Restriction map for
pFN21AB8646
(Circular) (Six-base) MAPSORT of: pFN21AB8646.seq Check: 4234 from: 1 to: 5422
pFN21AB8646 5422 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2024 3232 278
Size: 53 83 186 936 488 1208 2468
Fragments arranged by size:
2468 1208 936 488 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2765 1937
Size: 828 4594
Acc65I G'GTAC_C
Cuts at: 2743 2743
Size: 5422
AclI AA'CG_TT
Cuts at: 1563 3549 3922 1563
Size: 1986 373 3063
Fragments arranged by size:
3063 1986 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3485 4869 5137 1632
Size: 1853 1384 268 1917
Fragments arranged by size:
1917 1853 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5225
AflIII A'CryG_T
Cuts at: 1203 4321 5298 1203
Size: 3118 977 1327
Fragments arranged by size:
3118 1327 977
AgeI A'CCGG_T
Cuts at: 4253 4253
Size: 5422
AhdI GACnn_n'nnGTC
Cuts at: 4151 4151
Size: 5422
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5422
AlwNI CAG_nnn'CTG
Cuts at: 3165 4737 3165
Size: 1572 3850
ApaLI G'TGCA_C
Cuts at: 3479 4635 3479
Size: 1156 4266
ApoI r'AATT_y
Cuts at: 1493 2731 2957 3109 5218 1493
Size: 1238 226 152 2109 1697
Fragments arranged by size:
2109 1697 1238 226 152
AscI GG'CGCG_CC
Cuts at: 2560 2560
Size: 5422
AseI AT'TA_AT
Cuts at: 160 1032 3976 160
Size: 872 2944 1606
Fragments arranged by size:
2944 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
AvaI C'yCGr_G
Cuts at: 1870 1943 2224 2309 2395 2706 2747 1870
Size: 73 281 85 86 311 41 4545
Fragments arranged by size:
4545 311 281 86 85 73 41
BamHI G'GATC_C
Cuts at: 2220 2752 3119 2220
Size: 532 367 4523
Fragments arranged by size:
4523 532 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2743 4198 618
Size: 325 217 627 956 1455 1842
Fragments arranged by size:
1842 1455 956 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2233 2316 2741 1391
Size: 842 83 425 4072
Fragments arranged by size:
4072 842 425 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2582 928
Size: 971 683 3768
Fragments arranged by size:
3768 971 683
BbvCI CC'TCA_GC
Cuts at: 2120 2120
Size: 5422
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3613 3647 1903
Size: 34 1676 34 3678
Fragments arranged by size:
3678 1676 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3314 4524 3314
Size: 1210 4212
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4033 136
Size: 107 122 71 631 605 2361 1525
Fragments arranged by size:
2361 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5417 5417
Size: 5422
BlpI GC'TnA_GC
Cuts at: 2839 2839
Size: 5422
Bme1580I G_kGCm'C
Cuts at: 1790 2645 3483 4639 1790
Size: 855 838 1156 2573
Fragments arranged by size:
2573 1156 855 838
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5422
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2448 2651 3153
Size: 416 53 83 399 24 1031 203 502
Cuts at: 3153 4111 442
Size: 958 1753
Fragments arranged by size:
1753 1031 958 502 416 399 203 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 4082 1239
Size: 956 1887 2579
Fragments arranged by size:
2579 1887 956
Bpu10I CC'TnA_GC
Cuts at: 2120 2120
Size: 5422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3547 4412 4710 3547
Size: 865 298 4259
Fragments arranged by size:
4259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4085 882
Size: 617 2586 2219
Fragments arranged by size:
2586 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3213 493
Size: 711 2009 2702
Fragments arranged by size:
2702 2009 711
BsaBI GATnn'nnATC
Cuts at: 5416 5416
Size: 5422
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 3229 3611 4246
Size: 53 83 186 936 488 1208 382 635
Cuts at: 4246 275
Size: 1451
Fragments arranged by size:
1451 1208 936 635 488 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3855 4253 4527 4674 5085 3855
Size: 398 274 147 411 4192
Fragments arranged by size:
4192 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2278 2308 1178
Size: 30 165 30 875 30 4292
Fragments arranged by size:
4292 875 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2245 1186
Size: 1059 4363
BseYI C'CCAG_C
Cuts at: 2038 4625 5121 2038
Size: 2587 496 2339
Fragments arranged by size:
2587 2339 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2030 1163
Size: 573 294 4555
Fragments arranged by size:
4555 573 294
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2872 3633 4661 1540
Size: 275 186 871 761 1028 2301
Fragments arranged by size:
2301 1028 871 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2713 3483 3568 4639 2713
Size: 770 85 1071 3496
Fragments arranged by size:
3496 1071 770 85
BsmI GAATG_Cn'
Cuts at: 2065 2933 3026 2065
Size: 868 93 4461
Fragments arranged by size:
4461 868 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2058 2058
Size: 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2233 2316 2645 2713 2741 3483 3568
Size: 399 443 83 329 68 28 742 85
Cuts at: 3568 4639 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 443 399 329 85 83
68 28
BspEI T'CCGG_A
Cuts at: 5085 5085
Size: 5422
BspHI T'CATG_A
Cuts at: 2370 3311 5041 2370
Size: 941 1730 2751
Fragments arranged by size:
2751 1730 941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2777 844
Size: 1933 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2545 3309 1394
Size: 429 722 764 3507
Fragments arranged by size:
3507 764 722 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3917 4091 65
Size: 1183 2669 174 1396
Fragments arranged by size:
2669 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2334 4066 4253 1700
Size: 110 524 1732 187 2869
Fragments arranged by size:
2869 1732 524 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5422
BssHII G'CGCG_C
Cuts at: 1925 2560 1925
Size: 635 4787
BssSI C'ACGA_G
Cuts at: 3482 4494 3482
Size: 1012 4410
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5422
BstBI TT'CG_AA
Cuts at: 4226 4226
Size: 5422
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5422
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2173
Size: 5422
BstYI r'GATC_y
Cuts at: 1972 2220 2752 3119 3504 3521 4973 5059 5071
Size: 248 532 367 385 17 1452 86 12
Cuts at: 5071 5417 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 532 385 367 346 248 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3931
Fragments arranged by size:
3931 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4683
Fragments arranged by size:
4683 683 56
BtsI GCAGTG_nn'
Cuts at: 2938 3732 3752 2938
Size: 794 20 4608
Fragments arranged by size:
4608 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5422
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5387
DraI TTT'AAA
Cuts at: 2726 2726
Size: 5422
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4429 809
Size: 507 3113 1802
Fragments arranged by size:
3113 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2869 3758 5387
Size: 54 1425 50 275 18 1039 889 1629
Cuts at: 5387 8
Size: 43
Fragments arranged by size:
1629 1425 1039 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2869 1537
Size: 275 1057 4090
Fragments arranged by size:
4090 1057 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3352 1353
Size: 1999 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2162 2462 4009 4383 4529 387
Size: 1064 138 573 300 1547 374 146 1280
Fragments arranged by size:
1547 1280 1064 573 374 300 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 3485 4082 4869 5137 1239
Size: 393 563 1290 597 787 268 1524
Fragments arranged by size:
1524 1290 787 597 563 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2590 2590
Size: 5422
EcoO109I rG'GnC_Cy
Cuts at: 2326 2648 2326
Size: 322 5100
EcoRI G'AATT_C
Cuts at: 2731 3109 2731
Size: 378 5044
FspI TGC'GCA
Cuts at: 2492 3928 2492
Size: 1436 3986
HaeII r_GCGC'y
Cuts at: 1678 2120 2693 4569 1678
Size: 442 573 1876 2531
Fragments arranged by size:
2531 1876 573 442
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2278 2310 2358 2390 4063
Size: 32 135 32 706 32 48 32 1673
Cuts at: 4063 4095 4137 4169 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1673 706 135 48 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2766 3012 669
Size: 988 281 828 246 3079
Fragments arranged by size:
3079 988 828 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5422
HpaI GTT'AAC
Cuts at: 3012 3012
Size: 5422
KpnI G_GTAC'C
Cuts at: 2747 2747
Size: 5422
MfeI C'AATT_G
Cuts at: 3021 3021
Size: 5422
MluI A'CGCG_T
Cuts at: 5298 5298
Size: 5422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2321 2697 4536 4720 1305
Size: 1016 376 1839 184 2007
Fragments arranged by size:
2007 1839 1016 376 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3600
Fragments arranged by size:
3600 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 3380 3739 3898 518
Size: 648 1002 1212 359 159 2042
Fragments arranged by size:
2042 1212 1002 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2629 2838 3515 4663 4908 1606
Size: 1023 209 677 1148 245 2120
Fragments arranged by size:
2120 1148 1023 677 245 209
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3931
Fragments arranged by size:
3931 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5422
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5422
NotI GC'GGCC_GC
Cuts at: 2869 2869
Size: 5422
NspI r_CATG'y
Cuts at: 1132 1229 2173 2473 2555 2780 4325 1132
Size: 97 944 300 82 225 1545 2229
Fragments arranged by size:
2229 1545 944 300 225 97 82
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5422
PciI A'CATG_T
Cuts at: 4321 4321
Size: 5422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5422
PmeI GTTT'AAAC
Cuts at: 2726 2726
Size: 5422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 5004 5036 3491
Size: 32 1481 32 3877
Fragments arranged by size:
3877 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2326 2326
Size: 5422
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5422
PsiI TTA'TAA
Cuts at: 2992 2992
Size: 5422
PstI C_TGCA'G
Cuts at: 830 2238 2774 830
Size: 1408 536 3478
Fragments arranged by size:
3478 1408 536
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5422
PvuII CAG'CTG
Cuts at: 2629 2629
Size: 5422
SalI G'TCGA_C
Cuts at: 1936 2764 1936
Size: 828 4594
SanDI GG'GwC_CC
Cuts at: 2326 2326
Size: 5422
SbfI CC_TGCA'GG
Cuts at: 2774 2774
Size: 5422
ScaI AGT'ACT
Cuts at: 3670 5382 3670
Size: 1712 3710
SfcI C'TryA_G
Cuts at: 826 1045 2234 2770 3905 4586 4777 826
Size: 219 1189 536 1135 681 191 1471
Fragments arranged by size:
1471 1189 1135 681 536 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5422
SmaI CCC'GGG
Cuts at: 1872 2226 2397 2749 1872
Size: 354 171 352 4545
Fragments arranged by size:
4545 354 352 171
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3526 4427 4689 820
Size: 197 926 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3213 493
Size: 2720 2702
SpeI A'CTAG_T
Cuts at: 152 2681 152
Size: 2529 2893
SphI G_CATG'C
Cuts at: 1132 2555 2780 1132
Size: 1423 225 3774
Fragments arranged by size:
3774 1423 225
SspI AAT'ATT
Cuts at: 5 52 3346 5
Size: 47 3294 2081
Fragments arranged by size:
3294 2081 47
StuI AGG'CCT
Cuts at: 1517 2384 1517
Size: 867 4555
StyI C'CwwG_G
Cuts at: 513 1065 2004 2043 2861 513
Size: 552 939 39 818 3074
Fragments arranged by size:
3074 939 818 552 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3449 3466 3619 3804 4249 325
Size: 1255 1869 17 153 185 445 1498
Fragments arranged by size:
1869 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3668 5380 96
Size: 274 80 33 51 1443 1691 1712 138
Fragments arranged by size:
1712 1691 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5382 5414 5382
Size: 32 5390
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5422
XbaI T'CTAG_A
Cuts at: 2758 2758
Size: 5422
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5422
XmaI C'CCGG_G
Cuts at: 1870 2224 2395 2747 1870
Size: 354 171 352 4545
Fragments arranged by size:
4545 354 352 171
XmnI GAAnn'nnTTC
Cuts at: 3551 3551
Size: 5422
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2022 3230 276
Size: 53 83 186 936 488 1208 2468
Fragments arranged by size:
2468 1208 936 488 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI