Restriction map for
pFN21AB8675
(Circular) (Six-base) MAPSORT of: pFN21AB8675.seq Check: 4418 from: 1 to: 6571
pFN21AB8675 6571 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2287 2796 2287
Size: 509 6062
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2481 2699 4381 278
Size: 53 83 186 936 945 218 1682 2468
Fragments arranged by size:
2468 1682 945 936 218 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3333 3914 1937
Size: 1396 581 4594
Fragments arranged by size:
4594 1396 581
Acc65I G'GTAC_C
Cuts at: 3892 3892
Size: 6571
AclI AA'CG_TT
Cuts at: 1563 4698 5071 1563
Size: 3135 373 3063
Fragments arranged by size:
3135 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3155 4634 6018 6286 1632
Size: 1523 1479 1384 268 1917
Fragments arranged by size:
1917 1523 1479 1384 268
AfeI AGC'GCT
Cuts at: 2174 2174
Size: 6571
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6374
AflIII A'CryG_T
Cuts at: 1203 5470 6447 1203
Size: 4267 977 1327
Fragments arranged by size:
4267 1327 977
AgeI A'CCGG_T
Cuts at: 5402 5402
Size: 6571
AhdI GACnn_n'nnGTC
Cuts at: 5300 5300
Size: 6571
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6571
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2201 3695 1184
Size: 1017 1494 4060
Fragments arranged by size:
4060 1494 1017
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2489 2521 2489
Size: 32 6539
AlwNI CAG_nnn'CTG
Cuts at: 2086 2287 2329 2362 4314 5886 2086
Size: 201 42 33 1952 1572 2771
Fragments arranged by size:
2771 1952 1572 201 42 33
ApaI G_GGCC'C
Cuts at: 3084 3376 3084
Size: 292 6279
ApaLI G'TGCA_C
Cuts at: 4628 5784 4628
Size: 1156 5415
ApoI r'AATT_y
Cuts at: 1493 3880 4106 4258 6367 1493
Size: 2387 226 152 2109 1697
Fragments arranged by size:
2387 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5125 160
Size: 872 4093 1606
Fragments arranged by size:
4093 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
AvaI C'yCGr_G
Cuts at: 1870 1943 2393 3077 3572 3896 1870
Size: 73 450 684 495 324 4545
Fragments arranged by size:
4545 684 495 450 324 73
BamHI G'GATC_C
Cuts at: 3901 4268 3901
Size: 367 6204
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2063 3384 3545 3704 3892
Size: 325 217 627 276 1321 161 159 188
Cuts at: 3892 5347 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1321 627 325 276 217 188
161 159
BanII G_rGCy'C
Cuts at: 1391 2531 3084 3376 3720 3890 1391
Size: 1140 553 292 344 170 4072
Fragments arranged by size:
4072 1140 553 344 292 170
BbeI G_GCGC'C
Cuts at: 3388 3388
Size: 6571
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2454 2688 2699 928
Size: 971 555 234 11 4800
Fragments arranged by size:
4800 971 555 234 11
BbvCI CC'TCA_GC
Cuts at: 3024 3024
Size: 6571
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4762 4796 1903
Size: 34 2825 34 3678
Fragments arranged by size:
3678 2825 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4463 5673 4463
Size: 1210 5361
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6571
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2120 5182 136
Size: 107 122 71 631 605 448 3062 1525
Fragments arranged by size:
3062 1525 631 605 448 122 107 71
BglII A'GATC_T
Cuts at: 3060 6566 3060
Size: 3506 3065
BlpI GC'TnA_GC
Cuts at: 2039 2907 2984 3988 2039
Size: 868 77 1004 4622
Fragments arranged by size:
4622 1004 868 77
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2116 2929 3055 3084 3376 3707 4632
Size: 276 50 813 126 29 292 331 925
Cuts at: 4632 5788 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 925 813 331 292 276 126
50 29
BmgBI CAC'GTC
Cuts at: 1318 3580 1318
Size: 2262 4309
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2834 4302 5260
Size: 416 53 83 399 24 1417 1468 958
Cuts at: 5260 442
Size: 1753
Fragments arranged by size:
1753 1468 1417 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6571
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2179 3226 2179
Size: 1047 5524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2708 2951 3077 3813 3814 5231 1239
Size: 869 600 243 126 736 1 1417 2579
Fragments arranged by size:
2579 1417 869 736 600 243 126 1
Bpu10I CC'TnA_GC
Cuts at: 3024 3024
Size: 6571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4696 5561 5859 3643
Size: 1053 865 298 4355
Fragments arranged by size:
4355 1053 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3155 3507 3809 5234 882
Size: 617 1656 352 302 1425 2219
Fragments arranged by size:
2219 1656 1425 617 352 302
BsaAI yAC'GTr
Cuts at: 493 1204 4362 493
Size: 711 3158 2702
Fragments arranged by size:
3158 2702 711
BsaBI GATnn'nnATC
Cuts at: 6565 6565
Size: 6571
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2305 2451 2478 2696
Size: 53 83 186 936 772 146 27 218
Cuts at: 2696 3385 4378 4760 5395 275
Size: 689 993 382 635 1451
Fragments arranged by size:
1451 993 936 772 689 635 382 218
186 146 83 53 27
BsaWI w'CCGG_w
Cuts at: 5004 5402 5676 5823 6234 5004
Size: 398 274 147 411 5341
Fragments arranged by size:
5341 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2309 2339 2541 2571 2681
Size: 30 165 30 906 30 202 30 110
Cuts at: 2681 2711 3152 3182 1178
Size: 30 441 30 4567
Fragments arranged by size:
4567 906 441 202 165 110 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2115 2351 2411 2414 2480 2562 3041
Size: 859 70 236 60 3 66 82 479
Cuts at: 3041 3266 1186
Size: 225 4491
Fragments arranged by size:
4491 859 479 236 225 82 70 66
60 3
BseYI C'CCAG_C
Cuts at: 2059 2218 2388 2530 2839 3539 3786 5774 6270
Size: 159 170 142 309 700 247 1988 496
Cuts at: 6270 2059
Size: 2360
Fragments arranged by size:
2360 1988 700 496 309 247 170 159
142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2333 2591 1163
Size: 573 597 258 5143
Fragments arranged by size:
5143 597 573 258
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4021 4782 5810 1540
Size: 275 186 2020 761 1028 2301
Fragments arranged by size:
2301 2020 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4632 4717 5788 4632
Size: 85 1071 5415
Fragments arranged by size:
5415 1071 85
BsmI GAATG_Cn'
Cuts at: 4082 4175 4082
Size: 93 6478
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2116 2531 2929 3055 3084 3376
Size: 399 276 50 415 398 126 29 292
Cuts at: 3376 3707 3720 3890 4632 4717 5788 1391
Size: 331 13 170 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 415 399 398 331 292
276 170 126 85 50 29 13
BspEI T'CCGG_A
Cuts at: 6234 6234
Size: 6571
BspHI T'CATG_A
Cuts at: 2421 3193 4460 6190 2421
Size: 772 1267 1730 2802
Fragments arranged by size:
2802 1730 1267 772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2287 2796 3926 844
Size: 1443 509 1130 3489
Fragments arranged by size:
3489 1443 1130 509
BsrBI CCG'CTC
Cuts at: 1394 1823 3256 4458 1394
Size: 429 1433 1202 3507
Fragments arranged by size:
3507 1433 1202 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5066 5240 65
Size: 1183 3818 174 1396
Fragments arranged by size:
3818 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2144 3381 5215 5402 1700
Size: 110 334 1237 1834 187 2869
Fragments arranged by size:
2869 1834 1237 334 187 110
BsrGI T'GTAC_A
Cuts at: 96 3168 96
Size: 3072 3499
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6571
BssSI C'ACGA_G
Cuts at: 4631 5643 4631
Size: 1012 5559
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2287 2362 2413 794
Size: 1493 75 51 4952
Fragments arranged by size:
4952 1493 75 51
BstBI TT'CG_AA
Cuts at: 5375 5375
Size: 6571
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6571
BstXI CCAn_nnnn'nTGG
Cuts at: 3838 3838
Size: 6571
BstYI r'GATC_y
Cuts at: 1972 2430 2583 2934 3060 3177 3901 4268 4653
Size: 458 153 351 126 117 724 367 385
Cuts at: 4653 4670 6122 6208 6220 6566 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 724 458 385 367 351 346
153 126 117 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2139 3662 2139
Size: 1523 5048
BtgI C'CryG_G
Cuts at: 513 1065 2004 2024 2382 2844 3837 513
Size: 552 939 20 358 462 993 3247
Fragments arranged by size:
3247 993 939 552 462 358 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5832
Fragments arranged by size:
5832 683 56
BtsI GCAGTG_nn'
Cuts at: 2377 3505 3619 3679 4087 4881 4901 2377
Size: 1128 114 60 408 794 20 4047
Fragments arranged by size:
4047 1128 794 408 114 60 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6571
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6536
DraI TTT'AAA
Cuts at: 3875 3875
Size: 6571
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5578 809
Size: 507 4262 1802
Fragments arranged by size:
4262 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2805 2973
Size: 54 1425 50 275 18 177 798 168
Cuts at: 2973 3099 4018 4907 6536 8
Size: 126 919 889 1629 43
Fragments arranged by size:
1629 1425 919 889 798 275 177 168
126 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4018 1537
Size: 275 2206 4090
Fragments arranged by size:
4090 2206 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3771 4501 1353
Size: 2418 730 3423
Fragments arranged by size:
3423 2418 730
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5158 5532 5678 387
Size: 1064 138 3569 374 146 1280
Fragments arranged by size:
3569 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2708 2951 3077 3155 3813 3814
Size: 393 476 600 243 126 78 658 1
Cuts at: 3814 4634 5231 6018 6286 1239
Size: 820 597 787 268 1524
Fragments arranged by size:
1524 820 787 658 600 597 476 393
268 243 126 78 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2541 3137 2541
Size: 596 5975
EcoO109I rG'GnC_Cy
Cuts at: 2947 3372 3373 3584 2947
Size: 425 1 211 5934
Fragments arranged by size:
5934 425 211 1
EcoRI G'AATT_C
Cuts at: 3880 4258 3880
Size: 378 6193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 6571
FspI TGC'GCA
Cuts at: 3362 5077 3362
Size: 1715 4856
HaeII r_GCGC'y
Cuts at: 1678 2176 3280 3291 3388 5718 1678
Size: 498 1104 11 97 2330 2531
Fragments arranged by size:
2531 2330 1104 498 97 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2681 2713 5212 5244 5286
Size: 32 135 32 1109 32 2499 32 42
Cuts at: 5286 5318 1373
Size: 32 2626
Fragments arranged by size:
2626 2499 1109 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3349 3915 4161 669
Size: 988 281 1411 566 246 3079
Fragments arranged by size:
3079 1411 988 566 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6571
HpaI GTT'AAC
Cuts at: 4161 4161
Size: 6571
KasI G'GCGC_C
Cuts at: 3384 3384
Size: 6571
KpnI G_GTAC'C
Cuts at: 3896 3896
Size: 6571
MfeI C'AATT_G
Cuts at: 4170 4170
Size: 6571
MluI A'CGCG_T
Cuts at: 6447 6447
Size: 6571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2421 5685 5869 1305
Size: 1116 3264 184 2007
Fragments arranged by size:
3264 2007 1116 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2807 2975 3101 10
Size: 54 1425 343 177 798 168 126 3480
Fragments arranged by size:
3480 1425 798 343 177 168 126 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2015 3239 4529 4888 5047 518
Size: 648 843 6 1224 1290 359 159 2042
Fragments arranged by size:
2042 1290 1224 843 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2026 3312 3593 3633 3987 4664 5812 6057
Size: 420 1286 281 40 354 677 1148 245
Cuts at: 6057 1606
Size: 2120
Fragments arranged by size:
2120 1286 1148 677 420 354 281 245
40
NaeI GCC'GGC
Cuts at: 2146 2146
Size: 6571
NarI GG'CG_CC
Cuts at: 3385 3385
Size: 6571
NcoI C'CATG_G
Cuts at: 513 1065 2004 3837 513
Size: 552 939 1833 3247
Fragments arranged by size:
3247 1833 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6571
NgoMIV G'CCGG_C
Cuts at: 2144 2144
Size: 6571
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6571
NotI GC'GGCC_GC
Cuts at: 4018 4018
Size: 6571
NspI r_CATG'y
Cuts at: 1132 1229 3929 5474 1132
Size: 97 2700 1545 2229
Fragments arranged by size:
2700 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6571
PciI A'CATG_T
Cuts at: 5470 5470
Size: 6571
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2086 1108
Size: 978 5593
PfoI T'CCnGG_A
Cuts at: 2811 2937 3651 2811
Size: 126 714 5731
Fragments arranged by size:
5731 714 126
PmeI GTTT'AAAC
Cuts at: 3875 3875
Size: 6571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4640 4672 6153 6185 4640
Size: 32 1481 32 5026
Fragments arranged by size:
5026 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2947 3584 2947
Size: 637 5934
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6571
PsiI TTA'TAA
Cuts at: 4141 4141
Size: 6571
PspOMI G'GGCC_C
Cuts at: 3080 3372 3080
Size: 292 6279
PspXI vC'TCGA_Gb
Cuts at: 2393 3572 2393
Size: 1179 5392
PstI C_TGCA'G
Cuts at: 830 2367 3699 3923 830
Size: 1537 1332 224 3478
Fragments arranged by size:
3478 1537 1332 224
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6571
PvuII CAG'CTG
Cuts at: 3312 3593 3312
Size: 281 6290
SacII CC_GC'GG
Cuts at: 2027 2027
Size: 6571
SalI G'TCGA_C
Cuts at: 1936 3913 1936
Size: 1977 4594
SapI GCTCTTCn'nnn_
Cuts at: 3771 3771
Size: 6571
SbfI CC_TGCA'GG
Cuts at: 3923 3923
Size: 6571
ScaI AGT'ACT
Cuts at: 4819 6531 4819
Size: 1712 4859
SexAI A'CCwGG_T
Cuts at: 2613 2613
Size: 6571
SfcI C'TryA_G
Cuts at: 826 1045 2265 2363 3588 3695 3919 5054 5735
Size: 219 1220 98 1225 107 224 1135 681
Cuts at: 5735 5926 826
Size: 191 1471
Fragments arranged by size:
1471 1225 1220 1135 681 224 219 191
107 98
SfoI GGC'GCC
Cuts at: 3386 3386
Size: 6571
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6571
SmaI CCC'GGG
Cuts at: 1872 3898 1872
Size: 2026 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2393 3572 3622 4675 5576 5838
Size: 197 926 450 1179 50 1053 901 262
Cuts at: 5838 820
Size: 1553
Fragments arranged by size:
1553 1179 1053 926 901 450 262 197
50
SnaBI TAC'GTA
Cuts at: 493 4362 493
Size: 3869 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6571
SphI G_CATG'C
Cuts at: 1132 3929 1132
Size: 2797 3774
SspI AAT'ATT
Cuts at: 5 52 3038 4495 5
Size: 47 2986 1457 2081
Fragments arranged by size:
2986 2081 1457 47
StuI AGG'CCT
Cuts at: 1517 3323 1517
Size: 1806 4765
StyI C'CwwG_G
Cuts at: 513 1065 2004 2067 3471 3837 4010 513
Size: 552 939 63 1404 366 173 3074
Fragments arranged by size:
3074 1404 939 552 366 173 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2853 3546 4598 4615 4768 4953 5398
Size: 1255 1273 693 1052 17 153 185 445
Cuts at: 5398 325
Size: 1498
Fragments arranged by size:
1498 1273 1255 1052 693 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2769 2895 3168
Size: 274 80 33 51 1443 792 126 273
Cuts at: 3168 4817 6529 96
Size: 1649 1712 138
Fragments arranged by size:
1712 1649 1443 792 274 273 138 126
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2309 2341 2541 2573 2919 2951 6531 6563 2309
Size: 32 200 32 346 32 3580 32 2317
Fragments arranged by size:
3580 2317 346 200 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6571
XbaI T'CTAG_A
Cuts at: 3907 3907
Size: 6571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2491 3115 2011
Size: 480 624 5467
Fragments arranged by size:
5467 624 480
XhoI C'TCGA_G
Cuts at: 1943 2393 3572 1943
Size: 450 1179 4942
Fragments arranged by size:
4942 1179 450
XmaI C'CCGG_G
Cuts at: 1870 3896 1870
Size: 2026 4545
XmnI GAAnn'nnTTC
Cuts at: 4700 4700
Size: 6571
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2479 2697 4379 276
Size: 53 83 186 936 945 218 1682 2468
Fragments arranged by size:
2468 1682 945 936 218 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI
PsrI RsrII SacI SanDI SfiI SrfI SwaI