Restriction map for
pFN21AB8676
(Circular) (Six-base) MAPSORT of: pFN21AB8676.seq Check: 4305 from: 1 to: 5971
pFN21AB8676 5971 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2314 2314
Size: 5971
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 1937
Size: 1377 4594
Acc65I G'GTAC_C
Cuts at: 3202 3292 3202
Size: 90 5881
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2367 3009 4034 5418 5686 1632
Size: 735 642 1025 1384 268 1917
Fragments arranged by size:
1917 1384 1025 735 642 268
AflII C'TTAA_G
Cuts at: 820 1017 2264 820
Size: 197 1247 4527
Fragments arranged by size:
4527 1247 197
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5971
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2883 2915 2883
Size: 32 5939
AlwNI CAG_nnn'CTG
Cuts at: 3054 3714 5286 3054
Size: 660 1572 3739
Fragments arranged by size:
3739 1572 660
ApaI G_GGCC'C
Cuts at: 2385 2385
Size: 5971
ApaLI G'TGCA_C
Cuts at: 4028 5184 4028
Size: 1156 4815
ApoI r'AATT_y
Cuts at: 1493 2723 3280 3506 3658 5767 1493
Size: 1230 557 226 152 2109 1697
Fragments arranged by size:
2109 1697 1230 557 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 2838 3296 1870
Size: 73 895 458 4545
Fragments arranged by size:
4545 895 458 73
AvrII C'CTAG_G
Cuts at: 2213 2213
Size: 5971
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2966 2999 2966
Size: 33 5938
BamHI G'GATC_C
Cuts at: 2943 3301 3668 2943
Size: 358 367 5246
Fragments arranged by size:
5246 367 358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2326 3202 3292 4747 618
Size: 325 217 627 539 876 90 1455 1842
Fragments arranged by size:
1842 1455 876 627 539 325 217 90
BanII G_rGCy'C
Cuts at: 1391 2385 2469 3290 1391
Size: 994 84 821 4072
Fragments arranged by size:
4072 994 821 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2651 928
Size: 971 752 4248
Fragments arranged by size:
4248 971 752
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4162 4196 1903
Size: 34 2225 34 3678
Fragments arranged by size:
3678 2225 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2994 3863 5073 2994
Size: 869 1210 3892
Fragments arranged by size:
3892 1210 869
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4582 136
Size: 107 122 71 631 605 2910 1525
Fragments arranged by size:
2910 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 2316 2799 3388 2316
Size: 483 589 4899
Fragments arranged by size:
4899 589 483
Bme1580I G_kGCm'C
Cuts at: 1790 2385 4032 5188 1790
Size: 595 1647 1156 2573
Fragments arranged by size:
2573 1647 1156 595
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5971
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2418 3042 3702
Size: 416 53 83 399 24 1001 624 660
Cuts at: 3702 4660 442
Size: 958 1753
Fragments arranged by size:
1753 1001 958 660 624 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2916 1056
Size: 1860 4111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2375 4631 1239
Size: 1136 2256 2579
Fragments arranged by size:
2579 2256 1136
Bpu10I CC'TnA_GC
Cuts at: 2222 2222
Size: 5971
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2172 2426 2858 4096 4961 5259 2172
Size: 254 432 1238 865 298 2884
Fragments arranged by size:
2884 1238 865 432 298 254
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2899 3037 4634 882
Size: 617 1400 138 1597 2219
Fragments arranged by size:
2219 1597 1400 617 138
BsaAI yAC'GTr
Cuts at: 493 1204 3762 493
Size: 711 2558 2702
Fragments arranged by size:
2702 2558 711
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3778 4160 4795 275
Size: 53 83 186 936 2245 382 635 1451
Fragments arranged by size:
2245 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4404 4802 5076 5223 5634 4404
Size: 398 274 147 411 4741
Fragments arranged by size:
4741 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2007 2037 2057 2087 2235
Size: 30 165 30 604 30 20 30 148
Cuts at: 2235 2265 2883 2913 1178
Size: 30 618 30 4236
Fragments arranged by size:
4236 618 604 165 148 30 30 30
30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2842 1186
Size: 1656 4315
BseYI C'CCAG_C
Cuts at: 2226 2462 5174 5670 2226
Size: 236 2712 496 2527
Fragments arranged by size:
2712 2527 496 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2539 2558 3040 1163
Size: 573 803 19 482 4094
Fragments arranged by size:
4094 803 573 482 19
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4182 5210 1540
Size: 275 186 1420 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2977 4032 4117 5188 2977
Size: 1055 85 1071 3760
Fragments arranged by size:
3760 1071 1055 85
BsmI GAATG_Cn'
Cuts at: 2036 3482 3575 2036
Size: 1446 93 4432
Fragments arranged by size:
4432 1446 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2385 2469 2977 3290 4032 4117 5188
Size: 399 595 84 508 313 742 85 1071
Cuts at: 5188 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 595 508 399 313 85
84
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 3036 3078 3860 5590 3036
Size: 42 782 1730 3417
Fragments arranged by size:
3417 1730 782 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2314 2616 2695 2917 3326 844
Size: 1470 302 79 222 409 3489
Fragments arranged by size:
3489 1470 409 302 222 79
BsrBI CCG'CTC
Cuts at: 1394 1823 3858 1394
Size: 429 2035 3507
Fragments arranged by size:
3507 2035 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2193 4466 4640 65
Size: 1183 945 2273 174 1396
Fragments arranged by size:
2273 1396 1183 945 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3227 4615 4802 1700
Size: 110 1417 1388 187 2869
Fragments arranged by size:
2869 1417 1388 187 110
BsrGI T'GTAC_A
Cuts at: 96 3069 96
Size: 2973 2998
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5971
BstBI TT'CG_AA
Cuts at: 4775 4775
Size: 5971
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5971
BstYI r'GATC_y
Cuts at: 1972 2943 3301 3668 4053 4070 5522 5608 5620
Size: 971 358 367 385 17 1452 86 12
Cuts at: 5620 5966 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 971 385 367 358 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2277 2330 2749 513
Size: 552 939 273 53 419 3735
Fragments arranged by size:
3735 939 552 419 273 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5232
Fragments arranged by size:
5232 683 56
BtsI GCAGTG_nn'
Cuts at: 2374 2793 3487 4281 4301 2374
Size: 419 694 794 20 4044
Fragments arranged by size:
4044 794 694 419 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5936
DraI TTT'AAA
Cuts at: 2451 3275 2451
Size: 824 5147
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3225 3418 4307
Size: 54 1425 50 275 18 1395 193 889
Cuts at: 4307 5936 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1395 889 275 193 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3035 3254 3901 1353
Size: 1682 219 647 3423
Fragments arranged by size:
3423 1682 647 219
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4558 4932 5078 387
Size: 1064 138 2969 374 146 1280
Fragments arranged by size:
2969 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2367 2375 3009 4034 4631 5418 5686
Size: 393 735 8 634 1025 597 787 268
Cuts at: 5686 1239
Size: 1524
Fragments arranged by size:
1524 1025 787 735 634 597 393 268
8
EcoO109I rG'GnC_Cy
Cuts at: 2218 2413 2605 2707 2218
Size: 195 192 102 5482
Fragments arranged by size:
5482 195 192 102
EcoRI G'AATT_C
Cuts at: 2723 3280 3658 2723
Size: 557 378 5036
Fragments arranged by size:
5036 557 378
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 2720 3097 5118 1678
Size: 1042 377 2021 2531
Fragments arranged by size:
2531 2021 1042 377
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2832 2864 2913 2918 2945
Size: 32 135 32 1260 32 49 5 27
Cuts at: 2945 2950 2982 3101 3133 4612 4644 4686 4718
Size: 5 32 119 32 1479 32 42 32
Cuts at: 4718 1373
Size: 2626
Fragments arranged by size:
2626 1479 1260 135 119 49 42 32
32 32 32 32 32 32 27 5
5
HincII GTy'rAC
Cuts at: 669 1657 1938 3315 3561 669
Size: 988 281 1377 246 3079
Fragments arranged by size:
3079 1377 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 3206 3296 3206
Size: 90 5881
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2565 5085 5269 1305
Size: 1260 2520 184 2007
Fragments arranged by size:
2520 2007 1260 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4149
Fragments arranged by size:
4149 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2754 3236 3929 4288 4447 518
Size: 648 843 745 482 693 359 159 2042
Fragments arranged by size:
2042 843 745 693 648 482 359 159
MspA1I CmG'CkG
Cuts at: 1606 2332 2462 2542 3221 3387 4064 5212 5457
Size: 726 130 80 679 166 677 1148 245
Cuts at: 5457 1606
Size: 2120
Fragments arranged by size:
2120 1148 726 679 677 245 166 130
80
NaeI GCC'GGC
Cuts at: 3229 3229
Size: 5971
NcoI C'CATG_G
Cuts at: 513 1065 2004 2749 513
Size: 552 939 745 3735
Fragments arranged by size:
3735 939 745 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NgoMIV G'CCGG_C
Cuts at: 3227 3227
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 2912 1052
Size: 1860 4111
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 3268 3329 4874 1132
Size: 97 2039 61 1545 2229
Fragments arranged by size:
2229 2039 1545 97 61
PasI CC'CwG_GG
Cuts at: 1348 2599 1348
Size: 1251 4720
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5971
PfoI T'CCnGG_A
Cuts at: 2025 2061 2025
Size: 36 5935
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2883 2915 4040 4072 5553 5585 2883
Size: 32 1125 32 1481 32 3269
Fragments arranged by size:
3269 1481 1125 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2218 2413 2605 2707 2218
Size: 195 192 102 5482
Fragments arranged by size:
5482 195 192 102
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PspOMI G'GGCC_C
Cuts at: 2381 2381
Size: 5971
PstI C_TGCA'G
Cuts at: 830 2325 2733 3166 3323 830
Size: 1495 408 433 157 3478
Fragments arranged by size:
3478 1495 433 408 157
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 2462 2542 3221 2462
Size: 80 679 5212
Fragments arranged by size:
5212 679 80
SacII CC_GC'GG
Cuts at: 2333 2333
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SanDI GG'GwC_CC
Cuts at: 2413 2413
Size: 5971
SbfI CC_TGCA'GG
Cuts at: 2733 3323 2733
Size: 590 5381
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SexAI A'CCwGG_T
Cuts at: 2532 2532
Size: 5971
SfcI C'TryA_G
Cuts at: 826 1045 2321 2729 3162 3259 3319 4454 5135
Size: 219 1276 408 433 97 60 1135 681
Cuts at: 5135 5326 826
Size: 191 1471
Fragments arranged by size:
1471 1276 1135 681 433 408 219 191
97 60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2187 2264 2441 2873 4075 4976
Size: 197 926 244 77 177 432 1202 901
Cuts at: 4976 5238 820
Size: 262 1553
Fragments arranged by size:
1553 1202 926 901 432 262 244 197
177 77
SnaBI TAC'GTA
Cuts at: 493 3762 493
Size: 3269 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3268 3329 1132
Size: 2136 61 3774
Fragments arranged by size:
3774 2136 61
SspI AAT'ATT
Cuts at: 5 52 2773 3895 5
Size: 47 2721 1122 2081
Fragments arranged by size:
2721 2081 1122 47
StuI AGG'CCT
Cuts at: 1517 2817 1517
Size: 1300 4671
StyI C'CwwG_G
Cuts at: 513 1065 2004 2213 2749 3410 513
Size: 552 939 209 536 661 3074
Fragments arranged by size:
3074 939 661 552 536 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3998 4015 4168 4353 4798 325
Size: 1255 2418 17 153 185 445 1498
Fragments arranged by size:
2418 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3069 4217 5929
Size: 274 80 33 51 1443 1092 1148 1712
Cuts at: 5929 96
Size: 138
Fragments arranged by size:
1712 1443 1148 1092 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2007 2039 2235 2267 5931 5963 2007
Size: 32 196 32 3664 32 2015
Fragments arranged by size:
3664 2015 196 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2308 2308
Size: 5971
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 2286 2895 4100 2286
Size: 609 1205 4157
Fragments arranged by size:
4157 1205 609
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BbeI BbvCI BfrBI BsiWI BsmBI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SapI SfiI SfoI SrfI SwaI