Restriction map for
pFN21AB8665
(Circular) (Six-base) MAPSORT of: pFN21AB8665.seq Check: 5297 from: 1 to: 6202
pFN21AB8665 6202 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3010 3010
Size: 6202
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4012 278
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2795 3545 1937
Size: 858 750 4594
Fragments arranged by size:
4594 858 750
Acc65I G'GTAC_C
Cuts at: 3078 3523 3078
Size: 445 5757
AclI AA'CG_TT
Cuts at: 1563 4329 4702 1563
Size: 2766 373 3063
Fragments arranged by size:
3063 2766 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2220 2984 4265 5649 5917 1632
Size: 588 764 1281 1384 268 1917
Fragments arranged by size:
1917 1384 1281 764 588 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6005
AflIII A'CryG_T
Cuts at: 1203 5101 6078 1203
Size: 3898 977 1327
Fragments arranged by size:
3898 1327 977
AgeI A'CCGG_T
Cuts at: 5033 5033
Size: 6202
AhdI GACnn_n'nnGTC
Cuts at: 4931 4931
Size: 6202
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2768 1184
Size: 1584 4618
AlwNI CAG_nnn'CTG
Cuts at: 2539 2851 3382 3945 5517 2539
Size: 312 531 563 1572 3224
Fragments arranged by size:
3224 1572 563 531 312
ApaI G_GGCC'C
Cuts at: 2767 2767
Size: 6202
ApaLI G'TGCA_C
Cuts at: 2899 4259 5415 2899
Size: 1360 1156 3686
Fragments arranged by size:
3686 1360 1156
ApoI r'AATT_y
Cuts at: 1493 2984 3511 3737 3889 5998 1493
Size: 1491 527 226 152 2109 1697
Fragments arranged by size:
2109 1697 1491 527 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4756 160
Size: 872 3724 1606
Fragments arranged by size:
3724 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
AvaI C'yCGr_G
Cuts at: 1870 1943 2759 3340 3527 1870
Size: 73 816 581 187 4545
Fragments arranged by size:
4545 816 581 187 73
BamHI G'GATC_C
Cuts at: 3532 3899 3532
Size: 367 5835
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2734 3078 3523 4978 618
Size: 325 217 627 947 344 445 1455 1842
Fragments arranged by size:
1842 1455 947 627 445 344 325 217
BanII G_rGCy'C
Cuts at: 1391 2025 2767 3521 1391
Size: 634 742 754 4072
Fragments arranged by size:
4072 754 742 634
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5231
BbvCI CC'TCA_GC
Cuts at: 3423 3423
Size: 6202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4393 4427 1903
Size: 34 2456 34 3678
Fragments arranged by size:
3678 2456 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4094 5304 4094
Size: 1210 4992
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6202
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3201 3254 4813
Size: 107 122 71 631 605 1529 53 1559
Cuts at: 4813 136
Size: 1525
Fragments arranged by size:
1559 1529 1525 631 605 122 107 71
53
BglII A'GATC_T
Cuts at: 6197 6197
Size: 6202
BlpI GC'TnA_GC
Cuts at: 3619 3619
Size: 6202
Bme1580I G_kGCm'C
Cuts at: 1790 2739 2767 2903 3347 4263 5419 1790
Size: 949 28 136 444 916 1156 2573
Fragments arranged by size:
2573 1156 949 916 444 136 28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6202
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3933 4891 442
Size: 416 53 83 399 24 2516 958 1753
Fragments arranged by size:
2516 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2399 2576 2585 2609 3337 3359 3404 3430
Size: 1160 177 9 24 728 22 45 26
Cuts at: 3430 4862 1239
Size: 1432 2579
Fragments arranged by size:
2579 1432 1160 728 177 45 26 24
22 9
Bpu10I CC'TnA_GC
Cuts at: 3423 3423
Size: 6202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3449 4327 5192 5490 3449
Size: 878 865 298 4161
Fragments arranged by size:
4161 878 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4865 882
Size: 617 3366 2219
Fragments arranged by size:
3366 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3993 493
Size: 711 2789 2702
Fragments arranged by size:
2789 2702 711
BsaBI GATnn'nnATC
Cuts at: 6196 6196
Size: 6202
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3162 4009 4391 5026
Size: 53 83 186 936 1629 847 382 635
Cuts at: 5026 275
Size: 1451
Fragments arranged by size:
1629 1451 936 847 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2857 3117 4635 5033 5307 5454 5865 2857
Size: 260 1518 398 274 147 411 3194
Fragments arranged by size:
3194 1518 411 398 274 260 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5977
Fragments arranged by size:
5977 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2102 2129 2222 2561 2564 2582 3170
Size: 883 33 27 93 339 3 18 588
Cuts at: 3170 3328 1186
Size: 158 4060
Fragments arranged by size:
4060 883 588 339 158 93 33 27
18 3
BseYI C'CCAG_C
Cuts at: 2428 2766 3100 5405 5901 2428
Size: 338 334 2305 496 2729
Fragments arranged by size:
2729 2305 496 338 334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2129 2864 1163
Size: 573 393 735 4501
Fragments arranged by size:
4501 735 573 393
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2100 2688 3073 3334 3652 4413
Size: 275 186 99 588 385 261 318 761
Cuts at: 4413 5441 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 588 385 318 275 261
186 99
BsiHKAI G_wGCw'C
Cuts at: 2025 2903 2939 4263 4348 5419 2025
Size: 878 36 1324 85 1071 2808
Fragments arranged by size:
2808 1324 1071 878 85 36
BsmI GAATG_Cn'
Cuts at: 3713 3806 3713
Size: 93 6109
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2739 2767 2903 2939 3347 3521
Size: 399 235 714 28 136 36 408 174
Cuts at: 3521 4263 4348 5419 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 714 408 399 235 174
136 85 36 28
BspEI T'CCGG_A
Cuts at: 3117 5865 3117
Size: 2748 3454
BspHI T'CATG_A
Cuts at: 4091 5821 4091
Size: 1730 4472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2475 3010 3066 3557 844
Size: 1631 535 56 491 3489
Fragments arranged by size:
3489 1631 535 491 56
BsrBI CCG'CTC
Cuts at: 1394 1823 2560 3097 3336 4089 1394
Size: 429 737 537 239 753 3507
Fragments arranged by size:
3507 753 737 537 429 239
BsrDI GCAATG_nn'
Cuts at: 65 1248 4697 4871 65
Size: 1183 3449 174 1396
Fragments arranged by size:
3449 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2708 2816 4846 5033 1700
Size: 110 898 108 2030 187 2869
Fragments arranged by size:
2869 2030 898 187 110 108
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6202
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6202
BssSI C'ACGA_G
Cuts at: 2930 4262 5274 2930
Size: 1332 1012 3858
Fragments arranged by size:
3858 1332 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2851 794
Size: 2057 4145
BstBI TT'CG_AA
Cuts at: 5006 5006
Size: 6202
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6202
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2401 2497 2698 2011
Size: 390 96 201 5515
Fragments arranged by size:
5515 390 201 96
BstYI r'GATC_y
Cuts at: 1972 2128 2475 2860 3114 3261 3532 3899 4284
Size: 156 347 385 254 147 271 367 385
Cuts at: 4284 4301 5753 5839 5851 6197 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 385 367 347 346 271
254 156 147 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2304 2682 513
Size: 552 1239 378 4033
Fragments arranged by size:
4033 1239 552 378
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2140 2632 487
Size: 683 56 914 492 4057
Fragments arranged by size:
4057 914 683 492 56
BtsI GCAGTG_nn'
Cuts at: 3220 3718 4512 4532 3220
Size: 498 794 20 4890
Fragments arranged by size:
4890 794 498 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6202
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6167
DraI TTT'AAA
Cuts at: 3506 3506
Size: 6202
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5209 809
Size: 507 3893 1802
Fragments arranged by size:
3893 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2685 2706 2889
Size: 54 1425 50 275 18 855 21 183
Cuts at: 2889 3070 3649 4538 6167 8
Size: 181 579 889 1629 43
Fragments arranged by size:
1629 1425 889 855 579 275 183 181
54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2685 3070 3649 1537
Size: 275 873 385 579 4090
Fragments arranged by size:
4090 873 579 385 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2034 2046 2079 3476 4132 1353
Size: 681 12 33 1397 656 3423
Fragments arranged by size:
3423 1397 681 656 33 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2884 4789 5163 5309 387
Size: 1064 138 1295 1905 374 146 1280
Fragments arranged by size:
1905 1295 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2023 2023
Size: 6202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2220 2399 2576 2585 2609 2984 3337
Size: 393 588 179 177 9 24 375 353
Cuts at: 3337 3359 3404 3430 4265 4862 5649 5917 1239
Size: 22 45 26 835 597 787 268 1524
Fragments arranged by size:
1524 835 787 597 588 393 375 353
268 179 177 45 26 24 22 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2729 2729
Size: 6202
EcoO109I rG'GnC_Cy
Cuts at: 2763 3175 3379 2763
Size: 412 204 5586
Fragments arranged by size:
5586 412 204
EcoRI G'AATT_C
Cuts at: 3511 3889 3511
Size: 378 5824
FspI TGC'GCA
Cuts at: 4708 4708
Size: 6202
HaeII r_GCGC'y
Cuts at: 1678 2588 5349 1678
Size: 910 2761 2531
Fragments arranged by size:
2761 2531 910
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2342 2374 3323 3355 4843
Size: 32 135 32 770 32 949 32 1488
Cuts at: 4843 4875 4917 4949 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1488 949 770 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3546 3792 669
Size: 988 281 1608 246 3079
Fragments arranged by size:
3079 1608 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2232 748
Size: 1484 4718
HpaI GTT'AAC
Cuts at: 3792 3792
Size: 6202
KpnI G_GTAC'C
Cuts at: 3082 3527 3082
Size: 445 5757
MfeI C'AATT_G
Cuts at: 3801 3801
Size: 6202
MluI A'CGCG_T
Cuts at: 6078 6078
Size: 6202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2523 5316 5500 1305
Size: 1218 2793 184 2007
Fragments arranged by size:
2793 2007 1218 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4380
Fragments arranged by size:
4380 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 2702 4160 4519 4678 518
Size: 648 1329 207 1458 359 159 2042
Fragments arranged by size:
2042 1458 1329 648 359 207 159
MspA1I CmG'CkG
Cuts at: 1606 2125 2539 3031 3055 3280 3618 4295 5443
Size: 519 414 492 24 225 338 677 1148
Cuts at: 5443 5688 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 519 492 414 338 245
225 24
NaeI GCC'GGC
Cuts at: 2710 2710
Size: 6202
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5650
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6202
NgoMIV G'CCGG_C
Cuts at: 2708 2708
Size: 6202
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6202
NotI GC'GGCC_GC
Cuts at: 3070 3649 3070
Size: 579 5623
NspI r_CATG'y
Cuts at: 1132 1229 3560 5105 1132
Size: 97 2331 1545 2229
Fragments arranged by size:
2331 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2527 1348
Size: 1179 5023
PciI A'CATG_T
Cuts at: 5101 5101
Size: 6202
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2166 3382 1108
Size: 1058 1216 3928
Fragments arranged by size:
3928 1216 1058
PfoI T'CCnGG_A
Cuts at: 2346 2633 2346
Size: 287 5915
PmeI GTTT'AAAC
Cuts at: 3506 3506
Size: 6202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4271 4303 5784 5816 4271
Size: 32 1481 32 4657
Fragments arranged by size:
4657 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3175 3175
Size: 6202
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6202
PsiI TTA'TAA
Cuts at: 3772 3772
Size: 6202
PspOMI G'GGCC_C
Cuts at: 2763 2763
Size: 6202
PstI C_TGCA'G
Cuts at: 830 2472 3279 3291 3554 830
Size: 1642 807 12 263 3478
Fragments arranged by size:
3478 1642 807 263 12
PvuI CG_AT'CG
Cuts at: 2001 2100 2001
Size: 99 6103
PvuII CAG'CTG
Cuts at: 2125 2539 2125
Size: 414 5788
SacI G_AGCT'C
Cuts at: 2025 2025
Size: 6202
SalI G'TCGA_C
Cuts at: 1936 3544 1936
Size: 1608 4594
SbfI CC_TGCA'GG
Cuts at: 3554 3554
Size: 6202
ScaI AGT'ACT
Cuts at: 2357 4450 6162 2357
Size: 2093 1712 2397
Fragments arranged by size:
2397 2093 1712
SfcI C'TryA_G
Cuts at: 826 1045 2468 3275 3287 3550 4685 5366 5557
Size: 219 1423 807 12 263 1135 681 191
Cuts at: 5557 826
Size: 1471
Fragments arranged by size:
1471 1423 1135 807 681 263 219 191
12
SfiI GGCCn_nnn'nGGCC
Cuts at: 3254 3254
Size: 6202
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6202
SmaI CCC'GGG
Cuts at: 1872 3529 1872
Size: 1657 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3464 4306 5207 5469 820
Size: 197 926 1521 842 901 262 1553
Fragments arranged by size:
1553 1521 926 901 842 262 197
SnaBI TAC'GTA
Cuts at: 493 3993 493
Size: 3500 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6202
SphI G_CATG'C
Cuts at: 1132 3560 1132
Size: 2428 3774
SspI AAT'ATT
Cuts at: 5 52 4126 5
Size: 47 4074 2081
Fragments arranged by size:
4074 2081 47
StuI AGG'CCT
Cuts at: 1517 2573 1517
Size: 1056 5146
StyI C'CwwG_G
Cuts at: 513 1065 2385 2730 3252 3641 513
Size: 552 1320 345 522 389 3074
Fragments arranged by size:
3074 1320 552 522 389 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2540 2668 2690 4229 4246 4399 4584
Size: 1255 960 128 22 1539 17 153 185
Cuts at: 4584 5029 325
Size: 445 1498
Fragments arranged by size:
1539 1498 1255 960 445 185 153 128
22 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2355 4448 6160
Size: 274 80 33 51 1443 378 2093 1712
Cuts at: 6160 96
Size: 138
Fragments arranged by size:
2093 1712 1443 378 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6162 6194 6162
Size: 32 6170
Tth111I GACn'n_nGTC
Cuts at: 1316 2779 1316
Size: 1463 4739
XbaI T'CTAG_A
Cuts at: 3538 3538
Size: 6202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2902 2902
Size: 6202
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6202
XmaI C'CCGG_G
Cuts at: 1870 3527 1870
Size: 1657 4545
XmnI GAAnn'nnTTC
Cuts at: 3440 4331 3440
Size: 891 5311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4010 276
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI
SwaI