Restriction map for
pFN21AB8679
(Circular) (Six-base) MAPSORT of: pFN21AB8679.seq Check: 1761 from: 1 to: 6247
pFN21AB8679 6247 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3238 3238
Size: 6247
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4057 278
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3590 1937
Size: 1653 4594
Acc65I G'GTAC_C
Cuts at: 3568 3568
Size: 6247
AclI AA'CG_TT
Cuts at: 1563 4374 4747 1563
Size: 2811 373 3063
Fragments arranged by size:
3063 2811 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 2261 3312 4310 5694 5962 1632
Size: 492 137 1051 998 1384 268 1917
Fragments arranged by size:
1917 1384 1051 998 492 268 137
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6050
AflIII A'CryG_T
Cuts at: 1203 2148 5146 6123 1203
Size: 945 2998 977 1327
Fragments arranged by size:
2998 1327 977 945
AgeI A'CCGG_T
Cuts at: 5078 5078
Size: 6247
AhdI GACnn_n'nnGTC
Cuts at: 4976 4976
Size: 6247
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3333 1184
Size: 2149 4098
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2737 2769 2737
Size: 32 6215
AlwNI CAG_nnn'CTG
Cuts at: 2818 3990 5562 2818
Size: 1172 1572 3503
Fragments arranged by size:
3503 1572 1172
ApaI G_GGCC'C
Cuts at: 2521 2521
Size: 6247
ApaLI G'TGCA_C
Cuts at: 2696 4304 5460 2696
Size: 1608 1156 3483
Fragments arranged by size:
3483 1608 1156
ApoI r'AATT_y
Cuts at: 1493 2108 2176 3556 3782 3934 6043 1493
Size: 615 68 1380 226 152 2109 1697
Fragments arranged by size:
2109 1697 1380 615 226 152 68
AseI AT'TA_AT
Cuts at: 160 1032 2280 3050 4801 160
Size: 872 1248 770 1751 1606
Fragments arranged by size:
1751 1606 1248 872 770
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
AvaI C'yCGr_G
Cuts at: 1870 1943 3572 1870
Size: 73 1629 4545
Fragments arranged by size:
4545 1629 73
BamHI G'GATC_C
Cuts at: 2377 3577 3944 2377
Size: 1200 367 4680
Fragments arranged by size:
4680 1200 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2537 2672 3568 5023 618
Size: 325 217 627 750 135 896 1455 1842
Fragments arranged by size:
1842 1455 896 750 627 325 217 135
BanII G_rGCy'C
Cuts at: 1391 2521 3566 1391
Size: 1130 1045 4072
Fragments arranged by size:
4072 1130 1045
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2360 928
Size: 971 461 4815
Fragments arranged by size:
4815 971 461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2723 2757 4438 4472 1903
Size: 34 786 34 1681 34 3678
Fragments arranged by size:
3678 1681 786 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2331 4139 5349 2331
Size: 1808 1210 3229
Fragments arranged by size:
3229 1808 1210
BclI T'GATC_A
Cuts at: 1548 3111 3180 3399 1548
Size: 1563 69 219 4396
Fragments arranged by size:
4396 1563 219 69
BfrBI ATG'CAT
Cuts at: 2794 2794
Size: 6247
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4858 136
Size: 107 122 71 631 605 3186 1525
Fragments arranged by size:
3186 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6242 6242
Size: 6247
BlpI GC'TnA_GC
Cuts at: 3664 3664
Size: 6247
Bme1580I G_kGCm'C
Cuts at: 1790 2364 2521 2700 4308 5464 1790
Size: 574 157 179 1608 1156 2573
Fragments arranged by size:
2573 1608 1156 574 179 157
BmgBI CAC'GTC
Cuts at: 1318 2151 1318
Size: 833 5414
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3978 4936 442
Size: 416 53 83 399 24 2561 958 1753
Fragments arranged by size:
2561 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2778 3366 3473 3492 4907 1239
Size: 1539 588 107 19 1415 2579
Fragments arranged by size:
2579 1539 1415 588 107 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4372 5237 5535 4372
Size: 865 298 5084
Fragments arranged by size:
5084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4910 882
Size: 617 3411 2219
Fragments arranged by size:
3411 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4038 493
Size: 711 2834 2702
Fragments arranged by size:
2834 2702 711
BsaBI GATnn'nnATC
Cuts at: 6241 6241
Size: 6247
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4054 4436 5071 275
Size: 53 83 186 936 2521 382 635 1451
Fragments arranged by size:
2521 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4680 5078 5352 5499 5910 4680
Size: 398 274 147 411 5017
Fragments arranged by size:
5017 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2048 2078 2737 2767 1178
Size: 30 165 30 645 30 659 30 4658
Fragments arranged by size:
4658 659 645 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3369 3381 1186
Size: 2183 12 4052
Fragments arranged by size:
4052 2183 12
BseYI C'CCAG_C
Cuts at: 2505 5450 5946 2505
Size: 2945 496 2806
Fragments arranged by size:
2945 2806 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2465 2469 2937 1163
Size: 573 729 4 468 4473
Fragments arranged by size:
4473 729 573 468 4
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3697 4458 5486 1540
Size: 275 186 1696 761 1028 2301
Fragments arranged by size:
2301 1696 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2700 4308 4393 5464 2700
Size: 1608 85 1071 3483
Fragments arranged by size:
3483 1608 1071 85
BsmI GAATG_Cn'
Cuts at: 3758 3851 3758
Size: 93 6154
BsmBI CGTCTCn'nnnn_
Cuts at: 2157 2157
Size: 6247
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2364 2521 2700 3566 4308 4393 5464
Size: 399 574 157 179 866 742 85 1071
Cuts at: 5464 1391
Size: 2174
Fragments arranged by size:
2174 1071 866 742 574 399 179 157
85
BspEI T'CCGG_A
Cuts at: 5910 5910
Size: 6247
BspHI T'CATG_A
Cuts at: 2763 4136 5866 2763
Size: 1373 1730 3144
Fragments arranged by size:
3144 1730 1373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2856 3083 3238 3301 3602 844
Size: 2012 227 155 63 301 3489
Fragments arranged by size:
3489 2012 301 227 155 63
BsrBI CCG'CTC
Cuts at: 1394 1823 2059 3459 4134 1394
Size: 429 236 1400 675 3507
Fragments arranged by size:
3507 1400 675 429 236
BsrDI GCAATG_nn'
Cuts at: 65 1248 2066 4742 4916 65
Size: 1183 818 2676 174 1396
Fragments arranged by size:
2676 1396 1183 818 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4891 5078 1700
Size: 110 3081 187 2869
Fragments arranged by size:
3081 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6247
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6247
BssSI C'ACGA_G
Cuts at: 4307 5319 4307
Size: 1012 5235
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2800 2924 3335 794
Size: 2006 124 411 3706
Fragments arranged by size:
3706 2006 411 124
BstBI TT'CG_AA
Cuts at: 5051 5051
Size: 6247
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6247
BstXI CCAn_nnnn'nTGG
Cuts at: 2428 2428
Size: 6247
BstYI r'GATC_y
Cuts at: 1972 2377 3577 3944 4329 4346 5798 5884 5896
Size: 405 1200 367 385 17 1452 86 12
Cuts at: 5896 6242 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1200 405 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3082 513
Size: 552 939 1078 3678
Fragments arranged by size:
3678 1078 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5508
Fragments arranged by size:
5508 683 56
BtsI GCAGTG_nn'
Cuts at: 3763 4557 4577 3763
Size: 794 20 5433
Fragments arranged by size:
5433 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6247
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6212
DraI TTT'AAA
Cuts at: 3551 3551
Size: 6247
DraIII CAC_nnn'GTG
Cuts at: 2785 2785
Size: 6247
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5254 809
Size: 507 3938 1802
Fragments arranged by size:
3938 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3694 4583 6212
Size: 54 1425 50 275 18 1864 889 1629
Cuts at: 6212 8
Size: 43
Fragments arranged by size:
1864 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3694 1537
Size: 275 1882 4090
Fragments arranged by size:
4090 1882 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2237 2311 3413 4177 1353
Size: 884 74 1102 764 3423
Fragments arranged by size:
3423 1102 884 764 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4834 5208 5354 387
Size: 1064 138 435 2810 374 146 1280
Fragments arranged by size:
2810 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2261 2778 3312 3366 3473 3492
Size: 393 492 137 517 534 54 107 19
Cuts at: 3492 4310 4907 5694 5962 1239
Size: 818 597 787 268 1524
Fragments arranged by size:
1524 818 787 597 534 517 492 393
268 137 107 54 19
EcoO109I rG'GnC_Cy
Cuts at: 2502 2517 2518 3190 2502
Size: 15 1 672 5559
Fragments arranged by size:
5559 672 15 1
EcoRI G'AATT_C
Cuts at: 3556 3934 3556
Size: 378 5869
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2129 2129
Size: 6247
FspI TGC'GCA
Cuts at: 4753 4753
Size: 6247
HaeII r_GCGC'y
Cuts at: 1678 5394 1678
Size: 3716 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 2279 2311 2631
Size: 32 135 32 476 32 199 32 320
Cuts at: 2631 2663 3015 3047 4888 4920 4962 4994 1373
Size: 32 352 32 1841 32 42 32 2626
Fragments arranged by size:
2626 1841 476 352 320 199 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2883 3256 3591 3837 669
Size: 988 281 945 373 335 246 3079
Fragments arranged by size:
3079 988 945 373 335 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6247
HpaI GTT'AAC
Cuts at: 3837 3837
Size: 6247
KpnI G_GTAC'C
Cuts at: 3572 3572
Size: 6247
MfeI C'AATT_G
Cuts at: 3846 3846
Size: 6247
MluI A'CGCG_T
Cuts at: 6123 6123
Size: 6247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2124 5361 5545 1305
Size: 819 3237 184 2007
Fragments arranged by size:
3237 2007 819 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4425
Fragments arranged by size:
4425 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2835 4205 4564 4723 518
Size: 648 1669 1370 359 159 2042
Fragments arranged by size:
2042 1669 1370 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3062 3663 4340 5488 5733 1606
Size: 1456 601 677 1148 245 2120
Fragments arranged by size:
2120 1456 1148 677 601 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3082 513
Size: 552 939 1078 3678
Fragments arranged by size:
3678 1078 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6247
NotI GC'GGCC_GC
Cuts at: 3694 3694
Size: 6247
NsiI A_TGCA'T
Cuts at: 2796 2796
Size: 6247
NspI r_CATG'y
Cuts at: 1132 1229 2226 3605 5150 1132
Size: 97 997 1379 1545 2229
Fragments arranged by size:
2229 1545 1379 997 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6247
PciI A'CATG_T
Cuts at: 5146 5146
Size: 6247
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2317 1108
Size: 1209 5038
PmeI GTTT'AAAC
Cuts at: 3551 3551
Size: 6247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2769 4316 4348 5829 5861 2737
Size: 32 1547 32 1481 32 3123
Fragments arranged by size:
3123 1547 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2502 2502
Size: 6247
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6247
PsiI TTA'TAA
Cuts at: 3817 3817
Size: 6247
PspOMI G'GGCC_C
Cuts at: 2517 2517
Size: 6247
PstI C_TGCA'G
Cuts at: 830 2572 3599 830
Size: 1742 1027 3478
Fragments arranged by size:
3478 1742 1027
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6247
SalI G'TCGA_C
Cuts at: 1936 3589 1936
Size: 1653 4594
SanDI GG'GwC_CC
Cuts at: 2502 2502
Size: 6247
SbfI CC_TGCA'GG
Cuts at: 3599 3599
Size: 6247
ScaI AGT'ACT
Cuts at: 4495 6207 4495
Size: 1712 4535
SfcI C'TryA_G
Cuts at: 826 1045 2568 3259 3595 4730 5411 5602 826
Size: 219 1523 691 336 1135 681 191 1471
Fragments arranged by size:
1523 1471 1135 691 681 336 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6247
SmaI CCC'GGG
Cuts at: 1872 3574 1872
Size: 1702 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4351 5252 5514 820
Size: 197 926 2408 901 262 1553
Fragments arranged by size:
2408 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4038 493
Size: 3545 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6247
SphI G_CATG'C
Cuts at: 1132 3605 1132
Size: 2473 3774
SspI AAT'ATT
Cuts at: 5 52 4171 5
Size: 47 4119 2081
Fragments arranged by size:
4119 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6247
StyI C'CwwG_G
Cuts at: 513 1065 2004 2382 2701 3082 3686 513
Size: 552 939 378 319 381 604 3074
Fragments arranged by size:
3074 939 604 552 381 378 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2644 4274 4291 4444 4629 5074 325
Size: 1255 1064 1630 17 153 185 445 1498
Fragments arranged by size:
1630 1498 1255 1064 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4493 6205 96
Size: 274 80 33 51 1443 2516 1712 138
Fragments arranged by size:
2516 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2677 2709 6207 6239 2677
Size: 32 3498 32 2685
Fragments arranged by size:
3498 2685 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6247
XbaI T'CTAG_A
Cuts at: 3583 3583
Size: 6247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3280 3448 3280
Size: 168 6079
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6247
XmaI C'CCGG_G
Cuts at: 1870 3572 1870
Size: 1702 4545
XmnI GAAnn'nnTTC
Cuts at: 2109 4376 2109
Size: 2267 3980
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4055 276
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I
BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspXI PsrI PvuII RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI