Restriction map for
pFN21AB8673
(Circular) (Six-base) MAPSORT of: pFN21AB8673.seq Check: 8329 from: 1 to: 6676
pFN21AB8673 6676 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4486 278
Size: 53 83 186 936 2950 2468
Fragments arranged by size:
2950 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4019 1937
Size: 2082 4594
Acc65I G'GTAC_C
Cuts at: 3997 3997
Size: 6676
AclI AA'CG_TT
Cuts at: 1563 4803 5176 1563
Size: 3240 373 3063
Fragments arranged by size:
3240 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2409 3172 3321 4739 6123 6391 1632
Size: 777 763 149 1418 1384 268 1917
Fragments arranged by size:
1917 1418 1384 777 763 268 149
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6479
AflIII A'CryG_T
Cuts at: 1203 2958 5575 6552 1203
Size: 1755 2617 977 1327
Fragments arranged by size:
2617 1755 1327 977
AgeI A'CCGG_T
Cuts at: 5507 5507
Size: 6676
AhdI GACnn_n'nnGTC
Cuts at: 5405 5405
Size: 6676
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6676
AlwNI CAG_nnn'CTG
Cuts at: 4419 5991 4419
Size: 1572 5104
ApaLI G'TGCA_C
Cuts at: 4733 5889 4733
Size: 1156 5520
ApoI r'AATT_y
Cuts at: 1493 2727 2930 2975 3047 3477 3985 4211 4363
Size: 1234 203 45 72 430 508 226 152
Cuts at: 4363 6472 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1234 508 430 226 203 152
72 45
AseI AT'TA_AT
Cuts at: 160 1032 5230 160
Size: 872 4198 1606
Fragments arranged by size:
4198 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
AvaI C'yCGr_G
Cuts at: 1870 1943 3086 3123 3651 4001 1870
Size: 73 1143 37 528 350 4545
Fragments arranged by size:
4545 1143 528 350 73 37
BamHI G'GATC_C
Cuts at: 4006 4373 4006
Size: 367 6309
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3997 5452 618
Size: 325 217 627 2210 1455 1842
Fragments arranged by size:
2210 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2634 3995 1391
Size: 1243 1361 4072
Fragments arranged by size:
4072 1361 1243
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2120 928
Size: 971 221 5484
Fragments arranged by size:
5484 971 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4867 4901 1903
Size: 34 2930 34 3678
Fragments arranged by size:
3678 2930 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3093 4568 5778 3093
Size: 1475 1210 3991
Fragments arranged by size:
3991 1475 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6676
BfrBI ATG'CAT
Cuts at: 3373 3373
Size: 6676
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5287 136
Size: 107 122 71 631 605 3615 1525
Fragments arranged by size:
3615 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2225 6671 2225
Size: 4446 2230
BlpI GC'TnA_GC
Cuts at: 4093 4093
Size: 6676
Bme1580I G_kGCm'C
Cuts at: 1790 4737 5893 1790
Size: 2947 1156 2573
Fragments arranged by size:
2947 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6676
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2495 4407 5365
Size: 416 53 83 399 24 1078 1912 958
Cuts at: 5365 442
Size: 1753
Fragments arranged by size:
1912 1753 1078 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6676
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2379 5336 1239
Size: 1140 2957 2579
Fragments arranged by size:
2957 2579 1140
Bpu10I CC'TnA_GC
Cuts at: 2028 2028
Size: 6676
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4801 5666 5964 4801
Size: 865 298 5513
Fragments arranged by size:
5513 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3202 5339 882
Size: 617 1703 2137 2219
Fragments arranged by size:
2219 2137 1703 617
BsaAI yAC'GTr
Cuts at: 493 1204 4467 493
Size: 711 3263 2702
Fragments arranged by size:
3263 2702 711
BsaBI GATnn'nnATC
Cuts at: 6670 6670
Size: 6676
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4483 4865 5500 275
Size: 53 83 186 936 2950 382 635 1451
Fragments arranged by size:
2950 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2368 5109 5507 5781 5928 6339 2368
Size: 2741 398 274 147 411 2705
Fragments arranged by size:
2741 2705 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2377 2407 3262 3292 1178
Size: 30 165 30 974 30 855 30 4562
Fragments arranged by size:
4562 974 855 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2049 3270 1186
Size: 863 1221 4592
Fragments arranged by size:
4592 1221 863
BseYI C'CCAG_C
Cuts at: 5879 6375 5879
Size: 496 6180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2066 1163
Size: 573 330 5773
Fragments arranged by size:
5773 573 330
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4126 4887 5915 1540
Size: 275 186 2125 761 1028 2301
Fragments arranged by size:
2301 2125 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2634 2868 4737 4822 5893 2634
Size: 234 1869 85 1071 3417
Fragments arranged by size:
3417 1869 1071 234 85
BsmI GAATG_Cn'
Cuts at: 2953 4187 4280 2953
Size: 1234 93 5349
Fragments arranged by size:
5349 1234 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2634 2868 3995 4737 4822 5893 1391
Size: 399 844 234 1127 742 85 1071 2174
Fragments arranged by size:
2174 1127 1071 844 742 399 234 85
BspEI T'CCGG_A
Cuts at: 6339 6339
Size: 6676
BspHI T'CATG_A
Cuts at: 4565 6295 4565
Size: 1730 4946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4031 844
Size: 3187 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3222 4563 1394
Size: 429 1399 1341 3507
Fragments arranged by size:
3507 1399 1341 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3022 5171 5345 65
Size: 1183 1774 2149 174 1396
Fragments arranged by size:
2149 1774 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5320 5507 1700
Size: 110 3510 187 2869
Fragments arranged by size:
3510 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6676
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6676
BssSI C'ACGA_G
Cuts at: 2669 4736 5748 2669
Size: 2067 1012 3597
Fragments arranged by size:
3597 2067 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6676
BstBI TT'CG_AA
Cuts at: 5480 5480
Size: 6676
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6676
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2805 2011
Size: 794 5882
BstYI r'GATC_y
Cuts at: 1972 2225 4006 4373 4758 4775 6227 6313 6325
Size: 253 1781 367 385 17 1452 86 12
Cuts at: 6325 6671 1972
Size: 346 1977
Fragments arranged by size:
1977 1781 1452 385 367 346 253 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2162 2162
Size: 6676
BtgI C'CryG_G
Cuts at: 513 1065 2004 2283 513
Size: 552 939 279 4906
Fragments arranged by size:
4906 939 552 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5937
Fragments arranged by size:
5937 683 56
BtsI GCAGTG_nn'
Cuts at: 3847 4192 4986 5006 3847
Size: 345 794 20 5517
Fragments arranged by size:
5517 794 345 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6676
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6641
DraI TTT'AAA
Cuts at: 3469 3980 3469
Size: 511 6165
DraIII CAC_nnn'GTG
Cuts at: 3260 3260
Size: 6676
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3653 5683 809
Size: 507 2337 2030 1802
Fragments arranged by size:
2337 2030 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3562 3768 4123
Size: 54 1425 50 275 18 1732 206 355
Cuts at: 4123 5012 6641 8
Size: 889 1629 43
Fragments arranged by size:
1732 1629 1425 889 355 275 206 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4123 1537
Size: 275 2311 4090
Fragments arranged by size:
4090 2311 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2196 2646 3221 4606 1353
Size: 843 450 575 1385 3423
Fragments arranged by size:
3423 1385 843 575 450
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5263 5637 5783 387
Size: 1064 138 3674 374 146 1280
Fragments arranged by size:
3674 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2632 2632
Size: 6676
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2379 2409 3172 3321 4739 5336 6123
Size: 393 747 30 763 149 1418 597 787
Cuts at: 6123 6391 1239
Size: 268 1524
Fragments arranged by size:
1524 1418 787 763 747 597 393 268
149 30
EcoO109I rG'GnC_Cy
Cuts at: 3819 3819
Size: 6676
EcoRI G'AATT_C
Cuts at: 3985 4363 3985
Size: 378 6298
FspI TGC'GCA
Cuts at: 5182 5182
Size: 6676
HaeII r_GCGC'y
Cuts at: 1678 5823 1678
Size: 4145 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2812 2844 3067 3099 5317
Size: 32 135 32 1240 32 223 32 2218
Cuts at: 5317 5349 5391 5423 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2218 1240 223 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3022 4020 4266 669
Size: 988 281 1084 998 246 3079
Fragments arranged by size:
3079 1084 998 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6676
HpaI GTT'AAC
Cuts at: 4266 4266
Size: 6676
KpnI G_GTAC'C
Cuts at: 4001 4001
Size: 6676
MfeI C'AATT_G
Cuts at: 4275 4275
Size: 6676
MluI A'CGCG_T
Cuts at: 6552 6552
Size: 6676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5790 5974 1305
Size: 4485 184 2007
Fragments arranged by size:
4485 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3770 10
Size: 54 1425 343 1938 2916
Fragments arranged by size:
2916 1938 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2957 4634 4993 5152 518
Size: 648 843 948 1677 359 159 2042
Fragments arranged by size:
2042 1677 948 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4092 4769 5917 6162 1606
Size: 2486 677 1148 245 2120
Fragments arranged by size:
2486 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2283 513
Size: 552 939 279 4906
Fragments arranged by size:
4906 939 552 279
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6676
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6676
NotI GC'GGCC_GC
Cuts at: 4123 4123
Size: 6676
NsiI A_TGCA'T
Cuts at: 3375 3375
Size: 6676
NspI r_CATG'y
Cuts at: 1132 1229 3671 4034 5579 1132
Size: 97 2442 363 1545 2229
Fragments arranged by size:
2442 2229 1545 363 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6676
PciI A'CATG_T
Cuts at: 5575 5575
Size: 6676
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3616 1108
Size: 2508 4168
PmeI GTTT'AAAC
Cuts at: 3980 3980
Size: 6676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4745 4777 6258 6290 4745
Size: 32 1481 32 5131
Fragments arranged by size:
5131 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6676
PsiI TTA'TAA
Cuts at: 4246 4246
Size: 6676
PspXI vC'TCGA_Gb
Cuts at: 3651 3651
Size: 6676
PstI C_TGCA'G
Cuts at: 830 3412 4028 830
Size: 2582 616 3478
Fragments arranged by size:
3478 2582 616
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6676
SacI G_AGCT'C
Cuts at: 2634 2634
Size: 6676
SalI G'TCGA_C
Cuts at: 1936 4018 1936
Size: 2082 4594
SbfI CC_TGCA'GG
Cuts at: 4028 4028
Size: 6676
ScaI AGT'ACT
Cuts at: 2626 4924 6636 2626
Size: 2298 1712 2666
Fragments arranged by size:
2666 2298 1712
SfcI C'TryA_G
Cuts at: 826 1045 3055 3408 4024 5159 5840 6031 826
Size: 219 2010 353 616 1135 681 191 1471
Fragments arranged by size:
2010 1471 1135 681 616 353 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6676
SmaI CCC'GGG
Cuts at: 1872 4003 1872
Size: 2131 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3651 4780 5681 5943 820
Size: 197 926 1708 1129 901 262 1553
Fragments arranged by size:
1708 1553 1129 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4467 493
Size: 3974 2702
SpeI A'CTAG_T
Cuts at: 152 2522 152
Size: 2370 4306
SphI G_CATG'C
Cuts at: 1132 3671 4034 1132
Size: 2539 363 3774
Fragments arranged by size:
3774 2539 363
SspI AAT'ATT
Cuts at: 5 52 2181 4600 5
Size: 47 2129 2419 2081
Fragments arranged by size:
2419 2129 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6676
StyI C'CwwG_G
Cuts at: 513 1065 2004 2283 4115 513
Size: 552 939 279 1832 3074
Fragments arranged by size:
3074 1832 939 552 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2514 4703 4720 4873 5058 5503 325
Size: 1255 934 2189 17 153 185 445 1498
Fragments arranged by size:
2189 1498 1255 934 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2240 2624 4922
Size: 274 80 33 51 1443 263 384 2298
Cuts at: 4922 6634 96
Size: 1712 138
Fragments arranged by size:
2298 1712 1443 384 274 263 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2377 2409 3262 3294 6636 6668 2377
Size: 32 853 32 3342 32 2385
Fragments arranged by size:
3342 2385 853 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2543 1316
Size: 1227 5449
XbaI T'CTAG_A
Cuts at: 2194 4012 2194
Size: 1818 4858
XhoI C'TCGA_G
Cuts at: 1943 3651 1943
Size: 1708 4968
XmaI C'CCGG_G
Cuts at: 1870 4001 1870
Size: 2131 4545
XmnI GAAnn'nnTTC
Cuts at: 3050 4805 3050
Size: 1755 4921
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4484 276
Size: 53 83 186 936 2950 2468
Fragments arranged by size:
2950 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI