Restriction map for
pFN21AB8659
(Circular) (Six-base) MAPSORT of: pFN21AB8659.seq Check: 5111 from: 1 to: 6679
pFN21AB8659 6679 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2428 4489 278
Size: 53 83 186 936 892 2061 2468
Fragments arranged by size:
2468 2061 936 892 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2341 3347 3955 4022 1937
Size: 404 1006 608 67 4594
Fragments arranged by size:
4594 1006 608 404 67
Acc65I G'GTAC_C
Cuts at: 4000 4000
Size: 6679
AclI AA'CG_TT
Cuts at: 1563 4806 5179 1563
Size: 3243 373 3063
Fragments arranged by size:
3243 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2138 3632 4742 6126 6394 1632
Size: 506 1494 1110 1384 268 1917
Fragments arranged by size:
1917 1494 1384 1110 506 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6482
AflIII A'CryG_T
Cuts at: 1203 3445 5578 6555 1203
Size: 2242 2133 977 1327
Fragments arranged by size:
2242 2133 1327 977
AgeI A'CCGG_T
Cuts at: 5510 5510
Size: 6679
AhdI GACnn_n'nnGTC
Cuts at: 5408 5408
Size: 6679
AleI CACnn'nnGTG
Cuts at: 1166 2112 1166
Size: 946 5733
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2688 1184
Size: 1504 5175
AlwNI CAG_nnn'CTG
Cuts at: 2165 2458 3035 4422 5994 2165
Size: 293 577 1387 1572 2850
Fragments arranged by size:
2850 1572 1387 577 293
ApaI G_GGCC'C
Cuts at: 2050 2050
Size: 6679
ApaLI G'TGCA_C
Cuts at: 4736 5892 4736
Size: 1156 5523
ApoI r'AATT_y
Cuts at: 1493 2482 2835 3988 4214 4366 6475 1493
Size: 989 353 1153 226 152 2109 1697
Fragments arranged by size:
2109 1697 1153 989 353 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5233 160
Size: 872 4201 1606
Fragments arranged by size:
4201 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6679
AvaI C'yCGr_G
Cuts at: 1870 1943 3854 4004 1870
Size: 73 1911 150 4545
Fragments arranged by size:
4545 1911 150 73
BamHI G'GATC_C
Cuts at: 4009 4376 4009
Size: 367 6312
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2496 4000 5455 618
Size: 325 217 627 709 1504 1455 1842
Fragments arranged by size:
1842 1504 1455 709 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2023 2050 2177 3120 3890 3998 1391
Size: 632 27 127 943 770 108 4072
Fragments arranged by size:
4072 943 770 632 127 108 27
BbeI G_GCGC'C
Cuts at: 2500 2500
Size: 6679
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2955 2982 928
Size: 971 1056 27 4625
Fragments arranged by size:
4625 1056 971 27
BbvCI CC'TCA_GC
Cuts at: 2259 2259
Size: 6679
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4870 4904 1903
Size: 34 2933 34 3678
Fragments arranged by size:
3678 2933 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3106 3539 4571 5781 3106
Size: 433 1032 1210 4004
Fragments arranged by size:
4004 1210 1032 433
BclI T'GATC_A
Cuts at: 1548 2634 3388 1548
Size: 1086 754 4839
Fragments arranged by size:
4839 1086 754
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5290 136
Size: 107 122 71 631 605 3618 1525
Fragments arranged by size:
3618 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6674 6674
Size: 6679
BlpI GC'TnA_GC
Cuts at: 2661 4096 2661
Size: 1435 5244
Bme1580I G_kGCm'C
Cuts at: 1790 2050 4740 5896 1790
Size: 260 2690 1156 2573
Fragments arranged by size:
2690 2573 1156 260
BmgBI CAC'GTC
Cuts at: 1318 2964 1318
Size: 1646 5033
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3147 4410 5368
Size: 416 53 83 399 24 1730 1263 958
Cuts at: 5368 442
Size: 1753
Fragments arranged by size:
1753 1730 1263 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2527 3134 5339 1239
Size: 1288 607 2205 2579
Fragments arranged by size:
2579 2205 1288 607
Bpu10I CC'TnA_GC
Cuts at: 2259 2259
Size: 6679
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4804 5669 5967 4804
Size: 865 298 5516
Fragments arranged by size:
5516 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2344 5342 882
Size: 617 845 2998 2219
Fragments arranged by size:
2998 2219 845 617
BsaAI yAC'GTr
Cuts at: 493 1204 4470 493
Size: 711 3266 2702
Fragments arranged by size:
3266 2702 711
BsaBI GATnn'nnATC
Cuts at: 3498 6673 3498
Size: 3175 3504
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2425 2497 4486 4868
Size: 53 83 186 936 892 72 1989 382
Cuts at: 4868 5503 275
Size: 635 1451
Fragments arranged by size:
1989 1451 936 892 635 382 186 83
72 53
BsaWI w'CCGG_w
Cuts at: 5112 5510 5784 5931 6342 5112
Size: 398 274 147 411 5449
Fragments arranged by size:
5449 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2081 2111 1178
Size: 30 165 30 678 30 5746
Fragments arranged by size:
5746 678 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2029 2303 2362 2954 2957 3642 1186
Size: 843 274 59 592 3 685 4223
Fragments arranged by size:
4223 843 685 592 274 59 3
BseYI C'CCAG_C
Cuts at: 2038 2863 5882 6378 2038
Size: 825 3019 496 2339
Fragments arranged by size:
3019 2339 825 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6106
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2028 3171 4129 4890 5918 1540
Size: 275 186 27 1143 958 761 1028 2301
Fragments arranged by size:
2301 1143 1028 958 761 275 186 27
BsiHKAI G_wGCw'C
Cuts at: 2177 2969 3120 3890 4740 4825 5896 2177
Size: 792 151 770 850 85 1071 2960
Fragments arranged by size:
2960 1071 850 792 770 151 85
BsmI GAATG_Cn'
Cuts at: 4190 4283 4190
Size: 93 6586
BsmBI CGTCTCn'nnnn_
Cuts at: 2416 2416
Size: 6679
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2050 2177 2969 3120 3890 3998
Size: 399 233 27 127 792 151 770 108
Cuts at: 3998 4740 4825 5896 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 792 770 742 399 233 151
127 108 85 27
BspEI T'CCGG_A
Cuts at: 6342 6342
Size: 6679
BspHI T'CATG_A
Cuts at: 3561 4568 6298 3561
Size: 1007 1730 3942
Fragments arranged by size:
3942 1730 1007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4034 844
Size: 3190 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4566 1394
Size: 429 2743 3507
Fragments arranged by size:
3507 2743 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5174 5348 65
Size: 1183 3926 174 1396
Fragments arranged by size:
3926 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2027 5323 5510 1700
Size: 110 217 3296 187 2869
Fragments arranged by size:
3296 2869 217 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6679
BssHII G'CGCG_C
Cuts at: 1925 3599 1925
Size: 1674 5005
BssSI C'ACGA_G
Cuts at: 4739 5751 4739
Size: 1012 5667
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2207 3881 794
Size: 1413 1674 3592
Fragments arranged by size:
3592 1674 1413
BstBI TT'CG_AA
Cuts at: 2691 5483 2691
Size: 2792 3887
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6679
BstYI r'GATC_y
Cuts at: 1972 3499 3664 4009 4376 4761 4778 6230 6316
Size: 1527 165 345 367 385 17 1452 86
Cuts at: 6316 6328 6674 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1527 1452 385 367 346 345 165
86 17 12
BstZ17I GTA'TAC
Cuts at: 2342 2342
Size: 6679
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5188
Fragments arranged by size:
5188 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5940
Fragments arranged by size:
5940 683 56
BtsI GCAGTG_nn'
Cuts at: 3879 4195 4989 5009 3879
Size: 316 794 20 5549
Fragments arranged by size:
5549 794 316 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6679
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6644
DraI TTT'AAA
Cuts at: 3983 3983
Size: 6679
DraIII CAC_nnn'GTG
Cuts at: 2888 2888
Size: 6679
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5686 809
Size: 507 4370 1802
Fragments arranged by size:
4370 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2730 4126
Size: 54 1425 50 275 18 195 705 1396
Cuts at: 4126 5015 6644 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1396 889 705 275 195 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2025 4126 1537
Size: 275 213 2101 4090
Fragments arranged by size:
4090 2101 275 213
EarI CTCTTCn'nnn_
Cuts at: 1353 2439 2760 2985 3059 3655 4609 1353
Size: 1086 321 225 74 596 954 3423
Fragments arranged by size:
3423 1086 954 596 321 225 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5266 5640 5786 387
Size: 1064 138 3677 374 146 1280
Fragments arranged by size:
3677 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2175 3118 3888 2175
Size: 943 770 4966
Fragments arranged by size:
4966 943 770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2527 3134 3632 4742 5339 6126
Size: 393 506 389 607 498 1110 597 787
Cuts at: 6126 6394 1239
Size: 268 1524
Fragments arranged by size:
1524 1110 787 607 597 506 498 393
389 268
EcoO109I rG'GnC_Cy
Cuts at: 2047 2047
Size: 6679
EcoRI G'AATT_C
Cuts at: 3988 4366 3988
Size: 378 6301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2665 2665
Size: 6679
FspI TGC'GCA
Cuts at: 5185 5185
Size: 6679
HaeII r_GCGC'y
Cuts at: 1678 2500 5826 1678
Size: 822 3326 2531
Fragments arranged by size:
3326 2531 822
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2452 2484 3385 3417 5320
Size: 32 135 32 880 32 901 32 1903
Cuts at: 5320 5352 5394 5426 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1903 901 880 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3534 3781 4023 4269 669
Size: 988 281 1596 247 242 246 3079
Fragments arranged by size:
3079 1596 988 281 247 246 242
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6679
HpaI GTT'AAC
Cuts at: 4269 4269
Size: 6679
KasI G'GCGC_C
Cuts at: 2496 2496
Size: 6679
KpnI G_GTAC'C
Cuts at: 4004 4004
Size: 6679
MfeI C'AATT_G
Cuts at: 3384 4278 3384
Size: 894 5785
MluI A'CGCG_T
Cuts at: 6555 6555
Size: 6679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3151 3539 5793 5977 1305
Size: 1846 388 2254 184 2007
Fragments arranged by size:
2254 2007 1846 388 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4857
Fragments arranged by size:
4857 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2112 3221 4637 4996 5155 518
Size: 648 946 1109 1416 359 159 2042
Fragments arranged by size:
2042 1416 1109 946 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2668 3372 4095 4772 5920 6165 1606
Size: 465 597 704 723 677 1148 245 2120
Fragments arranged by size:
2120 1148 723 704 677 597 465 245
NarI GG'CG_CC
Cuts at: 2497 2497
Size: 6679
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5188
Fragments arranged by size:
5188 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6679
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6679
NotI GC'GGCC_GC
Cuts at: 4126 4126
Size: 6679
NspI r_CATG'y
Cuts at: 1132 1229 3449 3553 4037 5582 1132
Size: 97 2220 104 484 1545 2229
Fragments arranged by size:
2229 2220 1545 484 104 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6679
PciI A'CATG_T
Cuts at: 3445 5578 3445
Size: 2133 4546
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6679
PfoI T'CCnGG_A
Cuts at: 3655 3655
Size: 6679
PmeI GTTT'AAAC
Cuts at: 3983 3983
Size: 6679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3390 3422 4748 4780 6261 6293 3390
Size: 32 1326 32 1481 32 3776
Fragments arranged by size:
3776 1481 1326 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2278 1613
Size: 665 6014
PsiI TTA'TAA
Cuts at: 4249 4249
Size: 6679
PspOMI G'GGCC_C
Cuts at: 2046 2046
Size: 6679
PstI C_TGCA'G
Cuts at: 830 2203 3002 3031 4031 830
Size: 1373 799 29 1000 3478
Fragments arranged by size:
3478 1373 1000 799 29
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6679
SacI G_AGCT'C
Cuts at: 2177 3120 3890 2177
Size: 943 770 4966
Fragments arranged by size:
4966 943 770
SalI G'TCGA_C
Cuts at: 1936 4021 1936
Size: 2085 4594
SbfI CC_TGCA'GG
Cuts at: 4031 4031
Size: 6679
ScaI AGT'ACT
Cuts at: 4927 6639 4927
Size: 1712 4967
SexAI A'CCwGG_T
Cuts at: 3256 3256
Size: 6679
SfcI C'TryA_G
Cuts at: 826 1045 2199 2998 3027 4027 5162 5843 6034
Size: 219 1154 799 29 1000 1135 681 191
Cuts at: 6034 826
Size: 1471
Fragments arranged by size:
1471 1154 1135 1000 799 681 219 191
29
SfoI GGC'GCC
Cuts at: 2498 2498
Size: 6679
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6679
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6679
SmaI CCC'GGG
Cuts at: 1872 3856 4006 1872
Size: 1984 150 4545
Fragments arranged by size:
4545 1984 150
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4783 5684 5946 820
Size: 197 926 2840 901 262 1553
Fragments arranged by size:
2840 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4470 493
Size: 3977 2702
SpeI A'CTAG_T
Cuts at: 152 3421 152
Size: 3269 3410
SphI G_CATG'C
Cuts at: 1132 4037 1132
Size: 2905 3774
SspI AAT'ATT
Cuts at: 5 52 2242 3436 4603 5
Size: 47 2190 1194 1167 2081
Fragments arranged by size:
2190 2081 1194 1167 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6679
StyI C'CwwG_G
Cuts at: 513 1065 2004 2985 3351 4118 513
Size: 552 939 981 366 767 3074
Fragments arranged by size:
3074 981 939 767 552 366
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4706 4723 4876 5061 5506 325
Size: 1255 3126 17 153 185 445 1498
Fragments arranged by size:
3126 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2685 3639 4925
Size: 274 80 33 51 1443 708 954 1286
Cuts at: 4925 6637 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1286 954 708 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2081 2113 2834 2866 6639 6671 2081
Size: 32 721 32 3773 32 2089
Fragments arranged by size:
3773 2089 721 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3620 1316
Size: 2304 4375
XbaI T'CTAG_A
Cuts at: 2413 4015 2413
Size: 1602 5077
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3653 3653
Size: 6679
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6679
XmaI C'CCGG_G
Cuts at: 1870 3854 4004 1870
Size: 1984 150 4545
Fragments arranged by size:
4545 1984 150
XmnI GAAnn'nnTTC
Cuts at: 4808 4808
Size: 6679
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2426 4487 276
Size: 53 83 186 936 892 2061 2468
Fragments arranged by size:
2468 2061 936 892 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI SacI SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI Bsu36I EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SfiI SrfI SwaI