 3189 5763 5947 1305
Size: 1138 33 154 559 2574 184 2007
Fragments arranged by size:
2574 2007 1138 559 184 154 33
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4827
Fragments arranged by size:
4827 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2818 4607 4966 5125 518
Size: 648 1652 1789 359 159 2042
Fragments arranged by size:
2042 1789 1652 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2192 2212 4065 4742 5890 6135 1606
Size: 586 20 1853 677 1148 245 2120
Fragments arranged by size:
2120 1853 1148 677 586 245 20
NaeI GCC'GGC
Cuts at: 2097 2097
Size: 6649
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6097
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6649
NgoMIV G'CCGG_C
Cuts at: 2095 2095
Size: 6649
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6649
NotI GC'GGCC_GC
Cuts at: 4096 4096
Size: 6649
NspI r_CATG'y
Cuts at: 1132 1229 2962 3436 3680 4007 5552 1132
Size: 97 1733 474 244 327 1545 2229
Fragments arranged by size:
2229 1733 1545 474 327 244 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6649
PciI A'CATG_T
Cuts at: 3676 5548 3676
Size: 1872 4777
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6649
PfoI T'CCnGG_A
Cuts at: 3121 3121
Size: 6649
PmeI GTTT'AAAC
Cuts at: 3953 3953
Size: 6649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4718 4750 6231 6263 4718
Size: 32 1481 32 5104
Fragments arranged by size:
5104 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2120 2120
Size: 6649
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6649
PsiI TTA'TAA
Cuts at: 2601 4219 2601
Size: 1618 5031
PstI C_TGCA'G
Cuts at: 830 4001 830
Size: 3171 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6649
SacI G_AGCT'C
Cuts at: 2026 2026
Size: 6649
SalI G'TCGA_C
Cuts at: 1936 3991 1936
Size: 2055 4594
SapI GCTCTTCn'nnn_
Cuts at: 2222 3078 3736 2222
Size: 856 658 5135
Fragments arranged by size:
5135 856 658
SbfI CC_TGCA'GG
Cuts at: 4001 4001
Size: 6649
ScaI AGT'ACT
Cuts at: 4897 6609 4897
Size: 1712 4937
SfcI C'TryA_G
Cuts at: 826 1045 3446 3997 5132 5813 6004 826
Size: 219 2401 551 1135 681 191 1471
Fragments arranged by size:
2401 1471 1135 681 551 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6649
SmaI CCC'GGG
Cuts at: 1872 3976 1872
Size: 2104 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3924 4753 5654 5916 820
Size: 197 926 1981 829 901 262 1553
Fragments arranged by size:
1981 1553 926 901 829 262 197
SnaBI TAC'GTA
Cuts at: 493 3718 4440 493
Size: 3225 722 2702
Fragments arranged by size:
3225 2702 722
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6649
SphI G_CATG'C
Cuts at: 1132 4007 1132
Size: 2875 3774
SspI AAT'ATT
Cuts at: 5 52 3020 3706 3817 4573 5
Size: 47 2968 686 111 756 2081
Fragments arranged by size:
2968 2081 756 686 111 47
StuI AGG'CCT
Cuts at: 1517 2033 1517
Size: 516 6133
StyI C'CwwG_G
Cuts at: 513 1065 2028 4088 513
Size: 552 963 2060 3074
Fragments arranged by size:
3074 2060 963 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2243 4676 4693 4846 5031 5476 325
Size: 1255 663 2433 17 153 185 445 1498
Fragments arranged by size:
2433 1498 1255 663 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2288 4895 6607
Size: 274 80 33 51 1443 311 2607 1712
Cuts at: 6607 96
Size: 138
Fragments arranged by size:
2607 1712 1443 311 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3031 3063 3742 3774 6609 6641 3031
Size: 32 679 32 2835 32 3039
Fragments arranged by size:
3039 2835 679 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2071 3470 1316
Size: 755 1399 4495
Fragments arranged by size:
4495 1399 755
XbaI T'CTAG_A
Cuts at: 3647 3985 3647
Size: 338 6311
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2459 2517 2459
Size: 58 6591
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6649
XmaI C'CCGG_G
Cuts at: 1870 3974 1870
Size: 2104 4545
XmnI GAAnn'nnTTC
Cuts at: 4778 4778
Size: 6649
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4457 276
Size: 53 83 186 936 2923 2468
Fragments arranged by size:
2923 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoNI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI