Restriction map for
pFN21AB8685
(Circular) (Six-base) MAPSORT of: pFN21AB8685.seq Check: 4295 from: 1 to: 6856
pFN21AB8685 6856 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4666 278
Size: 53 83 186 936 3130 2468
Fragments arranged by size:
3130 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3609 4199 1937
Size: 1672 590 4594
Fragments arranged by size:
4594 1672 590
Acc65I G'GTAC_C
Cuts at: 4177 4177
Size: 6856
AclI AA'CG_TT
Cuts at: 1563 4983 5356 1563
Size: 3420 373 3063
Fragments arranged by size:
3420 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2499 2507 2883 4919 6303 6571 1632
Size: 867 8 376 2036 1384 268 1917
Fragments arranged by size:
2036 1917 1384 867 376 268 8
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6659
AflIII A'CryG_T
Cuts at: 1203 5755 6732 1203
Size: 4552 977 1327
Fragments arranged by size:
4552 1327 977
AgeI A'CCGG_T
Cuts at: 5687 5687
Size: 6856
AhdI GACnn_n'nnGTC
Cuts at: 2713 5585 2713
Size: 2872 3984
AleI CACnn'nnGTG
Cuts at: 1166 2559 3671 1166
Size: 1393 1112 4351
Fragments arranged by size:
4351 1393 1112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3611 1184
Size: 2427 4429
AlwNI CAG_nnn'CTG
Cuts at: 2110 2404 3849 4069 4599 6171 2110
Size: 294 1445 220 530 1572 2795
Fragments arranged by size:
2795 1572 1445 530 294 220
ApaI G_GGCC'C
Cuts at: 3454 3454
Size: 6856
ApaLI G'TGCA_C
Cuts at: 4913 6069 4913
Size: 1156 5700
ApoI r'AATT_y
Cuts at: 1493 3086 4165 4391 4543 6652 1493
Size: 1593 1079 226 152 2109 1697
Fragments arranged by size:
2109 1697 1593 1079 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5410 160
Size: 872 4378 1606
Fragments arranged by size:
4378 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6856
AvaI C'yCGr_G
Cuts at: 1870 1943 2123 2579 2792 3424 3893 3965 4181
Size: 73 180 456 213 632 469 72 216
Cuts at: 4181 1870
Size: 4545
Fragments arranged by size:
4545 632 469 456 216 213 180 73
72
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3992 4025 3992
Size: 33 6823
BamHI G'GATC_C
Cuts at: 4186 4553 4186
Size: 367 6489
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2483 2569 3269 3817 4177
Size: 325 217 627 696 86 700 548 360
Cuts at: 4177 5632 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 700 696 627 548 360 325
217 86
BanII G_rGCy'C
Cuts at: 1391 2346 2427 2580 2963 3454 4175 1391
Size: 955 81 153 383 491 721 4072
Fragments arranged by size:
4072 955 721 491 383 153 81
BbeI G_GCGC'C
Cuts at: 3273 3273
Size: 6856
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2218 3695 928
Size: 971 319 1477 4089
Fragments arranged by size:
4089 1477 971 319
BbvCI CC'TCA_GC
Cuts at: 2235 2304 2648 2235
Size: 69 344 6443
Fragments arranged by size:
6443 344 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5047 5081 1903
Size: 34 3110 34 3678
Fragments arranged by size:
3678 3110 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4748 5958 4748
Size: 1210 5646
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6856
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3267 3495 5467
Size: 107 122 71 631 605 1595 228 1972
Cuts at: 5467 136
Size: 1525
Fragments arranged by size:
1972 1595 1525 631 605 228 122 107
71
BglII A'GATC_T
Cuts at: 6851 6851
Size: 6856
BlpI GC'TnA_GC
Cuts at: 4273 4273
Size: 6856
Bme1580I G_kGCm'C
Cuts at: 1790 2488 2566 2910 3454 4917 6073 1790
Size: 698 78 344 544 1463 1156 2573
Fragments arranged by size:
2573 1463 1156 698 544 344 78
BmgBI CAC'GTC
Cuts at: 1318 3334 1318
Size: 2016 4840
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2423 3634 4008
Size: 416 53 83 399 24 1006 1211 374
Cuts at: 4008 4587 5545 442
Size: 579 958 1753
Fragments arranged by size:
1753 1211 1006 958 579 416 399 374
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6856
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2082 3280 2082
Size: 1198 5658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2130 2357 2609 2744 3324 3620 5516 1239
Size: 891 227 252 135 580 296 1896 2579
Fragments arranged by size:
2579 1896 891 580 296 252 227 135
Bpu10I CC'TnA_GC
Cuts at: 2028 2103 2235 2304 2574 2648 2957 3265 2028
Size: 75 132 69 270 74 309 308 5619
Fragments arranged by size:
5619 309 308 270 132 75 74 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4981 5846 6144 4981
Size: 865 298 5693
Fragments arranged by size:
5693 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2004 5519 882
Size: 617 505 3515 2219
Fragments arranged by size:
3515 2219 617 505
BsaAI yAC'GTr
Cuts at: 493 1204 4647 493
Size: 711 3443 2702
Fragments arranged by size:
3443 2702 711
BsaBI GATnn'nnATC
Cuts at: 6850 6850
Size: 6856
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2151 2949 3270 4663
Size: 53 83 186 936 618 798 321 1393
Cuts at: 4663 5045 5680 275
Size: 382 635 1451
Fragments arranged by size:
1451 1393 936 798 635 618 382 321
186 83 53
BsaWI w'CCGG_w
Cuts at: 5289 5687 5961 6108 6519 5289
Size: 398 274 147 411 5626
Fragments arranged by size:
5626 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6631
Fragments arranged by size:
6631 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2897 2900 3116 3119 3128 3131 3134
Size: 955 756 3 216 3 9 3 3
Cuts at: 3134 3143 3185 3188 3197 3206 3269 3530 3533
Size: 9 42 3 9 9 63 261 3
Cuts at: 3533 3545 3548 3581 3710 3713 3716 3731 3761
Size: 12 3 33 129 3 3 15 30
Cuts at: 3761 4073 4121 4124 4127 4130 4139 4142 4145
Size: 312 48 3 3 3 9 3 3
Cuts at: 4145 4148 4157 1186
Size: 3 9 3885
Fragments arranged by size:
3885 955 756 312 261 216 129 63
48 42 33 30 15 12 9 9
9 9 9 9 3 3 3 3
3 3 3 3 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2040 2154 2248 2487 2542 3493 3548 3945 6059
Size: 114 94 239 55 951 55 397 2114
Cuts at: 6059 6555 2040
Size: 496 2341
Fragments arranged by size:
2341 2114 951 496 397 239 114 94
55 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2621 3649 1163
Size: 573 885 1028 4370
Fragments arranged by size:
4370 1028 885 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3474 4306 5067 6095 1540
Size: 275 186 1473 832 761 1028 2301
Fragments arranged by size:
2301 1473 1028 832 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2346 2664 2963 4917 5002 6073 2346
Size: 318 299 1954 85 1071 3129
Fragments arranged by size:
3129 1954 1071 318 299 85
BsmI GAATG_Cn'
Cuts at: 4367 4460 4367
Size: 93 6763
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2346 2427 2488 2566 2580 2664 2910
Size: 399 556 81 61 78 14 84 246
Cuts at: 2910 2963 3454 4175 4917 5002 6073 1391
Size: 53 491 721 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 721 556 491 399 246
85 84 81 78 61 53 14
BspEI T'CCGG_A
Cuts at: 6519 6519
Size: 6856
BspHI T'CATG_A
Cuts at: 4745 6475 4745
Size: 1730 5126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2022 2323 2628 3453 3472 4211 844
Size: 1178 301 305 825 19 739 3489
Fragments arranged by size:
3489 1178 825 739 305 301 19
BsrBI CCG'CTC
Cuts at: 1394 1823 3264 4743 1394
Size: 429 1441 1479 3507
Fragments arranged by size:
3507 1479 1441 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5351 5525 65
Size: 1183 4103 174 1396
Fragments arranged by size:
4103 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2945 5500 5687 1700
Size: 110 1135 2555 187 2869
Fragments arranged by size:
2869 2555 1135 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6856
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6856
BssSI C'ACGA_G
Cuts at: 4916 5928 4916
Size: 1012 5844
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2640 3649 3849 794
Size: 1846 1009 200 3801
Fragments arranged by size:
3801 1846 1009 200
BstBI TT'CG_AA
Cuts at: 5660 5660
Size: 6856
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6856
BstXI CCAn_nnnn'nTGG
Cuts at: 2917 2917
Size: 6856
BstYI r'GATC_y
Cuts at: 1972 2685 3045 4186 4553 4938 4955 6407 6493
Size: 713 360 1141 367 385 17 1452 86
Cuts at: 6493 6505 6851 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1141 713 385 367 360 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2703 2703
Size: 6856
BtgI C'CryG_G
Cuts at: 513 1065 2004 3201 3314 3615 513
Size: 552 939 1197 113 301 3754
Fragments arranged by size:
3754 1197 939 552 301 113
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6117
Fragments arranged by size:
6117 683 56
BtsI GCAGTG_nn'
Cuts at: 2753 3773 4372 5166 5186 2753
Size: 1020 599 794 20 4423
Fragments arranged by size:
4423 1020 794 599 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6856
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6821
DraI TTT'AAA
Cuts at: 4160 4160
Size: 6856
DraIII CAC_nnn'GTG
Cuts at: 2748 2748
Size: 6856
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3401 5863 809
Size: 507 2085 2462 1802
Fragments arranged by size:
2462 2085 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2595 2988 3075
Size: 54 1425 50 275 18 765 393 87
Cuts at: 3075 3471 3618 3867 4303 5192 6821 8
Size: 396 147 249 436 889 1629 43
Fragments arranged by size:
1629 1425 889 765 436 396 393 275
249 147 87 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3471 4303 1537
Size: 275 1659 832 4090
Fragments arranged by size:
4090 1659 832 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3717 4050 4786 1353
Size: 2364 333 736 3423
Fragments arranged by size:
3423 2364 736 333
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2834 5443 5817 5963 387
Size: 1064 138 1245 2609 374 146 1280
Fragments arranged by size:
2609 1280 1245 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2344 2961 2344
Size: 617 6239
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 2357 2499 2507 2609 2744 2883
Size: 393 498 227 142 8 102 135 139
Cuts at: 2883 3324 3620 4919 5516 6303 6571 1239
Size: 441 296 1299 597 787 268 1524
Fragments arranged by size:
1524 1299 787 597 498 441 393 296
268 227 142 139 135 102 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2077 3653 3713 2077
Size: 1576 60 5220
Fragments arranged by size:
5220 1576 60
EcoO109I rG'GnC_Cy
Cuts at: 2107 2707 3706 3897 2107
Size: 600 999 191 5066
Fragments arranged by size:
5066 999 600 191
EcoRI G'AATT_C
Cuts at: 3086 4165 4543 3086
Size: 1079 378 5399
Fragments arranged by size:
5399 1079 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3394 3394
Size: 6856
FspI TGC'GCA
Cuts at: 5362 5362
Size: 6856
HaeII r_GCGC'y
Cuts at: 1678 2035 2837 3273 6003 1678
Size: 357 802 436 2730 2531
Fragments arranged by size:
2730 2531 802 436 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2252 2284 2459 2491 2699
Size: 32 135 32 680 32 175 32 208
Cuts at: 2699 2731 5497 5529 5571 5603 1373
Size: 32 2766 32 42 32 2626
Fragments arranged by size:
2766 2626 680 208 175 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2434 3610 4200 4446 669
Size: 988 281 496 1176 590 246 3079
Fragments arranged by size:
3079 1176 988 590 496 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6856
HpaI GTT'AAC
Cuts at: 4446 4446
Size: 6856
KasI G'GCGC_C
Cuts at: 3269 3269
Size: 6856
KpnI G_GTAC'C
Cuts at: 4181 4181
Size: 6856
MfeI C'AATT_G
Cuts at: 2638 4455 2638
Size: 1817 5039
MluI A'CGCG_T
Cuts at: 6732 6732
Size: 6856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3906 5970 6154 1305
Size: 2601 2064 184 2007
Fragments arranged by size:
2601 2064 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2990 3620 10
Size: 54 1425 343 1158 630 3246
Fragments arranged by size:
3246 1425 1158 630 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2559 3593 3671 4034 4814 5173 5332
Size: 648 1393 1034 78 363 780 359 159
Cuts at: 5332 518
Size: 2042
Fragments arranged by size:
2042 1393 1034 780 648 363 359 159
78
MspA1I CmG'CkG
Cuts at: 1606 2117 2368 2698 3001 3493 3663 4272 4949
Size: 511 251 330 303 492 170 609 677
Cuts at: 4949 6097 6342 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 609 511 492 330 303
251 245 170
NaeI GCC'GGC
Cuts at: 2947 2947
Size: 6856
NarI GG'CG_CC
Cuts at: 3270 3270
Size: 6856
NcoI C'CATG_G
Cuts at: 513 1065 2004 3314 3615 513
Size: 552 939 1310 301 3754
Fragments arranged by size:
3754 1310 939 552 301
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6856
NgoMIV G'CCGG_C
Cuts at: 2945 2945
Size: 6856
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6856
NotI GC'GGCC_GC
Cuts at: 4303 4303
Size: 6856
NspI r_CATG'y
Cuts at: 1132 1229 4214 5759 1132
Size: 97 2985 1545 2229
Fragments arranged by size:
2985 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4093 1348
Size: 2745 4111
PciI A'CATG_T
Cuts at: 5755 5755
Size: 6856
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2404 2748 3888 1108
Size: 1296 344 1140 4076
Fragments arranged by size:
4076 1296 1140 344
PfoI T'CCnGG_A
Cuts at: 2418 2721 3090 2418
Size: 303 369 6184
Fragments arranged by size:
6184 369 303
PmeI GTTT'AAAC
Cuts at: 4160 4160
Size: 6856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4925 4957 6438 6470 4925
Size: 32 1481 32 5311
Fragments arranged by size:
5311 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2707 3706 2707
Size: 999 5857
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6856
PsiI TTA'TAA
Cuts at: 4426 4426
Size: 6856
PspOMI G'GGCC_C
Cuts at: 3450 3450
Size: 6856
PstI C_TGCA'G
Cuts at: 830 2367 2628 3845 4208 830
Size: 1537 261 1217 363 3478
Fragments arranged by size:
3478 1537 1217 363 261
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6856
PvuII CAG'CTG
Cuts at: 3001 3001
Size: 6856
SacI G_AGCT'C
Cuts at: 2346 2963 2346
Size: 617 6239
SalI G'TCGA_C
Cuts at: 1936 3608 4198 1936
Size: 1672 590 4594
Fragments arranged by size:
4594 1672 590
SbfI CC_TGCA'GG
Cuts at: 4208 4208
Size: 6856
ScaI AGT'ACT
Cuts at: 3860 5104 6816 3860
Size: 1244 1712 3900
Fragments arranged by size:
3900 1712 1244
SexAI A'CCwGG_T
Cuts at: 2664 3069 2664
Size: 405 6451
SfcI C'TryA_G
Cuts at: 826 1045 2363 2557 2624 3841 4204 5339 6020
Size: 219 1318 194 67 1217 363 1135 681
Cuts at: 6020 6211 826
Size: 191 1471
Fragments arranged by size:
1471 1318 1217 1135 681 363 219 194
191 67
SfoI GGC'GCC
Cuts at: 3271 3271
Size: 6856
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6856
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6856
SmaI CCC'GGG
Cuts at: 1872 3426 3895 4183 1872
Size: 1554 469 288 4545
Fragments arranged by size:
4545 1554 469 288
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4960 5861 6123 820
Size: 197 926 3017 901 262 1553
Fragments arranged by size:
3017 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4647 493
Size: 4154 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6856
SphI G_CATG'C
Cuts at: 1132 4214 1132
Size: 3082 3774
SspI AAT'ATT
Cuts at: 5 52 4780 5
Size: 47 4728 2081
Fragments arranged by size:
4728 2081 47
StuI AGG'CCT
Cuts at: 1517 2072 2615 1517
Size: 555 543 5758
Fragments arranged by size:
5758 555 543
StyI C'CwwG_G
Cuts at: 513 1065 2004 2439 2610 3249 3314 3615 4295
Size: 552 939 435 171 639 65 301 680
Cuts at: 4295 513
Size: 3074
Fragments arranged by size:
3074 939 680 639 552 435 301 171
65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2240 3616 3661 4883 4900 5053 5238
Size: 1255 660 1376 45 1222 17 153 185
Cuts at: 5238 5683 325
Size: 445 1498
Fragments arranged by size:
1498 1376 1255 1222 660 445 185 153
45 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3285 3858 3957
Size: 274 80 33 51 1443 1308 573 99
Cuts at: 3957 5102 6814 96
Size: 1145 1712 138
Fragments arranged by size:
1712 1443 1308 1145 573 274 138 99
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2269 2301 3630 3662 6816 6848 2269
Size: 32 1329 32 3154 32 2277
Fragments arranged by size:
3154 2277 1329 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3209 1316
Size: 1893 4963
XbaI T'CTAG_A
Cuts at: 4192 4192
Size: 6856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2812 2998 2812
Size: 186 6670
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6856
XmaI C'CCGG_G
Cuts at: 1870 3424 3893 4181 1870
Size: 1554 469 288 4545
Fragments arranged by size:
4545 1554 469 288
XmnI GAAnn'nnTTC
Cuts at: 3377 4985 3377
Size: 1608 5248
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4664 276
Size: 53 83 186 936 3130 2468
Fragments arranged by size:
3130 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BfrBI BsiWI BsmBI
BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI
SwaI