Restriction map for
pFN21AB8661
(Circular) (Six-base) MAPSORT of: pFN21AB8661.seq Check: 9938 from: 1 to: 5965
pFN21AB8661 5965 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3775 278
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3308 1937
Size: 1371 4594
Acc65I G'GTAC_C
Cuts at: 3286 3286
Size: 5965
AclI AA'CG_TT
Cuts at: 1563 4092 4465 1563
Size: 2529 373 3063
Fragments arranged by size:
3063 2529 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2229 4028 5412 5680 1632
Size: 597 1799 1384 268 1917
Fragments arranged by size:
1917 1799 1384 597 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5768
AflIII A'CryG_T
Cuts at: 1203 2081 3150 4864 5841 1203
Size: 878 1069 1714 977 1327
Fragments arranged by size:
1714 1327 1069 977 878
AgeI A'CCGG_T
Cuts at: 4796 4796
Size: 5965
AhdI GACnn_n'nnGTC
Cuts at: 4694 4694
Size: 5965
AleI CACnn'nnGTG
Cuts at: 1166 2792 1166
Size: 1626 4339
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5965
AlwNI CAG_nnn'CTG
Cuts at: 2966 3708 5280 2966
Size: 742 1572 3651
Fragments arranged by size:
3651 1572 742
ApaLI G'TGCA_C
Cuts at: 4022 5178 4022
Size: 1156 4809
ApoI r'AATT_y
Cuts at: 1493 3274 3500 3652 5761 1493
Size: 1781 226 152 2109 1697
Fragments arranged by size:
2109 1781 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4519 160
Size: 872 3487 1606
Fragments arranged by size:
3487 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
AvaI C'yCGr_G
Cuts at: 1870 1943 3290 1870
Size: 73 1347 4545
Fragments arranged by size:
4545 1347 73
BamHI G'GATC_C
Cuts at: 3295 3662 3295
Size: 367 5598
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2175 3286 4741 618
Size: 325 217 627 388 1111 1455 1842
Fragments arranged by size:
1842 1455 1111 627 388 325 217
BanII G_rGCy'C
Cuts at: 1391 3007 3193 3241 3284 1391
Size: 1616 186 48 43 4072
Fragments arranged by size:
4072 1616 186 48 43
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2210 928
Size: 971 311 4683
Fragments arranged by size:
4683 971 311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4156 4190 1903
Size: 34 2219 34 3678
Fragments arranged by size:
3678 2219 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3857 5067 3857
Size: 1210 4755
BclI T'GATC_A
Cuts at: 1548 2587 1548
Size: 1039 4926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4576 136
Size: 107 122 71 631 605 2904 1525
Fragments arranged by size:
2904 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5960 5960
Size: 5965
BlpI GC'TnA_GC
Cuts at: 3382 3382
Size: 5965
Bme1580I G_kGCm'C
Cuts at: 1790 2180 4026 5182 1790
Size: 390 1846 1156 2573
Fragments arranged by size:
2573 1846 1156 390
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5965
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2056 3696 4654
Size: 416 53 83 399 24 639 1640 958
Cuts at: 4654 442
Size: 1753
Fragments arranged by size:
1753 1640 958 639 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2202 3060 3177 4625 1239
Size: 963 858 117 1448 2579
Fragments arranged by size:
2579 1448 963 858 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4090 4955 5253 4090
Size: 865 298 4802
Fragments arranged by size:
4802 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4628 882
Size: 617 3129 2219
Fragments arranged by size:
3129 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3756 493
Size: 711 2552 2702
Fragments arranged by size:
2702 2552 711
BsaBI GATnn'nnATC
Cuts at: 5959 5959
Size: 5965
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3772 4154 4789 275
Size: 53 83 186 936 2239 382 635 1451
Fragments arranged by size:
2239 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2778 4398 4796 5070 5217 5628 2778
Size: 1620 398 274 147 411 3115
Fragments arranged by size:
3115 1620 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2483 2513 1178
Size: 30 165 30 1080 30 4630
Fragments arranged by size:
4630 1080 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2544 3003 3006 1186
Size: 1358 459 3 4145
Fragments arranged by size:
4145 1358 459 3
BseYI C'CCAG_C
Cuts at: 2413 5168 5664 2413
Size: 2755 496 2714
Fragments arranged by size:
2755 2714 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2816 1163
Size: 573 1080 4312
Fragments arranged by size:
4312 1080 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3415 4176 5204 1540
Size: 275 186 1414 761 1028 2301
Fragments arranged by size:
2301 1414 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3007 3241 4026 4111 5182 3007
Size: 234 785 85 1071 3790
Fragments arranged by size:
3790 1071 785 234 85
BsmI GAATG_Cn'
Cuts at: 2141 3476 3569 2141
Size: 1335 93 4537
Fragments arranged by size:
4537 1335 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2180 3007 3193 3241 3284 4026 4111
Size: 399 390 827 186 48 43 742 85
Cuts at: 4111 5182 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 827 742 399 390 186 85
48 43
BspEI T'CCGG_A
Cuts at: 2778 5628 2778
Size: 2850 3115
BspHI T'CATG_A
Cuts at: 3854 5584 3854
Size: 1730 4235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2231 2261 3320 844
Size: 1387 30 1059 3489
Fragments arranged by size:
3489 1387 1059 30
BsrBI CCG'CTC
Cuts at: 1394 1823 3852 1394
Size: 429 2029 3507
Fragments arranged by size:
3507 2029 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3090 3114 4460 4634 65
Size: 1183 1842 24 1346 174 1396
Fragments arranged by size:
1842 1396 1346 1183 174 24
BsrFI r'CCGG_y
Cuts at: 1700 1810 4609 4796 1700
Size: 110 2799 187 2869
Fragments arranged by size:
2869 2799 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5965
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5965
BssSI C'ACGA_G
Cuts at: 4025 5037 4025
Size: 1012 4953
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5965
BstBI TT'CG_AA
Cuts at: 4769 4769
Size: 5965
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5965
BstYI r'GATC_y
Cuts at: 1972 2566 2775 2915 3295 3662 4047 4064 5516
Size: 594 209 140 380 367 385 17 1452
Cuts at: 5516 5602 5614 5960 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 594 385 380 367 346 209
140 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4474
Fragments arranged by size:
4474 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5226
Fragments arranged by size:
5226 683 56
BtsI GCAGTG_nn'
Cuts at: 2813 3481 4275 4295 2813
Size: 668 794 20 4483
Fragments arranged by size:
4483 794 668 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5930
DraI TTT'AAA
Cuts at: 2314 3269 2314
Size: 955 5010
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4972 809
Size: 507 3656 1802
Fragments arranged by size:
3656 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2399 2887 3412
Size: 54 1425 50 275 18 569 488 525
Cuts at: 3412 4301 5930 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 569 525 488 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3412 1537
Size: 275 1600 4090
Fragments arranged by size:
4090 1600 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3012 3895 1353
Size: 1659 883 3423
Fragments arranged by size:
3423 1659 883
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4552 4926 5072 387
Size: 1064 138 2963 374 146 1280
Fragments arranged by size:
2963 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3005 3239 3005
Size: 234 5731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2202 2229 3060 3177 4028 4625 5412
Size: 393 570 27 831 117 851 597 787
Cuts at: 5412 5680 1239
Size: 268 1524
Fragments arranged by size:
1524 851 831 787 597 570 393 268
117 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2035 2988 2035
Size: 953 5012
EcoO109I rG'GnC_Cy
Cuts at: 3220 3220
Size: 5965
EcoRI G'AATT_C
Cuts at: 3274 3652 3274
Size: 378 5587
FspI TGC'GCA
Cuts at: 4471 4471
Size: 5965
HaeII r_GCGC'y
Cuts at: 1678 5112 1678
Size: 3434 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3223 3255 4606 4638 4680
Size: 32 135 32 1651 32 1351 32 42
Cuts at: 4680 4712 1373
Size: 32 2626
Fragments arranged by size:
2626 1651 1351 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2341 3309 3555 669
Size: 988 281 403 968 246 3079
Fragments arranged by size:
3079 988 968 403 281 246
HindIII A'AGCT_T
Cuts at: 748 2639 748
Size: 1891 4074
HpaI GTT'AAC
Cuts at: 3555 3555
Size: 5965
KpnI G_GTAC'C
Cuts at: 3290 3290
Size: 5965
MfeI C'AATT_G
Cuts at: 3564 3564
Size: 5965
MluI A'CGCG_T
Cuts at: 5841 5841
Size: 5965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2049 5079 5263 1305
Size: 744 3030 184 2007
Fragments arranged by size:
3030 2007 744 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4143
Fragments arranged by size:
4143 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2792 3923 4282 4441 518
Size: 648 1626 1131 359 159 2042
Fragments arranged by size:
2042 1626 1131 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3080 3381 4058 5206 5451 1606
Size: 1474 301 677 1148 245 2120
Fragments arranged by size:
2120 1474 1148 677 301 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4474
Fragments arranged by size:
4474 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5965
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5965
NotI GC'GGCC_GC
Cuts at: 3412 3412
Size: 5965
NspI r_CATG'y
Cuts at: 1132 1229 2085 2194 2326 2413 2443 3154 3323
Size: 97 856 109 132 87 30 711 169
Cuts at: 3323 4868 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 856 711 169 132 109 97
87 30
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5965
PciI A'CATG_T
Cuts at: 2081 3150 4864 2081
Size: 1069 1714 3182
Fragments arranged by size:
3182 1714 1069
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3140 3163 1108
Size: 2032 23 3910
Fragments arranged by size:
3910 2032 23
PfoI T'CCnGG_A
Cuts at: 2989 3193 2989
Size: 204 5761
PmeI GTTT'AAAC
Cuts at: 3269 3269
Size: 5965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4034 4066 5547 5579 4034
Size: 32 1481 32 4420
Fragments arranged by size:
4420 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3220 3220
Size: 5965
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5965
PsiI TTA'TAA
Cuts at: 3535 3535
Size: 5965
PstI C_TGCA'G
Cuts at: 830 2228 2845 3025 3317 830
Size: 1398 617 180 292 3478
Fragments arranged by size:
3478 1398 617 292 180
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5965
PvuII CAG'CTG
Cuts at: 3080 3080
Size: 5965
SacI G_AGCT'C
Cuts at: 3007 3241 3007
Size: 234 5731
SalI G'TCGA_C
Cuts at: 1936 3307 1936
Size: 1371 4594
SapI GCTCTTCn'nnn_
Cuts at: 3012 3012
Size: 5965
SbfI CC_TGCA'GG
Cuts at: 3317 3317
Size: 5965
ScaI AGT'ACT
Cuts at: 2351 4213 5925 2351
Size: 1862 1712 2391
Fragments arranged by size:
2391 1862 1712
SfcI C'TryA_G
Cuts at: 826 1045 2224 2562 2628 2841 2958 3021 3313
Size: 219 1179 338 66 213 117 63 292
Cuts at: 3313 4448 5129 5320 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1179 1135 681 338 292 219 213
191 117 66 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5965
SmaI CCC'GGG
Cuts at: 1872 3292 1872
Size: 1420 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4069 4970 5232 820
Size: 197 926 2126 901 262 1553
Fragments arranged by size:
2126 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3756 493
Size: 3263 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5965
SphI G_CATG'C
Cuts at: 1132 2194 2413 3323 1132
Size: 1062 219 910 3774
Fragments arranged by size:
3774 1062 910 219
SspI AAT'ATT
Cuts at: 5 52 2596 3889 5
Size: 47 2544 1293 2081
Fragments arranged by size:
2544 2081 1293 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5965
StyI C'CwwG_G
Cuts at: 513 1065 2004 3404 513
Size: 552 939 1400 3074
Fragments arranged by size:
3074 1400 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2497 3174 3992 4009 4162 4347 4792
Size: 1255 917 677 818 17 153 185 445
Cuts at: 4792 325
Size: 1498
Fragments arranged by size:
1498 1255 917 818 677 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2349 4211 5923
Size: 274 80 33 51 1443 372 1862 1712
Cuts at: 5923 96
Size: 138
Fragments arranged by size:
1862 1712 1443 372 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2483 2515 3138 3170 5925 5957 2483
Size: 32 623 32 2755 32 2491
Fragments arranged by size:
2755 2491 623 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5965
XbaI T'CTAG_A
Cuts at: 3301 3301
Size: 5965
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2084 2084
Size: 5965
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5965
XmaI C'CCGG_G
Cuts at: 1870 3290 1870
Size: 1420 4545
XmnI GAAnn'nnTTC
Cuts at: 2985 4094 2985
Size: 1109 4856
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3773 276
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI