Restriction map for
pFN21AB8642
(Circular) (Six-base) MAPSORT of: pFN21AB8642.seq Check: 5918 from: 1 to: 7978
pFN21AB8642 7978 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5788 278
Size: 53 83 186 936 4252 2468
Fragments arranged by size:
4252 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5061 5321 1937
Size: 3124 260 4594
Fragments arranged by size:
4594 3124 260
Acc65I G'GTAC_C
Cuts at: 5299 5299
Size: 7978
AclI AA'CG_TT
Cuts at: 1563 2773 4978 6105 6478 1563
Size: 1210 2205 1127 373 3063
Fragments arranged by size:
3063 2205 1210 1127 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3623 4043 4443 5126 6041 7425 7693 1632
Size: 1991 420 400 683 915 1384 268 1917
Fragments arranged by size:
1991 1917 1384 915 683 420 400 268
AflII C'TTAA_G
Cuts at: 820 1017 4394 820
Size: 197 3377 4404
Fragments arranged by size:
4404 3377 197
AflIII A'CryG_T
Cuts at: 1203 6877 7854 1203
Size: 5674 977 1327
Fragments arranged by size:
5674 1327 977
AgeI A'CCGG_T
Cuts at: 6809 6809
Size: 7978
AhdI GACnn_n'nnGTC
Cuts at: 6707 6707
Size: 7978
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7978
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7978
AlwNI CAG_nnn'CTG
Cuts at: 2564 5721 7293 2564
Size: 3157 1572 3249
Fragments arranged by size:
3249 3157 1572
ApaLI G'TGCA_C
Cuts at: 6035 7191 6035
Size: 1156 6822
ApoI r'AATT_y
Cuts at: 1493 2026 2627 3277 4173 4355 4879 5051 5193
Size: 533 601 650 896 182 524 172 142
Cuts at: 5193 5287 5513 5665 7774 1493
Size: 94 226 152 2109 1697
Fragments arranged by size:
2109 1697 896 650 601 533 524 226
182 172 152 142 94
AseI AT'TA_AT
Cuts at: 160 1032 4365 4617 6532 160
Size: 872 3333 252 1915 1606
Fragments arranged by size:
3333 1915 1606 872 252
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7978
AvaI C'yCGr_G
Cuts at: 1870 1943 5303 1870
Size: 73 3360 4545
Fragments arranged by size:
4545 3360 73
BamHI G'GATC_C
Cuts at: 2312 5308 5675 2312
Size: 2996 367 4615
Fragments arranged by size:
4615 2996 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4119 5299 6754 618
Size: 325 217 627 2332 1180 1455 1842
Fragments arranged by size:
2332 1842 1455 1180 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5297 1391
Size: 3906 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2106 2813 928
Size: 971 207 707 6093
Fragments arranged by size:
6093 971 707 207
BbvCI CC'TCA_GC
Cuts at: 3093 3093
Size: 7978
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6169 6203 1903
Size: 34 4232 34 3678
Fragments arranged by size:
4232 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4477 5870 7080 4477
Size: 1393 1210 5375
Fragments arranged by size:
5375 1393 1210
BclI T'GATC_A
Cuts at: 1548 2530 3928 1548
Size: 982 1398 5598
Fragments arranged by size:
5598 1398 982
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6589 136
Size: 107 122 71 631 605 4917 1525
Fragments arranged by size:
4917 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7973 7973
Size: 7978
BlpI GC'TnA_GC
Cuts at: 5395 5395
Size: 7978
Bme1580I G_kGCm'C
Cuts at: 1790 6039 7195 1790
Size: 4249 1156 2573
Fragments arranged by size:
4249 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7978
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4769 5709 6667
Size: 416 53 83 399 24 3352 940 958
Cuts at: 6667 442
Size: 1753
Fragments arranged by size:
3352 1753 958 940 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4385 1056
Size: 3329 4649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4944 4989 6638 1239
Size: 3705 45 1649 2579
Fragments arranged by size:
3705 2579 1649 45
Bpu10I CC'TnA_GC
Cuts at: 3093 3983 4239 3093
Size: 890 256 6832
Fragments arranged by size:
6832 890 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4205 5238 6103 6968 7266 4205
Size: 1033 865 865 298 4917
Fragments arranged by size:
4917 1033 865 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4697 6641 882
Size: 617 3198 1944 2219
Fragments arranged by size:
3198 2219 1944 617
BsaAI yAC'GTr
Cuts at: 493 1204 5769 493
Size: 711 4565 2702
Fragments arranged by size:
4565 2702 711
BsaBI GATnn'nnATC
Cuts at: 3927 7972 3927
Size: 4045 3933
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5785 6167 6802 275
Size: 53 83 186 936 4252 382 635 1451
Fragments arranged by size:
4252 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6411 6809 7083 7230 7641 6411
Size: 398 274 147 411 6748
Fragments arranged by size:
6748 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4529 4559 1178
Size: 30 165 30 3126 30 4597
Fragments arranged by size:
4597 3126 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2397 4550 1186
Size: 1211 2153 4614
Fragments arranged by size:
4614 2153 1211
BseYI C'CCAG_C
Cuts at: 5121 7181 7677 5121
Size: 2060 496 5422
Fragments arranged by size:
5422 2060 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2274 3888 1163
Size: 573 538 1614 5253
Fragments arranged by size:
5253 1614 573 538
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5428 6189 7217 1540
Size: 275 186 3427 761 1028 2301
Fragments arranged by size:
3427 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3034 4779 6039 6124 7195 3034
Size: 1745 1260 85 1071 3817
Fragments arranged by size:
3817 1745 1260 1071 85
BsmI GAATG_Cn'
Cuts at: 2881 3537 5489 5582 2881
Size: 656 1952 93 5277
Fragments arranged by size:
5277 1952 656 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3034 4779 5297 6039 6124 7195 1391
Size: 399 1244 1745 518 742 85 1071 2174
Fragments arranged by size:
2174 1745 1244 1071 742 518 399 85
BspEI T'CCGG_A
Cuts at: 7641 7641
Size: 7978
BspHI T'CATG_A
Cuts at: 2634 3648 5867 7597 2634
Size: 1014 2219 1730 3015
Fragments arranged by size:
3015 2219 1730 1014
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2938 5333 844
Size: 2094 2395 3489
Fragments arranged by size:
3489 2395 2094
BsrBI CCG'CTC
Cuts at: 1394 1823 2731 5865 1394
Size: 429 908 3134 3507
Fragments arranged by size:
3507 3134 908 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5231 6473 6647 65
Size: 1183 3983 1242 174 1396
Fragments arranged by size:
3983 1396 1242 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6622 6809 1700
Size: 110 4812 187 2869
Fragments arranged by size:
4812 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7978
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7978
BssSI C'ACGA_G
Cuts at: 2500 3027 3864 6038 7050 2500
Size: 527 837 2174 1012 3428
Fragments arranged by size:
3428 2174 1012 837 527
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4649 794
Size: 3855 4123
BstBI TT'CG_AA
Cuts at: 6782 6782
Size: 7978
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7978
BstYI r'GATC_y
Cuts at: 1972 2312 5308 5675 6060 6077 7529 7615 7627
Size: 340 2996 367 385 17 1452 86 12
Cuts at: 7627 7973 1972
Size: 346 1977
Fragments arranged by size:
2996 1977 1452 385 367 346 340 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2490 5091 513
Size: 552 939 486 2601 3400
Fragments arranged by size:
3400 2601 939 552 486
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5005 487
Size: 683 56 3779 3460
Fragments arranged by size:
3779 3460 683 56
BtsI GCAGTG_nn'
Cuts at: 2853 5494 6288 6308 2853
Size: 2641 794 20 4523
Fragments arranged by size:
4523 2641 794 20
ClaI AT'CG_AT
Cuts at: 1554 4621 1554
Size: 3067 4911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2224 2259 529
Size: 35 1660 35 6248
Fragments arranged by size:
6248 1660 35 35
DraI TTT'AAA
Cuts at: 2322 2398 4740 5282 2322
Size: 76 2342 542 5018
Fragments arranged by size:
5018 2342 542 76
DraIII CAC_nnn'GTG
Cuts at: 4995 4995
Size: 7978
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6985 809
Size: 507 5669 1802
Fragments arranged by size:
5669 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3680 5425
Size: 54 1425 50 275 18 177 1673 1745
Cuts at: 5425 6314 7943 8
Size: 889 1629 43
Fragments arranged by size:
1745 1673 1629 1425 889 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5425 1537
Size: 275 3613 4090
Fragments arranged by size:
4090 3613 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2243 2284 2505 2721 3009 3165 3468 5908
Size: 890 41 221 216 288 156 303 2440
Cuts at: 5908 1353
Size: 3423
Fragments arranged by size:
3423 2440 890 303 288 221 216 156
41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6565 6939 7085 387
Size: 1064 138 4976 374 146 1280
Fragments arranged by size:
4976 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3623 4043 4443 4944 4989 5126 6041
Size: 393 1991 420 400 501 45 137 915
Cuts at: 6041 6638 7425 7693 1239
Size: 597 787 268 1524
Fragments arranged by size:
1991 1524 915 787 597 501 420 400
393 268 137 45
EcoNI CCTnn'n_nnAGG
Cuts at: 4926 5217 4926
Size: 291 7687
EcoO109I rG'GnC_Cy
Cuts at: 3078 3521 3550 3634 3672 3078
Size: 443 29 84 38 7384
Fragments arranged by size:
7384 443 84 38 29
EcoRI G'AATT_C
Cuts at: 5193 5287 5665 5193
Size: 94 378 7506
Fragments arranged by size:
7506 378 94
EcoRV GAT'ATC
Cuts at: 2359 2359
Size: 7978
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2478 2611 2750 2478
Size: 133 139 7706
Fragments arranged by size:
7706 139 133
FspI TGC'GCA
Cuts at: 6484 6484
Size: 7978
HaeII r_GCGC'y
Cuts at: 1678 2752 7125 1678
Size: 1074 4373 2531
Fragments arranged by size:
4373 2531 1074
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2877 2909 3373 3405 4039
Size: 32 135 32 1305 32 464 32 634
Cuts at: 4039 4071 4529 4561 5124 5156 6619 6651 6693
Size: 32 458 32 563 32 1463 32 42
Cuts at: 6693 6725 1373
Size: 32 2626
Fragments arranged by size:
2626 1463 1305 634 563 464 458 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2218 5322 5568 669
Size: 988 281 280 3104 246 3079
Fragments arranged by size:
3104 3079 988 281 280 246
HindIII A'AGCT_T
Cuts at: 748 3909 748
Size: 3161 4817
HpaI GTT'AAC
Cuts at: 5568 5568
Size: 7978
KpnI G_GTAC'C
Cuts at: 5303 5303
Size: 7978
MfeI C'AATT_G
Cuts at: 2870 5577 2870
Size: 2707 5271
MluI A'CGCG_T
Cuts at: 7854 7854
Size: 7978
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2119 3195 3782 7092 7276 1305
Size: 814 1076 587 3310 184 2007
Fragments arranged by size:
3310 2007 1076 814 587 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 5979
Fragments arranged by size:
5979 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2546 3037 3618 3624 4893 5069 5936
Size: 648 1380 491 581 6 1269 176 867
Cuts at: 5936 6295 6454 518
Size: 359 159 2042
Fragments arranged by size:
2042 1380 1269 867 648 581 491 359
176 159 6
MspA1I CmG'CkG
Cuts at: 1606 4858 5394 6071 7219 7464 1606
Size: 3252 536 677 1148 245 2120
Fragments arranged by size:
3252 2120 1148 677 536 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 5091 513
Size: 552 939 3087 3400
Fragments arranged by size:
3400 3087 939 552
NdeI CA'TA_TG
Cuts at: 387 4278 387
Size: 3891 4087
NheI G'CTAG_C
Cuts at: 1052 4381 1052
Size: 3329 4649
NotI GC'GGCC_GC
Cuts at: 5425 5425
Size: 7978
NspI r_CATG'y
Cuts at: 1132 1229 3377 4652 5336 6881 1132
Size: 97 2148 1275 684 1545 2229
Fragments arranged by size:
2229 2148 1545 1275 684 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7978
PciI A'CATG_T
Cuts at: 6877 6877
Size: 7978
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3199 1108
Size: 2091 5887
PmeI GTTT'AAAC
Cuts at: 5282 5282
Size: 7978
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6047 6079 7560 7592 6047
Size: 32 1481 32 6433
Fragments arranged by size:
6433 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3550 3634 3672 3550
Size: 84 38 7856
Fragments arranged by size:
7856 84 38
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7978
PsiI TTA'TAA
Cuts at: 2466 4134 5548 2466
Size: 1668 1414 4896
Fragments arranged by size:
4896 1668 1414
PstI C_TGCA'G
Cuts at: 830 2131 2356 3600 5330 830
Size: 1301 225 1244 1730 3478
Fragments arranged by size:
3478 1730 1301 1244 225
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7978
SalI G'TCGA_C
Cuts at: 1936 5320 1936
Size: 3384 4594
SanDI GG'GwC_CC
Cuts at: 3550 3550
Size: 7978
SapI GCTCTTCn'nnn_
Cuts at: 2284 2505 2721 3165 3468 2284
Size: 221 216 444 303 6794
Fragments arranged by size:
6794 444 303 221 216
SbfI CC_TGCA'GG
Cuts at: 5330 5330
Size: 7978
ScaI AGT'ACT
Cuts at: 2259 3187 6226 7938 2259
Size: 928 3039 1712 2299
Fragments arranged by size:
3039 2299 1712 928
SfcI C'TryA_G
Cuts at: 826 1045 2127 2352 3516 3596 3858 4209 5326
Size: 219 1082 225 1164 80 262 351 1117
Cuts at: 5326 6461 7142 7333 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1164 1135 1117 1082 681 351 262
225 219 191 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7978
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7978
SmaI CCC'GGG
Cuts at: 1872 5305 1872
Size: 3433 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4184 4394 5253 6082 6983 7245
Size: 197 926 2241 210 859 829 901 262
Cuts at: 7245 820
Size: 1553
Fragments arranged by size:
2241 1553 926 901 859 829 262 210
197
SnaBI TAC'GTA
Cuts at: 493 5769 493
Size: 5276 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7978
SphI G_CATG'C
Cuts at: 1132 3377 5336 1132
Size: 2245 1959 3774
Fragments arranged by size:
3774 2245 1959
SspI AAT'ATT
Cuts at: 5 52 4165 5902 5
Size: 47 4113 1737 2081
Fragments arranged by size:
4113 2081 1737 47
StuI AGG'CCT
Cuts at: 1517 3260 3602 1517
Size: 1743 342 5893
Fragments arranged by size:
5893 1743 342
StyI C'CwwG_G
Cuts at: 513 1065 2004 5091 5417 513
Size: 552 939 3087 326 3074
Fragments arranged by size:
3087 3074 939 552 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2267 3907 6005 6022 6175 6360 6805
Size: 1255 687 1640 2098 17 153 185 445
Cuts at: 6805 325
Size: 1498
Fragments arranged by size:
2098 1640 1498 1255 687 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2257 2798 2976
Size: 274 80 33 51 1443 280 541 178
Cuts at: 2976 3185 6224 7936 96
Size: 209 3039 1712 138
Fragments arranged by size:
3039 1712 1443 541 280 274 209 178
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7938 7970 7938
Size: 32 7946
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7978
XbaI T'CTAG_A
Cuts at: 5314 5314
Size: 7978
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7978
XmaI C'CCGG_G
Cuts at: 1870 5303 1870
Size: 3433 4545
XmnI GAAnn'nnTTC
Cuts at: 2775 3303 6107 2775
Size: 528 2804 4646
Fragments arranged by size:
4646 2804 528
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5786 276
Size: 53 83 186 936 4252 2468
Fragments arranged by size:
4252 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI