Restriction map for
pFN21AB8683
(Circular) (Six-base) MAPSORT of: pFN21AB8683.seq Check: 5095 from: 1 to: 6721
pFN21AB8683 6721 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4531 278
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4064 1937
Size: 2127 4594
Acc65I G'GTAC_C
Cuts at: 2938 4042 2938
Size: 1104 5617
AclI AA'CG_TT
Cuts at: 1563 4848 5221 1563
Size: 3285 373 3063
Fragments arranged by size:
3285 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2095 2114 2198 2225 2870 2895 2927 4784
Size: 463 19 84 27 645 25 32 1857
Cuts at: 4784 6168 6436 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1857 1384 645 463 268 84 32
27 25 19
AflII C'TTAA_G
Cuts at: 820 1017 2446 820
Size: 197 1429 5095
Fragments arranged by size:
5095 1429 197
AflIII A'CryG_T
Cuts at: 1203 3147 5620 6597 1203
Size: 1944 2473 977 1327
Fragments arranged by size:
2473 1944 1327 977
AgeI A'CCGG_T
Cuts at: 5552 5552
Size: 6721
AhdI GACnn_n'nnGTC
Cuts at: 3364 5450 3364
Size: 2086 4635
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3333 1184
Size: 2149 4572
AlwNI CAG_nnn'CTG
Cuts at: 2575 4464 6036 2575
Size: 1889 1572 3260
Fragments arranged by size:
3260 1889 1572
ApaLI G'TGCA_C
Cuts at: 4778 5934 4778
Size: 1156 5565
ApoI r'AATT_y
Cuts at: 1493 2087 2348 3128 3834 3881 4030 4256 4408
Size: 594 261 780 706 47 149 226 152
Cuts at: 4408 6517 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 780 706 594 261 226 152
149 47
AseI AT'TA_AT
Cuts at: 160 1032 5275 160
Size: 872 4243 1606
Fragments arranged by size:
4243 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
AvaI C'yCGr_G
Cuts at: 1870 1943 3599 4046 1870
Size: 73 1656 447 4545
Fragments arranged by size:
4545 1656 447 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3454 3487 3454
Size: 33 6688
BamHI G'GATC_C
Cuts at: 4051 4418 4051
Size: 367 6354
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2642 2938 4042 5497 618
Size: 325 217 627 855 296 1104 1455 1842
Fragments arranged by size:
1842 1455 1104 855 627 325 296 217
BanII G_rGCy'C
Cuts at: 1391 2529 2586 3091 3600 3689 4040 1391
Size: 1138 57 505 509 89 351 4072
Fragments arranged by size:
4072 1138 509 505 351 89 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2564 2634 2876 3197 928
Size: 971 665 70 242 321 4452
Fragments arranged by size:
4452 971 665 321 242 70
BbvCI CC'TCA_GC
Cuts at: 2214 2333 2487 2214
Size: 119 154 6448
Fragments arranged by size:
6448 154 119
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4912 4946 1903
Size: 34 2975 34 3678
Fragments arranged by size:
3678 2975 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2695 4613 5823 2695
Size: 1918 1210 3593
Fragments arranged by size:
3593 1918 1210
BclI T'GATC_A
Cuts at: 1548 2634 1548
Size: 1086 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3248 5332 136
Size: 107 122 71 631 605 1576 2084 1525
Fragments arranged by size:
2084 1576 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6716 6716
Size: 6721
BlpI GC'TnA_GC
Cuts at: 4138 4138
Size: 6721
Bme1580I G_kGCm'C
Cuts at: 1790 4782 5938 1790
Size: 2992 1156 2573
Fragments arranged by size:
2992 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6721
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3959 4452 5410
Size: 416 53 83 399 24 2542 493 958
Cuts at: 5410 442
Size: 1753
Fragments arranged by size:
2542 1753 958 493 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2441 2740 2858 3065 3206 3416 5381
Size: 971 231 299 118 207 141 210 1965
Cuts at: 5381 1239
Size: 2579
Fragments arranged by size:
2579 1965 971 299 231 210 207 141
118
Bpu10I CC'TnA_GC
Cuts at: 2214 2333 2487 2214
Size: 119 154 6448
Fragments arranged by size:
6448 154 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2531 2813 4846 5711 6009 2531
Size: 282 2033 865 298 3243
Fragments arranged by size:
3243 2033 865 298 282
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2018 5384 882
Size: 617 519 3366 2219
Fragments arranged by size:
3366 2219 617 519
BsaAI yAC'GTr
Cuts at: 493 1204 2590 3150 3502 4512 493
Size: 711 1386 560 352 1010 2702
Fragments arranged by size:
2702 1386 1010 711 560 352
BsaBI GATnn'nnATC
Cuts at: 6715 6715
Size: 6721
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4528 4910 5545 275
Size: 53 83 186 936 2995 382 635 1451
Fragments arranged by size:
2995 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2757 5154 5552 5826 5973 6384 2757
Size: 2397 398 274 147 411 3094
Fragments arranged by size:
3094 2397 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6496
Fragments arranged by size:
6496 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3302 3595 1186
Size: 2116 293 4312
Fragments arranged by size:
4312 2116 293
BseYI C'CCAG_C
Cuts at: 2236 2475 3001 3273 3688 5924 6420 2236
Size: 239 526 272 415 2236 496 2537
Fragments arranged by size:
2537 2236 526 496 415 272 239
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6148
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3217 4171 4932 5960 1540
Size: 275 186 1216 954 761 1028 2301
Fragments arranged by size:
2301 1216 1028 954 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2586 4782 4867 5938 2586
Size: 2196 85 1071 3369
Fragments arranged by size:
3369 2196 1071 85
BsmI GAATG_Cn'
Cuts at: 2361 2992 3322 4232 4325 2361
Size: 631 330 910 93 4757
Fragments arranged by size:
4757 910 631 330 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2529 2586 3091 3600 3689 4040 4782
Size: 399 739 57 505 509 89 351 742
Cuts at: 4782 4867 5938 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 739 509 505 399 351
89 85 57
BspEI T'CCGG_A
Cuts at: 6384 6384
Size: 6721
BspHI T'CATG_A
Cuts at: 2679 4610 6340 2679
Size: 1931 1730 3060
Fragments arranged by size:
3060 1931 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4076 844
Size: 3232 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2068 2752 4608 1394
Size: 429 245 684 1856 3507
Fragments arranged by size:
3507 1856 684 429 245
BsrDI GCAATG_nn'
Cuts at: 65 1248 5216 5390 65
Size: 1183 3968 174 1396
Fragments arranged by size:
3968 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5365 5552 1700
Size: 110 3555 187 2869
Fragments arranged by size:
3555 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2277 96
Size: 2181 4540
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6721
BssSI C'ACGA_G
Cuts at: 3206 4781 5793 3206
Size: 1575 1012 4134
Fragments arranged by size:
4134 1575 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6721
BstBI TT'CG_AA
Cuts at: 5525 5525
Size: 6721
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6721
BstXI CCAn_nnnn'nTGG
Cuts at: 2419 3260 3328 3502 2419
Size: 841 68 174 5638
Fragments arranged by size:
5638 841 174 68
BstYI r'GATC_y
Cuts at: 1972 2198 3162 4051 4418 4803 4820 6272 6358
Size: 226 964 889 367 385 17 1452 86
Cuts at: 6358 6370 6716 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 964 889 385 367 346 226
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2283 513
Size: 552 1218 4951
Fragments arranged by size:
4951 1218 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5982
Fragments arranged by size:
5982 683 56
BtsI GCAGTG_nn'
Cuts at: 2875 3284 3623 4237 5031 5051 2875
Size: 409 339 614 794 20 4545
Fragments arranged by size:
4545 794 614 409 339 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6721
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6686
DraI TTT'AAA
Cuts at: 4025 4025
Size: 6721
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5728 809
Size: 507 4412 1802
Fragments arranged by size:
4412 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 3214 4168
Size: 54 1425 50 275 18 225 1159 954
Cuts at: 4168 5057 6686 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1159 954 889 275 225 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3214 4168 1537
Size: 275 1402 954 4090
Fragments arranged by size:
4090 1402 954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2928 3814 3928 4651 1353
Size: 1575 886 114 723 3423
Fragments arranged by size:
3423 1575 886 723 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2555 5308 5682 5828 387
Size: 1064 138 966 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 966 374 146 138
EcoICRI GAG'CTC
Cuts at: 2584 2584
Size: 6721
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2114 2198 2210 2225 2441 2740
Size: 393 463 19 84 12 15 216 299
Cuts at: 2740 2858 2870 2895 2927 3065 3206 3416 4784
Size: 118 12 25 32 138 141 210 1368
Cuts at: 4784 5381 6168 6436 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1368 787 597 463 393 299 268
216 210 141 138 118 84 32 25
19 15 12 12
EcoO109I rG'GnC_Cy
Cuts at: 2434 2671 3040 3391 3754 2434
Size: 237 369 351 363 5401
Fragments arranged by size:
5401 369 363 351 237
EcoRI G'AATT_C
Cuts at: 2348 3881 4030 4408 2348
Size: 1533 149 378 4661
Fragments arranged by size:
4661 1533 378 149
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2424 2900 2424
Size: 476 6245
FspI TGC'GCA
Cuts at: 5227 5227
Size: 6721
HaeII r_GCGC'y
Cuts at: 1678 5868 1678
Size: 4190 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2326 2358 3065 3097 3350
Size: 32 135 32 754 32 707 32 253
Cuts at: 3350 3382 5362 5394 5436 5468 1373
Size: 32 1980 32 42 32 2626
Fragments arranged by size:
2626 1980 754 707 253 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4065 4311 669
Size: 988 281 2127 246 3079
Fragments arranged by size:
3079 2127 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3407 3534 748
Size: 2659 127 3935
Fragments arranged by size:
3935 2659 127
HpaI GTT'AAC
Cuts at: 4311 4311
Size: 6721
KpnI G_GTAC'C
Cuts at: 2942 4046 2942
Size: 1104 5617
MfeI C'AATT_G
Cuts at: 4320 4320
Size: 6721
MluI A'CGCG_T
Cuts at: 6597 6597
Size: 6721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 2941 5835 6019 1305
Size: 684 952 2894 184 2007
Fragments arranged by size:
2894 2007 952 684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4899
Fragments arranged by size:
4899 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2849 4679 5038 5197 518
Size: 648 1683 1830 359 159 2042
Fragments arranged by size:
2042 1830 1683 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3219 4137 4814 5962 6207 1606
Size: 1613 918 677 1148 245 2120
Fragments arranged by size:
2120 1613 1148 918 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2283 513
Size: 552 1218 4951
Fragments arranged by size:
4951 1218 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6721
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6721
NotI GC'GGCC_GC
Cuts at: 4168 4168
Size: 6721
NspI r_CATG'y
Cuts at: 1132 1229 4079 5624 1132
Size: 97 2850 1545 2229
Fragments arranged by size:
2850 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6721
PciI A'CATG_T
Cuts at: 5620 5620
Size: 6721
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2575 1108
Size: 1467 5254
PmeI GTTT'AAAC
Cuts at: 4025 4025
Size: 6721
PmlI CAC'GTG
Cuts at: 3150 3150
Size: 6721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4790 4822 6303 6335 4790
Size: 32 1481 32 5176
Fragments arranged by size:
5176 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2434 2671 3040 2434
Size: 237 369 6115
Fragments arranged by size:
6115 369 237
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6721
PsiI TTA'TAA
Cuts at: 4291 4291
Size: 6721
PstI C_TGCA'G
Cuts at: 830 3027 3684 4073 830
Size: 2197 657 389 3478
Fragments arranged by size:
3478 2197 657 389
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6721
SacI G_AGCT'C
Cuts at: 2586 2586
Size: 6721
SalI G'TCGA_C
Cuts at: 1936 4063 1936
Size: 2127 4594
SbfI CC_TGCA'GG
Cuts at: 4073 4073
Size: 6721
ScaI AGT'ACT
Cuts at: 4969 6681 4969
Size: 1712 5009
SfcI C'TryA_G
Cuts at: 826 1045 3023 3680 4069 5204 5885 6076 826
Size: 219 1978 657 389 1135 681 191 1471
Fragments arranged by size:
1978 1471 1135 681 657 389 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3248 3248
Size: 6721
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6721
SmaI CCC'GGG
Cuts at: 1872 4048 1872
Size: 2176 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2446 2546 2828 4825 5726 5988
Size: 197 926 503 100 282 1997 901 262
Cuts at: 5988 820
Size: 1553
Fragments arranged by size:
1997 1553 926 901 503 282 262 197
100
SnaBI TAC'GTA
Cuts at: 493 4512 493
Size: 4019 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6721
SphI G_CATG'C
Cuts at: 1132 4079 1132
Size: 2947 3774
SspI AAT'ATT
Cuts at: 5 52 4645 5
Size: 47 4593 2081
Fragments arranged by size:
4593 2081 47
StuI AGG'CCT
Cuts at: 1517 2338 2540 3242 3847 1517
Size: 821 202 702 605 4391
Fragments arranged by size:
4391 821 702 605 202
StyI C'CwwG_G
Cuts at: 513 1065 2283 2304 2517 3054 3450 3750 4160
Size: 552 1218 21 213 537 396 300 410
Cuts at: 4160 513
Size: 3074
Fragments arranged by size:
3074 1218 552 537 410 396 300 213
21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4748 4765 4918 5103 5548 325
Size: 1255 3168 17 153 185 445 1498
Fragments arranged by size:
3168 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 4967 6679
Size: 274 80 33 51 1443 300 2690 1712
Cuts at: 6679 96
Size: 138
Fragments arranged by size:
2690 1712 1443 300 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3290 3322 6681 6713 3290
Size: 32 3359 32 3298
Fragments arranged by size:
3359 3298 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6721
XbaI T'CTAG_A
Cuts at: 4057 4057
Size: 6721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3328 3733 3328
Size: 405 6316
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6721
XmaI C'CCGG_G
Cuts at: 1870 4046 1870
Size: 2176 4545
XmnI GAAnn'nnTTC
Cuts at: 2404 4850 2404
Size: 2446 4275
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4529 276
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfoI SrfI SwaI