Restriction map for
pFN21AB8663
(Circular) (Six-base) MAPSORT of: pFN21AB8663.seq Check: 4719 from: 1 to: 6379
pFN21AB8663 6379 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4189 278
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3722 1937
Size: 1785 4594
Acc65I G'GTAC_C
Cuts at: 3700 3700
Size: 6379
AclI AA'CG_TT
Cuts at: 1563 4506 4879 1563
Size: 2943 373 3063
Fragments arranged by size:
3063 2943 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 2493 3327 4442 5826 6094 1632
Size: 491 370 834 1115 1384 268 1917
Fragments arranged by size:
1917 1384 1115 834 491 370 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6182
AflIII A'CryG_T
Cuts at: 1203 5278 6255 1203
Size: 4075 977 1327
Fragments arranged by size:
4075 1327 977
AgeI A'CCGG_T
Cuts at: 5210 5210
Size: 6379
AhdI GACnn_n'nnGTC
Cuts at: 5108 5108
Size: 6379
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6379
AlwNI CAG_nnn'CTG
Cuts at: 4122 5694 4122
Size: 1572 4807
ApaLI G'TGCA_C
Cuts at: 4436 5592 4436
Size: 1156 5223
ApoI r'AATT_y
Cuts at: 1493 3379 3398 3688 3914 4066 6175 1493
Size: 1886 19 290 226 152 2109 1697
Fragments arranged by size:
2109 1886 1697 290 226 152 19
AseI AT'TA_AT
Cuts at: 160 1032 4933 160
Size: 872 3901 1606
Fragments arranged by size:
3901 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
AvaI C'yCGr_G
Cuts at: 1870 1943 3704 1870
Size: 73 1761 4545
Fragments arranged by size:
4545 1761 73
BamHI G'GATC_C
Cuts at: 3709 4076 3709
Size: 367 6012
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2559 2907 3700 5155 618
Size: 325 217 627 772 348 793 1455 1842
Fragments arranged by size:
1842 1455 793 772 627 348 325 217
BanII G_rGCy'C
Cuts at: 1391 3698 1391
Size: 2307 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3333 928
Size: 971 1434 3974
Fragments arranged by size:
3974 1434 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4570 4604 1903
Size: 34 2633 34 3678
Fragments arranged by size:
3678 2633 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4271 5481 4271
Size: 1210 5169
BclI T'GATC_A
Cuts at: 1548 3202 1548
Size: 1654 4725
BfrBI ATG'CAT
Cuts at: 2700 2700
Size: 6379
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4990 136
Size: 107 122 71 631 605 3318 1525
Fragments arranged by size:
3318 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2539 2621 6374 2539
Size: 82 3753 2544
Fragments arranged by size:
3753 2544 82
BlpI GC'TnA_GC
Cuts at: 3796 3796
Size: 6379
Bme1580I G_kGCm'C
Cuts at: 1790 2898 4440 5596 1790
Size: 1108 1542 1156 2573
Fragments arranged by size:
2573 1542 1156 1108
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3210 3500 4110
Size: 416 53 83 399 24 1793 290 610
Cuts at: 4110 5068 442
Size: 958 1753
Fragments arranged by size:
1793 1753 958 610 416 399 290 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6379
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3487 3487
Size: 6379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5039 1239
Size: 3800 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4504 5369 5667 4504
Size: 865 298 5216
Fragments arranged by size:
5216 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2111 2819 2976 3541 5042 882
Size: 617 612 708 157 565 1501 2219
Fragments arranged by size:
2219 1501 708 617 612 565 157
BsaAI yAC'GTr
Cuts at: 493 1204 4170 493
Size: 711 2966 2702
Fragments arranged by size:
2966 2702 711
BsaBI GATnn'nnATC
Cuts at: 6373 6373
Size: 6379
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4186 4568 5203 275
Size: 53 83 186 936 2653 382 635 1451
Fragments arranged by size:
2653 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3015 4812 5210 5484 5631 6042 3015
Size: 1797 398 274 147 411 3352
Fragments arranged by size:
3352 1797 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6154
Fragments arranged by size:
6154 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6379
BseYI C'CCAG_C
Cuts at: 5582 6078 5582
Size: 496 5883
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2487 1163
Size: 573 751 5055
Fragments arranged by size:
5055 751 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3829 4590 5618 1540
Size: 275 186 1828 761 1028 2301
Fragments arranged by size:
2301 1828 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4440 4525 5596 4440
Size: 85 1071 5223
Fragments arranged by size:
5223 1071 85
BsmI GAATG_Cn'
Cuts at: 2700 3890 3983 2700
Size: 1190 93 5096
Fragments arranged by size:
5096 1190 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2898 3698 4440 4525 5596 1391
Size: 399 1108 800 742 85 1071 2174
Fragments arranged by size:
2174 1108 1071 800 742 399 85
BspEI T'CCGG_A
Cuts at: 6042 6042
Size: 6379
BspHI T'CATG_A
Cuts at: 4268 5998 4268
Size: 1730 4649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3734 844
Size: 2890 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4266 1394
Size: 429 2443 3507
Fragments arranged by size:
3507 2443 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4874 5048 65
Size: 1183 3626 174 1396
Fragments arranged by size:
3626 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5023 5210 1700
Size: 110 3213 187 2869
Fragments arranged by size:
3213 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6379
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6379
BssSI C'ACGA_G
Cuts at: 4439 5451 4439
Size: 1012 5367
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2210 794
Size: 1416 4963
BstBI TT'CG_AA
Cuts at: 5183 5183
Size: 6379
BstEII G'GTnAC_C
Cuts at: 1184 2874 1184
Size: 1690 4689
BstXI CCAn_nnnn'nTGG
Cuts at: 3634 3634
Size: 6379
BstYI r'GATC_y
Cuts at: 1972 2539 2621 3416 3709 4076 4461 4478 5930
Size: 567 82 795 293 367 385 17 1452
Cuts at: 5930 6016 6028 6374 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 795 567 385 367 346 293
86 82 17 12
Bsu36I CC'TnA_GG
Cuts at: 3555 3555
Size: 6379
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4888
Fragments arranged by size:
4888 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5640
Fragments arranged by size:
5640 683 56
BtsI GCAGTG_nn'
Cuts at: 2773 3567 3895 4689 4709 2773
Size: 794 328 794 20 4443
Fragments arranged by size:
4443 794 794 328 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6344
DraI TTT'AAA
Cuts at: 3683 3683
Size: 6379
DraIII CAC_nnn'GTG
Cuts at: 2882 2882
Size: 6379
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5386 809
Size: 507 4070 1802
Fragments arranged by size:
4070 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3624 3826 4715
Size: 54 1425 50 275 18 1794 202 889
Cuts at: 4715 6344 8
Size: 1629 43
Fragments arranged by size:
1794 1629 1425 889 275 202 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3826 1537
Size: 275 2014 4090
Fragments arranged by size:
4090 2014 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2580 3068 3231 3252 4309 1353
Size: 1227 488 163 21 1057 3423
Fragments arranged by size:
3423 1227 1057 488 163 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4966 5340 5486 387
Size: 1064 138 3377 374 146 1280
Fragments arranged by size:
3377 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2493 3327 4442 5039 5826 6094
Size: 393 491 370 834 1115 597 787 268
Cuts at: 6094 1239
Size: 1524
Fragments arranged by size:
1524 1115 834 787 597 491 393 370
268
EcoO109I rG'GnC_Cy
Cuts at: 2197 2197
Size: 6379
EcoRI G'AATT_C
Cuts at: 3688 4066 3688
Size: 378 6001
FspI TGC'GCA
Cuts at: 4885 4885
Size: 6379
HaeII r_GCGC'y
Cuts at: 1678 5526 1678
Size: 3848 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2105 2137 5020 5052 5094
Size: 32 135 32 533 32 2883 32 42
Cuts at: 5094 5126 1373
Size: 32 2626
Fragments arranged by size:
2883 2626 533 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3723 3969 669
Size: 988 281 1785 246 3079
Fragments arranged by size:
3079 1785 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6379
HpaI GTT'AAC
Cuts at: 3969 3969
Size: 6379
KpnI G_GTAC'C
Cuts at: 3704 3704
Size: 6379
MfeI C'AATT_G
Cuts at: 3978 3978
Size: 6379
MluI A'CGCG_T
Cuts at: 6255 6255
Size: 6379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3444 5493 5677 1305
Size: 2139 2049 184 2007
Fragments arranged by size:
2139 2049 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3626 10
Size: 54 1425 343 1794 2763
Fragments arranged by size:
2763 1794 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4337 4696 4855 518
Size: 648 3171 359 159 2042
Fragments arranged by size:
3171 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3795 4472 5620 5865 1606
Size: 2189 677 1148 245 2120
Fragments arranged by size:
2189 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4888
Fragments arranged by size:
4888 939 552
NdeI CA'TA_TG
Cuts at: 387 3264 387
Size: 2877 3502
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6379
NotI GC'GGCC_GC
Cuts at: 3826 3826
Size: 6379
NsiI A_TGCA'T
Cuts at: 2702 2702
Size: 6379
NspI r_CATG'y
Cuts at: 1132 1229 2517 3737 5282 1132
Size: 97 1288 1220 1545 2229
Fragments arranged by size:
2229 1545 1288 1220 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6379
PciI A'CATG_T
Cuts at: 5278 5278
Size: 6379
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6379
PmeI GTTT'AAAC
Cuts at: 3683 3683
Size: 6379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4448 4480 5961 5993 4448
Size: 32 1481 32 4834
Fragments arranged by size:
4834 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6379
PsiI TTA'TAA
Cuts at: 3949 3949
Size: 6379
PstI C_TGCA'G
Cuts at: 830 2807 3731 830
Size: 1977 924 3478
Fragments arranged by size:
3478 1977 924
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6379
SalI G'TCGA_C
Cuts at: 1936 3721 1936
Size: 1785 4594
SapI GCTCTTCn'nnn_
Cuts at: 3252 3252
Size: 6379
SbfI CC_TGCA'GG
Cuts at: 3731 3731
Size: 6379
ScaI AGT'ACT
Cuts at: 4627 6339 4627
Size: 1712 4667
SfcI C'TryA_G
Cuts at: 826 1045 2803 3727 4862 5543 5734 826
Size: 219 1758 924 1135 681 191 1471
Fragments arranged by size:
1758 1471 1135 924 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6379
SmaI CCC'GGG
Cuts at: 1872 3706 1872
Size: 1834 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4483 5384 5646 820
Size: 197 926 2540 901 262 1553
Fragments arranged by size:
2540 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4170 493
Size: 3677 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6379
SphI G_CATG'C
Cuts at: 1132 3737 1132
Size: 2605 3774
SspI AAT'ATT
Cuts at: 5 52 3164 4303 5
Size: 47 3112 1139 2081
Fragments arranged by size:
3112 2081 1139 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6379
StyI C'CwwG_G
Cuts at: 513 1065 2004 3098 3818 513
Size: 552 939 1094 720 3074
Fragments arranged by size:
3074 1094 939 720 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2862 4406 4423 4576 4761 5206 325
Size: 1255 1282 1544 17 153 185 445 1498
Fragments arranged by size:
1544 1498 1282 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4625 6337 96
Size: 274 80 33 51 1443 2648 1712 138
Fragments arranged by size:
2648 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6339 6371 6339
Size: 32 6347
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6379
XbaI T'CTAG_A
Cuts at: 3715 3715
Size: 6379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2284 2284
Size: 6379
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6379
XmaI C'CCGG_G
Cuts at: 1870 3704 1870
Size: 1834 4545
XmnI GAAnn'nnTTC
Cuts at: 2136 2840 4508 2136
Size: 704 1668 4007
Fragments arranged by size:
4007 1668 704
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4187 276
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI