Restriction map for
pFN21AB8638
(Circular) (Six-base) MAPSORT of: pFN21AB8638.seq Check: 484 from: 1 to: 5827
pFN21AB8638 5827 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3637 278
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3170 1937
Size: 1233 4594
Acc65I G'GTAC_C
Cuts at: 3148 3148
Size: 5827
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2769 3060 3137 3890 5274 5542 1632
Size: 1137 291 77 753 1384 268 1917
Fragments arranged by size:
1917 1384 1137 753 291 268 77
AfeI AGC'GCT
Cuts at: 2855 2855
Size: 5827
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5630
AflIII A'CryG_T
Cuts at: 1203 2106 4726 5703 1203
Size: 903 2620 977 1327
Fragments arranged by size:
2620 1327 977 903
AgeI A'CCGG_T
Cuts at: 4658 4658
Size: 5827
AhdI GACnn_n'nnGTC
Cuts at: 4556 4556
Size: 5827
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5827
AlwNI CAG_nnn'CTG
Cuts at: 2834 3570 5142 2834
Size: 736 1572 3519
Fragments arranged by size:
3519 1572 736
ApaLI G'TGCA_C
Cuts at: 3884 5040 3884
Size: 1156 4671
ApoI r'AATT_y
Cuts at: 1493 2096 2306 2312 2408 2590 2633 2916 3136
Size: 603 210 6 96 182 43 283 220
Cuts at: 3136 3362 3514 5623 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 603 283 226 220 210 182
152 96 43 6
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 3152 1870
Size: 73 1209 4545
Fragments arranged by size:
4545 1209 73
BamHI G'GATC_C
Cuts at: 3157 3524 3157
Size: 367 5460
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3148 4603 618
Size: 325 217 627 1361 1455 1842
Fragments arranged by size:
1842 1455 1361 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3146 1391
Size: 1755 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4856
BbvCI CC'TCA_GC
Cuts at: 3054 3054
Size: 5827
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2025 2059 4018 4052 1903
Size: 34 88 34 1959 34 3678
Fragments arranged by size:
3678 1959 88 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3719 4929 3719
Size: 1210 4617
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4438 136
Size: 107 122 71 631 605 2766 1525
Fragments arranged by size:
2766 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5822 5822
Size: 5827
BlpI GC'TnA_GC
Cuts at: 3244 3244
Size: 5827
Bme1580I G_kGCm'C
Cuts at: 1790 3089 3888 5044 1790
Size: 1299 799 1156 2573
Fragments arranged by size:
2573 1299 1156 799
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5827
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2404 3558 4516
Size: 416 53 83 399 24 987 1154 958
Cuts at: 4516 442
Size: 1753
Fragments arranged by size:
1753 1154 987 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2870 4487 1239
Size: 1631 1617 2579
Fragments arranged by size:
2579 1631 1617
Bpu10I CC'TnA_GC
Cuts at: 3054 3054
Size: 5827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3952 4817 5115 3952
Size: 865 298 4664
Fragments arranged by size:
4664 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2999 4490 882
Size: 617 1500 1491 2219
Fragments arranged by size:
2219 1500 1491 617
BsaAI yAC'GTr
Cuts at: 493 1204 3618 493
Size: 711 2414 2702
Fragments arranged by size:
2702 2414 711
BsaBI GATnn'nnATC
Cuts at: 5821 5821
Size: 5827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3634 4016 4651 275
Size: 53 83 186 936 2101 382 635 1451
Fragments arranged by size:
2101 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2671 4260 4658 4932 5079 5490 2671
Size: 1589 398 274 147 411 3008
Fragments arranged by size:
3008 1589 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5602
Fragments arranged by size:
5602 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2720 1186
Size: 1534 4293
BseYI C'CCAG_C
Cuts at: 5030 5526 5030
Size: 496 5331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2964 1163
Size: 573 1228 4026
Fragments arranged by size:
4026 1228 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3277 4038 5066 1540
Size: 275 186 1276 761 1028 2301
Fragments arranged by size:
2301 1276 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3888 3973 5044 3888
Size: 85 1071 4671
Fragments arranged by size:
4671 1071 85
BsmI GAATG_Cn'
Cuts at: 2332 3338 3431 2332
Size: 1006 93 4728
Fragments arranged by size:
4728 1006 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3089 3146 3888 3973 5044 1391
Size: 399 1299 57 742 85 1071 2174
Fragments arranged by size:
2174 1299 1071 742 399 85 57
BspEI T'CCGG_A
Cuts at: 5490 5490
Size: 5827
BspHI T'CATG_A
Cuts at: 3716 5446 3716
Size: 1730 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3182 844
Size: 2338 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3066 3714 1394
Size: 429 1243 648 3507
Fragments arranged by size:
3507 1243 648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4322 4496 65
Size: 1183 3074 174 1396
Fragments arranged by size:
3074 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2556 2624 4471 4658 1700
Size: 110 746 68 1847 187 2869
Fragments arranged by size:
2869 1847 746 187 110 68
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5827
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5827
BssSI C'ACGA_G
Cuts at: 3887 4899 3887
Size: 1012 4815
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5827
BstBI TT'CG_AA
Cuts at: 4631 4631
Size: 5827
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5827
BstXI CCAn_nnnn'nTGG
Cuts at: 2353 2536 2353
Size: 183 5644
BstYI r'GATC_y
Cuts at: 1972 3157 3524 3909 3926 5378 5464 5476 5822
Size: 1185 367 385 17 1452 86 12 346
Cuts at: 5822 1972
Size: 1977
Fragments arranged by size:
1977 1452 1185 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2898 513
Size: 552 1833 3442
Fragments arranged by size:
3442 1833 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5088
Fragments arranged by size:
5088 683 56
BtsI GCAGTG_nn'
Cuts at: 2973 3343 4137 4157 2973
Size: 370 794 20 4643
Fragments arranged by size:
4643 794 370 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5792
DraI TTT'AAA
Cuts at: 3131 3131
Size: 5827
DraIII CAC_nnn'GTG
Cuts at: 3049 3049
Size: 5827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4834 809
Size: 507 3518 1802
Fragments arranged by size:
3518 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2518 2626 2928
Size: 54 1425 50 275 18 688 108 302
Cuts at: 2928 3274 4163 5792 8
Size: 346 889 1629 43
Fragments arranged by size:
1629 1425 889 688 346 302 275 108
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3274 1537
Size: 275 1462 4090
Fragments arranged by size:
4090 1462 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2480 3757 1353
Size: 1127 1277 3423
Fragments arranged by size:
3423 1277 1127
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4414 4788 4934 387
Size: 1064 138 2825 374 146 1280
Fragments arranged by size:
2825 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2769 2870 3060 3137 3890 4487 5274
Size: 393 1137 101 190 77 753 597 787
Cuts at: 5274 5542 1239
Size: 268 1524
Fragments arranged by size:
1524 1137 787 753 597 393 268 190
101 77
EcoO109I rG'GnC_Cy
Cuts at: 3098 3098
Size: 5827
EcoRI G'AATT_C
Cuts at: 2590 2633 3136 3514 2590
Size: 43 503 378 4903
Fragments arranged by size:
4903 503 378 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 5827
FspI TGC'GCA
Cuts at: 4333 4333
Size: 5827
HaeII r_GCGC'y
Cuts at: 1678 2857 4974 1678
Size: 1179 2117 2531
Fragments arranged by size:
2531 2117 1179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2240 2272 2715 2747 4468
Size: 32 135 32 668 32 443 32 1721
Cuts at: 4468 4500 4542 4574 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1721 668 443 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2363 2509 3171 3417 669
Size: 988 281 425 146 662 246 3079
Fragments arranged by size:
3079 988 662 425 281 246 146
HindIII A'AGCT_T
Cuts at: 748 2465 748
Size: 1717 4110
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KpnI G_GTAC'C
Cuts at: 3152 3152
Size: 5827
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4941 5125 1305
Size: 3636 184 2007
Fragments arranged by size:
3636 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2520 10
Size: 54 1425 343 688 3317
Fragments arranged by size:
3317 1425 688 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 3785 4144 4303 518
Size: 648 1368 1251 359 159 2042
Fragments arranged by size:
2042 1368 1251 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2280 2831 3243 3920 5068 5313 1606
Size: 674 551 412 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 674 551 412 245
NaeI GCC'GGC
Cuts at: 2558 2626 2558
Size: 68 5759
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5275
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5827
NgoMIV G'CCGG_C
Cuts at: 2556 2624 2556
Size: 68 5759
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NspI r_CATG'y
Cuts at: 1132 1229 2110 3185 4730 1132
Size: 97 881 1075 1545 2229
Fragments arranged by size:
2229 1545 1075 881 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5827
PciI A'CATG_T
Cuts at: 2106 4726 2106
Size: 2620 3207
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3049 1108
Size: 1941 3886
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 5409 5441 3896
Size: 32 1481 32 4282
Fragments arranged by size:
4282 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3098 3098
Size: 5827
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5827
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PstI C_TGCA'G
Cuts at: 830 2888 2914 3179 830
Size: 2058 26 265 3478
Fragments arranged by size:
3478 2058 265 26
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
PvuII CAG'CTG
Cuts at: 2280 2831 2280
Size: 551 5276
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SanDI GG'GwC_CC
Cuts at: 3098 3098
Size: 5827
SbfI CC_TGCA'GG
Cuts at: 3179 3179
Size: 5827
ScaI AGT'ACT
Cuts at: 4075 5787 4075
Size: 1712 4115
SfcI C'TryA_G
Cuts at: 826 1045 2884 2910 3175 4310 4991 5182 826
Size: 219 1839 26 265 1135 681 191 1471
Fragments arranged by size:
1839 1471 1135 681 265 219 191 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5827
SmaI CCC'GGG
Cuts at: 1872 3154 1872
Size: 1282 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3931 4832 5094 820
Size: 197 926 1988 901 262 1553
Fragments arranged by size:
1988 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3618 493
Size: 3125 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 3185 1132
Size: 2053 3774
SspI AAT'ATT
Cuts at: 5 52 3751 5
Size: 47 3699 2081
Fragments arranged by size:
3699 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5827
StyI C'CwwG_G
Cuts at: 513 1065 2331 3266 513
Size: 552 1266 935 3074
Fragments arranged by size:
3074 1266 935 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 3003 3854 3871 4024 4209 4654
Size: 1255 558 865 851 17 153 185 445
Cuts at: 4654 325
Size: 1498
Fragments arranged by size:
1498 1255 865 851 558 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4073 5785 96
Size: 274 80 33 51 1443 2096 1712 138
Fragments arranged by size:
2096 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2753 2785 5787 5819 2753
Size: 32 3002 32 2761
Fragments arranged by size:
3002 2761 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5827
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5827
XmaI C'CCGG_G
Cuts at: 1870 3152 1870
Size: 1282 4545
XmnI GAAnn'nnTTC
Cuts at: 3956 3956
Size: 5827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3635 276
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI