Restriction map for
pFN21AB8656
(Circular) (Six-base) MAPSORT of: pFN21AB8656.seq Check: 5184 from: 1 to: 6406
pFN21AB8656 6406 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2698 3147 2698
Size: 449 5957
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4216 278
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2106 2389 3159 3749 1937
Size: 169 283 770 590 4594
Fragments arranged by size:
4594 770 590 283 169
Acc65I G'GTAC_C
Cuts at: 3727 3727
Size: 6406
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2352 2903 2978 4469 5853 6121 1632
Size: 720 551 75 1491 1384 268 1917
Fragments arranged by size:
1917 1491 1384 720 551 268 75
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6209
AflIII A'CryG_T
Cuts at: 1203 2310 5305 6282 1203
Size: 1107 2995 977 1327
Fragments arranged by size:
2995 1327 1107 977
AgeI A'CCGG_T
Cuts at: 2541 5237 2541
Size: 2696 3710
AhdI GACnn_n'nnGTC
Cuts at: 5135 5135
Size: 6406
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2125 1184
Size: 941 5465
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2423 2455 2423
Size: 32 6374
AlwNI CAG_nnn'CTG
Cuts at: 4149 5721 4149
Size: 1572 4834
ApaI G_GGCC'C
Cuts at: 2478 2478
Size: 6406
ApaLI G'TGCA_C
Cuts at: 4463 5619 4463
Size: 1156 5250
ApoI r'AATT_y
Cuts at: 1493 2533 3039 3244 3377 3715 3941 4093 6202
Size: 1040 506 205 133 338 226 152 2109
Cuts at: 6202 1493
Size: 1697
Fragments arranged by size:
2109 1697 1040 506 338 226 205 152
133
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 3731 1870
Size: 73 1788 4545
Fragments arranged by size:
4545 1788 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2990 3023 2990
Size: 33 6373
BamHI G'GATC_C
Cuts at: 3736 4103 3736
Size: 367 6039
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3337 3727 5182 618
Size: 325 217 627 1550 390 1455 1842
Fragments arranged by size:
1842 1550 1455 627 390 325 217
BanII G_rGCy'C
Cuts at: 1391 2283 2478 2492 2500 3725 1391
Size: 892 195 14 8 1225 4072
Fragments arranged by size:
4072 1225 892 195 14 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5435
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4597 4631 1903
Size: 34 2660 34 3678
Fragments arranged by size:
3678 2660 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4298 5508 4298
Size: 1210 5196
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5017 136
Size: 107 122 71 631 605 3345 1525
Fragments arranged by size:
3345 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6401 6401
Size: 6406
BlpI GC'TnA_GC
Cuts at: 3823 3823
Size: 6406
Bme1580I G_kGCm'C
Cuts at: 1790 2478 4467 5623 1790
Size: 688 1989 1156 2573
Fragments arranged by size:
2573 1989 1156 688
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2565 4137 5095
Size: 416 53 83 399 24 1148 1572 958
Cuts at: 5095 442
Size: 1753
Fragments arranged by size:
1753 1572 1148 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6406
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2189 2449 3202 2189
Size: 260 753 5393
Fragments arranged by size:
5393 753 260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2419 2514 3601 5066 1239
Size: 1180 95 1087 1465 2579
Fragments arranged by size:
2579 1465 1180 1087 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2101 4531 5396 5694 2101
Size: 2430 865 298 2813
Fragments arranged by size:
2813 2430 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5069 882
Size: 617 3570 2219
Fragments arranged by size:
3570 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4197 493
Size: 711 2993 2702
Fragments arranged by size:
2993 2702 711
BsaBI GATnn'nnATC
Cuts at: 2338 3474 6400 2338
Size: 1136 2926 2344
Fragments arranged by size:
2926 2344 1136
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4213 4595 5230 275
Size: 53 83 186 936 2680 382 635 1451
Fragments arranged by size:
2680 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2541 4839 5237 5511 5658 6069 2541
Size: 2298 398 274 147 411 2878
Fragments arranged by size:
2878 2298 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6181
Fragments arranged by size:
6181 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2622 1186
Size: 1436 4970
BseYI C'CCAG_C
Cuts at: 3239 5609 6105 3239
Size: 2370 496 3540
Fragments arranged by size:
3540 2370 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3154 1163
Size: 573 1418 4415
Fragments arranged by size:
4415 1418 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3856 4617 5645 1540
Size: 275 186 1855 761 1028 2301
Fragments arranged by size:
2301 1855 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2087 2492 4467 4552 5623 2087
Size: 405 1975 85 1071 2870
Fragments arranged by size:
2870 1975 1071 405 85
BsmI GAATG_Cn'
Cuts at: 2923 3406 3917 4010 2923
Size: 483 511 93 5319
Fragments arranged by size:
5319 511 483 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2283 2478 2492 2500 3725 4467
Size: 399 297 196 195 14 8 1225 742
Cuts at: 4467 4552 5623 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1225 1071 742 399 297 196 195
85 14 8
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 3640 4295 6025 3640
Size: 655 1730 4021
Fragments arranged by size:
4021 1730 655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2698 3147 3761 844
Size: 1854 449 614 3489
Fragments arranged by size:
3489 1854 614 449
BsrBI CCG'CTC
Cuts at: 1394 1823 4293 1394
Size: 429 2470 3507
Fragments arranged by size:
3507 2470 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2232 2946 4901 5075 65
Size: 1183 984 714 1955 174 1396
Fragments arranged by size:
1955 1396 1183 984 714 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2541 5050 5237 1700
Size: 110 731 2509 187 2869
Fragments arranged by size:
2869 2509 731 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6406
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6406
BssSI C'ACGA_G
Cuts at: 4466 5478 4466
Size: 1012 5394
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6406
BstBI TT'CG_AA
Cuts at: 2561 5210 2561
Size: 2649 3757
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6406
BstYI r'GATC_y
Cuts at: 1972 2578 3736 4103 4488 4505 5957 6043 6055
Size: 606 1158 367 385 17 1452 86 12
Cuts at: 6055 6401 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1158 606 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2284 2284
Size: 6406
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4915
Fragments arranged by size:
4915 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5667
Fragments arranged by size:
5667 683 56
BtsI GCAGTG_nn'
Cuts at: 3922 4716 4736 3922
Size: 794 20 5592
Fragments arranged by size:
5592 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6371
DraI TTT'AAA
Cuts at: 2071 2643 2758 3710 2071
Size: 572 115 952 4767
Fragments arranged by size:
4767 952 572 115
DraIII CAC_nnn'GTG
Cuts at: 2195 2573 2195
Size: 378 6028
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5413 809
Size: 507 4097 1802
Fragments arranged by size:
4097 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3853 4742 6371
Size: 54 1425 50 275 18 2023 889 1629
Cuts at: 6371 8
Size: 43
Fragments arranged by size:
2023 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3853 1537
Size: 275 2041 4090
Fragments arranged by size:
4090 2041 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2158 2371 2922 4336 1353
Size: 805 213 551 1414 3423
Fragments arranged by size:
3423 1414 805 551 213
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4993 5367 5513 387
Size: 1064 138 3404 374 146 1280
Fragments arranged by size:
3404 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2490 2490
Size: 6406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2352 2419 2514 2903 2978 3601 4469
Size: 393 720 67 95 389 75 623 868
Cuts at: 4469 5066 5853 6121 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 868 787 720 623 597 393 389
268 95 75 67
EcoNI CCTnn'n_nnAGG
Cuts at: 2347 2874 2932 2347
Size: 527 58 5821
Fragments arranged by size:
5821 527 58
EcoO109I rG'GnC_Cy
Cuts at: 2475 2475
Size: 6406
EcoRI G'AATT_C
Cuts at: 3244 3715 4093 3244
Size: 471 378 5557
Fragments arranged by size:
5557 471 378
FspI TGC'GCA
Cuts at: 4912 4912
Size: 6406
HaeII r_GCGC'y
Cuts at: 1678 5553 1678
Size: 3875 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5047 5079 5121 5153 1373
Size: 32 135 32 3475 32 42 32 2626
Fragments arranged by size:
3475 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3302 3750 3996 669
Size: 988 281 1364 448 246 3079
Fragments arranged by size:
3079 1364 988 448 281 246
HindIII A'AGCT_T
Cuts at: 748 2752 748
Size: 2004 4402
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KpnI G_GTAC'C
Cuts at: 3731 3731
Size: 6406
MfeI C'AATT_G
Cuts at: 2618 4005 2618
Size: 1387 5019
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5520 5704 1305
Size: 4215 184 2007
Fragments arranged by size:
4215 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4584
Fragments arranged by size:
4584 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3674 4364 4723 4882 518
Size: 648 2508 690 359 159 2042
Fragments arranged by size:
2508 2042 690 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2777 3822 4499 5647 5892 1606
Size: 1171 1045 677 1148 245 2120
Fragments arranged by size:
2120 1171 1148 1045 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4915
Fragments arranged by size:
4915 939 552
NdeI CA'TA_TG
Cuts at: 387 2839 387
Size: 2452 3954
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6406
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 2314 3149 3764 5309 1132
Size: 97 1085 835 615 1545 2229
Fragments arranged by size:
2229 1545 1085 835 615 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6406
PciI A'CATG_T
Cuts at: 2310 5305 2310
Size: 2995 3411
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6406
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 5988 6020 4475
Size: 32 1481 32 4861
Fragments arranged by size:
4861 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6406
PsiI TTA'TAA
Cuts at: 2241 3396 3422 3976 2241
Size: 1155 26 554 4671
Fragments arranged by size:
4671 1155 554 26
PspOMI G'GGCC_C
Cuts at: 2474 2474
Size: 6406
PstI C_TGCA'G
Cuts at: 830 2104 3758 830
Size: 1274 1654 3478
Fragments arranged by size:
3478 1654 1274
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6406
PvuII CAG'CTG
Cuts at: 2777 2777
Size: 6406
SacI G_AGCT'C
Cuts at: 2492 2492
Size: 6406
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SapI GCTCTTCn'nnn_
Cuts at: 2922 2922
Size: 6406
SbfI CC_TGCA'GG
Cuts at: 3758 3758
Size: 6406
ScaI AGT'ACT
Cuts at: 4654 6366 4654
Size: 1712 4694
SfcI C'TryA_G
Cuts at: 826 1045 2100 3754 4889 5570 5761 826
Size: 219 1055 1654 1135 681 191 1471
Fragments arranged by size:
1654 1471 1135 1055 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6406
SmaI CCC'GGG
Cuts at: 1872 3733 1872
Size: 1861 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2080 4510 5411 5673 820
Size: 197 926 137 2430 901 262 1553
Fragments arranged by size:
2430 1553 926 901 262 197 137
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 2356 152
Size: 2204 4202
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6406
StyI C'CwwG_G
Cuts at: 513 1065 2004 2933 3845 513
Size: 552 939 929 912 3074
Fragments arranged by size:
3074 939 929 912 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2584 4433 4450 4603 4788 5233 325
Size: 1255 1004 1849 17 153 185 445 1498
Fragments arranged by size:
1849 1498 1255 1004 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4652 6364 96
Size: 274 80 33 51 1443 2675 1712 138
Fragments arranged by size:
2675 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2404 2436 6366 6398 2404
Size: 32 3930 32 2412
Fragments arranged by size:
3930 2412 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6406
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 3731 1870
Size: 1861 4545
XmnI GAAnn'nnTTC
Cuts at: 4535 4535
Size: 6406
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4214 276
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI