Restriction map for
pFN21AB8657
(Circular) (Six-base) MAPSORT of: pFN21AB8657.seq Check: 5410 from: 1 to: 6067
pFN21AB8657 6067 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3877 278
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3410 1937
Size: 1473 4594
Acc65I G'GTAC_C
Cuts at: 3060 3388 3060
Size: 328 5739
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2755 4130 5514 5782 1632
Size: 1123 1375 1384 268 1917
Fragments arranged by size:
1917 1384 1375 1123 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5870
AflIII A'CryG_T
Cuts at: 1203 3069 4966 5943 1203
Size: 1866 1897 977 1327
Fragments arranged by size:
1897 1866 1327 977
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 2081 1166
Size: 915 5152
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2771 2803 2771
Size: 32 6035
AlwNI CAG_nnn'CTG
Cuts at: 2389 3810 5382 2389
Size: 1421 1572 3074
Fragments arranged by size:
3074 1572 1421
ApaLI G'TGCA_C
Cuts at: 2819 4124 5280 2819
Size: 1305 1156 3606
Fragments arranged by size:
3606 1305 1156
ApoI r'AATT_y
Cuts at: 1493 2165 3376 3602 3754 5863 1493
Size: 672 1211 226 152 2109 1697
Fragments arranged by size:
2109 1697 1211 672 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2574 4621 160
Size: 872 1542 2047 1606
Fragments arranged by size:
2047 1606 1542 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 2834 3392 1870
Size: 73 891 558 4545
Fragments arranged by size:
4545 891 558 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3216 3249 3216
Size: 33 6034
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 3047 3060 3287 3388
Size: 325 217 627 249 1011 13 227 101
Cuts at: 3388 4843 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1011 627 325 249 227 217
101 13
BanII G_rGCy'C
Cuts at: 1391 2868 3386 1391
Size: 1477 518 4072
Fragments arranged by size:
4072 1477 518
BbeI G_GCGC'C
Cuts at: 2040 3291 2040
Size: 1251 4816
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2554 928
Size: 971 655 4441
Fragments arranged by size:
4441 971 655
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2944 2978 3257 3291 4258 4292 1903
Size: 34 1007 34 279 34 967 34 3678
Fragments arranged by size:
3678 1007 967 279 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3959 5169 3959
Size: 1210 4857
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6067
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4678 136
Size: 107 122 71 631 605 3006 1525
Fragments arranged by size:
3006 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2202 2331 3156 6062 2202
Size: 129 825 2906 2207
Fragments arranged by size:
2906 2207 825 129
BlpI GC'TnA_GC
Cuts at: 3484 3484
Size: 6067
Bme1580I G_kGCm'C
Cuts at: 1790 2823 3109 4128 5284 1790
Size: 1033 286 1019 1156 2573
Fragments arranged by size:
2573 1156 1033 1019 286
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3798 4756 442
Size: 416 53 83 399 24 2381 958 1753
Fragments arranged by size:
2381 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3014 1056
Size: 1958 4109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2239 2423 2785 3317 4727 1239
Size: 1000 184 362 532 1410 2579
Fragments arranged by size:
2579 1410 1000 532 362 184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4192 5057 5355 4192
Size: 865 298 4904
Fragments arranged by size:
4904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2807 4730 882
Size: 617 1308 1923 2219
Fragments arranged by size:
2219 1923 1308 617
BsaAI yAC'GTr
Cuts at: 493 1204 3232 3858 493
Size: 711 2028 626 2702
Fragments arranged by size:
2702 2028 711 626
BsaBI GATnn'nnATC
Cuts at: 6061 6061
Size: 6067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 2465 3288 3874
Size: 53 83 186 936 504 428 823 586
Cuts at: 3874 4256 4891 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 823 635 586 504 428 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2156 4500 4898 5172 5319 5730 2156
Size: 2344 398 274 147 411 2493
Fragments arranged by size:
2493 2344 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2333 2363 2399 2429 1178
Size: 30 165 30 930 30 36 30 4816
Fragments arranged by size:
4816 930 165 36 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 2748 2876 1186
Size: 1234 328 128 4377
Fragments arranged by size:
4377 1234 328 128
BseYI C'CCAG_C
Cuts at: 5270 5766 5270
Size: 496 5571
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2576 1163
Size: 573 840 4654
Fragments arranged by size:
4654 840 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3517 4278 5306 1540
Size: 275 186 1516 761 1028 2301
Fragments arranged by size:
2301 1516 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2823 2901 3342 4128 4213 5284 2823
Size: 78 441 786 85 1071 3606
Fragments arranged by size:
3606 1071 786 441 85 78
BsmI GAATG_Cn'
Cuts at: 2216 3578 3671 2216
Size: 1362 93 4612
Fragments arranged by size:
4612 1362 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2456 2456
Size: 6067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2823 2868 2901 3109 3342 3386 4128
Size: 399 1033 45 33 208 233 44 742
Cuts at: 4128 4213 5284 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1033 742 399 233 208 85
45 44 33
BspEI T'CCGG_A
Cuts at: 5730 5730
Size: 6067
BspHI T'CATG_A
Cuts at: 3956 5686 3956
Size: 1730 4337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2548 2928 3422 844
Size: 1704 380 494 3489
Fragments arranged by size:
3489 1704 494 380
BsrBI CCG'CTC
Cuts at: 1394 1823 2025 3954 1394
Size: 429 202 1929 3507
Fragments arranged by size:
3507 1929 429 202
BsrDI GCAATG_nn'
Cuts at: 65 1248 4562 4736 65
Size: 1183 3314 174 1396
Fragments arranged by size:
3314 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 2039 3123 4711 4898 1700
Size: 110 223 6 1084 1588 187 2869
Fragments arranged by size:
2869 1588 1084 223 187 110 6
BsrGI T'GTAC_A
Cuts at: 96 2415 3066 3072 96
Size: 2319 651 6 3091
Fragments arranged by size:
3091 2319 651 6
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6067
BssSI C'ACGA_G
Cuts at: 4127 5139 4127
Size: 1012 5055
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6067
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6067
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6067
BstYI r'GATC_y
Cuts at: 1972 2202 2331 3027 3156 3397 3764 4149 4166
Size: 230 129 696 129 241 367 385 17
Cuts at: 4166 5618 5704 5716 6062 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 696 385 367 346 241 230
129 129 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2658 2967 3032 513
Size: 552 1593 309 65 3548
Fragments arranged by size:
3548 1593 552 309 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5328
Fragments arranged by size:
5328 683 56
BtsI GCAGTG_nn'
Cuts at: 3583 4377 4397 3583
Size: 794 20 5253
Fragments arranged by size:
5253 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 3371 3371
Size: 6067
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2041 2113 2268
Size: 54 1425 50 275 18 211 72 155
Cuts at: 2268 2989 3514 4403 6032 8
Size: 721 525 889 1629 43
Fragments arranged by size:
1629 1425 889 721 525 275 211 155
72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3514 1537
Size: 275 1702 4090
Fragments arranged by size:
4090 1702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2057 3997 1353
Size: 704 1940 3423
Fragments arranged by size:
3423 1940 704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2785 2963 4654 5028 5174 387
Size: 1064 138 1196 178 1691 374 146 1280
Fragments arranged by size:
1691 1280 1196 1064 374 178 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2239 2423 2755 2785 3317 4130 4727
Size: 393 607 184 332 30 532 813 597
Cuts at: 4727 5514 5782 1239
Size: 787 268 1524
Fragments arranged by size:
1524 813 787 607 597 532 393 332
268 184 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3326 3326
Size: 6067
EcoRI G'AATT_C
Cuts at: 3376 3754 3376
Size: 378 5689
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 2040 3291 3315 5214 1678
Size: 362 1251 24 1899 2531
Fragments arranged by size:
2531 1899 1251 362 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2587 2619 2700 2732 3155
Size: 32 135 32 1015 32 81 32 423
Cuts at: 3155 3187 4708 4740 4782 4814 1373
Size: 32 1521 32 42 32 2626
Fragments arranged by size:
2626 1521 1015 423 135 81 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3411 3657 669
Size: 988 281 1473 246 3079
Fragments arranged by size:
3079 1473 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6067
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KasI G'GCGC_C
Cuts at: 2036 3287 2036
Size: 1251 4816
KpnI G_GTAC'C
Cuts at: 3064 3392 3064
Size: 328 5739
MfeI C'AATT_G
Cuts at: 3666 3666
Size: 6067
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2376 5181 5365 1305
Size: 1071 2805 184 2007
Fragments arranged by size:
2805 2007 1071 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2270 10
Size: 54 1425 343 438 3807
Fragments arranged by size:
3807 1425 438 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2081 2441 4025 4384 4543 518
Size: 648 915 360 1584 359 159 2042
Fragments arranged by size:
2042 1584 915 648 360 359 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2105 3034 3212 3483 4160 5308 5553
Size: 441 58 929 178 271 677 1148 245
Cuts at: 5553 1606
Size: 2120
Fragments arranged by size:
2120 1148 929 677 441 271 245 178
58
NaeI GCC'GGC
Cuts at: 2035 2041 2035
Size: 6 6061
NarI GG'CG_CC
Cuts at: 2037 3288 2037
Size: 1251 4816
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5515
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6067
NgoMIV G'CCGG_C
Cuts at: 2033 2039 2033
Size: 6 6061
NheI G'CTAG_C
Cuts at: 1052 3010 1052
Size: 1958 4109
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 2788 3073 3425 4970 1132
Size: 97 1559 285 352 1545 2229
Fragments arranged by size:
2229 1559 1545 352 285 97
PasI CC'CwG_GG
Cuts at: 1348 2531 1348
Size: 1183 4884
PciI A'CATG_T
Cuts at: 3069 4966 3069
Size: 1897 4170
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2874 1108
Size: 1766 4301
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2234 2266 4136 4168 5649 5681 2234
Size: 32 1870 32 1481 32 2620
Fragments arranged by size:
2620 1870 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3135 1613
Size: 1522 4545
PsiI TTA'TAA
Cuts at: 2163 3637 2163
Size: 1474 4593
PstI C_TGCA'G
Cuts at: 830 2482 3217 3419 830
Size: 1652 735 202 3478
Fragments arranged by size:
3478 1652 735 202
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
PvuII CAG'CTG
Cuts at: 2047 2105 3212 2047
Size: 58 1107 4902
Fragments arranged by size:
4902 1107 58
RsrII CG'GwC_CG
Cuts at: 2971 2971
Size: 6067
SacII CC_GC'GG
Cuts at: 3035 3035
Size: 6067
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SapI GCTCTTCn'nnn_
Cuts at: 2057 2057
Size: 6067
SbfI CC_TGCA'GG
Cuts at: 2482 3419 2482
Size: 937 5130
ScaI AGT'ACT
Cuts at: 4315 6027 4315
Size: 1712 4355
SfcI C'TryA_G
Cuts at: 826 1045 2341 2478 3213 3415 4550 5231 5422
Size: 219 1296 137 735 202 1135 681 191
Cuts at: 5422 826
Size: 1471
Fragments arranged by size:
1471 1296 1135 735 681 219 202 191
137
SfoI GGC'GCC
Cuts at: 2038 3289 2038
Size: 1251 4816
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 3394 1872
Size: 1522 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2834 4171 5072 5334 820
Size: 197 926 891 1337 901 262 1553
Fragments arranged by size:
1553 1337 926 901 891 262 197
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3425 1132
Size: 2293 3774
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 2484 1517
Size: 967 5100
StyI C'CwwG_G
Cuts at: 513 1065 2291 3506 513
Size: 552 1226 1215 3074
Fragments arranged by size:
3074 1226 1215 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3035 3263 3272 4094 4111 4264 4449
Size: 1255 1455 228 9 822 17 153 185
Cuts at: 4449 4894 325
Size: 445 1498
Fragments arranged by size:
1498 1455 1255 822 445 228 185 153
17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2415 3066 3072
Size: 274 80 33 51 1443 438 651 6
Cuts at: 3072 4313 6025 96
Size: 1241 1712 138
Fragments arranged by size:
1712 1443 1241 651 438 274 138 80
51 33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2216 2248 6027 6059 2216
Size: 32 3779 32 2224
Fragments arranged by size:
3779 2224 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6067
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2263 2359 3157 2263
Size: 96 798 5173
Fragments arranged by size:
5173 798 96
XhoI C'TCGA_G
Cuts at: 1943 2834 1943
Size: 891 5176
XmaI C'CCGG_G
Cuts at: 1870 3392 1870
Size: 1522 4545
XmnI GAAnn'nnTTC
Cuts at: 4196 4196
Size: 6067
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3875 276
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI SacI SanDI SexAI SfiI
SrfI SwaI