Restriction map for
pFN21AB8654
(Circular) (Six-base) MAPSORT of: pFN21AB8654.seq Check: 8979 from: 1 to: 7291
pFN21AB8654 7291 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2302 3335 4322 2302
Size: 1033 987 5271
Fragments arranged by size:
5271 1033 987
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2171 5101 278
Size: 53 83 186 936 635 2930 2468
Fragments arranged by size:
2930 2468 936 635 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3795 4634 1937
Size: 1858 839 4594
Fragments arranged by size:
4594 1858 839
Acc65I G'GTAC_C
Cuts at: 4612 4612
Size: 7291
AclI AA'CG_TT
Cuts at: 1563 5418 5791 1563
Size: 3855 373 3063
Fragments arranged by size:
3855 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3074 3286 4463 5354 6738 7006 1632
Size: 1442 212 1177 891 1384 268 1917
Fragments arranged by size:
1917 1442 1384 1177 891 268 212
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7094
AflIII A'CryG_T
Cuts at: 1203 3216 3765 6190 7167 1203
Size: 2013 549 2425 977 1327
Fragments arranged by size:
2425 2013 1327 977 549
AgeI A'CCGG_T
Cuts at: 6122 6122
Size: 7291
AhdI GACnn_n'nnGTC
Cuts at: 4564 6020 4564
Size: 1456 5835
AleI CACnn'nnGTG
Cuts at: 1166 4476 1166
Size: 3310 3981
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3299 3566 1184
Size: 2115 267 4909
Fragments arranged by size:
4909 2115 267
AlwNI CAG_nnn'CTG
Cuts at: 2328 2554 3748 4323 4432 5034 6606 2328
Size: 226 1194 575 109 602 1572 3013
Fragments arranged by size:
3013 1572 1194 602 575 226 109
ApaI G_GGCC'C
Cuts at: 3315 4229 3315
Size: 914 6377
ApaLI G'TGCA_C
Cuts at: 3638 5348 6504 3638
Size: 1710 1156 4425
Fragments arranged by size:
4425 1710 1156
ApoI r'AATT_y
Cuts at: 1493 4600 4826 4978 7087 1493
Size: 3107 226 152 2109 1697
Fragments arranged by size:
3107 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5845 160
Size: 872 4813 1606
Fragments arranged by size:
4813 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7291
AvaI C'yCGr_G
Cuts at: 1870 1943 3314 4044 4222 4375 4616 1870
Size: 73 1371 730 178 153 241 4545
Fragments arranged by size:
4545 1371 730 241 178 153 73
AvrII C'CTAG_G
Cuts at: 2503 2503
Size: 7291
BamHI G'GATC_C
Cuts at: 4621 4988 4621
Size: 367 6924
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3600 3971 4612 6067 618
Size: 325 217 627 1813 371 641 1455 1842
Fragments arranged by size:
1842 1813 1455 641 627 371 325 217
BanII G_rGCy'C
Cuts at: 1391 3062 3315 3321 3501 3996 4229 4610 1391
Size: 1671 253 6 180 495 233 381 4072
Fragments arranged by size:
4072 1671 495 381 253 233 180 6
BbeI G_GCGC'C
Cuts at: 3604 3604
Size: 7291
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 2444 3344 4414 928
Size: 971 116 429 900 1070 3805
Fragments arranged by size:
3805 1070 971 900 429 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3785 3819 5482 5516 1903
Size: 34 1848 34 1663 34 3678
Fragments arranged by size:
3678 1848 1663 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5183 6393 5183
Size: 1210 6081
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7291
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3197 5902 136
Size: 107 122 71 631 605 1525 2705 1525
Fragments arranged by size:
2705 1525 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2762 7286 2762
Size: 4524 2767
BlpI GC'TnA_GC
Cuts at: 2979 4157 4708 2979
Size: 1178 551 5562
Fragments arranged by size:
5562 1178 551
Bme1580I G_kGCm'C
Cuts at: 1790 3315 3390 3642 4070 4229 5352 6508 1790
Size: 1525 75 252 428 159 1123 1156 2573
Fragments arranged by size:
2573 1525 1156 1123 428 252 159 75
BmgBI CAC'GTC
Cuts at: 1318 3766 1318
Size: 2448 4843
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2139 2734 2788
Size: 416 53 83 399 24 722 595 54
Cuts at: 2788 3100 5022 5980 442
Size: 312 1922 958 1753
Fragments arranged by size:
1922 1753 958 722 595 416 399 312
83 54 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7291
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2548 2548
Size: 7291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2861 3413 3476 5951 1239
Size: 1622 552 63 2475 2579
Fragments arranged by size:
2579 2475 1622 552 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3593 5416 6281 6579 3593
Size: 1823 865 298 4305
Fragments arranged by size:
4305 1823 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2711 3017 3941 5954 882
Size: 617 1212 306 924 2013 2219
Fragments arranged by size:
2219 2013 1212 924 617 306
BsaAI yAC'GTr
Cuts at: 493 1204 2185 5082 493
Size: 711 981 2897 2702
Fragments arranged by size:
2897 2702 981 711
BsaBI GATnn'nnATC
Cuts at: 7285 7285
Size: 7291
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2168 3153 3601 5098
Size: 53 83 186 936 635 985 448 1497
Cuts at: 5098 5480 6115 275
Size: 382 635 1451
Fragments arranged by size:
1497 1451 985 936 635 635 448 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5724 6122 6396 6543 6954 5724
Size: 398 274 147 411 6061
Fragments arranged by size:
6061 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7066
Fragments arranged by size:
7066 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3410 1186
Size: 2224 5067
BseYI C'CCAG_C
Cuts at: 4165 6494 6990 4165
Size: 2329 496 4466
Fragments arranged by size:
4466 2329 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3711 3911 1163
Size: 573 1975 200 4543
Fragments arranged by size:
4543 1975 573 200
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4741 5502 6530 1540
Size: 275 186 2740 761 1028 2301
Fragments arranged by size:
2740 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2019 3293 3321 3501 3642 3675 3996 5352 5437
Size: 1274 28 180 141 33 321 1356 85
Cuts at: 5437 6508 2019
Size: 1071 2802
Fragments arranged by size:
2802 1356 1274 1071 321 180 141 85
33 28
BsmI GAATG_Cn'
Cuts at: 2576 4802 4895 2576
Size: 2226 93 4972
Fragments arranged by size:
4972 2226 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4379 4379
Size: 7291
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 3062 3293 3315 3321 3390 3501
Size: 399 229 1043 231 22 6 69 111
Cuts at: 3501 3642 3675 3996 4070 4229 4610 5352 5437
Size: 141 33 321 74 159 381 742 85
Cuts at: 5437 6508 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1043 742 399 381 321 231
229 159 141 111 85 74 69 33
22 6
BspEI T'CCGG_A
Cuts at: 6954 6954
Size: 7291
BspHI T'CATG_A
Cuts at: 2412 3894 5180 6910 2412
Size: 1482 1286 1730 2793
Fragments arranged by size:
2793 1730 1482 1286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2302 2568 3291 3335 4322 4646 844
Size: 1458 266 723 44 987 324 3489
Fragments arranged by size:
3489 1458 987 723 324 266 44
BsrBI CCG'CTC
Cuts at: 1394 1823 2983 3597 4197 5178 1394
Size: 429 1160 614 600 981 3507
Fragments arranged by size:
3507 1160 981 614 600 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3801 5786 5960 65
Size: 1183 2553 1985 174 1396
Fragments arranged by size:
2553 1985 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3155 3603 5935 6122 1700
Size: 110 1345 448 2332 187 2869
Fragments arranged by size:
2869 2332 1345 448 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7291
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7291
BssSI C'ACGA_G
Cuts at: 2120 5351 6363 2120
Size: 3231 1012 3048
Fragments arranged by size:
3231 3048 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2328 2968 3385 4119 4254 794
Size: 1534 640 417 734 135 3831
Fragments arranged by size:
3831 1534 734 640 417 135
BstBI TT'CG_AA
Cuts at: 6095 6095
Size: 7291
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7291
BstYI r'GATC_y
Cuts at: 1972 2762 4621 4988 5373 5390 6842 6928 6940
Size: 790 1859 367 385 17 1452 86 12
Cuts at: 6940 7286 1972
Size: 346 1977
Fragments arranged by size:
1977 1859 1452 790 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2262 2425 2493 3014 3137 3258 4474
Size: 552 1197 163 68 521 123 121 1216
Cuts at: 4474 513
Size: 3330
Fragments arranged by size:
3330 1216 1197 552 521 163 123 121
68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3238 3957 4395 487
Size: 683 56 2012 719 438 3383
Fragments arranged by size:
3383 2012 719 683 438 56
BtsI GCAGTG_nn'
Cuts at: 3694 3699 3928 3937 4270 4324 4807 5601 5621
Size: 5 229 9 333 54 483 794 20
Cuts at: 5621 3694
Size: 5364
Fragments arranged by size:
5364 794 483 333 229 54 20 9
5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7291
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7256
DraI TTT'AAA
Cuts at: 2826 4595 2826
Size: 1769 5522
DraIII CAC_nnn'GTG
Cuts at: 3875 4294 3875
Size: 419 6872
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6298 809
Size: 507 4982 1802
Fragments arranged by size:
4982 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 2931 3849
Size: 54 1425 50 275 18 313 788 918
Cuts at: 3849 4174 4575 4738 5627 7256 8
Size: 325 401 163 889 1629 43
Fragments arranged by size:
1629 1425 918 889 788 401 325 313
275 163 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4738 1537
Size: 275 2926 4090
Fragments arranged by size:
4090 2926 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2944 3326 5221 1353
Size: 1591 382 1895 3423
Fragments arranged by size:
3423 1895 1591 382
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 5878 6252 6398 387
Size: 1064 138 2705 1584 374 146 1280
Fragments arranged by size:
2705 1584 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3319 3499 3994 3319
Size: 180 495 6616
Fragments arranged by size:
6616 495 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2861 3074 3286 3413 3476 4463 5354
Size: 393 1229 213 212 127 63 987 891
Cuts at: 5354 5951 6738 7006 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1229 987 891 787 597 393 268
213 212 127 63
EcoNI CCTnn'n_nnAGG
Cuts at: 3488 3488
Size: 7291
EcoO109I rG'GnC_Cy
Cuts at: 2106 2133 2895 3745 4226 4524 2106
Size: 27 762 850 481 298 4873
Fragments arranged by size:
4873 850 762 481 298 27
EcoRI G'AATT_C
Cuts at: 4600 4978 4600
Size: 378 6913
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2056 2056
Size: 7291
FspI TGC'GCA
Cuts at: 5797 5797
Size: 7291
HaeII r_GCGC'y
Cuts at: 1678 2531 3604 6438 1678
Size: 853 1073 2834 2531
Fragments arranged by size:
2834 2531 1073 853
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3237 3269 4205 4237 4550
Size: 32 135 32 1665 32 936 32 313
Cuts at: 4550 4582 5932 5964 6006 6038 1373
Size: 32 1350 32 42 32 2626
Fragments arranged by size:
2626 1665 1350 936 313 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2746 3796 4635 4881 669
Size: 988 281 808 1050 839 246 3079
Fragments arranged by size:
3079 1050 988 839 808 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7291
HpaI GTT'AAC
Cuts at: 2746 4881 2746
Size: 2135 5156
KasI G'GCGC_C
Cuts at: 3600 3600
Size: 7291
KpnI G_GTAC'C
Cuts at: 4616 4616
Size: 7291
MfeI C'AATT_G
Cuts at: 2052 4890 2052
Size: 2838 4453
MluI A'CGCG_T
Cuts at: 3216 7167 3216
Size: 3951 3340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2687 6405 6589 1305
Size: 1382 3718 184 2007
Fragments arranged by size:
3718 2007 1382 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2145 2933 10
Size: 54 1425 343 313 788 4368
Fragments arranged by size:
4368 1425 788 343 313 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3179 3509 3548 3942 4476 5249 5608
Size: 648 2013 330 39 394 534 773 359
Cuts at: 5608 5767 518
Size: 159 2042
Fragments arranged by size:
2042 2013 773 648 534 394 359 330
159 39
MspA1I CmG'CkG
Cuts at: 1606 3139 3418 3706 3999 4063 4476 4707 5384
Size: 1533 279 288 293 64 413 231 677
Cuts at: 5384 6532 6777 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1533 1148 677 413 293 288 279
245 231 64
NaeI GCC'GGC
Cuts at: 3157 3605 3157
Size: 448 6843
NarI GG'CG_CC
Cuts at: 3601 3601
Size: 7291
NcoI C'CATG_G
Cuts at: 513 1065 2425 2493 513
Size: 552 1360 68 5311
Fragments arranged by size:
5311 1360 552 68
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7291
NgoMIV G'CCGG_C
Cuts at: 3155 3603 3155
Size: 448 6843
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7291
NotI GC'GGCC_GC
Cuts at: 4738 4738
Size: 7291
NspI r_CATG'y
Cuts at: 1132 1229 4165 4451 4649 6194 1132
Size: 97 2936 286 198 1545 2229
Fragments arranged by size:
2936 2229 1545 286 198 97
PasI CC'CwG_GG
Cuts at: 1348 2668 3721 4529 1348
Size: 1320 1053 808 4110
Fragments arranged by size:
4110 1320 1053 808
PciI A'CATG_T
Cuts at: 6190 6190
Size: 7291
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2554 3748 3760 4183 4432 1108
Size: 1446 1194 12 423 249 3967
Fragments arranged by size:
3967 1446 1194 423 249 12
PfoI T'CCnGG_A
Cuts at: 3332 4425 3332
Size: 1093 6198
PmeI GTTT'AAAC
Cuts at: 4595 4595
Size: 7291
PmlI CAC'GTG
Cuts at: 2185 2185
Size: 7291
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5360 5392 6873 6905 5360
Size: 32 1481 32 5746
Fragments arranged by size:
5746 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2133 3745 2133
Size: 1612 5679
PshAI GACnn'nnGTC
Cuts at: 1613 2171 1613
Size: 558 6733
PsiI TTA'TAA
Cuts at: 4861 4861
Size: 7291
PspOMI G'GGCC_C
Cuts at: 3311 4225 3311
Size: 914 6377
PstI C_TGCA'G
Cuts at: 830 2313 2736 3288 4319 4643 830
Size: 1483 423 552 1031 324 3478
Fragments arranged by size:
3478 1483 1031 552 423 324
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7291
PvuII CAG'CTG
Cuts at: 3706 3706
Size: 7291
SacI G_AGCT'C
Cuts at: 3321 3501 3996 3321
Size: 180 495 6616
Fragments arranged by size:
6616 495 180
SacII CC_GC'GG
Cuts at: 3140 4477 3140
Size: 1337 5954
SalI G'TCGA_C
Cuts at: 1936 3794 4633 1936
Size: 1858 839 4594
Fragments arranged by size:
4594 1858 839
SapI GCTCTTCn'nnn_
Cuts at: 2944 3326 2944
Size: 382 6909
SbfI CC_TGCA'GG
Cuts at: 2313 4643 2313
Size: 2330 4961
ScaI AGT'ACT
Cuts at: 5539 7251 5539
Size: 1712 5579
SexAI A'CCwGG_T
Cuts at: 2582 2582
Size: 7291
SfcI C'TryA_G
Cuts at: 826 1045 2309 2732 3284 4315 4639 5774 6455
Size: 219 1264 423 552 1031 324 1135 681
Cuts at: 6455 6646 826
Size: 191 1471
Fragments arranged by size:
1471 1264 1135 1031 681 552 423 324
219 191
SfoI GGC'GCC
Cuts at: 3602 3602
Size: 7291
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7291
SgrAI Cr'CCGG_yG
Cuts at: 1700 3155 1700
Size: 1455 5836
SmaI CCC'GGG
Cuts at: 1872 4377 4618 1872
Size: 2505 241 4545
Fragments arranged by size:
4545 2505 241
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3608 5395 6296 6558 820
Size: 197 926 1665 1787 901 262 1553
Fragments arranged by size:
1787 1665 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5082 493
Size: 4589 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7291
SphI G_CATG'C
Cuts at: 1132 4165 4451 4649 1132
Size: 3033 286 198 3774
Fragments arranged by size:
3774 3033 286 198
SspI AAT'ATT
Cuts at: 5 52 2600 5215 5
Size: 47 2548 2615 2081
Fragments arranged by size:
2615 2548 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7291
StyI C'CwwG_G
Cuts at: 513 1065 2040 2425 2493 2503 2739 4554 4730
Size: 552 975 385 68 10 236 1815 176
Cuts at: 4730 513
Size: 3074
Fragments arranged by size:
3074 1815 975 552 385 236 176 68
10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2716 2799 3044 4101 5318 5335 5488
Size: 1255 1136 83 245 1057 1217 17 153
Cuts at: 5488 5673 6118 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1217 1136 1057 445 245 185
153 83 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 3234 3900
Size: 274 80 33 51 1443 147 1110 666
Cuts at: 3900 5537 7249 96
Size: 1637 1712 138
Fragments arranged by size:
1712 1637 1443 1110 666 274 147 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2602 2634 2664 2696 7251 7283 2602
Size: 32 30 32 4555 32 2610
Fragments arranged by size:
4555 2610 32 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 1316 4418 1316
Size: 3102 4189
XbaI T'CTAG_A
Cuts at: 2069 4627 2069
Size: 2558 4733
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2126 2293 2721 2941 3808 4237 2126
Size: 167 428 220 867 429 5180
Fragments arranged by size:
5180 867 429 428 220 167
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7291
XmaI C'CCGG_G
Cuts at: 1870 4375 4616 1870
Size: 2505 241 4545
Fragments arranged by size:
4545 2505 241
XmnI GAAnn'nnTTC
Cuts at: 5420 5420
Size: 7291
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2169 5099 276
Size: 53 83 186 936 635 2930 2468
Fragments arranged by size:
2930 2468 936 635 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI Bpu10I BsiWI
BstXI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI
PacI PspXI PsrI RsrII SanDI SfiI SrfI SwaI